Abstract:
AIMS:The present study was to investigate the effect of hydrogen peroxide (H2O2) on bone marrow stem cells and their endothelial differentiation and the underlying mechanisms in vitro. MAIN METHODS:Rat bone marrow multipotent adult progenitor cells (MAPCs) were used as the source of bone marrow stem cells, and treated with H2O2 (with the final concentration from 0 to 50 μM) with or without N-acetylcysteine (NAC, 0.1 mM). Reactive oxygen species (ROS) was measured by electron paramagnetic resonance (EPR) and fluorescent microscope. Flow cytometry and immunoblotting were used to determine apoptosis and differentiation of MAPCs. KEY FINDINGS:H2O2 generated a significant amount of intracellular and extracellular ROS in the culture system, substantially inhibited the proliferation of MAPCs and Oct-4 expression, and induced their apoptosis in a dose-dependent manner. Exposure to H2O2 also significantly attenuated the endothelial differentiation of MAPCs with reduced expression of endothelial markers CD31 and FLK-1 as well as impaired in vitro vascular structure formation. Both intracellular and extracellular ROS production from H2O2 were blocked by NAC. NAC treatment effectively prevented H2O2-induced reduction of Oct-4 expression in the cells. However, NAC treatment only partially prevented H2O2-induced apoptosis, and inhibition of cell proliferation and endothelial differentiation of MAPCs. SIGNIFICANCE:H2O2 exposure suppressed Oct-4 expression in MAPCs through ROS-dependent mechanism, while increasing the apoptosis of MAPCs and inhibiting their proliferation and endothelial differentiation with a mechanism partially due to ROS generation in vitro.
journal_name
Life Scijournal_title
Life sciencesauthors
Xiao Y,Li X,Cui Y,Zhang J,Liu L,Xie X,Hao H,He G,Kander MC,Chen M,Liu Z,Verfaillie CM,Zhu H,Lei M,Liu Zdoi
10.1016/j.lfs.2014.07.016subject
Has Abstractpub_date
2014-09-01 00:00:00pages
33-40issue
1-2eissn
0024-3205issn
1879-0631pii
S0024-3205(14)00625-0journal_volume
112pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract::The content of lactic acid in red blood cells, plasma, and Ehrlich ascites tumor cells were measured by a gas-liquid chromatography using a column with a terephtalic acid support coated with polyethylene glycol-6000. The lactic acid contents were directly determined in aqueous samples, because they were converted to a...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(92)90407-g
更新日期:1992-01-01 00:00:00
abstract::Abnormal activation of the beta-catenin signaling pathway can cause various types of cancer. Activation of Wnt pathway leads to stabilization of the beta-catenin protein, which results in its translocation to the nucleus and the formation of complexes with lymphoid enhancer factor-1 (LEF-1) and other T-cell factor (TC...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2005.02.009
更新日期:2005-07-29 00:00:00
abstract::The effect of the Type I benzodiazepine (BDZ) receptor agonist, CL 218,872, on convulsions generated by low doses of methyl beta-carboline-3-carboxylic acid ( MBCC ), bicuculline, picrotoxin and pentylenetetrazole (PTZ) in mice was examined. Low doses of CL 218,872 enhanced the convulsions produced by all agents excep...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(84)90321-7
更新日期:1984-05-28 00:00:00
abstract::This study was designed to evaluate the effects of roxithromycin (RXM), a newly synthesized macrolide antibiotic on allergic responses in mice. RXM was orally administered into BALB/c mice once a day for 42 days in a single dose of 5 mg/kg body weight. Spleen cells (Sp C) collected from mice on day 7, 14, 28 and 42 po...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(93)90154-u
更新日期:1993-01-01 00:00:00
abstract::Interactions of the synthetic pyrethroid allethrin with the nicotinic acetylcholine (ACh) receptor/channel were studied in membranes from Torpedo electric organ. Allethrin did not inhibit binding of [3H]ACh to the receptor sites, but inhibited noncompetitively binding of [3H]perhydrohistrionicotoxin ([3H]H12-HTX) to t...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(82)90045-5
更新日期:1982-10-11 00:00:00
abstract::Ischemic preconditioning (IP) may protect the lung from ischemia-reperfusion (I/R) injury following cardiopulmonary by-pass and lung or heart transplantation. The present study was undertaken to investigate the role of ATP-dependent potassium channels (K(ATP)) in IP in the isolated buffer-perfused rat lung (IBPR) unde...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2006.07.011
更新日期:2006-11-02 00:00:00
abstract:AIMS:Salivary gland dysfunction is a common complication of diabetes mellitus (DM). Long non-coding RNA (lncRNA) is evidenced to involve in the functional regulation of salivary gland, however, its role in DM-impaired gland is unknown. Therefore, this study aimed to investigate the expression profiles and functional ne...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2020.119009
更新日期:2021-01-04 00:00:00
