Losartan, an antagonist of AT1 receptor for angiotensin II, attenuates lipopolysaccharide-induced acute lung injury in rat.


:Angiotensin II (Ang II) plays an important role in inflammatory process. Acute lung injury (ALI), an inflammatory disorder of the lung, is commonly associated with endotoxemia; however, the mechanism that endotoxin (lipopolysaccharide, LPS) induces the inflammatory response in ALI is not well defined. Here, we showed, in LPS-induced ALI rat model, that Ang II and Ang II type 1 (AT(1)) receptor were significantly increased in lung tissues, compared with those in controls. Meanwhile, nuclear factor (NF)-kappaB-DNA-binding activity, tumor necrosis factor (TNF)-alpha mRNA, and pneumocytic apoptosis were significantly increased. Moreover, pretreatment of rats with losartan, an antagonist of AT(1) receptor for Ang II, improved the inflammation, reduced the elevation of Ang II and AT(1) receptor, and inhibited NF-kappaB-DNA-binding activity, expression of TNF-alpha mRNA, and pneumocytic apoptosis. The data indicate that Ang II may mediate the inflammatory process in LPS-induced ALI through AT(1) receptor, which can be blocked by losartan.


Arch Biochem Biophys


Liu L,Qiu HB,Yang Y,Wang L,Ding HM,Li HP




Has Abstract


2009-01-01 00:00:00














  • Effect of the phosphate substrate on drug-inhibitor binding to human purine nucleoside phosphorylase.

    abstract::The thermodynamics of the drug-inhibitors acyclovir, ganciclovir, and 9-benzylguanine binding to human purine nucleoside phosphorylase (hsPNP) were determined from isothermal titration calorimetry as a function of the substrate phosphate ion (Pi) concentration from 0 to 0.125 M and temperature from 15 degrees C to 35 ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Todorova NA,Schwarz FP

    更新日期:2008-12-15 00:00:00

  • Reductive modification and nonreductive activation of purified spinach chloroplast NADP-dependent glyceraldehyde-3-phosphate dehydrogenase.

    abstract::Spinach chloroplast NAD(P)-glyceraldehyde-3-phosphate dehydrogenase (NAD(P)-GAPDH; EC, was purified as the 600-kDa oligomer of low specific activity. Incubation of the enzyme with either a reductant or a 1,3-bisphosphoglycerate (1,3bisPGA) generating system, but most effectively with both, resulted in an inc...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Baalmann E,Backhausen JE,Rak C,Vetter S,Scheibe R

    更新日期:1995-12-20 00:00:00

  • Characterization of the intracellular and the plasma membrane Ca2+-ATPases in fractionated pig brain membranes using calcium pump inhibitors.

    abstract::The Ca2+-ATPase activity of isolated membranes and purified plasma membrane ATPase from pig brain was measured in the presence of specific inhibitors. The inhibition of the enzymatic activity by vanadate presents a lower affinity in microsomes than in the synaptic plasma membrane vesicles, showing K0.5 of 0.4 and 0.2 ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Salvador JM,Mata AM

    更新日期:1998-03-15 00:00:00

  • Ligand-induced domain movement in pyruvate kinase: structure of the enzyme from rabbit muscle with Mg2+, K+, and L-phospholactate at 2.7 A resolution.

    abstract::The structure of rabbit muscle pyruvate kinase crystallized as a complex with Mg2+, K+, and L-phospholactate (L-P-lactate) has been solved and refined to 2.7 A resolution. The crystals, grown from solutions of polyethylene glycol 8000 at pH 7.5, belong to the space group P2(1) and have unit cell parameters a = 144.4 A...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Larsen TM,Benning MM,Wesenberg GE,Rayment I,Reed GH

    更新日期:1997-09-15 00:00:00

  • Modulation of the reactivity of the thiol of human serum albumin and its sulfenic derivative by fatty acids.

    abstract::The single cysteine residue of human serum albumin (HSA-SH) is the most abundant plasma thiol. HSA transports fatty acids (FA), a cargo that increases under conditions of diabetes, exercise or adrenergic stimulation. The stearic acid-HSA (5/1) complex reacted sixfold faster than FA-free HSA at pH 7.4 with the disulfid...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Torres MJ,Turell L,Botti H,Antmann L,Carballal S,Ferrer-Sueta G,Radi R,Alvarez B

    更新日期:2012-05-01 00:00:00

  • Biochemical evidence for the orientation of cytochrome b in the yeast mitochondrial membrane in the eight-helix model.

