当前位置： SCI文献检索 > ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS期刊下所有文献 > Characterization of tryptic hydrolysis of alpha-lactalbumin/saponin mixture and structural change of alpha-lactalbumin interacting with soybean saponin.

Characterization of tryptic hydrolysis of alpha-lactalbumin/saponin mixture and structural change of alpha-lactalbumin interacting with soybean saponin.

Abstract：

:Bovine milk alpha-lactalbumin (alpha-La) was mixed with soybean saponin, and the resulting mixture was hydrolyzed by trypsin. Saponin increased the tryptic-hydrolysis level of alpha-La only at relatively high phosphate buffer concentrations (> or = 0.05 M). T(1) experiments with acetylated soybean saponin demonstrated that there were some interactions between alpha-La and saponin not only at high concentrations of phosphate buffers but even at low concentrations as well. Circular dichroism spectra of alpha-La showed that the tertiary structure of alpha-La was changed through interactions with saponin only at high buffer concentrations. Furthermore, by analyzing the tryptic peptides from an alpha-La/saponin mixture, hydrolyzing rates at all or some of K5, R10, and K16 of alpha-La were accelerated by saponin interactions. The increase in the tryptic hydrolysis of alpha-La by saponin addition was considered due to modification of the tertiary structure of alpha-La by saponin.

journal_name

Arch Biochem Biophys

journal_title

Archives of biochemistry and biophysics

authors

Shimoyamada M,Okada Y,Watanabe K,Yamauchi R

doi

10.1016/j.abb.2005.01.001

subject

Has Abstract

pub_date

2005-03-15 00:00:00

pages

273-9

issue

2

eissn

0003-9861

issn

1096-0384

pii

S0003-9861(05)00002-0

journal_volume

435

pub_type

杂志文章

相关文献

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS文献大全
  • Enhancement of cytotoxicity of hydrogen peroxide by hyperthermia in chinese hamster ovary cells: role of antioxidant defenses.

    abstract：:Regional hyperthermia has potential for human cancer treatment, particularly in combination with systemic chemotherapy or radiotherapy. The mechanisms involved in heat-induced cell killing are currently unknown. Hyperthermia may increase oxidative stress in cells, and thus, oxidative stress could have a role in the me...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1998.1087

    authors： Lord-Fontaine S,Averill DA

    更新日期：1999-03-15 00:00:00

  • Modification of an arginine residue essential for the activity of NAD-malic enzyme from Ascaris suum.

    abstract：:Purified NAD-malic enzyme from Ascaris suum is rapidly inactivated by the arginine reagent, 2,3-butanedione, and this inactivation is facilitated by 30 mM borate. Determination of the inactivation rate as a function of butanedione concentration suggests a second-order process overall, which is first order in butanedio...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(87)90288-8

    authors： Rao GS,Kong CT,Benjamin RC,Harris BG,Cook PF

    更新日期：1987-05-15 00:00:00

  • Host cell signal transduction during Listeria monocytogenes infection.

    abstract：:The facultative intracellular bacterial pathogen Listeria monocytogenes invades and multiplies in many mammalian cell types. During the interaction with its host cells it strongly interferes with and modulates host cell functions. In the present review we summarize the current knowledge on the modulation of signal tra...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章,评审

    doi：10.1006/abbi.1999.1487

    authors： Kuhn M,Pfeuffer T,Greiffenberg L,Goebel W

    更新日期：1999-12-01 00:00:00

  • Cadmium-induced mRNA expression of Hsp32 is augmented in metallothionein-I and -II knock-out mice.

    abstract：:Cadmium is toxic and carcinogenic to humans and animals. The testis and lung are the target organs for cadmium carcinogenesis. Heat shock proteins (HSPs) as well as metallothionein (MT) and glutathione (GSH) play an important role in protection against its toxicity. HSP32, also known as heme oxygenase-1, is a 32-kDa p...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.2000.1997

    authors： Abe T,Yamamoto O,Gotoh S,Yan Y,Todaka N,Higashi K

    更新日期：2000-10-01 00:00:00

  • Fe-heme structure in Cu, Zn superoxide dismutase from Haemophilus ducreyi by X-ray absorption spectroscopy.

