The multi-ligand binding first family 35 Carbohydrate Binding Module (CBM35) of Clostridium thermocellum targets rhamnogalacturonan I.

abstract：:The present paper reports a quantitative investigation on the binding of aluminum to human serum albumin. Equilibrium dialysis and a general thermodynamic approach have been used to determine the binding parameters. Two aluminum binding sites have been identified on the albumin molecule, involving sites with single an...

journal_title：Archives of biochemistry and biophysics

authors： Zatta P,Dalla Via L,Di Noto V

更新日期：2003-09-01 00:00:00

abstract：:We studied the effect of glycoprotein GPIIb/IIIa (integrin alpha IIb beta 3) receptor occupancy by adenosine 5',1-thiotriphosphate (ATP alpha S), a competitive inhibitor of the ADP receptor, by fibrinogen, and by peptides containing the RGD (Arg-Gly-Asp) sequence as RGDW (Arg-Gly-Asp-Trp), RGDS (Arg-Gly-Asp-Ser), or t...

journal_title：Archives of biochemistry and biophysics

authors： Padoin E,Alexandre A,Cavallini L,Polverino de Laureto P,Rao GH,Doni MG

更新日期：1996-09-15 00:00:00

abstract：:Superoxide generation, assessed as the rate of acetylated cytochrome c reduction inhibited by superoxide dismutase, by purified NADPH cytochrome P-450 reductase or intact rat liver microsomes was found to account for only a small fraction of their respective NADPH oxidase activities. DTPA-Fe3+ and EDTA-FE3+ greatly st...

journal_title：Archives of biochemistry and biophysics

authors： Morehouse LA,Thomas CE,Aust SD

更新日期：1984-07-01 00:00:00

abstract：:The influence of transmembrane ion fluxes on mycoplasma membrane potentials was studied. Electric membrane potential was calibrated vs fluorescence intensity of a potential-sensitive carbocyanine dye according to delta psi = (RT/F) X log([aIN(1 - IN) - b]/Kint), where IN = I/I0, I0 = maximal fluorescence intensity (ob...

journal_title：Archives of biochemistry and biophysics

authors： Schummer U,Schiefer HG

更新日期：1986-02-01 00:00:00

abstract：:Inactivations of chicken liver pyruvate carboxylase with N-(7-dimethylamino-4-methyl-3-coumarinyl)maleimide (DACM) and o-phthalaldehyde (o-PA) have identified cysteine and lysine residues that are essential for catalytic activity. Protection experiments suggest that the modified residues are located in or near the fir...

journal_title：Archives of biochemistry and biophysics

authors： Werneburg BG,Ash DE

更新日期：1993-06-01 00:00:00

abstract：:The objective of this study was to develop straightforward processes that could be applied to the large-scale production of β-cryptoxanthin in an attempt to facilitate investigation of its biological activity. An oleoresin obtained from crude extracts of marigold flowers (Tagetes erecta) with approximately 24% total l...

journal_title：Archives of biochemistry and biophysics

authors： Khachik F

更新日期：2018-09-01 00:00:00

abstract：:We have previously shown that exposure of Clone 9 cells to hypoxia, cyanide, or azide results in an acute stimulation of glucose transport that is largely mediated by "activation" of glucose transporter (Glut1) sites preexisting in the plasma membrane. However, it is not known whether inhibition of oxidative phosphory...

journal_title：Archives of biochemistry and biophysics

authors： Hamrahian AH,Zhang JZ,Elkhairi FS,Prasad R,Ismail-Beigi F

更新日期：1999-08-15 00:00:00

abstract：:The Ca2+-ATPase activity of isolated membranes and purified plasma membrane ATPase from pig brain was measured in the presence of specific inhibitors. The inhibition of the enzymatic activity by vanadate presents a lower affinity in microsomes than in the synaptic plasma membrane vesicles, showing K0.5 of 0.4 and 0.2 ...

journal_title：Archives of biochemistry and biophysics

authors： Salvador JM,Mata AM

更新日期：1998-03-15 00:00:00

abstract：:Glucosylsphingosine is a potent inhibitor of lysosomal glucocerebrosidase and the broad-specificity, cytosolic beta-glucosidase of human liver. In the present study, it was demonstrated that the broad-specificity beta-glucosidase was also inhibited by galactosylsphingosine. The inhibition was observed when the enzyme ...

journal_title：Archives of biochemistry and biophysics

authors： LaMarco KL,Glew RH

更新日期：1985-02-01 00:00:00

abstract：:The decomposition of S-nitrosoglutathione (GSNO) in the presence of Cu2+ and glutathione (GSH) was studied by stopped-flow/rapid-scan spectroscopy. Reduction of Cu2+ by GSH and subsequent formation of a GS-*Cu+ complex occurred within 200 ms, with the amount of complex formed depending on the GSH-to-Cu2+ ratio. The ra...