    abstract::The topographical localization of the N-terminus of cytochrome b in the inner mitochondrial membrane was determined by mild proteolysis of the yeast mitochondrial cytochrome bc1 complex and identification of the proteolytic fragments derived from subunits of the complex with an established orientation in the inner mem...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Beattie DS,Jenkins HC,Howton MM

    更新日期:1994-07-01 00:00:00

  • Phospholipase C from Clostridium perfringens: preparation and characterization of homogeneous enzyme.

    abstract::A new procedure for the purification of phospholipase C from Clostridium perfringens has been devised that results in essentially pure enzyme. The procedure consists of ammonium sulfate fractionation, ion-exchange chromatography on QAE-Sephadex, and affinity chromatography on phosphatidylcholine linked to Sepharose. T...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Krug EL,Kent C

    更新日期:1984-06-01 00:00:00

  • Glucuronoxylan recognition by GH 30 xylanases: A study with enzyme and substrate variants.

    abstract::XynA from Erwinia chrysanthemi (EcXyn30A), belonging to glycoside hydrolase family 30 subfamily 8, is specialized for hydrolysis of 4-O-methylglucuronoxylan (GX). Carboxyl group of 4-O-methylglucuronic acid serves as a substrate recognition element interacting ionically with positively charged Arg293 of the enzyme. We...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Šuchová K,Kozmon S,Puchart V,Malovíková A,Hoff T,Mørkeberg Krogh KBR,Biely P

    更新日期:2018-04-02 00:00:00

  • The role of the nuclear receptor CAR as a coordinate regulator of hepatic gene expression in defense against chemical toxicity.

    abstract::The nuclear receptor CAR (constitutive active receptor) mediates the induction of transcription of cytochrome P450 (CYP) genes by phenobarbital (PB) and PB-type inducers. A recent study using CAR-null mice has shown that CAR regulates not only the CYP genes but also other genes encoding various drug/steroid-metabolizi...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Yamamoto Y,Kawamoto T,Negishi M

    更新日期:2003-01-01 00:00:00

  • Properties of fructose-1,6-bisphosphate aldolase from Escherichia coli: an NMR analysis.

    abstract::A class II Zn(2+)-dependent fructose-1,6-bisphosphate (FBP)- aldolase was purified from an overproducer strain of Escherichia coli and characterized by standard biochemical techniques and 13C NMR spectroscopy. The principal finding of these studies was identification, by 13C NMR spectroscopy, of an enzyme-bound reacti...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Szwergold BS,Ugurbil K,Brown TR

    更新日期:1995-02-20 00:00:00

  • Static compression is associated with decreased diffusivity of dextrans in cartilage explants.

    abstract::The chondrocytes of adult articular cartilage rely upon transport phenomena within their avascular extracellular matrix for many biological activities. Therefore, changes in matrix structure which influence cytokine transport parameters may be an important mechanism involved in the chondrocyte response to tissue compr...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Quinn TM,Kocian P,Meister JJ

    更新日期:2000-12-15 00:00:00

  • Inhibition of autoxidation of divicine and isouramil by the combination of superoxide dismutase and reduced glutathione.

    abstract::The effects of GSH on the autoxidation of the fava bean pyrimidine aglycones, divicine and isouramil, and on acid-hydrolyzed vicine (provisional identification 2-amino-4,5,6-trihydroxypyrimidine) have been studied. GSH alone promoted redox cycling of each compound, with concomitant GSH oxidation and H2O2 production. I...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Winterbourn CC

    更新日期:1989-06-01 00:00:00

  • A residue-level investigation of the equilibrium unfolding of the C2A domain of synaptotagmin 1.

    abstract::Fibroblast growth factor (FGF) 1 is known to be released in response to cellular stress conditions through formation of a multi-protein complex with synaptotagmin 1 and S100A13. In this study, we characterized the denaturant-induced unfolding of synaptotagmin 1, C2A domain in a residue-specific manner by NMR spectrosc...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Anbazhagan V,Wang HM,Lu CS,Yu C

    更新日期:2009-10-15 00:00:00

  • Analysis of the stability and function of nucleoplasmin through cysteine mutants.

    abstract::Xenopus laevis nucleoplasmin is a pentameric nuclear chaperone. The relation between the structure and the multifunctional aspects of the molecule has not yet been clearly established. In the course of analysing a C-terminally His-tagged recombinant version of the region equivalent to the trypsin resistant core (r-NP1...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Arnan C,Prieto C,Chiva M,Salvany L,Ausió J,Subirana JA,Saperas N

