Expression, purification, and initial characterization of human Yes protein tyrosine kinase from a bacterial expression system.

abstract：:Concentrations of GDP, which are expected to bind to the catalytic site and inhibit the autophosphorylation of succinyl-CoA synthetase (SCS) when NTP is used as a substrate, were found to increase the level of phosphoenzyme formed. The ability of GDP to do so is dependent upon the presence of a protein distinct from S...

journal_title：Archives of biochemistry and biophysics

authors： Birney M,Um H,Klein C

更新日期：1997-11-01 00:00:00

abstract：:Coupling of the narrow 311-nm Gd3+ fluorescence transition with the vibrational transitions of its inner-sphere ligands gives rise to the emission of vibronic photons between 320 and 350 nm. The energy difference between the fluorescence and vibronic transitions is equal to that of the vibrational transitions of the m...

journal_title：Archives of biochemistry and biophysics

authors： MacGregor RB Jr

更新日期：1989-10-01 00:00:00

abstract：:Hepatocytes from fed rats were incubated for 120 min in the presence of alpha-D-[1,2-13C]glucose pentaacetate (1.7 mM), both D-[1,2-13C]glucose (1.7 mM) and acetate (8.5 mM), alpha-D-glucose penta[2-13C]acetate (1.7 mM), or D-[1,2-13C]glucose (8.3 mM). The amounts of 13C-enriched L-lactate and D-glucose and those of a...

journal_title：Archives of biochemistry and biophysics

authors： Malaisse WJ,Ladrière L,Kadiata MM,Verbruggen I,Willem R

更新日期：2000-09-01 00:00:00

abstract：:The fatty acid composition of total lipids and the adrenoceptor-mediated activation of glycogenolysis were studied in isolated hepatocytes from rats maintained on a control diet or on an essential fatty acid (EFA)-free diet. In cells from rats on the EFA-free diet there was a marked reduction in linoleic and arachidon...

journal_title：Archives of biochemistry and biophysics

authors： Grojec MS,Ishac EJ,Kapocsi J,Kunos G

更新日期：1990-11-15 00:00:00

abstract：:Although in vitro models are often used in beta-carotene research, knowledge about the uptake and metabolism of beta-carotene in cell lines is lacking. We measured by HPLC the intracellular levels of beta-carotene and its metabolites in 9 human intestinal and lung cell lines after exposure to 1 microM beta-carotene du...

journal_title：Archives of biochemistry and biophysics

authors： Franssen-van Hal NL,Bunschoten JE,Venema DP,Hollman PC,Riss G,Keijer J

更新日期：2005-07-01 00:00:00

abstract：:In this study, the binding points of MethB and two structurally-related cationic phenoxazine dyes [meldola blue (MB) and nile blue (NB)] to human butyrylcholinesterase (BChE) were investigated by molecular docking and site directed mutagenesis. The comparative inhibitory effects of MethB, MB and NB on recombinant wild...

journal_title：Archives of biochemistry and biophysics

authors： Sezgin Z,Biberoglu K,Chupakhin V,Makhaeva GF,Tacal O

更新日期：2013-04-01 00:00:00

abstract：:Cytochrome P450 (P450) 2D6 is the classic human liver debrisoquine 4-hydroxylase, the first human P450 for which genetic polymorphism was clearly demonstrated. We prepared 11 different constructs of P450 2D6, with modification at the N-terminus, for expression in Escherichia coli with the vector pCW. These varied cons...

journal_title：Archives of biochemistry and biophysics

authors： Gillam EM,Guo Z,Martin MV,Jenkins CM,Guengerich FP

更新日期：1995-06-01 00:00:00

abstract：:The thermodynamic parameters of the alkaline transition for oxidized native yeast iso-1 cytochrome c and Rhodopseudomonas palustris cytochrome c(2) (cytc(2)) have been determined through direct electrochemistry experiments carried out at variable pH and temperature and compared to those for horse and beef heart cytoch...

journal_title：Archives of biochemistry and biophysics

authors： Battistuzzi G,Borsari M,Ranieri A,Sola M

更新日期：2002-08-15 00:00:00

abstract：:Using the polymerase chain reaction (PCR) a 0.8-kb product was amplified from cDNA made from the midgut and Malpighian tubules of fifth instar larvae of Helicoverpa virescens. This 0.8-kb PCR product was then used to isolate a clone of the B subunit of the V-type ATPase from a cDNA library made from the same tissues. ...

journal_title：Archives of biochemistry and biophysics

authors： Gill SS,Ross LS

更新日期：1991-11-15 00:00:00

abstract：:Appropriate dietary interventions may reduce the potentially damaging effects of free radicals generated during metabolism and various physiological conditions. We have investigated the effects of dietary vitamins C, E, beta-carotene, or selenium (Se) on the activity of endogenous antioxidant enzymes and respiratory c...

