Triplex forming ability of a c-myc promoter element predicts promoter strength.

abstract：:A cDNA from Arabidopsis thaliana resembling S-formylglutathione hydrolase (SFGH), an enzyme with putative roles in formaldehyde detoxification in animals and microorganisms, has been cloned and expressed in Escherichia coli. The purified recombinant Arabidopsis enzyme (AtSFGH) was a dimer composed of 31-kDa subunits. ...

journal_title：Archives of biochemistry and biophysics

authors： Kordic S,Cummins I,Edwards R

更新日期：2002-03-15 00:00:00

abstract：:This paper describes a theory for the mechanism of three-state transition of proteins which is often observed in aqueous organic cosolvent systems, i.e., from the native, via intermediate to helical forms. The first transition, accompanied by changes in the tertiary and/or secondary structures, was explained by larger...

journal_title：Archives of biochemistry and biophysics

authors： Arakawa T,Goddette D

更新日期：1985-07-01 00:00:00

abstract：:A microsomal retinol dehydrogenase (RoDH) that recognizes holo-cellular retinol binding protein (CRBP) as substrate is inhibited by phenylarsine oxide (IC50 = 3 microM) in the presence of 10 mM cysteine. Inhibition was reversible with dithiothreitol, indicating that two cysteine residues in close proximity are essenti...

journal_title：Archives of biochemistry and biophysics

authors： Boerman MH,Napoli JL

更新日期：1995-08-20 00:00:00

abstract：:X-ray crystallographic studies on glucose oxidase showed a strong interaction between carbohydrate and protein moieties of the glycoprotein. However, experimental studies under physiological conditions reported no influence of carbohydrate moiety on the structural and functional properties of glucose oxidase. In order...

journal_title：Archives of biochemistry and biophysics

authors： Akhtar MS,Bhakuni V

更新日期：2003-05-15 00:00:00

abstract：:CYP119 from Sulfolobus solfataricus is the first well-characterized thermophilic cytochrome P450 enzyme. The endogenous substrate for this enzyme is not known but it hydroxylates lauric acid in a reaction supported by surrogate mesophilic electron donors. However, reconstitution of a high-temperature catalytic system ...

journal_title：Archives of biochemistry and biophysics

authors： Puchkaev AV,Ortiz de Montellano PR

更新日期：2005-02-01 00:00:00

abstract：:The formation of alpha-hydroxyethyl radical from ethanol by deermouse microsomes supplemented with NADPH has been demonstrated with the EPR technique of spin trapping with alpha-(4-pyridyl-1-oxide)-N-t-butylnitrone (POBN) as the spin trap, in the presence of deferoxamine mesylate. Superoxide dismutase prevented the fo...

journal_title：Archives of biochemistry and biophysics

authors： Knecht KT,Thurman RG,Mason RP

更新日期：1993-06-01 00:00:00

abstract：:Extracellular ATP is known to increase the membrane permeability of a variety of cells. Addition of ATP to human leukemic lymphocytes loaded with the Ca2+ indicator, fura-2, induced a rise in cytosolic Ca2+ concentration which was attenuated or absent in NaCl media compared with KCl, choline Cl, or NMG Cl media. In co...

journal_title：Archives of biochemistry and biophysics

authors： Wiley JS,Chen R,Wiley MJ,Jamieson GP

更新日期：1992-02-01 00:00:00

abstract：:In hepatocytes incubated in the presence of 20 mM glucose, strong stimulation of glycogen synthesis by LiCl was observed in cells from both normal and streptozotocin-induced diabetic rats. This stimulation was concentration- and time-dependent, and it was related to an increase in the glycogen synthase activity ratio....

journal_title：Archives of biochemistry and biophysics

authors： Rodriguez-Gil JE,Guinovart JJ,Bosch F

更新日期：1993-03-01 00:00:00

abstract：:The hnRNP A/B family contains abundant nuclear proteins with major roles in alternative splicing and the ability for nucleo-cytoplasmic shuttling. Compared to the best known members of this family (hnRNP A1, A2/B1), hnRNP A3 is a relatively less known protein. We report herein immunochemical studies with the hnRNP A3 ...

journal_title：Archives of biochemistry and biophysics

authors： Papadopoulou C,Boukakis G,Ganou V,Patrinou-Georgoula M,Guialis A

更新日期：2012-07-15 00:00:00

abstract：:Estrogen-induced chick oviduct differentiation is accompanied by an increased capacity for protein glycosylation. A portion of this increase has been attributed to increased levels of dolichyl phosphate (Dol-P). Hormone withdrawal leads to an apparent decrease in Dol-P. Dol-P metabolism in the oviduct has been studied...

