UV light, beta-carotene and human skin--beneficial and potentially harmful effects.

abstract：:Uridine phosphorylase is one of the critical enzymes in the pyrimidine salvage pathway. Cells regenerate uridine for nucleotide metabolism by incorporating uracil with ribose-1-phosphate with this enzyme. Recent studies indicate that Escherichia coli uridine phosphorylase is destabilized in the presence of ATP. Howeve...

journal_title：Archives of biochemistry and biophysics

authors： Liu YK,Lin TH,Liu PF

更新日期：2017-11-15 00:00:00

abstract：:Three major pathways mediate amino acid transport into mammalian cells: the A-system and the ASC-system, which require a sodium gradient across the plasma membrane, and the L-system, which has no requirement for a sodium gradient. We have found that the lymphocytes from patients with B-cell chronic lymphocytic leukemi...

journal_title：Archives of biochemistry and biophysics

authors： Segel GB,Tometsko AM,Lichtman MA

更新日期：1985-11-01 00:00:00

abstract：:Sendai virus envelopes have been a useful tool in studying the mechanism of membrane-membrane fusion and have served as a vehicle for introducing foreign molecules (e.g., membrane proteins) into recipient cells. Reconstituted Sendai virus envelopes are routinely obtained following solubilization of virus particles wit...

journal_title：Archives of biochemistry and biophysics

authors： Ran S,Nussbaum O,Loyter A,Marikovsky Y,Rivnay B

更新日期：1988-03-01 00:00:00

abstract：:Several important human inherited neurodegenerative diseases are caused by "polyQ expansions", which are aberrant long repeats of glutamine residues in proteins. PolyQ binding peptide 1 (QBP1), whose minimal active core sequence is Trp-Lys-Trp-Trp-Pro-Gly-Ile-Phe, binds to expanded polyQs and blocks their β-structure ...

journal_title：Archives of biochemistry and biophysics

authors： Ramos-Martín F,Hervás R,Carrión-Vázquez M,Laurents DV

更新日期：2014-09-15 00:00:00

abstract：:Restriction fragments isolated from a 17-kb rat genomic DNA clone containing the gene for apolipoprotein (apo) E were radiolabeled and used to screen a rat liver cDNA library. A cDNA clone hybridizing to a 6-kb genomic DNA fragment was isolated and the nucleotide sequence of the cDNA insert determined. The sequence wa...

journal_title：Archives of biochemistry and biophysics

authors： Shen P,Howlett GJ

更新日期：1992-09-01 00:00:00

abstract：:Biosynthesis of thyroxine in the thyroid gland involves a reaction between two diiodotyrosyl residues within the same molecule of thyroglobulin, a large, thyroid-specific glycoprotein. This reaction, generally referred to as the coupling reaction, is catalyzed in the thyroid by the heme-containing glycoprotein enzyme,...

journal_title：Archives of biochemistry and biophysics

authors： Taurog A,Dorris M,Doerge DR

更新日期：1994-11-15 00:00:00

abstract：:The activity of acetyl-CoA carboxylase (ACC), the rate-limiting enzyme of fatty acid biosynthesis, can be regulated by both adenine and guanine nucleotides in vitro. We have employed two inhibitors of IMP dehydrogenase, ribavarin and tiazofurin, to investigate a possible role for intracellular nucleotides in ACC regul...

journal_title：Archives of biochemistry and biophysics

authors： Witters LA,Mendel DB,Colliton JW

更新日期：1987-01-01 00:00:00

abstract：:Pretreatment of rabbits with imidazole resulted in a twofold increase in hepatic microsomal cytochrome P-450 content, with the apparent induction of two or more distinct forms of the cytochrome [K. K. Hajek and R. F. Novak (1982) Biochem. Biophys. Res. Commun. 108, 664-672]. The metabolic properties of imidazole-induc...

journal_title：Archives of biochemistry and biophysics

authors： Kaul KL,Novak RF

更新日期：1984-12-01 00:00:00

abstract：:To investigate whether several food-derived heterocyclic amines are activated to genotoxic products in human fetal livers, cell lines stably expressing CYP3A7, a human fetus-specific form of cytochrome P450, NADPH-cytochrome P450 reductase, and human monomorphic or polymorphic N-acetyltransferase (NAT1 or NAT2) were e...

journal_title：Archives of biochemistry and biophysics

authors： Hashimoto H,Yanagawa Y,Sawada M,Itoh S,Deguchi T,Kamataki T

更新日期：1995-07-10 00:00:00

abstract：:The lysosomal cysteine proteinases, cathepsins B, H, and L, were all shown to bind to alpha 2-macroglobulin. The bound enzymes remained active against low-molecular-weight synthetic substrates and bound the active-site-directed inhibitor, benzyloxycarbonyl-125I-Tyr-Ala-diazomethane. Binding of the radiolabeled inhibit...

