当前位置： SCI文献检索 > ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS期刊下所有文献 > Knockdown of pyruvate carboxylase or fatty acid synthase lowers numerous lipids and glucose-stimulated insulin release in insulinoma cells.

Knockdown of pyruvate carboxylase or fatty acid synthase lowers numerous lipids and glucose-stimulated insulin release in insulinoma cells.

Abstract：

:We previously showed that knockdown of the anaplerotic enzyme pyruvate carboxylase in the INS-1 832/13 insulinoma cell line inhibited glucose-stimulated insulin release and glucose carbon incorporation into lipids. We now show that knockdown of fatty acid synthase (FAS) mRNA and protein also inhibits glucose-stimulated insulin release in this cell line. Levels of numerous phospholipids, cholesterol esters, diacylglycerol, triglycerides and individual fatty acids with C14-C24 side chains were acutely lowered about 20% in glucose-stimulated pyruvate carboxylase knockdown cells over a time course that coincides with insulin secretion. In FAS knockdown cells glucose carbon incorporation into lipids and the levels of the subclasses of phospholipids and cholesterol ester species were lower by 20-30% without inhibition of glucose oxidation. These studies suggest that rapid lipid modification is essential for normal glucose-stimulated insulin secretion.

journal_name

Arch Biochem Biophys

journal_title

Archives of biochemistry and biophysics

authors

MacDonald MJ,Hasan NM,Dobrzyn A,Stoker SW,Ntambi JM,Liu X,Sampath H

doi

10.1016/j.abb.2013.01.002

subject

Has Abstract

pub_date

2013-04-01 00:00:00

pages

23-31

issue

1

eissn

0003-9861

issn

1096-0384

pii

S0003-9861(13)00007-6

journal_volume

532

pub_type

杂志文章

相关文献

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS文献大全
  • Stereochemical studies of the C-methylation of deoxycytidine catalyzed by HhaI methylase and the N-methylation of deoxyadenosine catalyzed by EcoRI methylase.

    abstract：:The steric course of methyl group transfer catalyzed by two DNA methylases, HhaI methylase, a DNA (cytosine-5)-methyltransferase, and EcoRI methylase, which methylates at N6 of adenosine, has been studied with (methyl-R)- and (methyl-S)-[methyl-2H1,3H]adenosylmethionine as the methyl donor, using as substrates poly-d(...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(91)90294-s

    authors： Ho DK,Wu JC,Santi DV,Floss HG

    更新日期：1991-02-01 00:00:00

  • Application of quantitative structure-activity relationship modeling to the evaluation of the changes in enzymatic activity of carboxypeptidase Y upon chemical modifications.

    abstract：:A series of 18 phenacyl bromide and iodoacetamide analogues have been synthesized and used to alkylate Met-398 situated in the S'1 binding site of carboxypeptidase Y. The course of the reactions was monitored by measurements of the peptidase and esterase activities. All except four of the reagents reacted selectively,...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1993.1358

    authors： Kanstrup A,Breddam K,Buchardt O

    更新日期：1993-08-01 00:00:00

  • Comparative study of monomeric reconstituted and membrane microsomal monooxygenase systems of the rabbit liver. I. Properties of NADPH-cytochrome P450 reductase and cytochrome P450 LM2 (2B4) monomers.

    abstract：:Oligomers and monomers of NADPH-cytochrome P450 reductase and cytochrome P450 LM2 (2B4) isolated from the liver microsomes of phenobarbital-treated rabbits were examined for physicochemical properties and catalytic activities. As measured using laser correlation spectroscopy the particle sizes of NADPH-cytochrome P450...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(92)90427-x

    authors： Kanaeva IP,Dedinskii IR,Skotselyas ED,Krainev AG,Guleva IV,Sevryukova IF,Koen YM,Kuznetsova GP,Bachmanova GI,Archakov AI

    更新日期：1992-11-01 00:00:00

  • Regulation of the Ca2+-dependent neutral proteinases from rabbit liver by an endogenous inhibitor.

