Diphtheria toxin induces leakage of acidic liposomes.

abstract：:Experimental autoimmune encephalomyelitis (EAE) is a commonly used mouse model of multiple sclerosis, a chronic inflammatory disease of the central nervous system (CNS) characterized by demyelination leading to brain and spinal cord malfunctions. We postulate that not only biological but also biomechanical properties ...

journal_title：Archives of biochemistry and biophysics

authors： Pyka-Fościak G,Zemła J,Lis GJ,Litwin JA,Lekka M

更新日期：2020-02-15 00:00:00

abstract：:Glyoxalase I, a member of the metalloglutathione (GSH) transferase superfamily, plays a critical detoxification role in cells by catalyzing the conversion of cytotoxic methylglyoxal (as the diastereomeric GSH-thiohemiacetals) to S-D-lactoylglutathione via a 1,2-hydrogen transfer. The mechanism-of-action of this Zn2+-m...

journal_title：Archives of biochemistry and biophysics

authors： Creighton DJ,Hamilton DS

更新日期：2001-03-01 00:00:00

abstract：:Application of nanosecond pulsed electric fields (nsPEFs) has attracted attention as a unique tool in life sciences, especially for cancer therapy, but the molecular mechanism of its action on living organisms is yet to be fully elucidated. Here, we report a transient activation of signaling pathways involving mitogen...

journal_title：Archives of biochemistry and biophysics

authors： Morotomi-Yano K,Akiyama H,Yano K

更新日期：2011-11-01 00:00:00

abstract：:Solubilized NADPH-cytochrome c (P450) reductase was purified to homogeneity from an extract of spearmint (Mentha spicata) glandular trichomes by dye-ligand interaction chromatography on Matrex-Gel Red A and affinity chromatography on 2', 5'-adenosine diphosphate agarose. SDS-PAGE of the purified enzyme preparation rev...

journal_title：Archives of biochemistry and biophysics

authors： Ponnamperuma K,Croteau R

更新日期：1996-05-01 00:00:00

abstract：:Five functional isoforms of a novel plant thiol methyltransferase from the leaves of cabbage (Brassica oleracea L.) were purified to electrophoretic homogeneity. Pooled, partly purified preparations of the enzyme were previously shown to methylate thiol compounds released upon the hydrolysis of glucosinolates. The enz...

journal_title：Archives of biochemistry and biophysics

authors： Attieh J,Sparace SA,Saini HS

更新日期：2000-08-15 00:00:00

abstract：:The effects of glycosylation on the stability and subunit interactions of vicilin, the major storage protein in pea seeds, were investigated. Glycosylated vicilin derivatives were prepared by alkylation of lysine epsilon-amino groups with various carbohydrates. Average modification levels of 13.4 +/- 3.0, 11.1 +/- 3.6...

journal_title：Archives of biochemistry and biophysics

authors： Pedrosa C,De Felice FG,Trisciuzzi C,Ferreira ST

更新日期：2000-10-15 00:00:00

abstract：:Intrinsically disordered proteins represent about one third of eukaryotic proteins. An additional third correspond to proteins containing folded domains as well as large intrinsically disordered regions (IDR). While IDRs may represent functionally autonomous domains, in some instances it has become clear that they pro...

journal_title：Archives of biochemistry and biophysics

authors： Arbesú M,Pons M

更新日期：2019-11-30 00:00:00

abstract：:L-Tryptophan is an essential amino acid for mammals. It is utilized not only for protein synthesis but also for the biosynthesis of serotonin and melatonin by the serotonin pathway as well as nicotinamide adenine dinucleotide by the kynurenine pathway. Although the kynurenine pathway is responsible for the catabolism ...

journal_title：Archives of biochemistry and biophysics

authors： Geng J,Liu A

更新日期：2014-02-15 00:00:00

abstract：:The pH gradient, delta pH, present across the inner mitochondrial membrane in isolated rat hepatocytes was continuously monitored with a novel spectroscopic technique that utilizes the weak acid fluorescein. Unlike most cytosolic pH indicators, such as 2',7'-bis(carboxyethyl)-5,(6)-carboxyfluorescein (BCECF), fluoresc...

journal_title：Archives of biochemistry and biophysics

authors： Thomas PJ,Gaspers LD,Pharr C,Thomas JA

更新日期：1991-07-01 00:00:00

abstract：:Chlorite dismutase-like proteins are structurally closely related to functional chlorite dismutases which are heme b-dependent oxidoreductases capable of reducing chlorite to chloride with simultaneous production of dioxygen. Chlorite dismutase-like proteins are incapable of performing this reaction and their biologic...

journal_title：Archives of biochemistry and biophysics

authors： Hofbauer S,Hagmüller A,Schaffner I,Mlynek G,Krutzler M,Stadlmayr G,Pirker KF,Obinger C,Daims H,Djinović-Carugo K,Furtmüller PG

