Abstract:
:An in vitro acrosome-like reaction was induced in spermatozoa from the boar cauda epididymis by incubation in Tyrode's solution containing 1 mg/ml fatty acid-free bovine serum albumin. Plasma membranes were isolated from the spermatozoa at different times during the incubation and analyzed for their lipid composition. The total lipid, phospholipid, and glycolipid content of the membranes did not change during the acrosome-like reaction, whereas the amount of diacylglycerols and free fatty acids increased. Within the phospholipid class, a decrease of the inositol phospholipid and and sphingomyelin content was observed, whereas the other phospholipids of the plasma membranes did not decrease significantly after 2 h of incubation. Changes in the sterol composition of the membranes were also observed. The onset of the lipid changes was correlated with the uptake of extracellular calcium by the spermatozoa. These results for the lipid changes in isolated sperm plasma membranes during an in vitro acrosome reaction provide the first direct evidence that a modulation of the plasma membrane lipid composition is involved in an acrosome-like reaction of mammalian spermatozoa.
journal_name
Arch Biochem Biophysjournal_title
Archives of biochemistry and biophysicsauthors
Nikolopoulou M,Soucek DA,Vary JCdoi
10.1016/0003-9861(86)90698-3subject
Has Abstractpub_date
1986-10-01 00:00:00pages
30-7issue
1eissn
0003-9861issn
1096-0384pii
0003-9861(86)90698-3journal_volume
250pub_type
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