Biosynthetic mechanism of ribulose-1,5-bisphosphate carboxylase in the purple photosynthetic bacterium, Chromatium vinosum. III. Absence of extrachromosomal DNA.

abstract：:The effect of modulating AP-1 activity on the levels of induced erythroid and megakaryocytic differentiation of the erythroleukemia cell line K562 was examined. Cells were stably transfected with expression vectors encoding either a tetracycline-responsive dominant negative c-Jun (JunDN) or a hybrid Raf protein induci...

journal_title：Archives of biochemistry and biophysics

authors： Rosson D,O'Brien TG

更新日期：1998-04-15 00:00:00

abstract：:Sequence alignment of pig mitochondrial NADP-dependent isocitrate dehydrogenase with eukaryotic (human, rat, and yeast) and Escherichia coli isocitrate dehydrogenases reveals that Tyr316 is completely conserved and is equivalent to the E. coli Tyr345, which interacts with the 2'-phosphate of NADP in the crystal struct...

journal_title：Archives of biochemistry and biophysics

authors： Lee P,Colman RF

更新日期：2002-05-01 00:00:00

abstract：:We have previously produced 12 monoclonal antibodies (MAbs) against rat cytochrome P450(CYP)2B1, and 8 of these inhibit CYP2B1 catalytic activity to varying extents. Using competitive binding studies we showed that this collection of 12 MAbs recognize at least 6 spatially distinct epitopes. The complete coding sequenc...

journal_title：Archives of biochemistry and biophysics

authors： Parimoo B,Thomas PE

更新日期：2000-08-01 00:00:00

abstract：:A search of the chicken expressed sequence tag (EST) database identified 2 cDNA clones that appeared to represent members of the SULT1B and SULT1C enzyme families. These cDNAs were fully sequenced and found to contain full-length inserts. Phylogenetic analysis of the derived amino acid sequences clearly placed them as...

journal_title：Archives of biochemistry and biophysics

authors： Wilson LA,Reyns GE,Darras VM,Coughtrie MW

更新日期：2004-08-01 00:00:00

abstract：:Among the biologically and pharmacologically relevant nitrogen oxides, nitroxyl (HNO) remains one of the most poorly studied and least understood. Several previous reports indicate that thiols may be a primary target for the biological actions of HNO. However, the intimate details of the chemical interaction of HNO wi...

journal_title：Archives of biochemistry and biophysics

authors： Cook NM,Shinyashiki M,Jackson MI,Leal FA,Fukuto JM

更新日期：2003-02-01 00:00:00

abstract：:The vesicle-to-micelle structural transitions that occurred in the interaction of sodium dodecyl sulfate with phosphatidylcholine vesicles were studied at the equilibrium by means of dynamic light scattering (at different scattering angles) and freeze-fracture electron microscopy techniques. The incorporation of surfa...

journal_title：Archives of biochemistry and biophysics

authors： López O,Cócera M,Wehrli E,Parra JL,de la Maza A

更新日期：1999-07-15 00:00:00

abstract：:Much of what is known about the antioxidant mechanism of vitamin E has been learned from studies of lipid dispersions, solutions, or subcellular organelles. We have investigated the effect of vitamin E supplementation on intact live eucaryotic cells. L1210 murine leukemia cells were exposed to an oxidative stress indu...

journal_title：Archives of biochemistry and biophysics

authors： Wagner BA,Buettner GR,Burns CP

更新日期：1996-10-15 00:00:00

abstract：:It was found that the rate of polymerization of G-actin increased with the decrease of ATP concentration. When excess ATP was replaced by chloride through anion-exchange treatment, the extent of actin polymerization did not change provided that the ionic strength was raised immediately after the treatment. In the mean...

journal_title：Archives of biochemistry and biophysics

authors： Fung BM,Eyob E

更新日期：1983-02-01 00:00:00

abstract：:Glycosphingolipids were extracted from human semen and purified. Based on the fluorometric assay of sphingosine, in spermatozoa a content of 4.4 +/- 0.9 nmol/10(8) cells of gangliosides and 22.1 +/- 1.7 nmol/10(8) cells of neutral glycosphingolipids was determined. Seminal plasma contained 4.1 +/- 0.6 nmol ganglioside...

journal_title：Archives of biochemistry and biophysics

authors： Ritter G,Krause W,Geyer R,Stirm S,Wiegandt H

更新日期：1987-09-01 00:00:00

abstract：:Lipid homeostasis in liver is known to be altered with diabetes mellitus, ultimately leading to liver damage and related complications. The present work aimed to evaluate changes in the liver phospholipid profile after 4 months of uncontrolled hyperglycemia. Twenty Wistar rats were divided into two groups: control and...

journal_title：Archives of biochemistry and biophysics

authors： Simões C,Domingues P,Ferreira R,Amado F,Duarte JA,Vitorino R,Neuparth MJ,Nunes C,Rocha C,Duarte I,Domingues MR