journal_title：Archives of biochemistry and biophysics

authors： Mason RW

更新日期：1989-09-01 00:00:00

abstract：:Peribacteroid membranes can be isolated in essentially pure form from 20-day lupin root nodules by osmotic shock of the purified membrane enclosed bacteroids. The ATPase (EC 3.6.1.3) associated with this membrane has an acid pH optimum (5.25) and is specific for ATP (Mg-ATP Km = 0.16 mM). The enzyme activity requires ...

journal_title：Archives of biochemistry and biophysics

authors： Domigan NM,Farnden KJ,Robertson JG,Monk BC

更新日期：1988-08-01 00:00:00

abstract：:While molecular adaptation to high temperature has been extensively studied, the effect of hydrostatic pressure on protein structure and enzymatic activity is still poorly understood. We have studied the influence of pressure on both the quaternary structure and enzymatic activity of the dodecameric TET3 peptidase fro...

journal_title：Archives of biochemistry and biophysics

authors： Rosenbaum E,Gabel F,Durá MA,Finet S,Cléry-Barraud C,Masson P,Franzetti B

更新日期：2012-01-15 00:00:00

abstract：:Nitroxides stable radicals are unreactive toward most diamagnetic molecules, but readily undergo one-electron redox reactions with paramagnetic species such as free radicals and transition metals, thus serving as cell-permeable antioxidants. The cytotoxicity of juglone (5-hydroxy-1,4-naphthoquinone), like that of othe...

journal_title：Archives of biochemistry and biophysics

authors： Zhang R,Hirsch O,Mohsen M,Samuni A

更新日期：1994-08-01 00:00:00

abstract：:Secreted frizzled-related protein (sFRP)-1 is a Wnt antagonist that inhibits breast carcinoma cell motility, whereas the secreted glycoprotein thrombospondin-1 stimulates adhesion and motility of the same cells. We examined whether thrombospondin-1 and sFRP-1 interact directly or indirectly to modulate cell behavior. ...

journal_title：Archives of biochemistry and biophysics

authors： Martin-Manso G,Calzada MJ,Chuman Y,Sipes JM,Xavier CP,Wolf V,Kuznetsova SA,Rubin JS,Roberts DD

更新日期：2011-05-15 00:00:00

abstract：:The possibility of a cooperative interaction between fat-soluble antioxidants is examined in a membrane model. A combination of beta-carotene and alpha-tocopherol results in an inhibition of lipid peroxidation significantly greater than the sum of the individual inhibitions. Our data provide the first evidence that be...

journal_title：Archives of biochemistry and biophysics

authors： Palozza P,Krinsky NI

更新日期：1992-08-15 00:00:00

abstract：:Adenylate kinase (Adk) that catalyses the synthesis of ADP from ATP and AMP has also been shown to perform an ATP dependent phosphorylation of ribo- and deoxynucleoside diphosphates to their corresponding nucleoside triphosphate; ATP+(d)NDP<-->ADP+(d)NTP. This reaction, suggested to occur by the transfer of the gamma-...

journal_title：Archives of biochemistry and biophysics

authors： Willemoës M,Kilstrup M

更新日期：2005-12-15 00:00:00

abstract：:Human CXCR4 was expressed in Sf9 insect cells using the Bac-to-Bac baculovirus expression system. The recombinant receptor exhibited ligand binding activities with a K(d) value (3.3 nM) comparable to that of the native receptor. The role of four conserved cysteinyl residues was explored by site-directed mutagenesis. E...

journal_title：Archives of biochemistry and biophysics

authors： Zhou H,Tai HH

更新日期：2000-01-01 00:00:00

abstract：:The ferroxidase Fet3 and the permease Ftr1 constitute a well-conserved high-affinity iron uptake system in yeast. We have investigated the mechanism of transcriptional regulation of Fet3 in the methylotrophic yeast Pichia pastoris. Isolation and functional analysis of the Fet3 promoter indicate that a GATA sequence el...

journal_title：Archives of biochemistry and biophysics

authors： Miele R,Barra D,Bonaccorsi di Patti MC

更新日期：2007-09-01 00:00:00

abstract：:Studies in eucaryotic cells (mainly animals and yeast) indicate that at least two DNA polymerases are involved in DNA replication at the level of the replication fork: DNA polymerase alpha, which is associated with DNA primase, is involved in the replication of the lagging strand; DNA polymerase delta, associated with...

journal_title：Archives of biochemistry and biophysics

authors： Richard MC,Litvak S,Castroviejo M

更新日期：1991-05-15 00:00:00

abstract：:Aldehyde oxidase (EC 1.2.3.1) plays a role in the oxidation of aromatic heterocyclic compounds ingested by some higher vertebrates. To better understand this function, the specificity of the rabbit liver enzyme toward purines and their analogs was quantitatively studied. The chemical nature of the 6-substituent of pur...