journal_title：Archives of biochemistry and biophysics

authors： DeRosa PA,Lucas JJ

更新日期：1984-11-01 00:00:00

abstract：:The relative reactivities of lysine residues of tropomyosin complexed with troponin have been measured in order to locate the binding site of troponin on tropomyosin in a complex between the two native proteins. The lysines were labeled with acetic anhydride using a competitive labeling procedure and the relative reac...

journal_title：Archives of biochemistry and biophysics

authors： Hitchcock-DeGregori SE,Lewis SF,Mistrik M

更新日期：1988-08-01 00:00:00

abstract：:A large portion of mitochondrial asparagine (Asn) is degraded by asparagine amino-transferase to produce alpha-ketosuccinamate (alpha KSA), which is then hydrolized by omega-amidase to produce oxaloacetate (OAA) and ammonia. This is in contrast to the catabolism in the cytosol, where the main catabolic route for Asn o...

journal_title：Archives of biochemistry and biophysics

authors： Moraga-Amador DA,MacPhee-Quiggley KM,Keefer JF,Schuster SM

更新日期：1989-01-01 00:00:00

abstract：:CYP3A9 is an estrogen-inducible ortholog of human liver CYP3A4 with 76.5% sequence identity to CYP3A4. Unlike CYP3A4, it is a very poor testosterone 6beta- and 2beta-hydroxylase, but a relatively better catalyst of progesterone monohydroxylation largely at 6beta, 16alpha, and 21 positions with negligible 6beta, 21-dih...

journal_title：Archives of biochemistry and biophysics

authors： Xue L,Zgoda VG,Arison B,Correia MA

更新日期：2003-01-01 00:00:00

abstract：:Purified NAD-malic enzyme from Ascaris suum is rapidly inactivated by the arginine reagent, 2,3-butanedione, and this inactivation is facilitated by 30 mM borate. Determination of the inactivation rate as a function of butanedione concentration suggests a second-order process overall, which is first order in butanedio...

journal_title：Archives of biochemistry and biophysics

authors： Rao GS,Kong CT,Benjamin RC,Harris BG,Cook PF

更新日期：1987-05-15 00:00:00

abstract：:The intrinsic fluorescence of potato tuber pyrophosphate:fructose-6-phosphate 1-phosphotransferase (PFP) was used as an indicator of conformational changes due to ligand binding. Binding of the substrates and the allosteric activator fructose-2,6-bisphosphate was quantitatively compared to their respective kinetic eff...

journal_title：Archives of biochemistry and biophysics

authors： Podestá FE,Plaxton WC

更新日期：2003-06-01 00:00:00

abstract：:pH dependence of the chemical reaction rates of p-bromophenacyl bromide (BPB) and of the binding constants of Ca2+ to bovine pancreatic active- and pro-phospholipases A2 (PLA2s) was studied at 25 degrees C and ionic strength 0.2. The pH dependence curves of the reaction rates of BPB with both enzymes were biphasic. Th...

journal_title：Archives of biochemistry and biophysics

authors： Fujii S,Meida M,Tani T,Inoue S,Iwama S,Katsumura S,Ikeda K

更新日期：1998-06-01 00:00:00

abstract：:Mammalian brains contain low levels of the Alzheimer amyloid precursor variants (AAPPs) and the normal form of the scrapie agent protease-resistant protein (PrPc); however, their mRNAs are readily detectable. To understand these discrepancies we have investigated some aspects of the translational regulation of these m...

journal_title：Archives of biochemistry and biophysics

authors： Denman R,Potempska A,Wolfe G,Ramakrishna N,Miller DL

更新日期：1991-07-01 00:00:00

abstract：:Since experiments with freshly isolated rat hepatocytes have shown that cellular vitamin E is consumed in response to insult by compounds that induce an oxidative stress only after cellular glutathione (GSH) concentrations have been substantially depleted, experiments were performed to determine whether this sequence ...

journal_title：Archives of biochemistry and biophysics

authors： Warren DL,Reed DJ

更新日期：1991-02-15 00:00:00

abstract：:In the present study steered molecular dynamics simulations were applied to investigate the unbinding process of the complex of human carbonic anhydrase with the natural HCO3(-) and promiscuous H2NCOHN(-) products. This process is crucial for restoring the catalytic cycle of the enzyme. This investigation set out to g...

journal_title：Archives of biochemistry and biophysics

authors： Piazzetta P,Marino T,Russo N

更新日期：2015-09-15 00:00:00

abstract：:Calycosin, a functional phytoestrogen isoflavone isolated from Radix astragali, has been shown to possess multiple pharmacological properties including anti-cancer activity. However, up to now, the anti-cancer effect and the related mechanism of calycosin on cervical cancer (CC) cells have not been explored. It has be...

journal_title：Archives of biochemistry and biophysics

authors： Zhang D,Sun G,Peng L,Tian J,Zhang H

更新日期：2020-09-30 00:00:00

abstract：:The gene of the human fatty acid omega-hydroxylase, CYP4A11, has been isolated from a human BAC library, and its complete genomic sequence has been determined. The CYP4A11 gene spanned 12,568 bp and contained 12 exons. The known PPAR recognition elements (PPRE), which were reported to be involved in the induction of C...

