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Small-angle x-ray scattering of high- and low-affinity heparin.

Abstract：

:Solution characterization of heparin with high affinity (HA) and low affinity (LA) for antithrombin III was performed using the methods of small-angle x-ray scattering (SAXS), viscometry, and aqueous gel permeation chromatography (GPC). SAXS provided various topological parameters including the radius of gyration ([S2]1/2), radius of gyration of cross-section ([S2]q1/2), persistence length (a*), contour length (L), and mass parameters, e.g., overall molecular mass (Mr), and mass per unit length (Mq). The molecular weights of HA and LA pig mucosal heparins were found to be 14,900 and 11,500 and the respective radii of gyration were 40.1 and 33.6 A. The persistence lengths of HA and LA were 21.3 and 20.3 A, respectively. These parameters were compared to SAXS data of heparin [S. S. Stivala, M. Herbst, O. Kratky, and I. Pilz (1968) Arch. Biochem. Biophys. 127, 795-802] fractionated according to molecular weight only. It was found that the various experimental values of this heparin lie somewhere in between those of HA and LA heparins. It appears that there are no appreciable differences in the physico-chemical properties, including conformation, among the heparins in H2O at 25 degrees C.

journal_name

Arch Biochem Biophys

journal_title

Archives of biochemistry and biophysics

authors

Khorramian BA,Stivala SS

doi

10.1016/0003-9861(86)90597-7

subject

Has Abstract

pub_date

1986-06-01 00:00:00

pages

384-92

issue

2

eissn

0003-9861

issn

1096-0384

pii

0003-9861(86)90597-7

journal_volume

247

pub_type

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