Cloning, sequencing, and expression of the dipeptidyl peptidase IV gene from Flavobacterium meningosepticum in Escherichia coli.

abstract：:The Ah receptor from rat liver has been purified, using a specific oligonucleotide affinity column, in order to characterize the components of the receptor and to investigate features that modulate its DNA-binding activity. The purified DNA-binding form of rat Ah receptor contains three major components, with estimate...

journal_title：Archives of biochemistry and biophysics

authors： Ireland RC,Li SY,Dougherty JJ

更新日期：1995-06-01 00:00:00

abstract：:Under physiological conditions the uptake of transferrin-bound iron by reticulocytes involves transferrin binding to membrane receptors followed by endocytosis, release of iron from the transferrin within endosomes, and recycling of apotransferrin to the cell surface. However, as shown in the present work, iron uptake...

journal_title：Archives of biochemistry and biophysics

authors： Hodgson LL,Quail EA,Morgan EH

更新日期：1994-01-01 00:00:00

abstract：:Adriamycin (AdM) and related anthracyclines are potent antineoplastic agents, the clinical utility of which is limited by severe cardiotoxicity. Aglycone derivatives of AdM have recently been reported to trigger the release of Ca2+ from isolated, preloaded rat heart mitochondria and to modify mitochondrial sulfhydryl ...

journal_title：Archives of biochemistry and biophysics

authors： Sokolove PM

更新日期：1991-02-01 00:00:00

abstract：:A cDNA that encodes the human plasminogen (HPg) amino acid sequence has been inserted adjacent to the polyhedrin promoter in the genome of the baculovirus, Autographa californica nuclear polyhedrosis virus, which was then used to infect cultured cells of the farm armyworm, Spodoptera frugiperda. Under the conditions o...

journal_title：Archives of biochemistry and biophysics

authors： Whitefleet-Smith J,Rosen E,McLinden J,Ploplis VA,Fraser MJ,Tomlinson JE,McLean JW,Castellino FJ

更新日期：1989-06-01 00:00:00

abstract：:Lipophosphoglycan (LPG) is the major surface glycoconjugate of Leishmania donovani promastigotes and is composed of a capped polymer of repeating PO4-6Gal(beta 1,4)Man alpha 1 disaccharide units linked via a phosphosaccharide core to a lyso-1-O-alkylphosphatidylinositol anchor. An exogenous acceptor composed of the gl...

journal_title：Archives of biochemistry and biophysics

authors： Carver MA,Turco SJ

更新日期：1992-06-01 00:00:00

abstract：:Cofactor and tryptophan accessibility of the 65-kDa form of rat brain glutamate decarboxylase (GAD) was investigated by fluorescence quenching measurements using acrylamide, I-, and Cs+ as the quenchers. Trp residues were partially exposed to solvent. I- was less able and Cs+ was more able to quench the fluorescence o...

journal_title：Archives of biochemistry and biophysics

authors： Rust E,Martin DL,Chen CH

更新日期：2001-08-15 00:00:00

abstract：:Rat liver microsomes catalyze a vanadate-stimulated oxidation of NAD(P)H, which is augmented by paraquat and suppressed by superoxide dismutase, but not by catalase. NADPH oxidation was a linear function of the concentration of microsomes in the absence of vanadate, but was a saturating function in the presence of van...

journal_title：Archives of biochemistry and biophysics

authors： Liochev S,Fridovich I

更新日期：1986-10-01 00:00:00

abstract：:Modulation of CYP3A1 and CYP3A2 mRNA expression by dexamethasone and by phenobarbital has been studied in immature (21-day-old) and adult (90-day-old) rat liver. Positive modulation of these forms by both agents markedly declines with the age of the animals. However, CYP3A2 mRNA, although physiologically extinguished ...

journal_title：Archives of biochemistry and biophysics

authors： Telhada MB,Pereira TM,Lechner MC

更新日期：1992-11-01 00:00:00

abstract：:Interactions between loops 2, 2' and 4, known as the loop bundle, stabilize the active site of caspase-3. Loop 4 (L4) is of particular interest due to its location between the active site and the dimer interface. We have disrupted a salt bridge between K242 and E246 at the base of L4 to determine its role in overall c...

journal_title：Archives of biochemistry and biophysics

authors： Walters J,Swartz P,Mattos C,Clark AC

更新日期：2011-04-01 00:00:00

abstract：:In the presence of glucose and ample oxygen, insect form African trypanosomes release pyruvate more than 100-fold more slowly than do bloodstream forms. This rate decrease could not be accounted for simply by an increased mitochondrial pyruvate oxidation rate as inhibiting mitochondrial respiration increases pyruvate ...

journal_title：Archives of biochemistry and biophysics

authors： Barnard JP,Pedersen PL

更新日期：1994-08-15 00:00:00

abstract：:We have developed a protocol to purify apo-ovine (o) prostaglandin endoperoxide H(2) synthase-1 (PGHS-1) to homogeneity from ram seminal vesicles. The resulting apo enzyme can then be reconstituted with Co(3+)-protoporphyrin IX instead of Fe(3+)-protoporphyrin IX to produce a native-like, but functionally inert, enzym...

