Identification of a 42-kDa plant mitochondrial outer membrane protein, MOM42, involved in the import of precursor proteins into plant mitochondria.

abstract：:Rats fed an ethanol-containing diet for 4 weeks showed a 3- to 5-fold increase over isocalorically pair-fed controls with respect to cytosolic NAD(P)H-quinone oxidoreductase (NQOR) (E.C.1.6.99.2) with both menadione and dichlorophenol-indophenol as substrates. Rates of NAD(P)H-dependent p-nitrosophenol (pNSP) reductio...

journal_title：Archives of biochemistry and biophysics

authors： Hajos AK,Winston GW

更新日期：1992-06-01 00:00:00

abstract：:The pH gradient, delta pH, present across the inner mitochondrial membrane in isolated rat hepatocytes was continuously monitored with a novel spectroscopic technique that utilizes the weak acid fluorescein. Unlike most cytosolic pH indicators, such as 2',7'-bis(carboxyethyl)-5,(6)-carboxyfluorescein (BCECF), fluoresc...

journal_title：Archives of biochemistry and biophysics

authors： Thomas PJ,Gaspers LD,Pharr C,Thomas JA

更新日期：1991-07-01 00:00:00

abstract：:Rabbit antibodies raised against the hydrophilic part of microsomal NADPH-cytochrome P450 oxidoreductase (denoted fpT) demonstrated a marked ability to inhibit NADPH-sterol Delta7-reductase activity. In addition, trypsin and proteinase K treatment of microsomes removed almost all microsomal electron transfer constitue...

journal_title：Archives of biochemistry and biophysics

authors： Nishino H,Ishibashi T

更新日期：2000-02-15 00:00:00

abstract：:The cellular environment is crowded with different kinds of molecules with varying sizes, shapes and compositions. Most of the experiments studying the nature and behaviour of a protein have been done on the isolated protein in dilute buffer solutions which actually do not imitate the in vivo situation. To understand ...

journal_title：Archives of biochemistry and biophysics

authors： Shahid S,Ahmad F,Hassan MI,Islam A

更新日期：2015-10-15 00:00:00

abstract：:The effects of fluorescein isothiocyanate II (FITC) on the actions of insulin in rat adipocytes were studied. When adipocytes were incubated with FITC at pH 7.4 (2 mM agent, 8 min), the cells were completely deprived of their specific insulin-binding activity and rendered unresponsive to the hormone. The effect of FIT...

journal_title：Archives of biochemistry and biophysics

authors： Goto Y,Sumida Y,Flanagan JE,Robinson FW,Simpson IA,Cushman SW,Kono T

更新日期：1992-03-01 00:00:00

abstract：:High-Mr and monomeric adenosine deaminase were injected intravenously into the rabbit. The rates of clearance and sites of uptake of the enzymes were compared. Calf intestinal mucosa served as the source of monomeric adenosine deaminase. High-Mr enzymes were assembled in vitro from the calf enzyme and adenosine deamin...

journal_title：Archives of biochemistry and biophysics

authors： Schrader WP,Harder CM,Schrader DK,West CA

更新日期：1984-04-01 00:00:00

abstract：:The role of malnutrition in the development of cachexia in rats bearing the Yoshida ascites hepatoma AH-130 was investigated by comparing the changes in tissue protein turnover in these animals with those observed in pair-fed controls. The tumor elicited in rats an early and conspicuous loss of body weight and tissue ...

journal_title：Archives of biochemistry and biophysics

authors： Tessitore L,Costelli P,Bonetti G,Baccino FM

更新日期：1993-10-01 00:00:00

abstract：:African trypanosomes of the Trypanosoma brucei group are agents of disease in man and animals. They present unique biochemical characteristics such as the need for preformed purines and have extensive salvage mechanisms for nucleoside recovery. In this regard we have shown that trypanosomes have a dedicated transporte...

journal_title：Archives of biochemistry and biophysics

authors： Goldberg B,Rattendi D,Lloyd D,Yarlett N,Bacchi CJ

更新日期：1999-04-01 00:00:00

abstract：:The important characteristic of novel Mimosa pudica tubulin is its ability to bind colchicine only when dithiothreitol is included in the isolation buffer, indicating the involvement of sulfhydryl groups in colchicine binding. Modification of sulfhydryl groups by a sulfhydryl modifying agent also affects the normal as...

journal_title：Archives of biochemistry and biophysics

authors： Roychaudhuri A,Biswas S

更新日期：1992-05-01 00:00:00

abstract：:A new enzyme homologous to phosphotriesterase was identified from the bacterium Geobacillus stearothermophilus (GsP). This enzyme belongs to the amidohydrolase family and possesses the ability to hydrolyze both lactone and organophosphate (OP) compounds, making it a phosphotriesterase-like lactonase (PLL). GsP possess...