    更新日期:2005-05-15 00:00:00

  • The C-terminal 12 amino acids of sigma(N) are required for structure and function.

    abstract::The sigma(N) protein is an alternative sigma subunit of bacterial RNA polymerase. We investigated the role of a 12-amino-acid "tail" at the C-terminus of Klebsiella pneumoniae sigma(N), which was predicted to be largely surface-exposed and to be mostly loop (that is not alpha-helical or beta-strand). Deletion of this ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Studholme DJ,Finn RD,Chaney MK,Buck M

    更新日期:1999-11-15 00:00:00

  • UDP-galactose 4-epimerase from Escherichia coli: formation of catalytic site during reversible folding.

    abstract::UDP-galactose 4-epimerase from Escherichia coli is a homodimer of molecular weight 39 kDa/subunit having noncovalently bound NAD acting as cofactor. Denaturation by 8 M urea at pH 7.0 causes 85% loss of its secondary structure and dissociation of its constituent molecules. Dilution of the denaturant by buffer at pH 8....

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Barat B,Bhattacharyya D

    更新日期:2001-07-15 00:00:00

  • Reactivity of proteins in ribosomes from Saccharomyces cerevisiae with trypsin.

    abstract::The susceptibility of 40S and 60S ribosomal subunits from Saccharomyces cerevisiae to digestion with varying concentrations of trypsin was studied by two-dimensional electrophoresis and quantitative measurements of the protein remaining with the ribosomal particles after trypsin treatment. Proteins from both subunits ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Lee JC,Haley WT Jr

    更新日期:1983-07-01 00:00:00

  • Turnover of membrane proteins: kinetics of induction and degradation of seven forms of rat liver microsomal cytochrome P-450, NADPH-cytochrome P-450 reductase, and epoxide hydrolase.

    abstract::The in vivo turnover of several rat liver microsomal proteins was studied using techniques designed to maximize antibody recognition specificity and minimize reutilization of radioactive labels. The kinetics of degradation of seven cytochrome P-450 isozymes, NADPH-cytochrome P-450 reductase, and epoxide hydrolase were...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Shiraki H,Guengerich FP

    更新日期:1984-11-15 00:00:00

  • Glyceraldehyde-3-phosphate dehydrogenase inactivation by peroxynitrite.

    abstract::Rabbit muscle glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was inactivated by peroxynitrite under biologically relevant conditions. The decrease of enzymatic activity followed an exponential function, and the concentration of peroxynitrite needed to inactivate 50% of 7 microM GAPDH (IC50) was 17 microM. Hydroxyl r...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Souza JM,Radi R

    更新日期:1998-12-15 00:00:00

  • Sulfhydryl chemistry of Salmonella typhimurium phosphoribosylpyrophosphate synthetase: identification of two classes of cysteinyl residues.

    abstract::Sulfhydryl-specific reagents were used to study the reactivities and function of the four cysteinyl residues per subunit present in Salmonella typhimurium 5-phosphoribosyl-alpha-1-pyrophosphate (PRPP) synthetase. In the presence of high concentrations of denaturants all four cysteinyl residues reacted with sulfhydryl-...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Harlow KW,Switzer RL

    更新日期:1990-02-01 00:00:00

  • Recognition by TNF-alpha of the GPI-anchor glycan induces apoptosis of U937 cells.

    abstract::Tumor necrosis factor-alpha (TNF-alpha) binds to TNF-alpha receptors (TNFR) to produce a hexameric (TNF-alpha)(3)-(TNFR)(3) structure that stimulates apoptosis. We found by using ELISA that TNF-alpha binds to the glycosylphosphatidylinositol (GPI) anchor glycans of carcinoembryonic antigen, human placental alkaline ph...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Fukushima K,Ishiyama C,Yamashita K

    更新日期:2004-06-15 00:00:00

  • Massive production of farnesol-derived dicarboxylic acids in mice treated with the squalene synthase inhibitor zaragozic acid A.

    abstract::The zaragozic acids are potent inhibitors of squalene synthase. In vivo studies in mice confirmed our earlier observations that inhibition of squalene synthase by zaragozic acid A was accompanied by an increase in the incorporation of label from [3H]mevalonate into farnesyl-diphosphate (FPP)-derived isoprenoic acids (...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Vaidya S,Bostedor R,Kurtz MM,Bergstrom JD,Bansal VS

    更新日期:1998-07-01 00:00:00

  • The isolation and characterization of specific 3-methylcholanthrene-binding proteins from rat liver cytosol.

