Inhibition of taurolithocholate 3-sulfate-induced apoptosis by cyclic AMP in rat hepatocytes involves protein kinase A-dependent and -independent mechanisms.


:The mechanisms underlying the inhibition of bile acid-induced apoptosis by cyclic AMP (cAMP) were studied in 24-h-cultured rat hepatocytes. Taurolithocholate 3-sulfate (TLCS, 100 micromol/l) led to a sustained activation of mitogen activated protein (MAP) kinases (JNK, p38(MAPK), and ERKs), dephosphorylation of protein kinase B (PKB), activation of caspases 3 and 8, and hepatocyte apoptosis. cAMP prevented TLCS-induced apoptosis, shifted the persistent TLCS-induced MAP kinase response to a transient pattern, and prevented PKB dephosphorylation. TLCS-induced CD95 and TRAIL receptor-2 trafficking to the plasma membrane were significantly inhibited. Blockade of protein kinase A (PKA) abolished the inhibitory effect of cAMP on TLCS-induced CD95 membrane targeting, but not TRAIL receptor-2 membrane targeting, PKB and MAP kinase responses. H89, an inhibitor of PKA, had no effect on cAMP-induced inhibition of TLCS-triggered poly(ADP) ribose polymerase (PARP) cleavage and caspase activation, but abolished the cAMP-induced inhibition of TLCS-triggered TUNEL- and Annexin V staining. It is concluded that cAMP inhibits bile acid-induced apoptosis via PKA-dependent and -independent mechanisms.


Arch Biochem Biophys


Graf D,Reinehr R,Kurz AK,Fischer R,Häussinger D




Has Abstract


2003-07-01 00:00:00














  • Insulin increases the synthesis of phospholipid and diacylglycerol and protein kinase C activity in rat hepatocytes.

    abstract::The effects of insulin on phospholipid metabolism and generation of diacylglycerol (DAG) and on activation of protein kinase C in rat hepatocytes were compared to those of vasopressin and angiotension II. Insulin provoked increases in [3H]glycerol labeling of phosphatidic acid (PA), diacylglycerol (DAG), and other gly...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Cooper DR,Hernandez H,Kuo JY,Farese RV

    更新日期:1990-02-01 00:00:00

  • The mechanism of alpha 1-antitrypsin polymerization probed by fluorescence spectroscopy.

    abstract::The polymerization of alpha1-antitrypsin within the hepatic cell leads to alpha1-antitrypsin deficiency. Both the conformational changes and the kinetics of the polymerization process are poorly understood. Here we describe fluorescence experiments investigating the polymerization reaction using the fluorescent probe4...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: James EL,Bottomley SP

    更新日期:1998-08-15 00:00:00

  • Reexamination of the carbohydrate binding stoichiometry of lima bean lectin.

    abstract::The carbohydrate binding stoichiometry of lima bean lectin component III was reexamined using equilibrium dialysis and quantitative affinity chromatography following limited chemical modification. Equilibrium dialysis employing methyl[2-14C]benzamido-2-deoxy-alpha-D-galactopyranoside as ligand demonstrated that the le...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Roberts DD,Goldstein IJ

    更新日期:1984-04-01 00:00:00

  • Purification and characterization of an iron-containing superoxide dismutase from a eucaryote, Ginkgo biloba.

    abstract::A cyanide-insensitive superoxide dismutase was purified to electrophoretic homogeneity from leaves of the eucaryote, Ginkgo biloba L. A molecular mass of 47,000 was determined for the enzyme, which consisted of two subunits of equal size. The enzyme preparation contained two isoenzymes with isoelectric points of 5.25 ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Duke MV,Salin ML

    更新日期:1985-11-15 00:00:00

  • Characterization of purified avian 90,000-Da heat shock protein.

    abstract::The so-called 90,000-Da heat shock protein (hsp90) from chicken liver has been purified and physically characterized in the presence of high levels of the serine phosphatase inhibitor fluoride. The protein is an elongated dimer with a molecular weight of 160,000 and a frictional ratio of 1.6. On two-dimensional electr...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Iannotti AM,Rabideau DA,Dougherty JJ

    更新日期:1988-07-01 00:00:00

  • Toxicity of S-pentachlorobutadienyl-L-cysteine studied with isolated rat renal cortical mitochondria.

    abstract::The subcellular mechanism of alkenyl halide S-conjugate-induced nephrotoxicity was studied in mitochondria isolated from rat kidney cortex in vitro using the cysteine conjugate of hexachloro-1,3-butadiene, i.e., S-pentachlorobutadienyl-L-cysteine (PCBC) as a model substrate. Respiring mitochondria exposed to various c...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Wallin A,Jones TW,Vercesi AE,Cotgreave I,Ormstad K,Orrenius S

