Two splenic soluble tyrosine kinases from the rat.

abstract：:Small compounds that activate the insulin-dependent signaling pathway have potential therapeutic applications in controlling type 2 diabetes mellitus. The rat enhancer of split- and hairy-related protein-2 (SHARP-2) is an insulin-inducible transcription factor that decreases expression of the phosphoenolpyruvate carbo...

journal_title：Archives of biochemistry and biophysics

authors： Haneishi A,Takagi K,Asano K,Yamamoto T,Tanaka T,Nakamura S,Noguchi T,Yamada K

更新日期：2012-09-01 00:00:00

abstract：:Truncation of 112 amino acids at the N-terminus (Nd(1-112)) changes the chain transfer pattern of the Escherichia coli glycogen branching enzyme (GBE) [Arch. Biochem. Biophys. 397 (2002) 279]. We investigated further the role of the N-terminus by engineering other truncated GBEs and analyzing the branching pattern by ...

journal_title：Archives of biochemistry and biophysics

authors： Devillers CH,Piper ME,Ballicora MA,Preiss J

更新日期：2003-10-01 00:00:00

abstract：:A 1.1-kb Hp alpha I fragment of the Escherichia coli chromosome containing the gene for translation initiation factor 3 was employed as a probe in heterologous hybridization to chromosomal DNA from a variety of other procaryotes. Positive hybridization was observed to DNA derived from all gram-negative bacteria tested...

journal_title：Archives of biochemistry and biophysics

authors： Pramanik A,Schwartz I

更新日期：1984-11-15 00:00:00

abstract：:The intrinsic fluorescence of potato tuber pyrophosphate:fructose-6-phosphate 1-phosphotransferase (PFP) was used as an indicator of conformational changes due to ligand binding. Binding of the substrates and the allosteric activator fructose-2,6-bisphosphate was quantitatively compared to their respective kinetic eff...

journal_title：Archives of biochemistry and biophysics

authors： Podestá FE,Plaxton WC

更新日期：2003-06-01 00:00:00

abstract：:Heme and iron are essential to almost all forms of life. The strict maintenance of heme and iron homeostasis is essential to prevent cellular toxicity and the existence of systemic and intracellular regulation is fundamental. Cytosolic heme can be catabolized and detoxified by heme oxygenases (HOs). Interestingly, fre...

journal_title：Archives of biochemistry and biophysics

authors： Yanatori I,Richardson DR,Toyokuni S,Kishi F

更新日期：2019-09-15 00:00:00

abstract：:Human soluble epoxide hydrolase (hsEH) has been shown to play a role in regulating blood pressure and inflammation. HsEH consists of an N-terminal phosphatase and a C-terminal epoxide hydrolase domain. In the present study, we examined the effects of polymorphisms in the hsEH gene on phosphatase activity, enzyme stabi...

journal_title：Archives of biochemistry and biophysics

authors： Srivastava PK,Sharma VK,Kalonia DS,Grant DF

更新日期：2004-07-15 00:00:00

abstract：:Spinach chloroplast NAD(P)-glyceraldehyde-3-phosphate dehydrogenase (NAD(P)-GAPDH; EC, 1.2.1.13) was purified as the 600-kDa oligomer of low specific activity. Incubation of the enzyme with either a reductant or a 1,3-bisphosphoglycerate (1,3bisPGA) generating system, but most effectively with both, resulted in an inc...

journal_title：Archives of biochemistry and biophysics

authors： Baalmann E,Backhausen JE,Rak C,Vetter S,Scheibe R

更新日期：1995-12-20 00:00:00

abstract：:The important characteristic of novel Mimosa pudica tubulin is its ability to bind colchicine only when dithiothreitol is included in the isolation buffer, indicating the involvement of sulfhydryl groups in colchicine binding. Modification of sulfhydryl groups by a sulfhydryl modifying agent also affects the normal as...

journal_title：Archives of biochemistry and biophysics

authors： Roychaudhuri A,Biswas S

更新日期：1992-05-01 00:00:00

abstract：:We tested the effects of a series of synthetic oligonucleotides (hybridons) complementary to the 5' noncoding and coding regions of rabbit beta-globin mRNA on endogenous protein synthesis in a rabbit reticulocyte cell-free translation system. With highly purified hybridons inhibition was completely specific for beta-g...

journal_title：Archives of biochemistry and biophysics

authors： Goodchild J,Carroll E 3rd,Greenberg JR

更新日期：1988-06-01 00:00:00

abstract：:Recombinant glutathione S-transferase P (GST-P) was purified in a homogeneous state. Fatty acid analysis of the enzyme revealed that the final enzyme preparation endogenously bound fatty acids, mostly palmitic acid or stearic acid, which were difficult to dissociate from the complex. Temperature-dependent analysis by ...