更新日期：2015-05-15 00:00:00

abstract：:Methylibium petroleiphilum strain PM1 uses various petroleum products including the fuel additive methyl tert-butyl ether and straight chain and aromatic hydrocarbons as sole carbon and energy sources. It has two operons, dmpI and dmpII, that code for the enzymes in a pair of parallel meta-fission pathways. In order t...

journal_title：Archives of biochemistry and biophysics

authors： Terrell CR,Burks EA,Whitman CP,Hoffman DW

更新日期：2013-09-01 00:00:00

abstract：:Developmentally regulated GTP-binding protein (DRG) is a new subfamily within the superfamily of GTP-binding proteins. Its expression is regulated during embryonic development. To investigate the effect of the expression of DRG2 on cell growth, we constructed a human Jurkat-T-cell line that overexpresses DRG2. Overexp...

journal_title：Archives of biochemistry and biophysics

authors： Ko MS,Lee UH,Kim SI,Kim HJ,Park JJ,Cha SJ,Kim SB,Song H,Chung DK,Han IS,Kwack K,Park JW

更新日期：2004-02-15 00:00:00

abstract：:Diglycyl triperoxodivanadate [V2O2(O2)3(Gly H)2(H2O)2], a synthetic compound with mu-peroxo-bridge derived from H2O2 and vanadate, oxidized bromide to a bromination-competent intermediate in phosphate buffer and physiological pH. This is in contrast to the requirement of acid medium with H2O2 as the oxidant. Addition ...

journal_title：Archives of biochemistry and biophysics

authors： Rao AV,Islam NS,Ramasarma T

更新日期：1997-06-15 00:00:00

abstract：:The effect of CAM [cyclic AMP, Mg-ATP, and 3-isobutyl, 1-methylxanthine (MIX)] on triacylglycerol (TG) lipase activity in extracts from heparin-perfused rat heart was determined. TG lipase activity in homogenate, 10,000g supernatant, 105,000g supernatant, ammonium sulfate supernatant, and the eluate from heparin-Sepha...

journal_title：Archives of biochemistry and biophysics

authors： Palmer WK,Caruso RA,Oscai LB

更新日期：1986-09-01 00:00:00

abstract：:gamma-Synuclein is a small cytoplasmic protein implicated in neurodegenerative diseases and cancer. However, the mechanism of its involvement in diseases is not clear. We studied the role of gamma-synuclein in the regulation of matrix metalloproteinases in retinoblastoma cell culture. Matrix metalloproteinases play im...

journal_title：Archives of biochemistry and biophysics

authors： Surgucheva IG,Sivak JM,Fini ME,Palazzo RE,Surguchov AP

更新日期：2003-02-01 00:00:00

abstract：:The interaction of 5'-deoxy-5'-thioadenosine 5'-monophosphate (A(S)MP) and 5'-deoxy-5'-thioinosine 5'-monophosphate (I(S)MP) with snake venom, 5'nucleotidase, and calf intestinal mucosa alkaline phosphatase has been characterized. The substrates, A(S)MP and I(S)MP, are analogs of adenosine 5'-monophosphate and inosine...

journal_title：Archives of biochemistry and biophysics

authors： Rossomando EF,Cordis GA,Markham GD

更新日期：1983-01-01 00:00:00

abstract：:During the metabolism of 1,2-dimethylhydrazine (a colon carcinogen) in rats, methyl radicals (.CH3) are produced. To gain an insight into the nature of .CH3 interactions with biomolecules in vivo, we conducted in vitro studies using RNA as a model compound. RNA was incubated in a system where .CH3 was generated from t...

journal_title：Archives of biochemistry and biophysics

authors： Kang JO,Gallagher KS,Cohen G

更新日期：1993-10-01 00:00:00

abstract：:Human alcohol dehydrogenases (ADH1 and ADH4) actively use retinoids oxidized at the cyclohexenyl ring (4-oxo-, 4-hydroxy-, and 3,4-didehydro-retinoids), which are functional compounds in several cells and tissues (i.e., in human skin). Remarkably, activities with 4-oxo-retinal and 4-hydroxy-retinol (kcat = 2050 min(-1...

journal_title：Archives of biochemistry and biophysics

authors： Martras S,Alvarez R,Gallego O,Domínguez M,de Lera AR,Farrés J,Parés X

更新日期：2004-10-15 00:00:00

abstract：:Triacylglycerol plays a critical role in an organism's ability to withstand fuel deprivation, and dysregulation of triacylglycerol synthesis is important in the development of diseases such as obesity and diabetes. Glycerol-3-phosphate acyltransferase (GPAT) catalyzes the initial and committed step of glycerolipid syn...

journal_title：Archives of biochemistry and biophysics

authors： Lewin TM,Granger DA,Kim JH,Coleman RA

更新日期：2001-12-01 00:00:00

abstract：:The binding site for ATP to tubulin was established by use of the photoaffinity label [gamma-32P]N3ATP. Photolysis of the analog in the presence of tubulin resulted in covalent modification of the protein as revealed by autoradiography of electropherograms. Scanning the autoradiograms showed that the ATP analog was bo...