    abstract：:An endogenous inhibitor of neutral Ca2+-dependent proteinases has been isolated from rabbit liver cytosol. The inhibitor is a heat-stable, 240-kDa, tetrameric protein. It is dissociated into its 60-kDa subunits by high concentrations of Ca2+ (0.1-1 mM), but not by lower concentrations in the physiological range. Inhib...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(84)90568-x

    authors： Melloni E,Salamino F,Sparatore B,Michetti M,Pontremoli S,Horecker BL

    更新日期：1984-08-01 00:00:00

  • Inhibition of inorganic pyrophosphatase of animal mitochondria by calcium.

    abstract：:Calcium ion is an uncompetitive inhibitor of the inorganic pyrophosphatases of bovine heart and rat liver mitochondria with respect to substrate MgPPi at pH 8.5 and a non-competitive inhibitor of the former enzyme at pH 7.2. The concentration of Ca2+ required to decrease the maximal velocities for both enzymes at pH 8...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(89)90486-4

    authors： Baykov AA,Volk SE,Unguryte A

    更新日期：1989-09-01 00:00:00

  • Formation of glutathione-conjugated semiquinones by the reaction of quinones with glutathione: an ESR study.

    abstract：:The nonenzymatic reaction of the cytotoxic compounds menadione (2-methyl-1,4-naphthoquinone) and 1,4-naphthoquinone (a reactive metabolite of 1-naphthol) with reducing agents such as NADPH and glutathione led to the formation of semiquinone-free radicals, which were detected with electron spin resonance spectroscopy. ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(87)90006-3

    authors： Takahashi N,Schreiber J,Fischer V,Mason RP

    更新日期：1987-01-01 00:00:00

  • Characterization of the methionine S-oxidase activity of rat liver and kidney microsomes: immunochemical and kinetic evidence for FMO3 being the major catalyst.

    abstract：:Methionine is oxidized to methionine sulfoxide by rat liver and kidney microsomes in an O2- and NADPH-dependent manner. In all microsomal assays, no methionine sulfone was detected. Use of a monoclonal antibody to rat liver cytochrome P-450 reductase, various cytochrome P-450 and peroxidase inhibitors, antioxidants, a...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1996.0370

    authors： Krause RJ,Ripp SL,Sausen PJ,Overby LH,Philpot RM,Elfarra AA

    更新日期：1996-09-01 00:00:00

  • The use of 6-(difluoromethyl)indole to study the activation of indole by tryptophan synthase.

    abstract：:6-(Difluoromethyl)indole has been characterized and developed as a probe for the turnover of indole by the bifunctional enzyme, tryptophan synthase (alpha 2 beta 2). The neutral form of the indolyl species undergoes a slow and spontaneous hydrolysis to produce 6-formylindole with a rate constant (k1) of 0.0089 +/- 0.0...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(91)90068-t

    authors： Woolridge EM,Rokita SE

    更新日期：1991-05-01 00:00:00

  • Lanthanum as a calcium-substituting ion for binding to sarcoplasmic reticulum ATPase.

    abstract：:Ca2+ binding and internalization in sarcoplasmic reticulum ATPase can be investigated by the use of La3+ as a Ca2+ analog. Displacement kinetics of Ca2+ bound by La3+ in native vesicles is a slow biphasic process (k1 = 0.55 s-1 and k2 = 0.05 s-1) that is consistent with the existence of two Ca2+ binding populations wh...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(88)90086-0

    authors： Fernandez-Belda F

    更新日期：1988-12-01 00:00:00

  • Antithrombin in vertebrate species: conservation of the heparin-dependent anticoagulant mechanism.

    abstract：:Heparin is thought to regulate the rate of mammalian blood clotting by enhancing the activity of antithrombin, an inhibitor of coagulation enzymes. The present study establishes that this same inhibitor is present in the blood plasma of each of the terrestrial vertebrate groups including mammals, birds, reptiles, and ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(83)90488-5

    authors： Jordan RE

    更新日期：1983-12-01 00:00:00

  • Enzymatic properties of dimethylglycine dehydrogenase and sarcosine dehydrogenase from rat liver.

