Dissociation of enzymatic and pharmacological properties of piratoxins-I and -III, two myotoxic phospholipases A2 from Bothrops pirajai snake venom.

abstract：:A lens epithelial cell line established from a transgenic mouse synthesizes high levels of the enzyme aldose reductase which converts sugars to polyols. This enzyme has been implicated in the formation of sugar cataracts in animals and with diabetic complications in man. The mouse aldose reductase has been characteriz...

journal_title：Archives of biochemistry and biophysics

authors： Russell P,Yamada T,Merola LO

更新日期：1990-01-01 00:00:00

abstract：:A microsomal retinol dehydrogenase (RoDH) that recognizes holo-cellular retinol binding protein (CRBP) as substrate is inhibited by phenylarsine oxide (IC50 = 3 microM) in the presence of 10 mM cysteine. Inhibition was reversible with dithiothreitol, indicating that two cysteine residues in close proximity are essenti...

journal_title：Archives of biochemistry and biophysics

authors： Boerman MH,Napoli JL

更新日期：1995-08-20 00:00:00

abstract：:Seven carboxylesterase isozymes were purified to electrophoretic homogeneity from liver microsomes of mouse, hamster, guinea pig, rabbit, and monkey by the same procedure used previously to obtain three isozymes from the rat, and their physical, enzymological, and immunological properties were compared with those of t...

journal_title：Archives of biochemistry and biophysics

authors： Hosokawa M,Maki T,Satoh T

更新日期：1990-03-01 00:00:00

abstract：:Two novel medicinally important legume lectins from Bauhinia purpurea (BPL) and Wisteria floribunda (WFL) possessing extended sugar binding site were investigated for functional and conformational transitions using biochemical and biophysical techniques as well as bioinformatical tools. Homology model of BPL was const...

journal_title：Archives of biochemistry and biophysics

authors： Agrawal SB,Ghosh D,Gaikwad SM

更新日期：2019-02-15 00:00:00

abstract：:Cellular distribution in rat livers of the mannan-binding protein, a liver lectin specific for mannose and N-acetylglucosamine was investigated. Estimation of mannan binding activity of isolated cells demonstrated that more than 99% of the mannan binding activity recovered from whole livers was derived from hepatocyte...

journal_title：Archives of biochemistry and biophysics

authors： Mori K,Kawasaki T,Yamashina I

更新日期：1983-04-15 00:00:00

abstract：:The reactivities of the sulfhydryl groups of rat, turkey, human, and calf hemoglobin were studied together with the enzyme activities of glutathione peroxidase, glutathione reductase, glucose-6-phosphate dehydrogenase, and glutaredoxin in lysed erythrocytes to evaluate their roles in regulating redox homeostasis. The ...

journal_title：Archives of biochemistry and biophysics

authors： Di Simplicio P,Cacace MG,Lusini L,Giannerini F,Giustarini D,Rossi R

更新日期：1998-07-15 00:00:00

abstract：:The effect of ligating the alpha2-macroglobulin signaling receptor (alpha2MSR) with receptor-recognized forms of alpha2M (alpha2M*) was studied with respect to phospholipase D (PLD) activity in murine macrophages, their plasma membranes, and nuclei. PLD activity in plasma membranes and nuclei increased linearly up to ...

journal_title：Archives of biochemistry and biophysics

authors： Misra UK,Pizzo SV

更新日期：1999-03-01 00:00:00

abstract：:Oxidative stress in the small intestine can result in altered cell proliferation, migration, and differentiation of villus-crypt cells. Retinoid metabolism is recognized as an important mediator of cellular differentiation in the intestine. This study examined the effect of oxidative stress in retinoid metabolism in a...

journal_title：Archives of biochemistry and biophysics

authors： Prabhu R,Thomas S,Balasubramanian KA

更新日期：2005-02-15 00:00:00

abstract：:Solar radiation is one of the most important environmental stress agents for human skin, causing sunburn, premature skin aging, and skin cancer. Beta-carotene is discussed to protect against photooxidative stress and thus prevent skin damage. Though beta-carotene has been successfully used against photosensitivity in ...

journal_title：Archives of biochemistry and biophysics

authors： Biesalski HK,Obermueller-Jevic UC

更新日期：2001-05-01 00:00:00

abstract：:An improved high yielding procedure for the purification of myrosinase from Sinapis alba L. consisting of concanavalin A affinity chromatography followed by a chromatofocusing step is presented. The purified enzyme was homogeneous as judged by sodium dodecyl sulfate-gel electrophoresis and by analytical ultracentrifug...