    更新日期:1987-11-01 00:00:00

  • Identification of the mannan-binding protein from rat livers as a hepatocyte protein distinct from the mannan receptor on sinusoidal cells.

    abstract::Cellular distribution in rat livers of the mannan-binding protein, a liver lectin specific for mannose and N-acetylglucosamine was investigated. Estimation of mannan binding activity of isolated cells demonstrated that more than 99% of the mannan binding activity recovered from whole livers was derived from hepatocyte...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Mori K,Kawasaki T,Yamashina I

    更新日期:1983-04-15 00:00:00

  • Dinoflagellate luminescence: purification of a NAD(P)H-dependent reductase and of its substrate.

    abstract::The soluble enzymatic luminescent system of the dinoflagellate Pyrocystis lunula (luciferase-luciferin) is coupled with an enzymatic NAD(P)H-dependent reaction. The enzyme is a soluble reductase (Mr 47,000) which catalyzes, in the presence of NAD(P)H, the reduction of a molecule called P630. Reduced P630 has the same ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Fresneau C,Hill M,Lescure N,Arrio B,Dupaix A,Volfin P

    更新日期:1986-12-01 00:00:00

  • Characterization of glycosylphosphatidylinositol (GPI)-anchored NCAM on mouse skeletal muscle cell line C2C12: the structure of the GPI glycan and release during myogenesis.

    abstract::The mouse myoblast cell line C2C12 constitutively expressed 160-kDa transmembrane NCAM isoform and 135-kDa GPI-anchored isoform before differentiation. During differentiation into multinucleated myotubes, the cells newly expressed 150-kDa GPI-anchored isoform and the level of 135-kDa GPI-anchored isoform increased. St...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Mukasa R,Umeda M,Endo T,Kobata A,Inoue K

    更新日期:1995-04-01 00:00:00

  • Characterization of tryptic hydrolysis of alpha-lactalbumin/saponin mixture and structural change of alpha-lactalbumin interacting with soybean saponin.

    abstract::Bovine milk alpha-lactalbumin (alpha-La) was mixed with soybean saponin, and the resulting mixture was hydrolyzed by trypsin. Saponin increased the tryptic-hydrolysis level of alpha-La only at relatively high phosphate buffer concentrations (> or = 0.05 M). T(1) experiments with acetylated soybean saponin demonstrated...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Shimoyamada M,Okada Y,Watanabe K,Yamauchi R

    更新日期:2005-03-15 00:00:00

  • Effects of oxidants on properties of fluorescent calcium indicators.

    abstract::An increasing number of studies use calcium-sensitive fluorescent dyes to address the relationship between elevated levels of intracellular calcium and free-radical-mediated damage in a variety of pathophysiological phenomena. The present study evaluates the effects of reactive oxygen species on the spectral propertie...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Sarvazyan N,Swift L,Martinez-Zaguilan R

    更新日期:1998-02-01 00:00:00

  • Nucleotide sequence encoding the snake venom fibrinolytic enzyme atroxase obtained from a Crotalus atrox venom gland cDNA library.

    abstract::Atroxase, isolated from the venom of Crotalus atrox (western diamondback rattlesnake), is a nonhemorrhagic protease which has fibrinolytic activity in vivo. The primary structure of atroxase was deduced from the cDNA encoding the atroxase protein. The venom glands of Crotalus atrox were used to prepare a cDNA library....

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Baker BJ,Wongvibulsin S,Nyborg J,Tu AT

    更新日期:1995-03-10 00:00:00

  • Cardiotonic actions of quercetin and its metabolite tamarixetin through a digitalis-like enhancement of Ca2+ transients.

    abstract::The plant-derived flavonoid, quercetin (QCT), has many biological actions, including cardioprotective actions, resulting from its antioxidant and anti-inflammatory effects. In this study, effects of QCT and its metabolites on the contraction and Ca2+ transients (CaT) of mouse single cardiomyocytes were simultaneously ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Hayamizu K,Morimoto S,Nonaka M,Hoka S,Sasaguri T

    更新日期:2018-01-01 00:00:00

  • Modulation of acetyl-CoA carboxylase by inhibitors of IMP dehydrogenase: implications for insulin regulation.

    abstract::The activity of acetyl-CoA carboxylase (ACC), the rate-limiting enzyme of fatty acid biosynthesis, can be regulated by both adenine and guanine nucleotides in vitro. We have employed two inhibitors of IMP dehydrogenase, ribavarin and tiazofurin, to investigate a possible role for intracellular nucleotides in ACC regul...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Witters LA,Mendel DB,Colliton JW