journal_title：Archives of biochemistry and biophysics

authors： Nishihira J,Ishibashi T,Sakai M,Tsuda S,Hikichi K

更新日期：1993-04-01 00:00:00

abstract：:Solubilized NADPH-cytochrome c (P450) reductase was purified to homogeneity from an extract of spearmint (Mentha spicata) glandular trichomes by dye-ligand interaction chromatography on Matrex-Gel Red A and affinity chromatography on 2', 5'-adenosine diphosphate agarose. SDS-PAGE of the purified enzyme preparation rev...

journal_title：Archives of biochemistry and biophysics

authors： Ponnamperuma K,Croteau R

更新日期：1996-05-01 00:00:00

abstract：:The protein sequence encoded by a creatine transporter cDNA cloned from a human heart library was identical to that cloned from a human kidney library (Nash et al., Receptors Channels 2, 165-174, 1994), except that at position 285 the former contained an Ala residue and the latter contained a Pro residue. Expression o...

journal_title：Archives of biochemistry and biophysics

authors： Dai W,Vinnakota S,Qian X,Kunze DL,Sarkar HK

更新日期：1999-01-01 00:00:00

abstract：:The glutamine synthetase (GS) gene is expressed at high levels in several cell types, including astrocytes, pericentral hepatocytes, and adipocytes. During hormone-mediated adipocyte differentiation of 3T3-L1 cells, GS gene expression increases several hundred fold. We previously reported that elements in the distal 5...

journal_title：Archives of biochemistry and biophysics

authors： Hadden TJ,Ryou C,Zhu L,Miller RE

更新日期：2002-01-15 00:00:00

abstract：:The motility of actin filaments interacting with heavy meromyosin molecules was directly observed on indium tin oxide-coated glass (ITO-glass), over which a surface current flowed. Because the increase in surface current applied to ITO-glass increases the temperature, we focused on the temperature-dependence of the sl...

journal_title：Archives of biochemistry and biophysics

authors： Wada R,Sato D,Nakamura T,Hatori K

更新日期：2015-11-15 00:00:00

abstract：:The electron paramagnetic resonance (EPR) signal of the tyrosyl radical attributed to ribonucleoside diphosphate reductase decreases after treatment of promyelocytic leukemic HL60 cells with 2-formylpyridine thiosemicarbazonato copper(II) (CuL). According to EPR studies, CuL forms adducts with both histidine and cyste...

journal_title：Archives of biochemistry and biophysics

authors： Narasimhan J,Antholine WE,Chitambar CR,Petering DH

更新日期：1991-09-01 00:00:00

abstract：:The partial degradation of proteoglycan aggregate by human leucocyte elastase yielded products that banded with Mr 190,000, 140,000, 88,000, and 71,000 when analyzed by sodium dodecyl sulfate (SDS)-polyacrylamide electrophoresis. Analysis of these bands revealed that the 190,000- and 140,000-Da bands contained chondro...

journal_title：Archives of biochemistry and biophysics

authors： Mok MT,Ilic MZ,Handley CJ,Robinson HC

更新日期：1992-02-01 00:00:00

abstract：:The essential Zn(II) in bovine liver dihydropyrimidine amidohydrolase (DHPase) was removed by incubation with 2,6-dipicolinic acid and replaced with Mn(II). Electron paramagnetic resonance studies of Mn(II) binding show that there are four binding sites per tetramer, and the dissociation constant at pH 7.5 is 13.5 mic...

journal_title：Archives of biochemistry and biophysics

authors： Lee MH,Pettigrew DW,Sander EG,Nowak T

更新日期：1987-12-01 00:00:00

abstract：:The three-dimensional conformation of a protein provides a wealth of biochemical information and with the advent of cloning techniques that allow the preparation of proteins almost at will, a renewed interest has arisen in the crystallographic determination of protein structures. As in any research technique, however,...

journal_title：Archives of biochemistry and biophysics

authors： Holden HM,Rayment I

更新日期：1991-11-15 00:00:00

abstract：:Maturation of the precursor forms of bovine cholesterol side-chain cleavage cytochrome P-450 (P-450SCC) and 11 beta-hydroxylase cytochrome P-450 (P-450(11)beta) was investigated using mitochondria from bovine corpus luteum. The results show that both precursors, whose synthesis was directed by bovine adrenocortical RN...

journal_title：Archives of biochemistry and biophysics

authors： Matocha MF,Waterman MR

更新日期：1986-11-01 00:00:00

abstract：:Antimicrobial and cell-penetrating peptides have been the object of extensive studies for more than 60 years. Initially these two families were studied separately, and more recently parallels have been drawn. These studies have given rise to numerous methodological developments both in terms of observation techniques ...