journal_title：Archives of biochemistry and biophysics

authors： Kawashima H,Naganuma T,Kusunose E,Kono T,Yasumoto R,Sugimura K,Kishimoto T

更新日期：2000-06-15 00:00:00

abstract：:Diets with high content of antioxidant polyphenols are associated with low prevalence of cardiovascular diseases and cancer. Inflammatory angiogenesis is a key pathogenic process both in cancer and atherosclerosis, and is tightly regulated by the proinflammatory enzyme cyclooxygenase (COX)-2 and the matrix degrading e...

journal_title：Archives of biochemistry and biophysics

authors： Scoditti E,Calabriso N,Massaro M,Pellegrino M,Storelli C,Martines G,De Caterina R,Carluccio MA

更新日期：2012-11-15 00:00:00

abstract：:The rat enhancer of split- and hairy-related protein-2 (SHARP-2) is a basic helix-loop-helix transcription factor. The hepatic expression of SHARP-2 mRNA was investigated under various conditions. The level was decreased in the regenerating rat liver and malignant hepatoma cells. In contrast, the expression of SHARP-2...

journal_title：Archives of biochemistry and biophysics

authors： Hirano S,Yamada K,Kawata H,Shou Z,Mizutani T,Shigematsu Y,Mayumi M,Miyamoto K

更新日期：2004-02-01 00:00:00

abstract：:Three iodinated derivatives of ubiquitin have been synthesized and these derivatives have been characterized in the ubiquitin-dependent protein degradation system. With chloramine-T as the oxidant, a derivative containing monoiodotyrosine is formed in the presence of 1 M KI and a derivative containing diiodotyrosine i...

journal_title：Archives of biochemistry and biophysics

authors： Cox MJ,Haas AL,Wilkinson KD

更新日期：1986-11-01 00:00:00

abstract：:Thymidylate synthase (TS) is an essential enzyme that must be expressed in all proliferating cells. The mouse TS promoter lacks a TATA box and an initiator element and initiates transcription over a broad region in cultured fibroblasts. The goal of this study was to determine if expression of the TS gene in a variety ...

journal_title：Archives of biochemistry and biophysics

authors： Lee Y,Shen G,Johnson LF

更新日期：1999-12-15 00:00:00

abstract：:The effects of orally administered secondary autoxidation products of linoleic acid in rat liver were investigated. Their administration led to two toxic effects on hepatic carbohydrate metabolism, as compared to the administration of saline or linoleic acid used as controls. One effect was depletion of glucose 6-phos...

journal_title：Archives of biochemistry and biophysics

authors： Ashida H,Kanazawa K,Minamoto S,Danno G,Natake M

更新日期：1987-11-15 00:00:00

abstract：:Complementary peptides to interleukin-2 (IL-2) sequences important for receptor binding were tested for their ability to mimic natural receptors and act as inhibitors of the IL-2/p55 receptor subunit interaction. Peptides hydropathically complementary to IL-2 sequences 15-27 and 40-54 were synthesized in a linear and ...

journal_title：Archives of biochemistry and biophysics

authors： Fassina G,Cassani G,Gnocchi P,Fornasiero MC,Isetta AM

更新日期：1995-04-01 00:00:00

abstract：:Hyaluronidase from fresh human serum was purified to apparent homogeneity in a two-step procedure. Potent serum inhibitors of hyaluronidase activity were removed during the course of the purification. Isolation of the enzyme was expedited by the use of a newly devised ELISA-like assay. Enzyme activity was measured by ...

journal_title：Archives of biochemistry and biophysics

authors： Afify AM,Stern M,Guntenhöner M,Stern R

更新日期：1993-09-01 00:00:00

abstract：:The sites of expression in the small intestine and the function of CYP2J4, a recently identified rat cytochrome (P450) isoform found to be predominantly expressed in the small intestine, were characterized. Immunoblot analysis with a polyclonal antibody to heterologously expressed CYP2J4 revealed that expression of CY...

journal_title：Archives of biochemistry and biophysics

authors： Zhang QY,Raner G,Ding X,Dunbar D,Coon MJ,Kaminsky LS

更新日期：1998-05-15 00:00:00

abstract：:GAPDH was speculated to function as a transient trap to reduce the potential toxicity of free heme by a specific and reversible binding with heme. Up to now, there has been lack of studies focused on this effect. In this paper, the efficiency of GAPDH-heme complex on catalyzing protein carbonylation and nitration, the...

journal_title：Archives of biochemistry and biophysics

authors： Huang Y,Zhang P,Yang Z,Wang P,Li H,Gao Z

更新日期：2017-04-01 00:00:00