Abstract：
:The CD46 lymphocyte surface antigen of man (until recently called HuLy-m5), and defined by the E4.3 monoclonal antibody (MoAb), shares cross-reactive antigenic epitopes with the envelope gp70 glycoproteins of gibbon ape leukaemia virus (GaLV) and Mason Pfizer monkey virus (MPMV) primate retroviruses. It is now shown that the cross-reactive antigenic epitope shared by these three molecules is determined solely by the protein portion of these glycoproteins, and that the N-linked and O-linked carbohydrate moieties of these glycoproteins do not directly or sterically contribute to the antigenic cross-reactivity. When CD46 molecules (mol.wt = 66 and 56 kDa) from human thymocytes were stripped of sialic acid with neuraminidase, or stripped of N-linked carbohydrate with endoglycosidase F, the E4.3 MoAb was still able to bind and immunoprecipitate the protein core of CD46 (mol.wt = 56 and 44 kDa). Similarly, polyclonal antisera to GaLV and MPMV precipitated deglycosylated CD46, although at a reduced efficiency. The cross-reacting E4.3 MoAb, anti-GaLV and anti-MPMV antisera also immunoprecipitated HuLy-m5 primary translation protein lacking N- or O-linked carbohydrate from the in vitro translation products of human thymocyte mRNA. Thus, the antigenic cross-reactivity of CD46 molecules with GaLV gp70 and MPMV gp70 is both specific and due to protein structure rather than to carbohydrate; the findings suggest that retroviruses may have acquired a functional epitope from human CD46 or that an endogenous retroviral sequence of human may partially or completely encode the CD46 antigen.
journal_name
Immunol Cell Bioljournal_title
Immunology and cell biologyauthors
Purcell DF,Deacon NJ,McKenzie IFdoi
10.1038/icb.1989.42subject
Has Abstractpub_date
1989-10-01 00:00:00pages
279-89eissn
0818-9641issn
1440-1711journal_volume
67 ( Pt 5)pub_type
杂志文章abstract：:Asthma is a common and complex inflammatory disease of the airways that remains incurable. Current forms of therapy are long term and may exhibit associated side-effect problems. Major participants in the development of an asthma phenotype include the triggering stimuli such as the allergens themselves, cells such as ...
journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
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journal_title：Immunology and cell biology
pub_type： 杂志文章
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journal_title：Immunology and cell biology
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journal_title：Immunology and cell biology
pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1992.22
更新日期：1992-06-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章
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更新日期：1995-04-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章
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更新日期：2005-06-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章
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更新日期：2017-01-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1990.22
更新日期：1990-06-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
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更新日期：1998-06-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
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更新日期：2014-03-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1997.95
更新日期：1997-12-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
doi：10.1038/sj.icb.7100006
更新日期：2007-01-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章
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更新日期：2001-06-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章
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更新日期：2013-02-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章
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更新日期：2007-04-01 00:00:00
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journal_title：Immunology and cell biology
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更新日期：2017-02-01 00:00:00
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更新日期：1998-04-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1046/j.1440-1711.2002.01052.x
更新日期：2002-02-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章
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更新日期：1997-02-01 00:00:00
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更新日期：2015-01-01 00:00:00
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更新日期：2017-02-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1038/icb.1990.15
更新日期：1990-04-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章
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更新日期：1991-06-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章,评审
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更新日期：2000-08-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章
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更新日期：2010-08-01 00:00:00
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journal_title：Immunology and cell biology
pub_type： 杂志文章
doi：10.1111/j.1440-1711.2006.01460.x
更新日期：2006-10-01 00:00:00