journal_title：Archives of biochemistry and biophysics

authors： Desai VG,Casciano D,Feuers RJ,Aidoo A

更新日期：2001-10-15 00:00:00

abstract：:Methyl acetyl phosphate, which was originally synthesized as a site-specific reagent for hydroxybutyrate dehydrogenase [R. Kluger and W.-C. Tsui (1980) J. Org. Chem. 45, 2723], also has an affinity for the binding site for 2,3-diphosphoglycerate in hemoglobin. Three residues in or near this cleft between the beta-chai...

journal_title：Archives of biochemistry and biophysics

authors： Ueno H,Pospischil MA,Manning JM,Kluger R

更新日期：1986-02-01 00:00:00

abstract：:Vanadate V(V) markedly stimulated the oxidation of NADPH by GSSG reductase and this oxidation was accompanied by the consumption of O2 and the accumulation of H2O2. Superoxide dismutases completely eliminated this effect of V(V), whereas catalase was without effect, as was exogenous H2O2 added to 0.1 mM. These effects...

journal_title：Archives of biochemistry and biophysics

authors： Liochev SI,Fridovich I

更新日期：1992-05-01 00:00:00

abstract：:During freezing in sodium and potassium phosphate (NaP and KP) buffer solutions, changes in pH may impact the stability of proteins. Since the degradation pathways for the model proteins, monomeric and tetrameric beta-galactosidase (beta-gal), chosen for this study are governed by conformational changes (i.e., physica...

journal_title：Archives of biochemistry and biophysics

authors： Pikal-Cleland KA,Rodríguez-Hornedo N,Amidon GL,Carpenter JF

更新日期：2000-12-15 00:00:00

abstract：:The hepatic cytosolic hemoprotein tryptophan 2,3-dioxygenase (TDO) is the rate-limiting enzyme in tryptophan catabolism and thus plays a key role in regulating the physiological flux of tryptophan into relevant metabolic pathways. The TDO protein is induced by corticosteroids such as dexamethasone (DEX) and is stabili...

journal_title：Archives of biochemistry and biophysics

authors： Ren S,Correia MA

更新日期：2000-05-01 00:00:00

abstract：:The 3-dimensional structure determination of rat phenylalanine hydroxylase (PAH) has identified potentially important amino acids lining the active site cleft with the majority of these having hydrophobic side-chains including several with aromatic side chains. Here we have analyzed the effect on rat PAH enzyme kineti...

journal_title：Archives of biochemistry and biophysics

authors： Jennings IG,Cotton RG,Kobe B

更新日期：2000-12-15 00:00:00

abstract：:The effects of GSH on the autoxidation of the fava bean pyrimidine aglycones, divicine and isouramil, and on acid-hydrolyzed vicine (provisional identification 2-amino-4,5,6-trihydroxypyrimidine) have been studied. GSH alone promoted redox cycling of each compound, with concomitant GSH oxidation and H2O2 production. I...

journal_title：Archives of biochemistry and biophysics

authors： Winterbourn CC

更新日期：1989-06-01 00:00:00

abstract：:Insulin has pleiotropic effects on sensitive cells, including the regulation of specific mRNA accumulation initiated by the binding of insulin to its plasma membrane receptor. Lectins, such as wheat germ agglutinin (WGA) and concanavalin A (Con A), are known to be insulin mimetic. It is thought that WGA and Con A inte...

journal_title：Archives of biochemistry and biophysics

authors： Messina JL,Hamlin J,Larner J

更新日期：1987-04-01 00:00:00

abstract：:Biochemical communication between the cytoplasmic and mitochondrial subsystems of the cell depends on solute carriers in the mitochondrial inner membrane that transport metabolites between the two compartments. We have expressed and purified a yeast mitochondrial carrier protein (Mtm1p, YGR257cp), originally identifie...

journal_title：Archives of biochemistry and biophysics

authors： Whittaker MM,Penmatsa A,Whittaker JW

更新日期：2015-02-15 00:00:00

abstract：:When applied in liquid paraffin to the upper surface of expanding spinach leaves, [1-14C]palmitic acid was efficiently and exclusively incorporated into the sn-1 position of cellular glycerolipids, principally phosphatidylcholine and triacylglycerol. A slow transfer of fatty acids from phosphatidylcholine to chloropla...

journal_title：Archives of biochemistry and biophysics

authors： Roughan PG,Thompson GA Jr,Cho SH

更新日期：1987-12-01 00:00:00

abstract：:Carcinoembryonic antigen-related cell adhesion molecules (CEACAMs) belong to the immunoglobulin superfamily and contribute to cell-cell adhesion and signal modulation in various tissues. In humans, several CEACAMs are targeted by pathogenic bacteria. One peculiar member of this family, CEACAM3, is exclusively expresse...

journal_title：Archives of biochemistry and biophysics

authors： Buntru A,Roth A,Nyffenegger-Jann NJ,Hauck CR

更新日期：2012-08-01 00:00:00

abstract：:Ca(2+)-loaded rat liver mitochondria treated with 3,5,3'-triiodothyronine (T3) undergo nonspecific inner membrane permeabilization, as evidenced by mitochondrial swelling, a decrease in membrane potential (delta psi), and an increase in the rate of oxygen uptake. T3 analogues thyroxine (T4), 3',5'-diiodothyronine (T2)...