    abstract：:We have carried out an X-ray Absorption Spectroscopy (XAS) study of ferric, ferrous, CO- and NO-bound Haemophilus ducreyi Cu,ZnSOD (HdSOD) in solution to investigate the structural modifications induced by the binding of small gaseous ligands to heme in this enzyme. The combined analysis of EXAFS and XANES data has al...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2010.03.010

    authors： D'Angelo P,Zitolo A,Pacello F,Mancini G,Proux O,Hazemann JL,Desideri A,Battistoni A

    更新日期：2010-06-01 00:00:00

  • Reactivity of mu-peroxo-bridged dimeric vanadate in bromoperoxidation.

    abstract：:Diglycyl triperoxodivanadate [V2O2(O2)3(Gly H)2(H2O)2], a synthetic compound with mu-peroxo-bridge derived from H2O2 and vanadate, oxidized bromide to a bromination-competent intermediate in phosphate buffer and physiological pH. This is in contrast to the requirement of acid medium with H2O2 as the oxidant. Addition ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1997.0103

    authors： Rao AV,Islam NS,Ramasarma T

    更新日期：1997-06-15 00:00:00

  • Selectivity in ISG15 and ubiquitin recognition by the SARS coronavirus papain-like protease.

    abstract：:The severe acute respiratory syndrome coronavirus papain-like protease (SARS-CoV PLpro) carries out N-terminal processing of the viral replicase polyprotein, and also exhibits Lys48-linked polyubiquitin chain debranching and ISG15 precursor processing activities in vitro. Here, we used SDS-PAGE and fluorescence-based ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2007.07.006

    authors： Lindner HA,Lytvyn V,Qi H,Lachance P,Ziomek E,Ménard R

    更新日期：2007-10-01 00:00:00

  • Differential inactivation of rabbit and yeast triosephosphate isomerase: effect of oxidations produced by chloramine-T.

    abstract：:Triosephosphate isomerase from rabbit has 5 Cys and 2 Met, while triosephosphate isomerase from yeast has 2 Cys (present in the rabbit enzyme in equivalent positions) and no Met. Since chloramine-T oxidizes Cys and Met, we determined the effect it has on the activity and structure of both enzymes. The activity of trio...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1994.1395

    authors： Zubillaga RA,Pérez-Montfort R,Gómez-Puyou A

    更新日期：1994-09-01 00:00:00

  • The interaction of Trolox C, a water-soluble vitamin E analog, with ferrylmyoglobin: reduction of the oxoferryl moiety.

    abstract：:The oxidation of the heme iron of metmyoglobin by H2O2 yields an oxo ferryl complex (FeIV = O), similar to Compound II of peroxidases, as well as a protein radical; this high oxidation state of myoglobin is known as ferrylmyoglobin. The interaction of Trolox, a water-soluble vitamin E analog, with ferrylmyoglobin enta...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(92)90279-6

    authors： Giulivi C,Romero FJ,Cadenas E

    更新日期：1992-12-01 00:00:00

  • Dermatan is a better substrate for 4-O-sulfation than chondroitin: implications in the generation of 4-O-sulfated, L-iduronate-rich galactosaminoglycans.

    abstract：:The biosynthesis of dermatan sulfate is a complex process that involves, inter alia, formation of L-iduronic acid residues by C5-epimerization of D-glucuronic acid residues already incorporated into the growing polymer. It has been shown previously that this reaction is promoted by the presence of the sulfate donor 3'...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.2000.2043

    authors： Eklund E,Rodén L,Malmström M,Malmström A

    更新日期：2000-11-15 00:00:00

  • Cytochrome b5 oxidoreductase: expression and characterization of the original familial ideopathic methemoglobinemia mutations E255- and G291D.