journal_title：Archives of biochemistry and biophysics

authors： Hall WW,Krenitsky TA

更新日期：1986-11-15 00:00:00

abstract：:Flavopiridol has been shown to induce cell cycle arrest and apoptosis in various tumor cells in vitro and in vivo. Using immobilized flavopiridol, we identified glycogen phosphorylases (GP) from liver and brain as flavopiridol binding proteins from HeLa cell extract. Purified rabbit muscle GP also bound to the flavopi...

journal_title：Archives of biochemistry and biophysics

authors： Kaiser A,Nishi K,Gorin FA,Walsh DA,Bradbury EM,Schnier JB

更新日期：2001-02-15 00:00:00

abstract：:The oxidation of the heme iron of metmyoglobin by H2O2 yields an oxo ferryl complex (FeIV = O), similar to Compound II of peroxidases, as well as a protein radical; this high oxidation state of myoglobin is known as ferrylmyoglobin. The interaction of Trolox, a water-soluble vitamin E analog, with ferrylmyoglobin enta...

journal_title：Archives of biochemistry and biophysics

authors： Giulivi C,Romero FJ,Cadenas E

更新日期：1992-12-01 00:00:00

abstract：:We propose that adverse effects of the antibiotic metronidazole may be due, wholly or in part, to its conversion to a thiamine analog and consequent vitamin B1 antagonism. Consistent with this hypothesis, the drug is accepted as a substrate for the thiaminase (EC 2.5.1.2) elaborated as an exoenzyme by the human gut fl...

journal_title：Archives of biochemistry and biophysics

authors： Alston TA,Abeles RH

更新日期：1987-09-01 00:00:00

abstract：:The ability of nine phospholipids to alter the activity of low-Km cyclic AMP phosphodiesterase was examined in microsomal fractions of rat adipocytes. The enzyme was activated by phosphatidylserine (21% at 300 microM) and phosphatidylglycerol (36% at 300 microM). The activation was concentration dependent over the ran...

journal_title：Archives of biochemistry and biophysics

authors： Macaulay SL,Kiechle FL,Jarett L

更新日期：1983-08-01 00:00:00

abstract：:A kinetic study of acetaminophen oxidation by tyrosinase in the presence of a physiological nucleophilic agent such as the amino acid L-proline is performed in the present paper. The o-quinone product of the catalytic activity, 4-acetamido-o-benzoquinone, becomes unstable through the chemical addition of L-proline, in...

journal_title：Archives of biochemistry and biophysics

authors： Valero E,Varón R,García-Carmona F

更新日期：2003-08-15 00:00:00

abstract：:Primary cultures of rainbow trout hepatocytes were used to study the expression of CYP1A1 mRNA, its protein product (P4501A1), and catalytic activities (7-ethoxy-resorufin-O-deethylase, EROD) during a 96-h period after exposure of cells to beta-naphthoflavone (BNF) or 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Hepato...

journal_title：Archives of biochemistry and biophysics

authors： Pesonen M,Goksøyr A,Andersson T

更新日期：1992-01-01 00:00:00

abstract：:Aryl hydrocarbon hydroxylase activity is most closely associated with cytochrome P-450c in the rat and cytochrome P1-450 in the mouse. The sequence for the orthologous human gene coding for this enzymatic activity has been determined from several sources: cytochrome P-450c isolated from human embryonic DNA [K. Kawajir...

journal_title：Archives of biochemistry and biophysics

authors： Iversen PL,Heiger WJ,Bresnick E,Hines RN

更新日期：1987-07-01 00:00:00

abstract：:Both CYP158A1 and CYP158A2 are able to catalyze an oxidative C-C coupling reaction producing biflaviolin or triflaviolin in Streptomyces coelicolor A3(2). The substrate-bound crystal structures of CYP158A2 and CYP158A1 reveal that the side chain of Ile87 in CYP158A2 points to the active site contacting the distal flav...

journal_title：Archives of biochemistry and biophysics

authors： Zhao B,Bellamine A,Lei L,Waterman MR

更新日期：2012-02-15 00:00:00

abstract：:A 42-kDa plant outer mitochondrial membrane protein, MOM42, has been identified as an essential component of the plant mitochondrial precursor protein translocation apparatus. Immunological cross-reactivity has been detected between antibodies raised against both Neurospora and yeast mitochondrial outer membrane prote...

journal_title：Archives of biochemistry and biophysics

authors： Perryman RA,Mooney B,Harmey MA

更新日期：1995-02-01 00:00:00

abstract：:Staphylococcus aureus is associated with several clinically significant infections among humans and infections associated with antibiotic-resistant strains are growing in frequency. Antivirulence strategies shift the target of drugs from bacterial growth to the infection process resulting to milder evolutionary pressu...

journal_title：Archives of biochemistry and biophysics

authors： Cueno ME,Imai K

更新日期：2018-05-01 00:00:00