    abstract::The major proteins to which 3-methylcholanthrene specifically binds have been purified over 480-fold with a 45% yield compared to a rat liver 100,000g supernate. The procedure involved a batch ion-exchange technique together with hydrophobic gel filtration and chromatofocusing chromatography. The multiple, specific 3-...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Tierney B,Munzer S,Bresnick E

    更新日期:1983-09-01 00:00:00

  • Interaction of rabbit liver cathepsin M and fructose 1,6-bisphosphatase converting enzyme with their endogenous inhibitors.

    abstract::The stoichiometry of complex formation between two lysosomal proteinases from rabbit liver, cathepsin M and fructose 1,6-bisphosphatase converting enzyme (CE), and their respective endogenous inhibitors was studied by the equilibrium gel penetration method. In each case the molecular weight of the complex was found to...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Pontremoli S,Melloni E,Salamino F,Sparatore B,Michetti M,Horecker BL

    更新日期:1984-02-01 00:00:00

  • Properties and subcellular localization of adenosine diphosphatase in rat heart.

    abstract::Some properties and subcellular localization of adenosine diphosphatase (ADPase) activity from rat heart have been investigated. The pH optimum was 7.4, maximal activity was found with 5 mM MgCl2, and the apparent Km was 20 microM. ADPase activity was strongly inhibited by NaF and AppNHp, and to a lesser extent by AMP...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: de Vente J,Velema J,Zaagsma J

    更新日期:1984-08-15 00:00:00

  • Targeting thioredoxin reductase by plumbagin contributes to inducing apoptosis of HL-60 cells.

    abstract::Plumbagin (PLB), a natural naphthoquinone from the traditional folk medicines Plumbago zeylanica, Dionaea muscipula, or Nepenthes gracilis, has been documented possessing a wide variety of pharmacological activities. Although PLB demonstrates anticancer activity in multiple types of malignant cells, the cellular targe...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Zhang J,Peng S,Li X,Liu R,Han X,Fang J

    更新日期:2017-04-01 00:00:00

  • C-terminal Ser/Pro-Thr-Glu-Leu tetrapeptides of prostaglandin endoperoxide H synthases-1 and -2 target the enzymes to the endoplasmic reticulum.

    abstract::Prostaglandin endoperoxide H synthases-1 and -2 (PGHS-1 and -2) are integral membrane proteins associated with the luminal surface of the endoplasmic reticulum (ER) and the nuclear envelope (NE). The C-terminal sequences of PGHSs end in -Ser/Pro-Thr-Glu-Leu (-S/PTEL), a sequence similar to one of the known ER targetin...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Song I,Smith WL

    更新日期:1996-10-01 00:00:00

  • Stability of the Escherichia coli ATP synthase F0F1 complex is dependent on interactions between gamma Gln-269 and the beta subunit loop beta Asp-301-beta Asp-305.

    abstract::The role of the conserved sequence motif 301DDLTDP306 in the F0F1 ATP synthase beta subunit was assessed by mutagenic analysis in the Escherichia coli enzyme. Mutations gave variable effects on F1 sector activity, stability, and membrane binding to the F0 sector. Upon solubilization, F1 sectors of the betaD302E and be...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Omote H,Tainaka K,Fujie K,Iwamoto-Kihara A,Wada Y,Futai M

    更新日期:1998-10-15 00:00:00

  • Tryparedoxin peroxidase of Leishmania donovani: molecular cloning, heterologous expression, specificity, and catalytic mechanism.

    abstract::Tryparedoxin peroxidase (TXNPx) of Trypanosomatidae is the terminal peroxidase of a complex redox cascade that detoxifies hydroperoxides by NADPH (Nogoceke et al., Biol. Chem. 378, 827-836, 1997). A gene putatively coding for a peroxiredoxin-type TXNPx was identified in L. donovani and expressed in Escherichia coli to...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Flohé L,Budde H,Bruns K,Castro H,Clos J,Hofmann B,Kansal-Kalavar S,Krumme D,Menge U,Plank-Schumacher K,Sztajer H,Wissing J,Wylegalla C,Hecht HJ

    更新日期:2002-01-15 00:00:00

  • Preconditioning potentiates redox signaling and converts death signal into survival signal.

    abstract::Reactive oxygen species (ROS) play a crucial role in the pathophysiology of ischemic heart disease by causing cardiac dysfunction and cell death. Several redox-sensitive anti- and pro-apoptotic transcription factors including NFkappaB and AP-1 progressively and steadily increase in the heart as a function of the durat...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章,评审


    authors: Das DK,Maulik N

    更新日期:2003-12-15 00:00:00