    更新日期:1987-01-01 00:00:00

  • Reconstitution of fusogenic Sendai virus envelopes by the use of the detergent chaps.

    abstract::Sendai virus envelopes have been a useful tool in studying the mechanism of membrane-membrane fusion and have served as a vehicle for introducing foreign molecules (e.g., membrane proteins) into recipient cells. Reconstituted Sendai virus envelopes are routinely obtained following solubilization of virus particles wit...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Ran S,Nussbaum O,Loyter A,Marikovsky Y,Rivnay B

    更新日期:1988-03-01 00:00:00

  • Woodchuck VEGF (wVEGF) characteristics: Model for angiogenesis and human hepatocellular carcinoma directed therapies.

    abstract::Vascular endothelial growth factor (VEGF) stimulates angiogenesis. Human hepatocellular carcinoma (HCC) is a VEGF-driven tumor often associated with chronic hepatitis B or C virus infection. The woodchuck is a well-characterized model of hepatitis B virus related HCC and a valuable tool for translational studies of no...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Huang H,Salavaggione O,Rivera L,Mukherjee S,Brekken R,Tennant B,Iyer R,Adjei A

    更新日期:2019-01-01 00:00:00

  • Identification of the active-site serine in human lecithin: cholesterol acyltransferase.

    abstract::Purified human lecithin:cholesterol acyltransferase (LCAT) was covalently labeled by [3H]diisopropylflourophosphate with concomitant loss of enzymatic activity (M. Jauhiainen and P.J. Dolphin (1986) J. Biol. Chem. 261, 7023-7043). Some 60% of the enzyme was labeled in 1 h. Cyanogen bromide (CNBr) cleavage of the label...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Farooqui JZ,Wohl RC,Kézdy FJ,Scanu AM

    更新日期:1988-03-01 00:00:00

  • omega-haloalkyl esters of 5'-adenosine monophosphate as potential active-site-directed reagents for dehydrogenases.

    abstract::A series of esters of adenosine 5'-monophosphate with ethyl, propyl, or hexyl moieties substituted at the omega-position with chlorine or bromine were prepared. The compounds were competitive inhibitors of horse liver alcohol dehydrogenase with respect to coenzyme, NAD+, and had inhibition (dissociation) constants in ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Fries RW,Bohlken DP,Murch BP,Leidal KG,Plapp BV

    更新日期:1983-08-01 00:00:00

  • In vivo reversal of glutathione deficiency and susceptibility to in vivo dexamethasone-induced apoptosis by N-acetylcysteine and L-2-oxothiazolidine-4-carboxylic acid, but not ascorbic acid, in thymocytes from gamma-glutamyltranspeptidase-deficient knocko

    abstract::Cellular glutathione is released during apoptosis and may play a role in the regulation of the mitochondrial permeability transition pore. The question of whether only cytosolic glutathione is important in apoptosis, or whether mitochondrial glutathione also plays a role, was investigated using gamma-glutamyltranspept...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Will Y,Kaetzel RS,Brown MK,Fraley TS,Reed DJ

    更新日期:2002-01-15 00:00:00

  • Minimizing frustration by folding in an aqueous environment.

    abstract::Although life as we know it evolved in an aqueous medium, the properties of water are not completely understood. In this review, we focus on the role of water in guiding protein folding and stability. Specifically, we discuss the mechanisms of protein folding in an aqueous environment, the effects of water on the fold...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Mattos C,Clark AC

    更新日期:2008-01-01 00:00:00

  • Differential binding properties of human pregnancy zone protein- and alpha2-macroglobulin-proteinase complexes to low-density lipoprotein receptor-related protein.

    abstract::Human pregnancy zone protein (PZP) is a major pregnancy-associated plasma protein strongly related to alpha2-macroglobulin (alpha2-M). Both alpha-macroglobulins (alpha-Ms) covalently bind proteinases, which is accompanied by the exposure of carboxy terminal receptor recognition domains important for the rapid clearanc...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Chiabrando GA,Vides MA,Sánchez MC

    更新日期:2002-02-01 00:00:00

  • Difference in polymerization and steady-state dynamics of free and gelsolin-capped filaments formed by alpha- and beta-isoactins.

    abstract::The polymerization of scallop beta-like actin is significantly slower than that of skeletal muscle alpha-actin. To reveal which steps of polymerization contribute to this difference, we estimated the efficiency of nucleation of the two actins, the rates of filament elongation at spontaneous and gelsolin-nucleated poly...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Khaitlina S,Hinssen H