    abstract：:Dimethylglycine dehydrogenase (EC 1.5.99.2) and sarcosine dehydrogenase (EC 1.5.99.1) are flavoproteins which catalyze the oxidative demethylation of dimethylglycine to sarcosine and sarcosine to glycine, respectively. During these reactions tightly bound tetrahydropteroylpentaglutamate (H4PteGlu5) is converted to 5,1...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(85)90516-8

    authors： Porter DH,Cook RJ,Wagner C

    更新日期：1985-12-01 00:00:00

  • Translocation of deltaPKC to mitochondria during cardiac reperfusion enhances superoxide anion production and induces loss in mitochondrial function.

    abstract：:Activation of the delta-isoform of protein kinase C (deltaPKC) by certain conditions of oxidative stress results in translocation of the kinase to the mitochondria leading to release of cytochrome c and the induction of apoptosis. In the current study, the effects of myocardial reperfusion-induced deltaPKC translocati...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2005.05.007

    authors： Churchill EN,Szweda LI

    更新日期：2005-07-15 00:00:00

  • The photosynthetic deficiency due to puhC gene deletion in Rhodobacter capsulatus suggests a PuhC protein-dependent process of RC/LH1/PufX complex reorganization.

    abstract：:Optimal photosynthetic reaction centre (RC) and core antenna (LH1) levels in the purple bacterium Rhodobacter capsulatus require the puhC gene. Deletion of puhC had little effect on RC and LH1 assembly individually, but significantly inhibited the photosynthetic growth of RC+ LH1- strains, suggesting that maximal RC c...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2006.07.009

    authors： Aklujkar M,Prince RC,Beatty JT

    更新日期：2006-10-01 00:00:00

  • Induction and superinduction of 2,3,7,8-tetrachlorodibenzo-rho-dioxin-inducible poly(ADP-ribose) polymerase: role of the aryl hydrocarbon receptor/aryl hydrocarbon receptor nuclear translocator transcription activation domains and a labile transcription r

    abstract：:The environmental contaminant 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) induces a novel poly(ADP-ribose) polymerase (TiPARP). In this study, the signaling pathway of the induction was analyzed. Induction of TiPARP by TCDD occurs in both hepa1c1c7 cells and C57 mouse liver. Induction is concentration and time dependen...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/s0003-9861(02)00339-9

    authors： Ma Q

    更新日期：2002-08-15 00:00:00

  • Insulinotropic action of methyl pyruvate: secretory, cationic, and biosynthetic aspects.

    abstract：:Methyl pyruvate was found to exert a dual effect on insulin release from isolated rat pancreatic islets. A positive insulinotropic action prevailed at low concentrations of D-glucose, in the 2.8 to 8.3 mM range, and at concentrations of the ester not exceeding 10.0 mM. It displayed features typical of a process of nut...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1996.0504

    authors： Malaisse WJ,Jijakli H,Ulusoy S,Cook L,Best L,Viñambres C,Villanueva-Peñacarrillo ML,Valverde I,Sener A

    更新日期：1996-11-15 00:00:00

  • The low expression level of pokeweed antiviral protein (PAP) gene in Escherichia coli by the inducible lac promoter is due to inefficient transcription and translation and not to the toxicity of the PAP.

    abstract：:Pokeweed (Phytolacca americana) antiviral protein (PAP) is a glycosidase which inactivates both eukaryotic and prokaryotic ribosomes. Due to this activity the wild-type PAP gene encoding mature protein has not so far been expressed in Escherichia coli. In spite of the ribosome impairing activity of the pre-PAP (contai...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1997.0552

    authors： Xu J,Kaloyanova D,Ivanov IG,AbouHaidar MG

    更新日期：1998-03-01 00:00:00

  • pH dependence of the reaction rate of p-bromophenacyl bromide and of the binding constants of Ca2+ and an amide-type substrate analog to bovine pancreatic phospholipase A2.