journal_title：Archives of biochemistry and biophysics

authors： Malkowski MG,Theisen MJ,Scharmen A,Garavito RM

更新日期：2000-08-01 00:00:00

abstract：:Lipoxygenase is suggested to be involved in the early event of atherosclerosis by inducing plasma low-density lipoprotein (LDL) oxidation in the subendothelial space of the arterial wall. Since flavonoids such as quercetin are recognized as lipoxygenase inhibitors and they occur mainly in the glycoside form, we assess...

journal_title：Archives of biochemistry and biophysics

authors： Luiz da Silva E,Tsushida T,Terao J

更新日期：1998-01-15 00:00:00

abstract：:The effects of benzyl (BITC) and phenethyl isothiocyanate (PEITC) on the activity of a P450 2E1 mutant where the conserved threonine at position 303 was replaced with an alanine residue (P450 2E1 T303A) were examined. PEITC inactivated the mutant enzyme with a K(I) of 1.6 microM. PEITC also inactivated the wild-type P...

journal_title：Archives of biochemistry and biophysics

authors： Moreno RL,Goosen T,Kent UM,Chung FL,Hollenberg PF

更新日期：2001-07-01 00:00:00

abstract：:The increase in crosslinking in normal and nonenzymatically glycosylated rat tail tendon collagen after treatment with decomposing lipid hydroperoxides was assessed by measuring the breaking time of tendons immersed in 7 M urea under a 3 g weight at 40 degrees C (thermal rupture time). The incubation of tendons in 200...

journal_title：Archives of biochemistry and biophysics

authors： Hicks M,Delbridge L,Yue DK,Reeve TS

更新日期：1989-01-01 00:00:00

abstract：:Comparative studies of 5-aminoimidazole ribonucleotide (AIR) carboxylases from Escherichia coli and Gallus gallus have identified this central step in de novo purine biosynthesis as a case for unusual divergence in primary metabolism. Recent discoveries establish the fungal AIR carboxylase, encoded by the ADE2 gene, a...

journal_title：Archives of biochemistry and biophysics

authors： Firestine SM,Misialek S,Toffaletti DL,Klem TJ,Perfect JR,Davisson VJ

更新日期：1998-03-01 00:00:00

abstract：:In the present study, the activity of Topoisomerase IIβ (TopoIIβ) is evaluated during peroxide induced double stranded DNA breaks (DSBs) repair in primary neurons. The results showed that the TopoIIβ levels were enhanced during recovery from peroxide mediated damage (PED) along with Ku70, PARP-1, pol beta, and WRN hel...

journal_title：Archives of biochemistry and biophysics

authors： Mandraju R,Chekuri A,Bhaskar C,Duning K,Kremerskothen J,Kondapi AK

更新日期：2011-12-15 00:00:00

abstract：:Phospholipase C-gamma1, a substrate for many growth factor receptor and nonreceptor tyrosine kinases, produces second messenger molecules that are elements of signal transduction pathways related to cell proliferation. The influence of deletion mutations, which do not intrude on the domains required for catalytic func...

journal_title：Archives of biochemistry and biophysics

authors： Horstman DA,Chattopadhyay A,Carpenter G

更新日期：1999-01-01 00:00:00

abstract：:2,2'-Anhydro-3'-deoxy-5-ethyluridine, a new pyrimidine nucleoside analog, has been examined in terms of its binding potency to uridine phosphorylase, and its conformation in solution (NMR) was studied. 2,2'-Anhydro-3'-deoxy-5-ethyluridine has a Ki value of 3.4 microM for uridine phosphorylase from rat intestinal mucos...

journal_title：Archives of biochemistry and biophysics

authors： Veres Z,Neszmélyi A,Szabolcs A,Kiss AI,Dénes G

更新日期：1991-04-01 00:00:00

abstract：:Plant 3-hydroxy-3-methylglutaryl-CoA reductase(HMGR; EC 1.1.1.34) and sucrose-phosphate synthase (SPS; EC 2.4.1.14) and synthetic peptides designed from the known phosphorylation sites of plant HMGR (SAMS*: KSHMKYNRSTKDVK), rat acetyl-CoA carboxylase (SAMS: HMRSAMSGLHLVKRR), spinach SPS (SP2: GRRJRRISSVEJJDKK), and sp...

journal_title：Archives of biochemistry and biophysics

authors： Toroser D,Huber SC

更新日期：1998-07-15 00:00:00

abstract：:In the liver of the fasted rat, the aldolase B (AldB) mRNA level decreased to about half of that of the control rat. When the control rat was refed the glucose-rich diet, the AldB mRNA level increased about six to seven times more than in the fasted rat. This increase was shown as the activation of the AldB gene trans...