    更新日期:2008-09-15 00:00:00

  • Reoxygenation injury and antioxidant protection: a tale of two paradoxes.

    abstract::Under certain circumstances, added antioxidants can protect tissues against reoxygenation injury after ischemia. Yet reperfusing blood carries many antioxidants with it. The implications of this "antioxidant paradox" are discussed. ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章,评审


    authors: Gutteridge JM,Halliwell B

    更新日期:1990-12-01 00:00:00

  • Role of vascular nitric oxide synthase in endotoxin shock of Propionibacterium acnes-sensitized rats.

    abstract::The sensitivity of animals to endotoxin differs significantly between species. Thus, factors that determine the susceptibility to endotoxin may play important roles in the pathogenesis of septic shock. In order to determine the mechanism responsible for susceptibility to endotoxin, the effect of lipopolysaccharide (LP...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Kida T,Kuroki T,Kobayashi K,Inoue M

    更新日期:1994-07-01 00:00:00

  • Effect of sulfhydryl reagents on nucleoside transport in cultured mammalian cells.

    abstract::Incubation of Novikoff rat hepatoma cells; mouse L929, P388 and L1210 cells; and Chinese hamster ovary cells with sulfhydryl reagents, such as p-hydroxymercuribenzoate or p-hydroxymercuribenzenesulfonate, reduced the zero-trans influx of uridine in a concentration-dependent manner. The sensitivity of uridine transport...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Plagemann PG,Wohlhueter RM

    更新日期:1984-09-01 00:00:00

  • The plasma membrane Ca2+ ATPase of animal cells: structure, function and regulation.

    abstract::Most important processes in cell life are regulated by calcium (Ca2+). A number of mechanisms have thus been developed to maintain the concentration of free Ca2+ inside cells at the level (100-200nM) necessary for the optimal operation of the targets of its regulatory function. The systems that move Ca2+ back and fort...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章,评审


    authors: Di Leva F,Domi T,Fedrizzi L,Lim D,Carafoli E

    更新日期:2008-08-01 00:00:00

  • Nitric oxide inhibition of lipoxygenase-dependent liposome and low-density lipoprotein oxidation: termination of radical chain propagation reactions and formation of nitrogen-containing oxidized lipid derivatives.

    abstract::Lipoxygenase-induced lipid oxidation contributes to plasma lipoprotein oxidation and may be an underlying pathogenic mechanism of atherogenesis. Since inactivation of the vasorelaxant actions of nitric oxide (.NO) plays a critical role in the impaired function of atherosclerotic vessels and because .NO reacts rapidly ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Rubbo H,Parthasarathy S,Barnes S,Kirk M,Kalyanaraman B,Freeman BA

    更新日期:1995-12-01 00:00:00

  • Asparagine catabolism in rat liver mitochondria.

    abstract::A large portion of mitochondrial asparagine (Asn) is degraded by asparagine amino-transferase to produce alpha-ketosuccinamate (alpha KSA), which is then hydrolized by omega-amidase to produce oxaloacetate (OAA) and ammonia. This is in contrast to the catabolism in the cytosol, where the main catabolic route for Asn o...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Moraga-Amador DA,MacPhee-Quiggley KM,Keefer JF,Schuster SM

    更新日期:1989-01-01 00:00:00

  • Protein composition of the atrial muscarinic acetylcholine receptor partially purified by wheat germ agglutinin affinity chromatography.

    abstract::The use of affinity chromatography on wheat germ agglutinin columns to partially purify detergent extracts of muscarinic acetylcholine receptor from porcine atria is described. The procedure results in a 20-fold purification of the protein. The partially purified protein binds [3H]L-QNB (the L isomer of quinuclidinyl ...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Herron GS,Schimerlik MI

    更新日期:1984-05-01 00:00:00

  • Binding of monoclonal antibody to cathepsin M located on the external surface of rabbit lysosomes.

    abstract::A monoclonal antibody raised against rabbit liver cathepsin M binds to intact rabbit liver lysosomes. The binding is specific and is abolished by treating the lysosomes with trypsin, which has previously been shown to digest the membrane-bound cathepsin M [S. Pontremoli, E. Melloni, M. Michetti, F. Salamino, B. Sparat...

    journal_title:Archives of biochemistry and biophysics

    pub_type: 杂志文章


    authors: Pontremoli S,Melloni E,Damiani G,Michetti M,Salamino F,Sparatore B,Horecker BL

    更新日期:1984-08-15 00:00:00