    abstract：:pH dependence of the chemical reaction rates of p-bromophenacyl bromide (BPB) and of the binding constants of Ca2+ to bovine pancreatic active- and pro-phospholipases A2 (PLA2s) was studied at 25 degrees C and ionic strength 0.2. The pH dependence curves of the reaction rates of BPB with both enzymes were biphasic. Th...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1998.0681

    authors： Fujii S,Meida M,Tani T,Inoue S,Iwama S,Katsumura S,Ikeda K

    更新日期：1998-06-01 00:00:00

  • Renalase does not catalyze the oxidation of catecholamines.

    abstract：:It is widely accepted that the function of human renalase is to oxidize catecholamines in blood. However, this belief is based on experiments that did not account for slow, facile catecholamine autoxidation reactions. Recent evidence has shown that renalase has substrates with which it reacts rapidly. The reaction cat...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2015.05.016

    authors： Beaupre BA,Hoag MR,Moran GR

    更新日期：2015-08-01 00:00:00

  • Site-directed mutagenesis of cation coordinating residues in the gastric H,K-ATPase.

    abstract：:Site-mutations were introduced into putative cation binding site 1 of the H,K-ATPase at glu-797, thr-825, and glu-938. The side chain oxygen of each was not essential but the mutations produced different activation and inhibition kinetics. Site mutations thr-825 (ala, leu) and glu-938 (ala, gln) modestly decreased the...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.2000.2243

    authors： Rulli SJ,Louneva NM,Skripnikova EV,Rabon EC

    更新日期：2001-03-01 00:00:00

  • Mediterranean diet polyphenols reduce inflammatory angiogenesis through MMP-9 and COX-2 inhibition in human vascular endothelial cells: a potentially protective mechanism in atherosclerotic vascular disease and cancer.

    abstract：:Diets with high content of antioxidant polyphenols are associated with low prevalence of cardiovascular diseases and cancer. Inflammatory angiogenesis is a key pathogenic process both in cancer and atherosclerosis, and is tightly regulated by the proinflammatory enzyme cyclooxygenase (COX)-2 and the matrix degrading e...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2012.05.003

    authors： Scoditti E,Calabriso N,Massaro M,Pellegrino M,Storelli C,Martines G,De Caterina R,Carluccio MA

    更新日期：2012-11-15 00:00:00

  • Myotoxin II from Bothrops asper (Terciopelo) venom is a lysine-49 phospholipase A2.

    abstract：:A basic, dimeric myotoxic protein, myotoxin II, purified from Bothrops asper venom has a similar molecular weight and is immunologically cross-reactive with antibodies raised to previously isolated B. asper phospholipases A2, except that it shows only 0.1% of the phospholipase activity against L-alpha-phosphatidylchol...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(91)90307-5

    authors： Francis B,Gutierrez JM,Lomonte B,Kaiser II

    更新日期：1991-02-01 00:00:00

  • Purification of soluble murine interleukin 5 (IL-5) receptor alpha expressed in Chinese hamster ovary cells and its action as an IL-5 antagonist.

    abstract：:Recombinant soluble murine interleukin 5 receptor alpha (sIL-5R alpha) was expressed in Chinese hamster ovary cells and its biological activity was characterized. By using murine IL-5-coupled Sepharose, the single step purification method of the protein was established. The purified protein inhibited the murine IL-5-i...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1993.1571

    authors： Tsuruoka N,Yamashiro K,Tsujimoto M

    更新日期：1993-11-15 00:00:00

  • The role of cytochrome c and mitochondrial catalase in hydroperoxide-induced heart mitochondrial lipid peroxidation.

    abstract：:The role of cytochrome c and catalase in hydroperoxide-induced lipid peroxidation of rat heart mitochondria was investigated. Mitoplasts were prepared from hearts of aminotriazole-treated rats which displayed both an 80-90% reduction in matrix catalase activity and rate of H2O2 consumption. Catalase-depleted mitochond...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1993.1055

    authors： Radi R,Bush KM,Freeman BA

    更新日期：1993-01-01 00:00:00

  • Isolation and characterization of uteroglobin from the lung of the hare (Lepus capensis).