journal_title：Archives of biochemistry and biophysics

authors： Hawwa R,Aikens J,Turner RJ,Santarsiero BD,Mesecar AD

更新日期：2009-08-15 00:00:00

abstract：:Atroxase, isolated from the venom of Crotalus atrox (western diamondback rattlesnake), is a nonhemorrhagic protease which has fibrinolytic activity in vivo. The primary structure of atroxase was deduced from the cDNA encoding the atroxase protein. The venom glands of Crotalus atrox were used to prepare a cDNA library....

journal_title：Archives of biochemistry and biophysics

authors： Baker BJ,Wongvibulsin S,Nyborg J,Tu AT

更新日期：1995-03-10 00:00:00

abstract：:This report describes the post-translational modifications of recombinant human differentiation-stimulating factor, a 180-residue glycoprotein that is secreted from transfected Chinese hamster ovary cells. Peptide peptides containing six potential N-glycosylation sites were analyzed to determine that Asn residues 9, 3...

journal_title：Archives of biochemistry and biophysics

authors： Schmelzer CH,Harris RJ,Butler D,Yedinak CM,Wagner KL,Burton LE

更新日期：1993-05-01 00:00:00

abstract：:Because of the severe limitations on growing large quantities of Drosophila affinidisjuncta in the laboratory, direct purification of alcohol dehydrogenase (ADH) from this species has proven impossible. As an alternative source of this enzyme, a cDNA encoding functional ADH was isolated from a newly constructed cDNA l...

journal_title：Archives of biochemistry and biophysics

authors： Green MM,Fang XM,Churchill P,Brennan MD

更新日期：1989-09-01 00:00:00

abstract：:We have shown that superoxide (O2.-) is produced during the oxidation of veratryl alcohol by lignin peroxidase (LiP) by the reaction of the veratryl alcohol cation radical with hydrogen peroxide (D. B. Barr, M. M. Shah, and S. D. Aust, 1993, J. Biol. Chem. 268, 241-244). Compound III, an inactive form of peroxidases c...

journal_title：Archives of biochemistry and biophysics

authors： Barr DP,Aust SD

更新日期：1994-06-01 00:00:00

abstract：:2-Isopropenyl-2-methyladamantane (2-PMADA) and 3-isopropenyl-3-methyldiamantane (3-PMDIA) showed potent and selective inhibition of cytochrome P450 (CYP) 2B6-mediated reactions with K(i) values of 5.27 and 2.17 microM, respectively. No effect on activities of other human CYP was found even at concentrations 100-fold h...

journal_title：Archives of biochemistry and biophysics

authors： Stiborová M,Borek-Dohalská L,Hodek P,Mráz J,Frei E

更新日期：2002-07-01 00:00:00

abstract：:The properties of porcine platelet acyltransferases which catalyze the incorporation of unsaturated fatty acids into the 2 positions of phospholipids were compared with those of porcine liver microsomes and rat liver microsomes. There were significant differences in the relative rates of incorporation of acyl groups i...

journal_title：Archives of biochemistry and biophysics

authors： Inoue M,Murase S,Okuyama H

更新日期：1984-05-15 00:00:00

abstract：:In vitro phosphorylation of purified spectrin dimer was studied in the presence of Ca2+-calmodulin (CaM). CaM inhibited autophosphorylation of the beta subunit of spectrin. The inhibitory effect (65% at a 32-fold molar excess) appeared to be due to a weak interaction of CaM with spectrin. CaM was similarly effective i...

journal_title：Archives of biochemistry and biophysics

authors： Maretzki D,Lutz HU

更新日期：1986-04-01 00:00:00

abstract：:Human leukemic T-lymphocytes undergo extensive and rapid apoptosis in the presence of L1AD3, a small cyclic peptide derivative of cobra cardiotoxin. The first step in this process involves its binding to membranes of susceptible cells. By the use of a biotin "handle" synthetically incorporated at the N-terminus of L1A...

journal_title：Archives of biochemistry and biophysics

authors： Smith CA,Hinman CL

更新日期：2004-12-01 00:00:00

abstract：:Protein tyrosine kinase Yes is a cellular homolog of v-Yes, the oncogenic protein product of avian sarcoma virus Y73. Yes is a member of the Src family and its activation has been associated with several types of human cancer. Human Yes has not been previously characterized enzymatically. To carry out biochemical char...

journal_title：Archives of biochemistry and biophysics

authors： Sun G,Budde RJ

更新日期：1997-09-01 00:00:00

abstract：:Vacuolar H(+)-translocating inorganic pyrophosphatase (V-PPase; EC 3.6.1.1) is a homodimeric proton-translocase; it contains a single type of polypeptide of approximately 81kDa. A line of evidence demonstrated that the carboxyl terminus of V-PPase is relatively conserved in various plant V-PPases and presumably locate...