    abstract：:NADH:cytochrome b5 oxidoreductase catalyzes the transfer of reducing equivalents from the physiological electron donor, NADH, to two molecules of cytochrome b5. Utilizing a heterologous expression system for the soluble, catalytic domain of the rat microsomal enzyme, we have produced two mutants, corresponding to E255...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2003.12.041

    authors： Davis CA,Barber MJ

    更新日期：2004-05-15 00:00:00

  • 1H, 13C, and 31P nuclear magnetic resonance spectral assignments for tobramycin, 2''-(adenosine-5'-phosphoryl)-tobramycin and 2''-(adenosine-5'-thiophosphoryl)-tobramycin.

    abstract：:Two enzymatically modified derivatives of tobramycin have been prepared by gentamicin nucleotidyl transferase-catalyzed adenylylation of tobramycin, using ATP and (Sp)-ATP alpha S as adenylylation substrates. (EC 2.7.7.46). The 1H, 13C, and 31P NMR spectra have been assigned for tobramycin, 2''-(adenosine-5'-phosphory...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(90)90331-r

    authors： Van Pelt JE,Mooberry ES,Frey PA

    更新日期：1990-08-01 00:00:00

  • Structural selectivity and molecular nature of L-glutamate transport in cultured human fibroblasts.

    abstract：:Uptake of L-[3H]glutamate by monolayers of fibroblasts cultured from human embryonic skin has been studied in the presence of several nonradioactive structural analogs of glutamate and aspartate. Results have suggested that the structural specificites of glutamate transporters in cultured human fibroblasts are similar...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1998.0626

    authors： Cooper B,Chebib M,Shen J,King NJ,Darvey IG,Kuchel PW,Rothstein JD,Balcar VJ

    更新日期：1998-05-15 00:00:00

  • Novel inhibitors of glutamyl-tRNA(Glu) reductase identified through cell-based screening of the heme/chlorophyll biosynthetic pathway.

    abstract：:The metabolite 5-aminolevulinic acid (ALA) is an early committed intermediate in the biosynthetic pathway of heme and chlorophyll formation. In plants, 5-aminolevulinic acid is synthesized via a two-step pathway in which glutamyl-tRNA(Glu) is reduced by glutamyl-tRNA(Glu) reductase (GluTR) to glutamate 1-semialdehyde,...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1999.1505

    authors： Loida PJ,Thompson RL,Walker DM,CaJacob CA

    更新日期：1999-12-15 00:00:00

  • The role of peroxisomal fatty acyl-CoA beta-oxidation in bile acid biosynthesis.

    abstract：:The physiological role of the peroxisomal fatty acyl-CoA beta-oxidizing system (FAOS) is not yet established. We speculated that there might be a relationship between peroxisomal degradation of long-chain fatty acids in the liver and the biosynthesis of bile acids. This was investigated using [1-14C]butyric acid and [...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(89)90473-6

    authors： Hayashi H,Miwa A

    更新日期：1989-11-01 00:00:00

  • 1,4-Benzoquinone reductase from basidiomycete Phanerochaete chrysosporium: spectral and kinetic analysis.

    abstract：:The reaction mechanism of a 1,4-benzoquinone reductase from the wood-rotting basidiomycete Phanerochaete chrysosporium was investigated. The native, oxidized, FMN-containing enzyme was reduced quantitatively by NADH and the resulting reduced enzyme was reoxidized in the presence of one equivalent of 2,6-di-methoxy-1,4...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1996.0279

    authors： Brock BJ,Gold MH

    更新日期：1996-07-01 00:00:00

  • Vitamin E slows the rate of free radical-mediated lipid peroxidation in cells.

    abstract：:Much of what is known about the antioxidant mechanism of vitamin E has been learned from studies of lipid dispersions, solutions, or subcellular organelles. We have investigated the effect of vitamin E supplementation on intact live eucaryotic cells. L1210 murine leukemia cells were exposed to an oxidative stress indu...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1996.0454

    authors： Wagner BA,Buettner GR,Burns CP

    更新日期：1996-10-15 00:00:00

  • Free cholesterol accumulation impairs antioxidant activities and aggravates apoptotic cell death in menadione-induced oxidative injury.