journal_title：Archives of biochemistry and biophysics

authors： Pessina A,Thomas RM,Palmieri S,Luisi PL

更新日期：1990-08-01 00:00:00

abstract：:Short, strong (low barrier) hydrogen bonds occur when the pK values of the atoms sharing the proton are similar. The overall distance is 2.5 A or less, the deuterium fractionation factor is less than 0.5, the proton NMR chemical shift can approach 20 ppm, and deuterium or tritium substitution causes an up-field change...

journal_title：Archives of biochemistry and biophysics

authors： Cleland WW

更新日期：2000-10-01 00:00:00

abstract：:Mutagenic substitution of the invariant D42 and D169 residues in phosphoribulokinase (PRK) with amino acids that contain neutral side chains (e.g., alanine or asparagine) results in large decreases in catalytic efficiency (10(5)- and 10(4)-fold for replacement of D42 and D169, respectively). To further evaluate the im...

journal_title：Archives of biochemistry and biophysics

authors： Runquist JA,Miziorko HM

更新日期：2002-09-15 00:00:00

abstract：:The role of fatty acid metabolism in chemical-dependent cell injury is poorly understood. Addition of L-carnitine to the incubation medium of cultured hepatocytes delayed cell killing initiated by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Protection by L-carnitine was stereospecific and observed as late as ...

journal_title：Archives of biochemistry and biophysics

authors： Snyder JW,Kyle ME,Ferraro TN

更新日期：1990-01-01 00:00:00

abstract：:The interaction of reducing sugars with proteins leads to the formation of advanced glycation end products (AGE) and reactive oxidative species (ROS). ROS peroxidise free or membrane included unsaturated fatty acids, leading to generate reactive aldehydes as advanced lipid peroxidation end products (ALE). Aldehydes fr...

journal_title：Archives of biochemistry and biophysics

authors： Nooshi-Nedamani S,Habibi-Rezaei M,Farzadfard A,Moosavi-Movahedi AA

更新日期：2019-06-15 00:00:00

abstract：:Dipeptidyl-peptidase IV (EC 3.4.14.5) also known as CD26 is a membrane-bound serine peptidase which cleaves N-terminal dipeptides from a peptide chain containing a proline residue in the penultimate position. The enzyme is believed to play an important role in neuropeptide metabolism and T-cell activation. A series of...

journal_title：Archives of biochemistry and biophysics

authors： Li J,Wilk E,Wilk S

更新日期：1995-10-20 00:00:00

abstract：:The electron paramagnetic (EPR) properties of a transient species detected during a cytochrome P450 (P450)-mediated peroxidative reaction have been compared with those of peroxidases Compound I and model metalloporphyrins. The reaction which was studied with cholesterol-specific P450scc and (20R)-20-hydroperoxycholest...

journal_title：Archives of biochemistry and biophysics

authors： Larroque C,Lange R,Maurin L,Bienvenue A,van Lier JE

更新日期：1990-10-01 00:00:00

abstract：:Vanadate V(V) markedly stimulated the oxidation of NADPH by GSSG reductase and this oxidation was accompanied by the consumption of O2 and the accumulation of H2O2. Superoxide dismutases completely eliminated this effect of V(V), whereas catalase was without effect, as was exogenous H2O2 added to 0.1 mM. These effects...

journal_title：Archives of biochemistry and biophysics

authors： Liochev SI,Fridovich I

更新日期：1992-05-01 00:00:00

abstract：:Binding of ligands to the catalytic center of mammalian triosephosphate isomerase (TPI) induces a conformational change(s) that enhances the specific deamidation of Asn71 at the subunit interface. Deamidation initiates dissociation and degradation of the enzyme in vivo and in vitro. We have utilized circular dichroism...

journal_title：Archives of biochemistry and biophysics

authors： Sun AQ,Yüksel KU,Rao GS,Gracy RW

更新日期：1992-06-01 00:00:00

abstract：:Treatment of human pulmonary artery smooth muscle cells (HPASMCs) with the thromboxane A2 receptor antagonist, SQ29548 inhibited U46619 stimulation of phospholipase D (PLD) and NADPH oxidase activities in the cell membrane. Pretreatment with apocynin inhibited U46619 induced increase in NADPH oxidase activity. The cel...

journal_title：Archives of biochemistry and biophysics

authors： Chakraborti S,Sarkar J,Chowdhury A,Chakraborti T

更新日期：2017-11-01 00:00:00

abstract：:The diagnostic substrate tetramethylcyclopropane (TMCP) has been reexamined as a substrate with three drug- and xenobiotic-metabolizing cytochrome P450 enzymes, human CYP2E1, CYP3A4 and rat CYP2B1. The major hydroxylation product in all cases was the unrearranged primary alcohol along with smaller amounts of a rearran...