更新日期：2013-10-15 00:00:00

abstract：:Yeast (Saccharomyces cerevisiae) alcohol dehydrogenase I (SceADH) binds NAD+ and NADH less tightly and turns over substrates more rapidly than does horse (Equus caballus) liver alcohol dehydrogenase E isoenzyme (EcaADH), and neither enzyme uses NADP efficiently. Amino acid residues in the proposed adenylate binding po...

journal_title：Archives of biochemistry and biophysics

authors： Fan F,Plapp BV

更新日期：1999-07-15 00:00:00

abstract：:Tyrosine kinase was extracted from rat spleen. Some 60% of the activity with angiotensin as the substrate was found in the cytoplasmic supernatant obtained at 100,000g. Two kinases in the supernatant, TKI and -II were purified 170- and 600-fold, respectively, by column chromatography. The molecular weights of TKI and ...

journal_title：Archives of biochemistry and biophysics

authors： Hasuma T,Inoue A,Higashi Y,Morisawa S,Yukioka M

更新日期：1990-07-01 00:00:00

abstract：:Thymidylate synthase (TS) is an essential enzyme that must be expressed in all proliferating cells. The mouse TS promoter lacks a TATA box and an initiator element and initiates transcription over a broad region in cultured fibroblasts. The goal of this study was to determine if expression of the TS gene in a variety ...

journal_title：Archives of biochemistry and biophysics

authors： Lee Y,Shen G,Johnson LF

更新日期：1999-12-15 00:00:00

abstract：:Exposure of MCF-7 breast cancer cells to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) causes an elevated cytochrome P450 content and a marked increase in the microsomal hydroxylation of 17 beta-estradiol (E2) at the C-2, C-4, C-15 alpha, and C-6 alpha positions. In this study we investigated the involvement of cytochrom...

journal_title：Archives of biochemistry and biophysics

authors： Spink DC,Eugster HP,Lincoln DW 2nd,Schuetz JD,Schuetz EG,Johnson JA,Kaminsky LS,Gierthy JF

更新日期：1992-03-01 00:00:00

abstract：:The native conformation of bovine retinal arrestin has been characterized by a variety of spectroscopic methods. The purified protein gives rise to a near uv absorption band centered at 279 nm which results from the absorbance of its 14 tyrosine and one tryptophan residue. The extinction coefficient for this absorptio...

journal_title：Archives of biochemistry and biophysics

authors： Kotake S,Hey P,Mirmira RG,Copeland RA

更新日期：1991-02-15 00:00:00

abstract：:The ferric and ferrous forms of bovine erythrocyte green hemeprotein react with hydroperoxides to form higher oxidation state intermediates with absorbance maxima in the Soret region at 426 and 422 nm, respectively. In the absence of an appropriate reductant, these intermediates undergo rapid bleaching reactions. 2,2'...

journal_title：Archives of biochemistry and biophysics

authors： Xu F,DeFilippi LJ,Ballou DP,Hultquist DE

更新日期：1993-02-15 00:00:00

abstract：:Selenoprotein I (SELENOI) is an ethanolamine phosphotransferase that catalyzes the third reaction of the Kennedy pathway for the synthesis of phosphatidylethanolamine. Since the role of SELENOI in murine embryogenesis has not been investigated, SELENOI-/+ mating pairs were used to generate global KO offspring. Of 323 ...

journal_title：Archives of biochemistry and biophysics

authors： Avery JC,Yamazaki Y,Hoffmann FW,Folgelgren B,Hoffmann PR

更新日期：2020-08-15 00:00:00

abstract：:The visible and near-uv absorption and circular dichroic spectra were determined for spinach and poplar plastocyanin under a variety of conditions. The visible spectra showed that the copper center was invariant to changes in species, chemical modification with ethylenediamine, and addition of high concentrations of s...

journal_title：Archives of biochemistry and biophysics

authors： Draheim JE,Anderson GP,Pan RL,Rellick LM,Duane JW,Gross EL

更新日期：1985-02-15 00:00:00

abstract：:Brazzein is a 54-amino-acid sweet-tasting protein first isolated from the fruit of Pentadiplandra brazzeana Baillon found in West Africa. Brazzein, as isolated from the fruit, is 500 times sweeter than sucrose on a weight basis (9500 times sweeter on a per-molecule basis). A minor component of brazzein from fruit, des...

journal_title：Archives of biochemistry and biophysics

authors： Assadi-Porter FM,Aceti DJ,Cheng H,Markley JL

更新日期：2000-04-15 00:00:00

abstract：:Some properties and subcellular localization of adenosine diphosphatase (ADPase) activity from rat heart have been investigated. The pH optimum was 7.4, maximal activity was found with 5 mM MgCl2, and the apparent Km was 20 microM. ADPase activity was strongly inhibited by NaF and AppNHp, and to a lesser extent by AMP...

