Abstract:
:Cytochrome P450 enzymes are involved in a number of steps in ecdysteroid (moulting hormone) homeostasis in insects. We report the cloning and characterization of an ecdysteroid agonist-responsive cytochrome P450, CYP18A1, from the cotton leafworm, Spodoptera littoralis. Northern blot analysis showed that the mRNA transcript was expressed at times of increasing ecdysteroid titre in final instar S. littoralis larvae and was induced by the ecdysteroid receptor agonist, RH-5992, in midgut and fat body. In addition, transcript expression was also detected in the prothoracic glands, a major ecdysteroid biosynthetic tissue, in both S. littoralis and the tobacco hornworm, Manduca sexta, at a time of increasing ecdysteroid titre. The exact significance of the temporal and spatial expression of CYP18A1 is unclear. The characterization of a P450 that is ecdysteroid agonist-responsive may provide a future target for exploitation in the development of novel insect control strategies.
journal_name
Arch Biochem Biophysjournal_title
Archives of biochemistry and biophysicsauthors
Davies L,Williams DR,Turner PC,Rees HHdoi
10.1016/j.abb.2006.06.016subject
Has Abstractpub_date
2006-09-01 00:00:00pages
4-12issue
1eissn
0003-9861issn
1096-0384pii
S0003-9861(06)00227-Xjournal_volume
453pub_type
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pub_type: 杂志文章
doi:10.1006/abbi.2000.2055
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pub_type: 杂志文章
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更新日期:2014-12-15 00:00:00
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pub_type: 杂志文章
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
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更新日期:2011-03-15 00:00:00
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pub_type: 杂志文章
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更新日期:2018-04-02 00:00:00
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pub_type: 杂志文章
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更新日期:2009-05-15 00:00:00
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pub_type: 杂志文章
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更新日期:2006-04-15 00:00:00
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
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更新日期:2004-02-15 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1016/0003-9861(85)90528-4
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pub_type: 杂志文章
doi:10.1016/0003-9861(84)90556-3
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pub_type: 杂志文章
doi:10.1006/abbi.1994.1105
更新日期:1994-03-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2010-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2012-02-01 00:00:00
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
doi:10.1006/abbi.1996.0477
更新日期:1996-11-01 00:00:00
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pub_type: 杂志文章
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
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更新日期:2008-01-01 00:00:00
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
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更新日期:1987-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:1983-07-15 00:00:00
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pub_type: 杂志文章
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更新日期:1992-05-01 00:00:00
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journal_title:Archives of biochemistry and biophysics
pub_type: 杂志文章
doi:10.1016/j.abb.2017.07.012
更新日期:2017-09-15 00:00:00