journal_title：Archives of biochemistry and biophysics

authors： Cooper HL,Groves JT

更新日期：2011-03-01 00:00:00

abstract：:Recently, we characterized a surface antigen (Z-1) of guinea pig macrophages by monoclonal anti-Z-1 antibody. The Z-1 antigen consists of two different polypeptide chains; alpha (140 kDa) and beta (95 kDa). This antigen is closely correlated with the phagocytic activity of the cells for zymosan and presumably function...

journal_title：Archives of biochemistry and biophysics

authors： Hazeki K,Tamoto K,Tada M,Mori Y

更新日期：1989-05-01 00:00:00

abstract：:We have examined the intrinsic fluorescence properties of a highly purified chloroplast H(+)-ATPase (CF0F1) preparation [R. D. Kirch and P. Graber (1992) Acta Physiol. Scand. 746, 9-12). Unlike the catalytic CF1 portion alone, CF0F1 fluorescence was dominated by tryptophan fluorescence both at 277-nm excitation, favor...

journal_title：Archives of biochemistry and biophysics

authors： Kirch RD,Colvert KK,Richter ML,Gräber P

更新日期：1995-02-20 00:00:00

abstract：:Mammalian mitochondria are sensitive targets of the cytotoxic effects of superoxide (O.2-) and nitric oxide (.NO). In turn, when superoxide and nitric oxide are simultaneously produced, they rapidly react with each other yielding the highly oxidizing peroxynitrite anion (ONOO-) which may be also toxic to mammalian mit...

journal_title：Archives of biochemistry and biophysics

authors： Radi R,Rodriguez M,Castro L,Telleri R

更新日期：1994-01-01 00:00:00

abstract：:DNA of livers or kidneys of rodents contain endogenously modified nucleotides of as yet unknown structure or function. As part of our examination of the origin of endogenous DNA modifications, we examined polar adducts of hamster liver and kidney DNA by 32P-postlabeling analysis (central and upper cuts) and compared t...

journal_title：Archives of biochemistry and biophysics

authors： Wang MY,Liehr JG

更新日期：1995-01-10 00:00:00

abstract：:The Ca2+-ATPase activity of isolated membranes and purified plasma membrane ATPase from pig brain was measured in the presence of specific inhibitors. The inhibition of the enzymatic activity by vanadate presents a lower affinity in microsomes than in the synaptic plasma membrane vesicles, showing K0.5 of 0.4 and 0.2 ...

journal_title：Archives of biochemistry and biophysics

authors： Salvador JM,Mata AM

更新日期：1998-03-15 00:00:00

abstract：:Valproic acid (dipropylacetic acid), an antiepileptic agent known to be hepatotoxic in some patients, caused inhibition of lactate gluconeogenesis, fatty acid oxidation, and fatty acid synthesis by isolated hepatocytes. The latter process was the most sensitive to valproic acid, 50% inhibition occurring at ca. 125 mic...

journal_title：Archives of biochemistry and biophysics

authors： Becker CM,Harris RA

更新日期：1983-06-01 00:00:00

abstract：:Venom from the common tiger snake, Notechis scutatus scutatus, contains several toxic acidic proteins which promote hypotension and hemorrhage in mice. One of these toxins, HTe, has a phospholipase A2 (PLA2) amino acid sequence. It contains 125 amino acids rather than the 119/120 found in other N. s. scutatus PLA2s, b...

journal_title：Archives of biochemistry and biophysics

authors： Francis B,Coffield JA,Simpson LL,Kaiser II

更新日期：1995-04-20 00:00:00

abstract：:Horseradish peroxidase (EC 1.11.1.7) is shown to catalyze the aerobic oxidation of lysozyme, bovine serum albumin, and protamine adducts with glycolaldehyde at physiological pH. This reaction is accompanied by light emission, which is attributed to the generation of triplet species. The intensity of chemiluminescence ...

journal_title：Archives of biochemistry and biophysics

authors： de Medeiros MH,Bechara EJ

更新日期：1986-07-01 00:00:00

abstract：:Phosphoenolpyruvate carboxylase from maize leaves was inactivated by pyridoxal 5'-phosphate in the dark and in the light. A two-step reversible mechanism is proposed for inactivation in the dark, which involves the formation of a noncovalent complex prior to a Schiff base with amino groups of the enzyme. Spectral anal...

journal_title：Archives of biochemistry and biophysics

authors： Podesta FE,Iglesias AA,Andreo CS

更新日期：1986-05-01 00:00:00

abstract：:In early starvation tissue protein degradation increases, however in later starvation proteolysis declines so as to pace gradual atrophy during synthetic failure. Secondary decline of proteolytic pathways under progressive nutritional desperation is unexplained. After several days of starvation tissue GSH is partly de...

journal_title：Archives of biochemistry and biophysics

authors： Lockwood TD

更新日期：2003-09-15 00:00:00