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Filamentous actin binding ability of cortactin isoforms is responsible for their cell-cell junctional localization in epithelial cells.

Abstract：

:Cortactin is an F-actin binding protein that contributes to cytoskeleton remodelling. We identified five isoforms of mouse cortactin that differ in the number of tandem 37-amino acid repeats, named cortactin repeats. The transcription of minor isoforms with 4.5, 3.5 or 2.5 cortactin repeats was low in most adult tissues whereas an isoform with 4.5 cortactin repeats was highly transcribed in the adult brain. In accordance with the brain-specific upregulation of a minor isoform, a brain-specific novel 72-kDa cortactin protein was identified. Major isoforms with 6.5 or 5.5 cortactin repeats bound F-actin more robustly than minor isoforms in vitro. All isoforms were concentrated at cell-cell junction sites in epithelial cells. Deletion mutants lacking whole cortactin repeats did not bind F-actin and were not concentrated at cell-cell junction sites. Thus, the F-actin binding ability is mostly correlated with the number of cortactin repeats and is required for the cell-cell junctional localization.

journal_name

Arch Biochem Biophys

journal_title

Archives of biochemistry and biophysics

authors

Katsube T,Togashi S,Hashimoto N,Ogiu T,Tsuji H

doi

10.1016/j.abb.2004.04.015

subject

Has Abstract

pub_date

2004-07-01 00:00:00

pages

79-90

issue

1

eissn

0003-9861

issn

1096-0384

pii

S0003986104002310

journal_volume

427

pub_type

杂志文章

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