abstract::It was examined whether the digoxin-like immunoreactive substance (DLIS) extracted from cord blood has a natriuretic activity. The DLIS was prepared from cord blood of healthy fullterm infants by acetone-HCl extraction and a gel filtration column. A solution (solution A) containing 1.0 ng/ml of DLIS or another solutio...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(88)90639-x
更新日期:1988-01-01 00:00:00
abstract::We studied the effect of thapsigargin on intracellular calcium levels ([Ca2+]i) measured by fura-2 fluorimetry in Madin Darby canine kidney (MDCK) cells. Thapsigargin elevated [Ca2+]i dose dependently with an EC50 of approximately 0.15 microM. The Ca2+ signal consisted of a slow rise, a gradual decay and a plateau. De...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(98)00561-x
更新日期:1999-01-01 00:00:00
abstract::The temporal relationship of changes in cAMP and cGMP to oocyte maturation was examined in proestrous hamsters (day 4). The first series of experiments showed, in normal cycling hamsters, an increase in cAMP and a decrease in cGMP at 1400 h shortly after the rise in LH with oocyte maturation beginning at 1800 h. When ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90726-9
更新日期:1983-10-24 00:00:00
abstract::An increased level of melanin is characteristic of a large number of skin diseases, including acquired hyperpigmentation conditions such as melasma, post inflammatory melanoderma, and solar lentigo. Thus, there is an increasing need for the development of depigmenting agents. In order to evaluate the depigmenting capa...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2007.05.009
更新日期:2007-06-27 00:00:00
abstract::Clinical reports suggest that EGFR-mutated lung cancer usually respond significantly towards small molecule tyrosine kinase inhibitors. Same studies also report the eventual development of acquired resistance within a median time interval of 9 to 14months. One of the major mechanisms involved in this acquired resistan...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2017.08.001
更新日期:2017-10-01 00:00:00
abstract::Hyaluronan (HA) is a multifunctional high molecular weight polysaccharide found throughout the animal kingdom, especially in the extracellular matrix (ECM) of soft connective tissues. HA is thought to participate in many biological processes, and its level is markedly elevated during embryogenesis, cell migration, wou...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/j.lfs.2007.02.037
更新日期:2007-05-01 00:00:00
abstract::Using a specific radioimmunoassay, we measured concentrations of plasma 7B2 (a novel pituitary polypeptide) immunoreactivity (7B2-IR) in normal human subjects, patients with chronic renal failure and those with liver cirrhosis. Mean (+/- SEM) values of plasma 7B2-IR in normal healthy men and women were 55.8 +/- 1.2 pg...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90685-0
更新日期:1987-08-24 00:00:00
abstract::The development of the specific uptake of dopamine in the rat striatum during the early postnatal period is compared with the ontogenetic changes of the specific binding of (3H)GBR 12783 to the site of uptake inhibition. During maturation, the increase in the specific binding of (3H)GBR 12783 parallels the increase in...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(89)90563-8
更新日期:1989-01-01 00:00:00
abstract::An animal model of haloperidol-induced tardive dyskinesia was studied in relation to the dietary manipulation of tryptophan and its effect on the movement disorder. This study showed a significant negative behavioral response to the neuroleptic drug, haloperidol. Increased dietary tryptophan (1.0 vs. 0.3%) significant...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(87)90602-3
更新日期:1987-10-05 00:00:00
abstract:AIMS:This study aims to develop and evaluate oleuropein loaded surface functionalized folate-targeted - PEG liposomes for the effective management of prostate cancer in an animal model. MATERIALS AND METHODS:Film hydration-cum-extrusion technique was used to produce liposomes. Particle size, entrapment efficiency, dru...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2019.01.053
更新日期:2019-03-01 00:00:00
abstract::Ventricular hypertrophy is a risk factors for arrhythmias, ischemia and sudden death. It involves cellular modifications leading to a pathological remodeling and is associated with heart failure. The activation of the G protein-coupled estrogen receptor (GPER) mediates beneficial actions in the cardiovascular system. ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2019.117211
更新日期:2020-02-01 00:00:00
abstract:AIMS:In this study, we investigated the effects of Bax-inhibiting peptide (Bip)-V5, an anti-apoptosis membrane-permeable peptide, on the 6-hydroxydopamine (6-OHDA) induced Parkinson's disease (PD) model rats. MATERIALS AND METHODS:Rats were randomly divided into five groups: Control, 6-OHDA only, Vehicle+6-OHDA, zVAD+...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2015.11.019
更新日期:2016-01-01 00:00:00
abstract::The rapid progress in the past few years concerning neurotrophic factor research, has greatly stimulated advances in developmental neurobiology of hearing. We have summarized evidence that neurotrophins are expressed by auditory sensory epithelia during the time at which ganglion cells with neurotrophin receptors send...