journal_title：Archives of biochemistry and biophysics

authors： Lin HH,Pan YJ,Hsu SH,Van RC,Hsiao YY,Chen JH,Pan RL

更新日期：2005-10-15 00:00:00

abstract：:Peroxiredoxin 6 (Prdx6) differs from other mammalian peroxiredoxins both in its ability to reduce phospholipid hydroperoxides at neutral pH and in having phospholipase A(2) (PLA(2)) activity that is maximal at acidic pH. We previously showed an active site C47 for peroxidase activity and a catalytic triad S32-H26-D140...

journal_title：Archives of biochemistry and biophysics

authors： Manevich Y,Shuvaeva T,Dodia C,Kazi A,Feinstein SI,Fisher AB

更新日期：2009-05-15 00:00:00

abstract：:The enzyme catalase catalyzes the breakdown of hydrogen peroxide into oxygen and water. It is the main regulator of hydrogen peroxide metabolism. Hydrogen peroxide is a highly reactive small molecule formed as a natural byproducts of energy metabolism. Excessive concentrations may cause significant damages to protein,...

journal_title：Archives of biochemistry and biophysics

authors： Góth L,Nagy T

更新日期：2012-09-15 00:00:00

abstract：:Chronic kidney disease (CKD) is an increasingly recognized burden for patients and health care systems with high (and growing) global incidence and prevalence, significant mortality, and disproportionately high treatment costs. Yet, the available diagnostic tools are either impractical in clinical routine or have seri...

journal_title：Archives of biochemistry and biophysics

authors： Breit M,Weinberger KM

更新日期：2016-01-01 00:00:00

abstract：:Cultured rat hepatocytes were treated with potassium cyanide, an inhibitor of cytochrome oxidase; valinomycin, a K+ ionophore; carbonyl cyanide m-chlorophenylhydrazone (CCCP), a protonophore; and the ATP synthetase inhibitor oligomycin. The effect of these agents on the viability of the cells was related to changes in...

journal_title：Archives of biochemistry and biophysics

authors： Masaki N,Thomas AP,Hoek JB,Farber JL

更新日期：1989-07-01 00:00:00

abstract：:Ringer's lactate solution irradiated by non-thermal plasma, comprised of radicals, electrons, and ions, is defined as plasma-activated lactate (PAL). PAL exhibited antitumor effects in glioblastoma U251SP cells, which we termed PAL-specific regulated cell death. In contrast to the oxidative stress condition typical of...

journal_title：Archives of biochemistry and biophysics

authors： Ishikawa K,Hosoi Y,Tanaka H,Jiang L,Toyokuni S,Nakamura K,Kajiyama H,Kikkawa F,Mizuno M,Hori M

更新日期：2020-07-30 00:00:00

abstract：:Electron spin resonance (ESR) spectroscopy and spin label techniques have been used to study the effects of fumonisin B1 (FB1) and hydrolyzed fumonisin backbone (AP1) on the structural and dynamic properties of phosphatidylcholine membranes at the molecular level. Multilamellar liposomes consisting of dimyristoylphosp...

journal_title：Archives of biochemistry and biophysics

authors： Yin JJ,Smith MJ,Eppley RM,Troy AL,Page SW,Sphon JA

更新日期：1996-11-01 00:00:00

abstract：:Oxidative stress is a mechanism with a central role in the pathogenesis of atherosclerosis, cancer, and other chronic diseases. It also plays a major role in the aging process. Ischemic heart disease is perhaps the human condition in which the role of oxidative stress has been investigated in more detail: reactive oxy...

journal_title：Archives of biochemistry and biophysics

authors： Ceconi C,Boraso A,Cargnoni A,Ferrari R

更新日期：2003-12-15 00:00:00

abstract：:The stability of the Glossoscolex paulistus hemoglobin (HbGp), in two iron oxidation states (and three forms), as monitored by optical absorption, fluorescence emission and circular dichroism (CD) spectroscopies, in the presence of the chaotropic agent urea, is studied. HbGp oligomeric dissociation, denaturation and i...

journal_title：Archives of biochemistry and biophysics

authors： Carvalho FA,Santiago PS,Tabak M

更新日期：2012-03-01 00:00:00

abstract：:Heparin is thought to regulate the rate of mammalian blood clotting by enhancing the activity of antithrombin, an inhibitor of coagulation enzymes. The present study establishes that this same inhibitor is present in the blood plasma of each of the terrestrial vertebrate groups including mammals, birds, reptiles, and ...

journal_title：Archives of biochemistry and biophysics

authors： Jordan RE

更新日期：1983-12-01 00:00:00

abstract：:The reaction center of photosystem 2 has been highly purified from digitonin-solubilized thylakoid membranes of the thermophilic cyanobacterium Synechococcus sp. by means of sucrose density gradient centrifugation and electrophoresis on polyacrylamide gels containing digitonin. Sodium dodecyl sulfate-polyacrylamide ge...

journal_title：Archives of biochemistry and biophysics

authors： Yamagishi A,Katoh S

更新日期：1983-09-01 00:00:00