journal_title：Archives of biochemistry and biophysics

authors： de Vente J,Velema J,Zaagsma J

更新日期：1984-08-15 00:00:00

abstract：:The partial degradation of proteoglycan aggregate by human leucocyte elastase yielded products that banded with Mr 190,000, 140,000, 88,000, and 71,000 when analyzed by sodium dodecyl sulfate (SDS)-polyacrylamide electrophoresis. Analysis of these bands revealed that the 190,000- and 140,000-Da bands contained chondro...

journal_title：Archives of biochemistry and biophysics

authors： Mok MT,Ilic MZ,Handley CJ,Robinson HC

更新日期：1992-02-01 00:00:00

abstract：:The effect of hypothyroidism on kinetic characteristics of cytochrome oxidase in rat heart mitochondria was studied. Mitochondrial preparations from control and hypothyroid rats had equivalent Km values for cytochrome c, while the maximal activity of the oxidase was significantly decreased (more than 30%) in mitochond...

journal_title：Archives of biochemistry and biophysics

authors： Paradies G,Ruggiero FM,Dinoi P,Petrosillo G,Quagliariello E

更新日期：1993-11-15 00:00:00

abstract：:Steady-state kinetic studies of the bovine carbonic anhydrase B-catalyzed hydration of CO2, dehydration of HCO3-, and hydrolysis of p-nitrophenylacetate were made in glycerol/water solvents of increased viscosity in order that the effect of diffusion-control on the substrate association reactions could be determined. ...

journal_title：Archives of biochemistry and biophysics

authors： Hasinoff BB

更新日期：1984-09-01 00:00:00

abstract：:Rabbit skeletal muscle sarcoplasmic reticulum (SR) was fractionated by isopycnic density gradient centrifugation into longitudinal tubules (LSR) and terminal cisternae (TC). Junctional face membranes (JFM) were obtained by Triton X-100 treatment of the TC fraction (Costello, B., Chadwick, C., Saito, A., Chu, A., Maure...

journal_title：Archives of biochemistry and biophysics

authors： Volpe P,Gutweniger HE,Montecucco C

更新日期：1987-02-15 00:00:00

abstract：:The intracellular location of pyruvate carboxylase (EC 6.4.1.1) in rat liver and Saccharomyces cerevisiae was investigated using the antibody-gold and protein A-gold techniques carried out as a postembedding immunoelectron microscopic procedure. The vast majority of gold particles (greater than 98%), indicative of the...

journal_title：Archives of biochemistry and biophysics

authors： Rohde M,Lim F,Wallace JC

更新日期：1991-10-01 00:00:00

abstract：:Aryl hydrocarbon hydroxylase activity is most closely associated with cytochrome P-450c in the rat and cytochrome P1-450 in the mouse. The sequence for the orthologous human gene coding for this enzymatic activity has been determined from several sources: cytochrome P-450c isolated from human embryonic DNA [K. Kawajir...

journal_title：Archives of biochemistry and biophysics

authors： Iversen PL,Heiger WJ,Bresnick E,Hines RN

更新日期：1987-07-01 00:00:00

abstract：:Uridine phosphorylase is one of the critical enzymes in the pyrimidine salvage pathway. Cells regenerate uridine for nucleotide metabolism by incorporating uracil with ribose-1-phosphate with this enzyme. Recent studies indicate that Escherichia coli uridine phosphorylase is destabilized in the presence of ATP. Howeve...

journal_title：Archives of biochemistry and biophysics

authors： Liu YK,Lin TH,Liu PF

更新日期：2017-11-15 00:00:00

abstract：:Adaptive responses to the oxidative stress of hydrogen peroxide (H2O2) were studied in the yeast Saccharomyces cerevisciae strain RZ53. Our results show that the growth of naive cells is readily arrested by H2O2 challenge. In contrast, cells that have been preexposed to relatively low H2O2 priming treatments (i.e., ce...

journal_title：Archives of biochemistry and biophysics

authors： Davies JM,Lowry CV,Davies KJ

更新日期：1995-02-20 00:00:00

abstract：:We investigated whether expression of non-selective cation channels of the transient receptor potential canonical (TRPC) channel family are associated with proinflammatory cytokines in monocytes. Using quantitative RT-PCR we studied the expression of TRPC3, interleukin-1beta (IL-1beta), and tumor necrosis factor-alpha...

journal_title：Archives of biochemistry and biophysics

authors： Thilo F,Scholze A,Liu DY,Zidek W,Tepel M

更新日期：2008-03-01 00:00:00

abstract：:Studies were carried out at pH 7.0 and gamma/2 0.15 before addition of CaCl2 or EDTA. Clotting time, tau, at 3.03 microM fibrinogen and 0.91 u/ml thrombin was determined for equilibrium systems. With added Ca2+, tau decreases, from tau 0 at 0 added Ca2+ (mean, 29.7 +/- 3 s), by approximately 3 s at 5 mM added Ca2+. Wi...

journal_title：Archives of biochemistry and biophysics

authors： Perizzolo KE,Sullivan S,Waugh DF

更新日期：1985-03-01 00:00:00