journal_title：Archives of biochemistry and biophysics

authors： Castilho RF,Kowaltowski AJ,Vercesi AE

更新日期：1998-06-01 00:00:00

abstract：:The glucose transporter of the bacterial phosphotransferase system mediates sugar transport across the cytoplasmic membrane concomitant with sugar phosphorylation. It consists of a cytoplasmic subunit IIA(Glc) and the transmembrane subunit IICB(Glc). IICB(Glc) was purified to homogeneity by urea/alkali washing of memb...

journal_title：Archives of biochemistry and biophysics

authors： Zhuang J,Gutknecht R,Flükiger K,Hasler L,Erni B,Engel A

更新日期：1999-12-01 00:00:00

abstract：:Activation of the delta-isoform of protein kinase C (deltaPKC) by certain conditions of oxidative stress results in translocation of the kinase to the mitochondria leading to release of cytochrome c and the induction of apoptosis. In the current study, the effects of myocardial reperfusion-induced deltaPKC translocati...

journal_title：Archives of biochemistry and biophysics

authors： Churchill EN,Szweda LI

更新日期：2005-07-15 00:00:00

abstract：:The effects of temperature on the initial velocity kinetics of allosteric ATP sulfurylase from Penicillium chrysogenum were measured. The experiments were prompted by the structural similarity between the C-terminal regulatory domain of fungal ATP sulfurylase and fungal APS kinase, a homodimer that undergoes a tempera...

journal_title：Archives of biochemistry and biophysics

authors： Medina DC,Hanna E,MacRae IJ,Fisher AJ,Segel IH

更新日期：2001-09-01 00:00:00

abstract：:The plant-derived flavonoid, quercetin (QCT), has many biological actions, including cardioprotective actions, resulting from its antioxidant and anti-inflammatory effects. In this study, effects of QCT and its metabolites on the contraction and Ca2+ transients (CaT) of mouse single cardiomyocytes were simultaneously ...

journal_title：Archives of biochemistry and biophysics

authors： Hayamizu K,Morimoto S,Nonaka M,Hoka S,Sasaguri T

更新日期：2018-01-01 00:00:00

abstract：:Prostaglandin endoperoxide H synthases-1 and -2 (PGHS-1 and -2) are integral membrane proteins associated with the luminal surface of the endoplasmic reticulum (ER) and the nuclear envelope (NE). The C-terminal sequences of PGHSs end in -Ser/Pro-Thr-Glu-Leu (-S/PTEL), a sequence similar to one of the known ER targetin...

journal_title：Archives of biochemistry and biophysics

authors： Song I,Smith WL

更新日期：1996-10-01 00:00:00

abstract：:We have cloned and overexpressed a variant of Arabidopsis thaliana beta-carbonic anhydrase (Q158A) that deletes the functional equivalent of the backbone amide NH of Thr-199 in human alpha-carbonic anhydrase II. The latter residue is hypothesized to be important in catalyzing the rate of CO(2)(-) HCO (3)(-) interconve...

journal_title：Archives of biochemistry and biophysics

authors： Rowlett RS,Tu C,Murray PS,Chamberlin JE

更新日期：2004-05-01 00:00:00

abstract：:A new plant lectin from elderberry (Sambucus nigra L.) bark, which was shown by immunochemical techniques to bind specifically to terminal Neu5Ac(alpha 2-6)Gal/GalNAc residues of glycoconjugates, was immobilized onto Sepharose 4B (SNA-Sepharose) and its carbohydrate binding properties was determined using a series of ...

journal_title：Archives of biochemistry and biophysics

authors： Shibuya N,Goldstein IJ,Broekaert WF,Nsimba-Lubaki M,Peeters B,Peumans WJ

更新日期：1987-04-01 00:00:00

abstract：:Studies have followed the turbidity (OD400 nm) of beta-casein (CN) as temperature (T) increased from 4 to 37 degrees C. Native non-phosphorylated beta-CN showed a turbidity increase above 25 degrees C and precipitated at about 22 degrees C in 5mM Ca+2. These patterns were reproducible upon T-cycling while those of rec...

journal_title：Archives of biochemistry and biophysics

authors： Sood SM,Booth C,Jhawar H,Slattery CW

更新日期：2006-10-01 00:00:00

abstract：:The ability of chelators and ascorbic acid to mobilize iron from crocidolite, amosite, medium- and short-fiber chrysotile, and tremolite was investigated. Ferrozine, a strong Fe(II) chelator, mobilized Fe(II) from crocidolite (6.6 nmol/mg asbestos/h) and amosite (0.4 nmol/mg/h) in 50 mM NaCl, pH 7.5. Inclusion of asco...

journal_title：Archives of biochemistry and biophysics

authors： Lund LG,Aust AE

更新日期：1990-04-01 00:00:00