journal_title：Archives of biochemistry and biophysics

authors： Gorren AC,Schrammel Astrid,Schmidt K,Mayer B

更新日期：1996-06-15 00:00:00

abstract：:Triosephosphate isomerase from rabbit has 5 Cys and 2 Met, while triosephosphate isomerase from yeast has 2 Cys (present in the rabbit enzyme in equivalent positions) and no Met. Since chloramine-T oxidizes Cys and Met, we determined the effect it has on the activity and structure of both enzymes. The activity of trio...

journal_title：Archives of biochemistry and biophysics

authors： Zubillaga RA,Pérez-Montfort R,Gómez-Puyou A

更新日期：1994-09-01 00:00:00

abstract：:While prior studies focusing on male mice suggest a role for sterol carrier protein-2/sterol carrier protein-x (SCP-2/SCP-x; DKO) on hepatic phytol metabolism, its role in females is unresolved. This issue was addressed using female and male wild-type (WT) and DKO mice fed a phytoestrogen-free diet without or with 0.5...

journal_title：Archives of biochemistry and biophysics

authors： McIntosh AL,Storey SM,Huang H,Kier AB,Schroeder F

更新日期：2017-12-01 00:00:00

abstract：:Pyruvate dehydrogenase (E1), an alpha(2)beta(2) tetramer, catalyzes the oxidative decarboxylation of pyruvate and reductive acetylation of lipoyl moieties of the dihydrolipoamide acetyltransferase. The roles of betaW135, alphaP188, alphaM181, alphaH15, and alphaR349 of E1 determined by kinetic analysis were reassessed...

journal_title：Archives of biochemistry and biophysics

authors： Korotchkina LG,Ciszak EM,Patel MS

更新日期：2004-09-15 00:00:00

abstract：:A microsomal preparation from seedlings of Zea mays catalyzed the NAD+-dependent oxidative decarboxylation of several substrates, including 4alpha-carboxy-cholest-7-en-3beta-ol, synthesized according to a new procedure, giving the first in vitro evidence for this enzymatic activity in a higher plant. A GC assay has be...

journal_title：Archives of biochemistry and biophysics

authors： Rondet S,Taton M,Rahier A

更新日期：1999-06-15 00:00:00

abstract：:The single-stranded, DNA-dependent ATPase activity of purified recA protein was found to be inhibited competitively by ribose-modified analogs of ATP, 3'-O-anthraniloyl-ATP (Ant-ATP), and 3'-O-(N-methylanthraniloyl)-ATP (Mant-ATP). The Ki values for Ant-ATP and Mant-ATP were around 7 and 3 microM at pH 7.5, respective...

journal_title：Archives of biochemistry and biophysics

authors： Karasaki Y,Higashi K

更新日期：1984-09-01 00:00:00

abstract：:Sarcolemmal vesicles of white and red skeletal muscles of the rabbit were prepared by consecutive density gradient centrifugations in sucrose and dextran according to Seiler and Fleischer (1982, J. Biol. Chem. 257, 13,862-13,871). White and red muscle membrane fractions enriched in sarcolemma were characterized by hig...

journal_title：Archives of biochemistry and biophysics

authors： Wetzel P,Gros G

更新日期：1990-06-01 00:00:00

abstract：:Desulfovibrio vulgaris rubredoxin, which contains a single [Fe(SCys)4] site, is shown to be a catalytically competent electron donor to two enzymes from the same organism, namely, rubrerythrin and two-iron superoxide reductase (a.k.a. rubredoxin oxidoreductase or desulfoferrodoxin). These two enzymes have been implica...

journal_title：Archives of biochemistry and biophysics

authors： Coulter ED,Kurtz DM Jr

更新日期：2001-10-01 00:00:00

abstract：:The use of affinity chromatography on wheat germ agglutinin columns to partially purify detergent extracts of muscarinic acetylcholine receptor from porcine atria is described. The procedure results in a 20-fold purification of the protein. The partially purified protein binds [3H]L-QNB (the L isomer of quinuclidinyl ...

journal_title：Archives of biochemistry and biophysics

authors： Herron GS,Schimerlik MI

更新日期：1984-05-01 00:00:00

abstract：:The thermodynamic parameters of the alkaline transition for oxidized native yeast iso-1 cytochrome c and Rhodopseudomonas palustris cytochrome c(2) (cytc(2)) have been determined through direct electrochemistry experiments carried out at variable pH and temperature and compared to those for horse and beef heart cytoch...

journal_title：Archives of biochemistry and biophysics

authors： Battistuzzi G,Borsari M,Ranieri A,Sola M

更新日期：2002-08-15 00:00:00

abstract：:The Xin repeat-containing proteins, Xinα (Xirp1) and Xinβ (Xirp2), localize to the intercalated discs (ICDs) of mammalian hearts. Mouse Xinα (mXinα) directly interacts with β-catenin and actin filaments, potentially coupling the N-cadherin/β-catenin complexes to the underlying actin cytoskeleton and modulating ICD int...

