Isoflurane's effect on interfacial dynamics in GAPDH influences methylglyoxal reactivity.

Abstract:

:Diabetic surgical patients are at risk for peri- and post-operative complications, which can be prevented by maintaining tight glycemic control during anesthesia. Control of blood sugar would decrease unwanted chemical reactions, such as protein glycation, minimizing tissue dysfunction. Methylglyoxal (MG) is a major contributor to protein modification and tissue dysfunction seen in diabetic patients. We hypothesized that inhaled anesthetics may play a role in protein glycation and examined the effects of isoflurane on MG-induced modification of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Isoflurane promoted MG-induced modification of GAPDH as evidenced by an increase in fluorescent glycation products, a change in chromatographic elution patterns and a loss of enzyme activity. Isoflurane's effect may be mediated by altering interfacial events. Our working model involves the binding of isoflurane to GAPDH, increasing the susceptibility to MG-induced modification of residues involved in oligomerization. These findings suggest a molecular basis for maintaining glycemic control during anesthesia.

journal_name

Arch Biochem Biophys

authors

Pattin AE,Ochs S,Theisen CS,Fibuch EE,Seidler NW

doi

10.1016/j.abb.2010.04.001

subject

Has Abstract

pub_date

2010-06-01 00:00:00

pages

7-12

issue

1

eissn

0003-9861

issn

1096-0384

pii

S0003-9861(10)00123-2

journal_volume

498

pub_type

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