journal_title：Archives of biochemistry and biophysics

authors： Gomez PF,Ito K,Huang Y,Otsu K,Kuzumaki T,Ishikawa K

更新日期：1994-11-01 00:00:00

abstract：:Flavin-dependent monooxygenases catalyze a wide variety of redox reactions in important biological processes and are responsible for the synthesis of highly complex natural products. Although much has been learned about FMO chemistry in the last ~80 years of research, several aspects of the reactions catalyzed by thes...

journal_title：Archives of biochemistry and biophysics

authors： Reis RAG,Li H,Johnson M,Sobrado P

更新日期：2021-01-16 00:00:00

abstract：:Catalases are biotechnologically relevant enzymes because of their applications in food technology, bioremediation, and biomedicine. The dismutation of hydrogen peroxide occurs in two steps; in the first one, the enzyme forms an oxidized compound I (Cpd I) and in the second one, the enzyme is reduced to the ferric sta...

journal_title：Archives of biochemistry and biophysics

authors： Zárate-Romero A,Stojanoff V,Cohen AE,Hansberg W,Rudiño-Piñera E

更新日期：2019-05-15 00:00:00

abstract：:Both CYP158A1 and CYP158A2 are able to catalyze an oxidative C-C coupling reaction producing biflaviolin or triflaviolin in Streptomyces coelicolor A3(2). The substrate-bound crystal structures of CYP158A2 and CYP158A1 reveal that the side chain of Ile87 in CYP158A2 points to the active site contacting the distal flav...

journal_title：Archives of biochemistry and biophysics

authors： Zhao B,Bellamine A,Lei L,Waterman MR

更新日期：2012-02-15 00:00:00

abstract：:Uridine phosphorylase is one of the critical enzymes in the pyrimidine salvage pathway. Cells regenerate uridine for nucleotide metabolism by incorporating uracil with ribose-1-phosphate with this enzyme. Recent studies indicate that Escherichia coli uridine phosphorylase is destabilized in the presence of ATP. Howeve...

journal_title：Archives of biochemistry and biophysics

authors： Liu YK,Lin TH,Liu PF

更新日期：2017-11-15 00:00:00

abstract：:Diethyl dithiocarbamate (DDC) was used to inhibit the copper- and zinc- containing superoxide dismutase (Cu,ZnSOD) of Escherichia coli in order to expose its physiological function. DDC inhibited the aerobic growth of a sodA sodB mutant much more than it did the growth of a SOD-replete parental strain and this inhibit...

journal_title：Archives of biochemistry and biophysics

authors： Benov L,Fridovich I

更新日期：1996-03-15 00:00:00

abstract：:The oxidation of quercetin by horseradish peroxidase/H(2)O(2) was studied in the absence but especially also in the presence of glutathione (GSH). HPLC analysis of the reaction products formed in the absence of GSH revealed formation of at least 20 different products, a result in line with other studies reporting the ...

journal_title：Archives of biochemistry and biophysics

authors： Awad HM,Boersma MG,Vervoort J,Rietjens IM

更新日期：2000-06-15 00:00:00

abstract：:Much has been written recently on osteoclast biology, but this cell type still astonishes scientists with its multifaceted functions and unique properties. The last three decades have seen a change in thinking about the osteoclast, from a cell with a single function, which just destroys the tissue it belongs to, to an...

journal_title：Archives of biochemistry and biophysics

authors： Cappariello A,Maurizi A,Veeriah V,Teti A

更新日期：2014-11-01 00:00:00

abstract：:Cytochrome P4502E (P4502E), the major ethanol-inducible P450 metabolizes ethanol to acetaldehyde and bioactivates procarcinogens to ultimate carcinogens. Metabolism of ethanol to acetaldehyde in the brain could be deleterious since it can react with cytoskeletal proteins, forming adducts. In the present study, rats we...

journal_title：Archives of biochemistry and biophysics

authors： Upadhya SC,Tirumalai PS,Boyd MR,Mori T,Ravindranath V

更新日期：2000-01-01 00:00:00

abstract：:Sequence analyses of the C-terminal membrane intercalative region of the rat cytochrome b(5) indicated that this domain has, in addition to a signal sequence, a combined element of the classic stop-transfer sequence typically found in a variety of transmembrane proteins. Such bitopic protein arrangements arise by tand...

journal_title：Archives of biochemistry and biophysics

authors： Kaderbhai MA,Morgan R,Kaderbhai NN

更新日期：2003-04-15 00:00:00

abstract：:Low-density lipoprotein receptor-related protein (LRP)/alpha 2-macroglobulin receptor is a member of the low-density lipoprotein receptor family. It is known to bind a wide variety of unrelated ligands including alpha 2-macroglobulin-proteinase complexes, tissue plasminogen activator, apolipoprotein E-enriched very lo...

journal_title：Archives of biochemistry and biophysics

authors： Wu SM,Pizzo SV

更新日期：1996-02-01 00:00:00