journal_title：Archives of biochemistry and biophysics

authors： Zabrecky JR,Cole RD

更新日期：1983-09-01 00:00:00

abstract：:The reaction mechanism of selenocystine beta-lyase has been studied and it was found that elemental selenium is released enzymatically from selenocysteine, and reduced to H2Se nonenzymatically with dithiothreitol or some other reductants that are added to prepare selenocysteine from selenocystine in the anaerobic reac...

journal_title：Archives of biochemistry and biophysics

authors： Esaki N,Karai N,Nakamura T,Tanaka H,Soda K

更新日期：1985-05-01 00:00:00

abstract：:Parazoanthoxanthin A is a fluorescent yellow nitrogenous pigment of the group of zoanthoxanthins, which show a broad range of biological activity. These include, among others, the ability to bind to DNA. In this study we have used a variety of spectroscopic (intrinsic fluorescence emission and UV-spectroscopy) and hyd...

journal_title：Archives of biochemistry and biophysics

authors： Pasic L,Sepcic K,Turk T,Macek P,Poklar N

更新日期：2001-09-01 00:00:00

abstract：:The oxidized cytochrome c(2) from the purple phototrophic bacteria, Rhodobacter sphaeroides and Rhodobacter capsulatus, bind the neutral species of imidazole (K(a) = 1440 +/- 40 M(-1)) 50 times more strongly than does horse mitochondrial cytochrome c (K(a) = 30 +/- 1 M(-1)). The kinetics of imidazole binding are consi...

journal_title：Archives of biochemistry and biophysics

authors： Dumortier C,Meyer TE,Cusanovich MA

更新日期：1999-11-15 00:00:00

abstract：:In a precedent report we showed that alpha-bisabolol, a sesquiterpene present widely in the plant kingdom, exerts a rapid and efficient apoptosis-inducing action selectively towards human and murine malignant glioblastoma cell lines through mitochondrial damage. The present study extends these data demonstrating the a...

journal_title：Archives of biochemistry and biophysics

authors： Darra E,Abdel-Azeim S,Manara A,Shoji K,Maréchal JD,Mariotto S,Cavalieri E,Perbellini L,Pizza C,Perahia D,Crimi M,Suzuki H

更新日期：2008-08-15 00:00:00

abstract：:It was found that the rate of polymerization of G-actin increased with the decrease of ATP concentration. When excess ATP was replaced by chloride through anion-exchange treatment, the extent of actin polymerization did not change provided that the ionic strength was raised immediately after the treatment. In the mean...

journal_title：Archives of biochemistry and biophysics

authors： Fung BM,Eyob E

更新日期：1983-02-01 00:00:00

abstract：:A10 smooth muscle cells, derived from embryonic rat thoracic aorta, responded to the atrial natriuretic factor (ANF) with increased levels of cyclic GMP. These cells possess high-affinity (apparent Kd = 50 pM) plasma membrane receptors for ANF. Internalization of ANF at 37 degrees C was indicated by the following: app...

journal_title：Archives of biochemistry and biophysics

authors： Napier MA,Arcuri KE,Vandlen RL

更新日期：1986-08-01 00:00:00

abstract：:The interactions of Cu, Zn superoxide dismutase (SOD) with nitroxyl (NO-) and nitric oxide (NO), both of which are thought to be biologically significant, have been studied but remain undefined. Having previously noted that NO- can reduce Cu (II), Zn SOD aerobically, we now report that it also can do so anaerobically ...

journal_title：Archives of biochemistry and biophysics

authors： Liochev SI,Fridovich I

更新日期：2002-06-15 00:00:00

abstract：:The key technique in proteome analysis is high-resolution two-dimensional (2D) electrophoretic separation of proteins from biological samples. This method combines isoelectric focusing (IEF) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Derivatization of protein carbonyls with 2, 4-dinitrop...

journal_title：Archives of biochemistry and biophysics

authors： Reinheckel T,Körn S,Möhring S,Augustin W,Halangk W,Schild L

更新日期：2000-04-01 00:00:00

abstract：:Polyunsaturated fatty acids (PUFAs) are reported to exert beneficial effects in Alzheimer's disease. Some PUFAs are known to reduce amyloid-beta (Aβ) toxicity by promoting its degradation and clearance. Studies on the direct interactions of PUFAs with Aβ peptides are limited and contradictory. In this study, we report...

journal_title：Archives of biochemistry and biophysics

authors： El Shatshat A,Pham AT,Rao PPN

更新日期：2019-03-15 00:00:00

abstract：:Cytochrome P4502E (P4502E), the major ethanol-inducible P450 metabolizes ethanol to acetaldehyde and bioactivates procarcinogens to ultimate carcinogens. Metabolism of ethanol to acetaldehyde in the brain could be deleterious since it can react with cytoskeletal proteins, forming adducts. In the present study, rats we...

journal_title：Archives of biochemistry and biophysics

authors： Upadhya SC,Tirumalai PS,Boyd MR,Mori T,Ravindranath V

更新日期：2000-01-01 00:00:00