journal_title:Life sciences
pub_type: 杂志文章,评审
doi:10.1016/0024-3205(94)00506-0
更新日期:1994-01-01 00:00:00
abstract::Propentofylline (PPF) is a xanthine derivative with pharmacological effects that are distinct from those of classic methylxanthines. It depresses the activation of microglial cells and astrocytes, which is associated with neuronal damage during neural inflammation and hypoxia. Our previous studies showed that PPF impr...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2016.02.028
更新日期:2016-03-01 00:00:00
abstract:AIMS:The rational basis that explains the benefits of exercise therapy on Chagas cardiomyopathy (ChC) is poorly understood. This study investigated the impact of an exercise program on exercise performance, heart parasitism, immunoinflammatory response, fibrogenesis, oxidative damage, and cardiomyocytes contractility i...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2016.03.053
更新日期:2016-05-01 00:00:00
abstract::Neuropathic pain syndromes respond poorly to opioid treatment. In our previous studies, we found that intrathecal (i.t.) injection of pertussis toxin (PTX) produces thermal hyperalgesia, which is poorly responsive to morphine and is accompanied by an increase in cerebrospinal fluid (CSF) levels of excitatory amino aci...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2005.08.020
更新日期:2006-03-13 00:00:00
abstract::Ocimum sanctum, the Indian holy basil, has significant ability to scavenge highly reactive free radicals. Shade dried leaf powder of the plant was extracted with water and alcohol, and then fractionated with different solvents. Both extracts and their fractions have in vitro anti-lipidperoxidative activity at very low...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2004.05.036
更新日期:2004-11-19 00:00:00
abstract::In the course of investigating the binding of imipramine to soluble cellular fractions from brain and leukocytes using equilibrium dialysis, an artifactual binding component was produced. On Scatchard and double-reciprocal plots of the data, the component appeared as a homogenous population of sites displaying a bindi...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/0024-3205(83)90405-8
更新日期:1983-07-11 00:00:00
abstract::Recently, 4.4'-bis(1-p-carboxyphenyl-3-methyl-5-dydroxyl)-pyrazol (DRD156) has been developed as a new sensitive reagent that reacts specifically with singlet oxygen. The specificity of DRD156 for singlet oxygen in a biomimetic solution (micellar solution) and the effects of its coexistence with other reagent were exa...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(02)02234-8
更新日期:2002-12-06 00:00:00
abstract::Endomorphin 1 (EM1) and endomorphin 2 (EM2) are highly potent and selective mu-opioid receptor agonists and have significant antinociceptive action. In the mu-selective pocket of endomorphins (EMs), Pro2 residue is a spacer and directs the Tyr1 and Trp3/Phe3 side chains into the required orientation. The present work ...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2005.03.005
更新日期:2005-07-22 00:00:00
abstract:AIMS:Endothelial dysfunction is a key triggering event in the development of cardiovascular diseases and the current study explored this phenomenon in the context of inflammation, apoptosis, reactive oxygen species (ROS) and the phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway during chronic diabetes. MAIN M...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2010.03.019
更新日期:2010-06-05 00:00:00
abstract:OBJECTIVE:The aim of this study was to investigate the role of HLA-DR, HLA-G and CD99 during cervical carcinogenesis and to examine the prognostic significance of these protein expressions in invasive squamous cell carcinoma (SCC). METHODS:Using specific antibodies for HLA-DR, HLA-G and CD99, we examined protein expre...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/j.lfs.2005.10.039
更新日期:2006-04-25 00:00:00
abstract::Cumulative damage in cells from aged people could lead to a greater fragility against acute oxidative stress. The effects of acute oxidative stress on cell viability, cAMP and cGMP concentrations, and protein synthesis rates were studied in lymphocytes from 25 young and 26 elderly subjects. Lymphocytes were exposed to...
journal_title:Life sciences
pub_type: 杂志文章
doi:10.1016/s0024-3205(98)00231-8
更新日期:1998-01-01 00:00:00