Abstract：
:Osteocytes sense extracellular mechanical stimuli and transduce them into biochemical signals to regulate bone remodeling. The function is also evidenced in orthodontic tooth movement. But the underlying mechanisms haven't been clarified. Autophagy is an evolutionarily conserved cellular catabolic process which affects cellular secretory capabilities. We hypothesized that mechanical force activated osteocyte autophagy through TFE3-related signaling and further promoted osteocyte-mediated osteoclastogenesis. In the present study, we demonstrated that osteocyte autophagy was activated under mechanical compressive force using murine orthodontic tooth movement model since the number of LC3B-positive osteocytes increased by 3-fold in the compression side. In addition, both in vitro mechanical compression and chemical autophagy agonist increased the secretion of RANKL in osteocytes by 3-fold and 4-fold respectively, which is a crucial cytokine for osteoclastogenesis. Lastly, conditioned medium collected from compressed osteocytes promoted the development of osteoclasts. These results suggest that osteocytes could promote osteoclastogenesis via autophagy-mediated RANKL secretion under mechanical compressive force. Our research might provide evidence for exploring methods to accelerate tooth movement in clinic.
journal_name
Arch Biochem Biophysjournal_title
Archives of biochemistry and biophysicsauthors
Li W,Zhao J,Sun W,Wang H,Pan Y,Wang L,Zhang WBdoi
10.1016/j.abb.2020.108594subject
Has Abstractpub_date
2020-11-15 00:00:00pages
108594eissn
0003-9861issn
1096-0384pii
S0003-9861(20)30603-2journal_volume
694pub_type
杂志文章abstract：:The enhancement of the binding of plasma fibronectin to collagen or gelatin by heparin was previously thought to be due primarily to interaction of heparin with fibronectin. We observed, however, that the elution of purified human plasma fibronectin from heparin-treated gelatin-agarose required the same high urea conc...
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更新日期：1996-08-15 00:00:00
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pub_type： 杂志文章,评审
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pub_type： 杂志文章
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更新日期：2018-08-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2009-03-15 00:00:00