    abstract：:Although the relationship between hypercholesterolemia and oxidative stress has been extensively investigated, direct evidence regarding to the roles of cholesterol accumulation in the generations of reactive oxygen species (ROS) and apoptotic cell death under oxidative stress is lack. In this study, we investigated p...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2011.07.014

    authors： Lee W,Xu M,Li Y,Gu Y,Chen J,Wong D,Fung PC,Shen J

    更新日期：2011-10-01 00:00:00

  • Spontaneous porphyria of the Long-evans cinnamon rat: an animal model of Wilson's disease.

    abstract：:To confirm and extend our previous microspectrophotometric observations of 30-week-old male Long-Evans Cinnamon (LEC) rats, an animal model of human Wilson's disease, we analyzed the porphyrin patterns of the organs, urine, and plasma of LEC rats. Abnormal accumulation of porphyrins, especially highly carboxylated por...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1999.1641

    authors： Nakayama K,Takasawa A,Terai I,Okui T,Ohyama T,Tamura M

    更新日期：2000-03-15 00:00:00

  • The nature of enzyme-substrate complexes in acyl-coenzyme A dehydrogenases.

    abstract：:The nature of the purple complex formed upon the addition of octanoyl-CoA to the medium chain acyl-CoA dehydrogenase from pig kidney has been addressed by chemical quenching studies. Previous work, using quenching in 0.1 M KOH, suggested that the dehydrogenation product, trans-2-octenoyl-CoA, was not a participant in ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(88)90191-9

    authors： Lau SM,Thorpe C

    更新日期：1988-04-01 00:00:00

  • Oxidized aldose reductase: in vivo factor not in vitro artifact.

    abstract：:Characterization of aldose reductase purified from human placenta confirms that activation, as first analyzed in detail for the bovine enzyme, also occurs in humans. Routinely between 5 and 20% of the aldose reductase activity freshly purified from human placenta exhibits kinetic properties and insensitivity to aldose...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1996.0096

    authors： Grimshaw CE,Lai CJ

    更新日期：1996-03-01 00:00:00

  • Effect of dihydrocaffeic acid on UV irradiation of human keratinocyte HaCaT cells.

    abstract：:Dihydrocaffeic acid, a dietary constituent and a microbial metabolite of flavonoids, is an antioxidant, but few biological effects have been examined. After its production by microflora in the colon, dihydrocaffeic acid is absorbed and found in plasma as a combination of free and metabolized forms. Excess solar UV rad...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2008.01.019

    authors： Poquet L,Clifford MN,Williamson G

    更新日期：2008-08-15 00:00:00

  • Modes of inhibitory action of 4 beta-phorbol 12-myristate 13-acetate in thrombin-stimulated arachidonic acid release in intact and permeabilized platelets.

    abstract：:The tumor-promoting phorbol ester 4 beta-phorbol 12-myristate 13-acetate (PMA) inhibited thrombin-stimulated arachidonic acid (AA) release in rabbit and human platelets. PMA was effective over the same concentration range that activates protein kinase C in intact rabbit platelets: IC50 vs thrombin = 0.5 nM, greater th...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/0003-9861(90)90021-p

    authors： Murayama T,Kajiyama Y,Takahashi A,Nomura Y

    更新日期：1990-01-01 00:00:00

  • TMX, a human transmembrane oxidoreductase of the thioredoxin family: the possible role in disulfide-linked protein folding in the endoplasmic reticulum.

    abstract：:Various proteins sharing thioredoxin (Trx)-like active site sequences (Cys-Xxx-Xxx-Cys) have been found and classified in the Trx superfamily. Among them, transmembrane Trx-related protein (TMX) was recently identified as a novel protein possessing an atypical active site sequence, Cys-Pro-Ala-Cys. In the present stud...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2003.11.003

    authors： Matsuo Y,Nishinaka Y,Suzuki S,Kojima M,Kizaka-Kondoh S,Kondo N,Son A,Sakakura-Nishiyama J,Yamaguchi Y,Masutani H,Ishii Y,Yodoi J