journal_title：Archives of biochemistry and biophysics

authors： Emmanuelle S,Astrid W,Sandrine S,Sophie C,Nicolas R

更新日期：2021-01-07 00:00:00

abstract：:Dystrophic chicken breast muscle mitochondria contain significantly less mitochondrial creatine kinase than normal breast muscle mitochondria. Breast muscle mitochondria from normal 16- to 40-day-old chickens contain approximately 80 units of mitochondrial creatine kinase per unit of succinate:INT (p-iodonitrotetrazol...

journal_title：Archives of biochemistry and biophysics

authors： Bennett VD,Hall N,DeLuca M,Suelter CH

更新日期：1985-07-01 00:00:00

abstract：:The development of enzyme and buffer premixes for in vitro biotransformation assays is described. The protein premixes contain a mixture of three recombinant human proteins, cytochrome P450 (P450) 3A4, NADPH-P450 reductase, cytochrome b5, and liposomes. The buffer premix contains reagents which, when diluted, provide ...

journal_title：Archives of biochemistry and biophysics

authors： Shaw PM,Hosea NA,Thompson DV,Lenius JM,Guengerich FP

更新日期：1997-12-01 00:00:00

abstract：:Tetrahydro-dUMP, an analog of the putative transition state in aminohydrolysis of deoxycytidine monophosphate (dCMP) inhibits the allosteric enzyme deoxycytidylate aminohydrolase with high affinity. The inhibition is reversible, and its kinetics is consistent with the analog binding at the substrate site only to one a...

journal_title：Archives of biochemistry and biophysics

authors： Nucci R,Febbraio F,Piccialli G,de Napoli L,Vaccaro C,Rossi M,Whitehead EP

更新日期：1994-04-01 00:00:00

abstract：:Glucose-6-phosphatase is a multicomponent system located in the endoplasmic reticulum, involving both a catalytic subunit (G6PC) and several substrate and product carriers. The glucose-6-phosphate carrier is called G6PT1. Using light scattering, we determined K(D) values for phosphate and glucose transport in rat live...

journal_title：Archives of biochemistry and biophysics

authors： Clottes E,Middleditch C,Burchell A

更新日期：2002-12-01 00:00:00

abstract：:Carcinoembryonic antigen (CEA) binds to an 80-kDa cell surface receptor on Kupffer cells via the peptide sequence PELPK (residues 108-112) located at the hinge region between the N and Al immunoglobulin-like domains. This study is aimed at analyzing the specificity of the peptide binding, determining biodistribution o...

journal_title：Archives of biochemistry and biophysics

authors： Gangopadhyay A,Thomas P

更新日期：1996-10-01 00:00:00

abstract：:Activation of the NLRP3 inflammasome plays an important role in high glucose- induced endothelial dysfunction in patients with type 2 diabetes mellitus (T2DM). Dulaglutide, a newly developed glucagon-like peptide-1 receptor (GLP-1R) agonist, has been approved for the management of T2DM. In the current study, we aimed ...

journal_title：Archives of biochemistry and biophysics

authors： Luo X,Hu Y,He S,Ye Q,Lv Z,Liu J,Chen X

更新日期：2019-08-15 00:00:00

abstract：:Organic acidurias (OADs) are inherited disorders of amino acid metabolism biochemically characterized by accumulation of short-chain carboxylic acids in tissues and biological fluids of the affected patients and clinically by predominant neurological manifestations. Some of these disorders are amenable to treatment, w...

journal_title：Archives of biochemistry and biophysics

authors： Wajner M,Vargas CR,Amaral AU

更新日期：2020-12-15 00:00:00

abstract：:In the present study, the hepatic microsomal and peroxisomal bifunctional trans-2-enoyl CoA hydratases were isolated and purified from rats treated with 2% di-(2-ethylhexyl)phthalate for 8 days. These two enzymes (microsomal and peroxisomal) were purified with the identical purification procedures and had identical mo...

journal_title：Archives of biochemistry and biophysics

authors： Cook L,Ghesquier D,Nagi MN,Favreau LV,Cinti DL

更新日期：1987-02-01 00:00:00

abstract：:Actinomycin D (act-D) anchors itself into DNA-base pairs by intercalation and thereby inhibits mRNA synthesis. It has been well established that act-D elicits apoptosis in various cell types involving endothelial cells. However, the regulatory mechanisms of actinomycin D-induced apoptotic cell death still remain uncle...

journal_title：Archives of biochemistry and biophysics

authors： Shim D,Kang HY,Jeon BW,Kang SS,Chang SI,Kim HY

更新日期：2004-05-15 00:00:00

abstract：:3-Hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase exists in interconvertible active and inactive forms in cultured fibroblasts from normal and familial hypercholesterolemic subjects. The inactive form can be activated by endogenous or added phosphoprotein phosphatase. Active or partially active HMG-CoA reducta...

journal_title：Archives of biochemistry and biophysics

authors： Beg ZH,Reznikov DC,Avigan J

更新日期：1986-01-01 00:00:00