journal_title：Archives of biochemistry and biophysics

authors： Wang Q,Lu TL,Adams E,Lin JL,Lin JJ

更新日期：2013-07-01 00:00:00

abstract：:Although an elastolytic activity in eosinophil-rich cell fractions from mice has been reported, this enzyme has not been purified and characterized as yet in any mammalian species. Eosinophilic elastase was isolated from human eosinophil fragments (cytosomes) obtained from normal and eosinophilic subjects. The enzyme ...

journal_title：Archives of biochemistry and biophysics

authors： Lungarella G,Menegazzi R,Gardi C,Spessotto P,de Santi MM,Bertoncin P,Patriarca P,Calzoni P,Zabucchi G

更新日期：1992-01-01 00:00:00

abstract：:A new creatine analog, 1-carboxyethyl-2-iminoimidazolidine (homocyclocreatine), has been synthesized and compared with other synthetic analogs of creatine as a substrate for creatine kinase under both in vitro and in vivo conditions. Reactivity with rabbit muscle creatine kinase at 2 mM and pH 7.0 occurred in the orde...

journal_title：Archives of biochemistry and biophysics

authors： Roberts JJ,Walker JB

更新日期：1983-02-01 00:00:00

abstract：:The appearance of a high molecular weight gelatinolytic enzyme (230 kDa) correlated with cartilage collagen loss in chick embryonic tibias cultured with lipopolysaccharide. This 230 kDa enzyme was purified and its activity was measured on synthetic and natural substrates. The enzyme was activated by aminophenylmercuri...

journal_title：Archives of biochemistry and biophysics

authors： Patchigolla RK,Knudson W,Schmid TM

更新日期：2012-04-01 00:00:00

abstract：:Mitochondrial metabolism depends on movement of hydrophilic metabolites through the mitochondrial outer membrane via the voltage-dependent anion channel (VDAC). Here we assessed VDAC permeability of intracellular mitochondria in cultured hepatocytes after plasma membrane permeabilization with 8 microM digitonin. Block...

journal_title：Archives of biochemistry and biophysics

authors： Holmuhamedov E,Lemasters JJ

更新日期：2009-01-15 00:00:00

abstract：:A theoretical and experimental study has been made of the advancing elution profile in frontal gel chromatography of interacting systems for which the elution volume of the complex is smaller than that of the larger reactant. First, Gilbert-Jenkins theory is used to delineate the form of the elution profile from the m...

journal_title：Archives of biochemistry and biophysics

authors： Cann JR,Winzor DJ

更新日期：1987-07-01 00:00:00

abstract：:[(3)H]8-OH-DPAT is a selective ligand for labeling 5-HT(1A) receptor sites. In competition binding experiments, we found that classic biogenic amine transporter inhibitors displaced [(3)H]8-OH-DPAT binding at its high-affinity binding sites in HeLaS3 cells. [(125)I]RTI-55 and [(3)H]paroxetine are known to specifically...

journal_title：Archives of biochemistry and biophysics

authors： Feng JJ,Cheng FC,Lin CH,Wei JW,Yang SD

更新日期：2011-02-01 00:00:00

abstract：:The rate constant for quenching, kq, of the phosphorescence of 6-bromo-2-naphthyl sulfate (BNS) by cytochromes c, cytochrome c peroxidase, catalase, and myoglobin has been measured as a function of temperature and solvent viscosity. In aqueous solution at pH 7.0 for cytochromes c and myoglobin the value of kq is nearl...

journal_title：Archives of biochemistry and biophysics

authors： Beckham S,Cook MP,Karki L,Luchsinger MM,Whitlock VR,Wu Y,Zhang Q,Schuh MD

更新日期：1994-05-01 00:00:00

abstract：:The purpose of the current study is to present further evidence for prothrombin self-association as assessed by chemical crosslinking. When the self-association (evaluated by covalent crosslinking with dithiobis(succinimidylpropionate) of prothrombin or fragment 1 was evaluated at the same molar concentration of prote...

journal_title：Archives of biochemistry and biophysics

authors： Tarvers RC,Roberts HR,Straight DL,Featherstone GL,Lundblad RL

更新日期：1987-09-01 00:00:00

abstract：:A number of natural or synthetic compounds as AGE inhibitors have been proposed, discovered or currently being advanced by others and us. We have identified two new classes of aromatic compounds; aryl- (and heterocyclic) ureido and aryl (and heterocyclic) carboxamido phenoxyisobutyric acids, and benzoic acid derivativ...

journal_title：Archives of biochemistry and biophysics

authors： Rahbar S,Figarola JL

更新日期：2003-11-01 00:00:00

abstract：:The effects of GSH on the autoxidation of the fava bean pyrimidine aglycones, divicine and isouramil, and on acid-hydrolyzed vicine (provisional identification 2-amino-4,5,6-trihydroxypyrimidine) have been studied. GSH alone promoted redox cycling of each compound, with concomitant GSH oxidation and H2O2 production. I...

journal_title：Archives of biochemistry and biophysics

authors： Winterbourn CC

更新日期：1989-06-01 00:00:00