Abstract:
:Selenoprotein I (SELENOI) is an ethanolamine phosphotransferase that catalyzes the third reaction of the Kennedy pathway for the synthesis of phosphatidylethanolamine. Since the role of SELENOI in murine embryogenesis has not been investigated, SELENOI-/+ mating pairs were used to generate global KO offspring. Of 323 weanling pups, no homozygous KO genotypes were found. E6.5-E18.5 embryos (165 total) were genotyped, and only two E18.5 KO embryos were detected with no discernable anatomical defects. To screen embryos prior to uterine implantation that occurs ~ E6, blastocyst embryos (E3.5-E4.4) were flushed from uteruses of pregnant females and analyzed for morphology and genotype. KO embryos were detected in 5 of 6 pregnant females, and 7 of the 32 genotyped embryos were found to be SELENOI KO that exhibited no overt pathological features. Overall, these results demonstrate that, except for rare cases (2/490 = 0.4%), global SELENOI deletion leads to early embryonic lethality.
journal_name
Arch Biochem Biophysjournal_title
Archives of biochemistry and biophysicsauthors
Avery JC,Yamazaki Y,Hoffmann FW,Folgelgren B,Hoffmann PRdoi
10.1016/j.abb.2020.108444subject
Has Abstractpub_date
2020-08-15 00:00:00pages
108444eissn
0003-9861issn
1096-0384pii
S0003-9861(20)30453-7journal_volume
689pub_type
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