The fertilization layer mediated block to polyspermy in Xenopus laevis: isolation of the cortical granule lectin ligand.

Abstract:

:The fertilization layer of Xenopus laevis eggs is formed by the cortical granule lectin binding to its ligand. The binding requires Ca2+, is specific for galactose, and functionally establishes a block to polyspermy at fertilization. We have designed a new enzyme-linked lectin assay for the cortical granule lectin (CGL) ligand which can detect the presence of the CGL ligand at a sensitivity of 1-2 ng/ml. This assay is specifically inhibited with galactose, 50% inhibition at 9.9 mM, and produces a linear response between 2 and 20 ng of jelly adsorbed to the microtiter plate. Using this assay, the CGL ligand was purified through gel filtration, anion-exchange, and CGL affinity chromatography. Hydrolysis of the purified CGL ligand with a series of exoglycosidases showed that a terminal alpha-galactose is the ligand structure required for recognition by CGL.

journal_name

Arch Biochem Biophys

authors

Quill TA,Hedrick JL

doi

10.1006/abbi.1996.0398

subject

Has Abstract

pub_date

1996-09-15 00:00:00

pages

326-32

issue

2

eissn

0003-9861

issn

1096-0384

pii

S0003-9861(96)90398-7

journal_volume

333

pub_type

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