    更新日期：2004-03-01 00:00:00

  • Molecular diversity of calpastatin in human erythroid cells.

    abstract：:According to differences in mobility on SDS-polyacrylamide gel electrophoresis, calpastatins (inhibitor proteins of the calcium-dependent proteinase calpain) are classified into the tissue type (100-120 kDa) and the erythrocyte type (70 kDa), which lacks the amino-terminal domains (domains L and 1). We investigated th...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1993.1294

    authors： Takano E,Nosaka T,Lee WJ,Nakamura K,Takahashi T,Funaki M,Okada H,Hatanaka M,Maki M

    更新日期：1993-06-01 00:00:00

  • Glutamate binding triggers monomerization of unliganded mGluR2 dimers.

    abstract：:The Metabotropic glutamate receptor 2 (mGluR2) is involved in several neurological and psychiatric disorders and is an attractive drug target. It is believed to form a strict dimer and the dimeric assembly is necessary for glutamate induced activation. Although many studies have focused on glutamate induced conformati...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2020.108632

    authors： Singh DR,Pandey K,Mishra AK,Pandey P,Vivcharuk V

    更新日期：2021-01-15 00:00:00

  • Functionally conservative substitutions at cardiac troponin I S43/45.

    abstract：:A phospho-null Ala substitution at protein kinase C (PKC)-targeted cardiac troponin I (cTnI) S43/45 reduces myocyte and cardiac contractile function. The goal of the current study was to test whether cTnIS43/45N is an alternative, functionally conservative substitution in cardiac myocytes. Partial and more extensive e...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2016.02.002

    authors： Lang SE,Stevenson TK,Xu D,O'Connell R,Westfall MV

    更新日期：2016-07-01 00:00:00

  • Endothelium-derived nitric oxide (NO) activates the NO-epidermal growth factor receptor-mediated signaling pathway in bradykinin-stimulated angiogenesis.

    abstract：:Nitric oxide (NO) is involved in angiogenesis and stimulates the EGF-R signaling pathway. Stimulation of different endothelial cell lines with bradykinin (BK) activates the endothelial NO synthase (eNOS) and promotes EGF-R tyrosine phosphorylation. Increase in NO production correlated with enhanced phosphorylation of ...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2014.06.011

    authors： Moraes MS,Costa PE,Batista WL,Paschoalin T,Curcio MF,Borges RE,Taha MO,Fonseca FV,Stern A,Monteiro HP

    更新日期：2014-09-15 00:00:00

  • Post-translational modifications of myofilament proteins involved in length-dependent prolongation of relaxation in rabbit right ventricular myocardium.

    abstract：:The phosphorylation state of several cardiac myofilament proteins changes with the level of stretch in intact, twitch-contracting cardiac muscles. It remains unclear which kinases are involved in the length-dependent phosphorylation of these proteins. We set out to investigate which kinases are involved after a step-w...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1016/j.abb.2012.10.005

    authors： Monasky MM,Taglieri DM,Jacobson AK,Haizlip KM,Solaro RJ,Janssen PM

    更新日期：2013-07-01 00:00:00

  • Characterization of a novel carboxypeptidase produced by the entomopathogenic fungus Metarhizium anisopliae.

    abstract：:Preparative isoelectric focusing and gel filtration chromatography were used to purify a carboxypeptidase produced by the entomopathogenic fungus Metarhizium anisopliae during growth on cockroach cuticle. The enzyme was inhibited by diisopropyl fluorophosphate, implying involvement of a serine residue in catalysis. Ho...

    journal_title：Archives of biochemistry and biophysics

    pub_type： 杂志文章

    doi：10.1006/abbi.1994.1458

    authors： St Leger RJ,Bidochka MJ,Roberts DW

    更新日期：1994-11-01 00:00:00