    abstract：:Uteroglobin has been purified from hare lung by gel filtration and chromatography on carboxymethyl-cellulose. Hare uteroglobin appears homogeneous by electrophoresis under both denaturing and nondenaturing conditions. Its chemical and immunological properties as well as its ability to bind progesterone are compared to...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(83)90323-5

    authors： Soledad López de Haro M,Nieto A

    更新日期：1983-10-15 00:00:00

  • Citrate effects on the Ca2+-loading capacity of isolated rat liver mitochondria: interaction of citrate and ATP.

    abstract：:The maximal amounts of Ca2+ being accumulated (delta Ca2+max) and H+ emitted (delta H+max) by Ca2+-loading mitochondria, with succinate (+rotenone) as respiratory substrate, were evaluated. delta Ca2+max was increased by providing either citrate or ATP to a Pi- and Mg2+-free medium. With citrate, delta H+max was only ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(85)90548-x

    authors： Guidoux R

    更新日期：1985-12-01 00:00:00

  • PSK2 coordinates glucose metabolism and utilization to maintain ultradian clock-coupled respiratory oscillation in Saccharomyces cerevisiae yeast.

    abstract：:Ultradian clock-coupled respiratory oscillation (UCRO) in an aerobic continuous culture of Saccharomyces cerevisiae S288C is principally regulated by control of certain redox reactions of energy metabolism. It is also modulated by the metabolism of storage carbohydrates during adaptation to environmental change. Howev...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2011.02.005

    authors： Ouyang Y,Xu Q,Mitsui K,Motizuki M,Xu Z

    更新日期：2011-05-01 00:00:00

  • Alteration of product formation by directed mutagenesis and truncation of the multiple-product sesquiterpene synthases delta-selinene synthase and gamma-humulene synthase.

    abstract：:Two recombinant sesquiterpene synthases from grand fir, delta-selinene synthase and gamma-humulene synthase, each produce more than 30 sesquiterpene olefins from the acyclic precursor farnesyl diphosphate. These enzymes contain a pair of DDxxD motifs, on opposite lips of the presumptive active site, which are thought ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/S0003-9861(02)00068-1

    authors： Little DB,Croteau RB

    更新日期：2002-06-01 00:00:00

  • Difference between neuronal and nonneuronal (Na+ + K+)-ATPases in their conformational equilibrium.

    abstract：:Several experiments were carried out to study the difference between two isozymes (alpha(+) and alpha) of (Na+ + K+)-ATPase in the conformational equilibrium. Rat brain (Na+ + K+)-ATPase was much more thermolabile than the kidney enzyme. Both enzymes were protected from heat inactivation not only by Na+ and K+, but al...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(88)90643-1

    authors： Matsuda T,Iwata H

    更新日期：1988-06-01 00:00:00

  • Paracrine regulation of neural stem cells in the subependymal zone.

    abstract：:Stem cells maintain their self-renewal and multipotency capacities through a self-organizing network of transcription factors and intracellular pathways activated by extracellular signaling from the microenvironment or "niche" in which they reside in vivo. In the adult mammalian brain new neurons continue to be genera...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章,评审

    doi：10.1016/j.abb.2012.10.001

    authors： Porlan E,Perez-Villalba A,Delgado AC,Ferrón SR

    更新日期：2013-06-01 00:00:00

  • Losartan, an antagonist of AT1 receptor for angiotensin II, attenuates lipopolysaccharide-induced acute lung injury in rat.

    abstract：:Angiotensin II (Ang II) plays an important role in inflammatory process. Acute lung injury (ALI), an inflammatory disorder of the lung, is commonly associated with endotoxemia; however, the mechanism that endotoxin (lipopolysaccharide, LPS) induces the inflammatory response in ALI is not well defined. Here, we showed,...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2008.09.019

    authors： Liu L,Qiu HB,Yang Y,Wang L,Ding HM,Li HP

    更新日期：2009-01-01 00:00:00