Abstract:
:Therapeutic drug monitoring of antipsychotics is important in optimizing individual therapy. In psychiatric populations, classical venous blood sampling is experienced as frightening. Interest in alternative techniques, like dried blood spots (DBS), has consequently increased. A fast and easy to perform DBS method for quantification of 16 antipsychotics (amisulpride, aripiprazole, asenapine, bromperidol, clozapine, haloperidol, iloperidone, levosulpiride, lurasidone, olanzapine, paliperidone, pipamperone, quetiapine, risperidone, sertindole and zuclopenthixol) and 8 metabolites was developed. DBS were prepared using 25 μL of whole blood and extraction of complete spots was performed using methanol: methyl-t-butyl-ether (4:1). After evaporation, the extract was reconstituted in the mobile phase and 10 μL were injected on an ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Separation using a C18 column and gradient elution with a flow rate of 0.5 mL/min resulted in a 6-min run-time. Ionization was performed in positive mode and a dynamic MRM method was applied. Median recovery was 66.4 % (range 28.7-84.5%). Accuracy was within the acceptance criteria, except for pipamperone (LLOQ and low concentration) and lurasidone (low concentration). Imprecision was only aberrant for lurasidone at low and medium concentration. All compounds were stable during 1 month at room temperature, 4 °C and -18 °C. Lurasidone was unstable when the extract was stored for 12 h on the autosampler. Absolute matrix effects (ME) (median 66.1%) were compensated by the use of deuterated IS (median 98.8%). The DBS method was successfully applied on 25-μL capillary DBS from patients and proved to be a reliable alternative for quantification of all antipsychotics except for olanzapine and N-desmethylolanzapine.
journal_name
Drug Test Analjournal_title
Drug testing and analysisauthors
Patteet L,Maudens KE,Stove CP,Lambert WE,Morrens M,Sabbe B,Neels Hdoi
10.1002/dta.1698subject
Has Abstractpub_date
2015-06-01 00:00:00pages
502-11issue
6eissn
1942-7603issn
1942-7611journal_volume
7pub_type
杂志文章abstract::Selective androgen receptor modulators (SARMs) are a group of anabolic enhancer drugs posing threats to the integrity of animal sports and the safety of animal-derived foods. The current research describes for the first time the development of a semi-quantitative assay for the monitoring of SARM family compounds in bl...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.2875
更新日期:2020-09-01 00:00:00
abstract::We recently published two protocols for the detection of Sotatercept (ACE-011, ACVR2A-Fc) and Luspatercept (ACE-536, ACVR2B-Fc) in human serum. Both methods used covalently immobilized antibodies on agarose beads for immunoprecipitation and SAR-PAGE/Western blotting for detection. Disadvantages were the relatively hig...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.2500
更新日期:2018-11-01 00:00:00
abstract::The method of high sensitive gas chromatographic/time-of-flight mass-spectrometric (GC/TOF-MS) analysis of steroids was developed. Low-resolution TOF-MS instrument (with fast spectral acquisition rate) was used. This method is based on the formation of the silyl derivatives of steroids; exchange of the reagent mixture...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.221
更新日期:2011-04-01 00:00:00
abstract::Evidence of the continuous rise of novel doping agents and novel doping strategies calls for the development of more accurate multi-target screening methods. Direct multi-target screening approaches are restricted to the targeted substances and their turnover. The development of effective "indirect" screening methods ...
journal_title:Drug testing and analysis
pub_type: 杂志文章,评审
doi:10.1002/dta.2788
更新日期:2020-06-01 00:00:00
abstract::Relaxin (RLX) has demonstrated diverse pharmacological effects in various scientific and clinical studies. The emergence of porcine relaxin (pRLX) has raised concerns on the doping potential of pRLX. There have also been speculations in the horseracing industry on its covert use. To control the abuse of pRLX in equine...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.2143
更新日期:2017-09-01 00:00:00
abstract::Detection of the abuse of synthetic steroids in food production is nowadays relatively straightforward using modern techniques such as gas or liquid chromatography coupled to mass spectrometry (GC-MS/MS or LC-MS/MS, respectively). However, proving the abuse of 'endogenous' (or naturally occurring) steroids is more dif...
journal_title:Drug testing and analysis
pub_type: 杂志文章,评审
doi:10.1002/dta.1354
更新日期:2012-08-01 00:00:00
abstract::Tretoquinol (trimetoquinol), a β2-agonist, has been explicitly listed on the World Anti-Doping Agency Prohibited List 2019 since January 2019; however, it has been distributed as an antiasthmatic on the medical market. This study aimed to develop a liquid chromatography-tandem mass spectrometric method for the quantif...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.2714
更新日期:2019-11-01 00:00:00
abstract::Accurate on-site devices to screen for drug intake are critical for establishing whether an individual is driving under the influence of drugs (DUID); however, on-site oral fluid (OF) cocaine device performance is variable. We evaluated the performance of a newly developed benzoylecgonine (BE) test-strip for the Draeg...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.1966
更新日期:2016-03-01 00:00:00
abstract::The effectiveness of doping control in sport has been improved continuously during the past 50 years. One of the major steps forward was the introduction of unannounced and targeted out-of-competition testing in order to control the misuse of anabolic-androgenic steroids (AAS), mainly during the end of the 1980s. It a...
journal_title:Drug testing and analysis
pub_type: 信件
doi:10.1002/dta.2727
更新日期:2020-05-01 00:00:00
abstract::Δ(9) -Tetrahydrocannabinol (THC) is the most commonly analyzed cannabinoid in oral fluid (OF); however, its metabolite 11-nor-9-carboxy-THC (THCCOOH) offers the advantage of documenting active consumption, as it is not detected in cannabis smoke. Analytical challenges such as low (ng/L) THCCOOH OF concentrations hampe...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.1753
更新日期:2015-08-01 00:00:00
abstract::Current doping trends overshadow the traditional interpretation of using prohibited performance-enhancing drugs to improve training and/or sports results. Its definition has evolved due to several recent cases of unconventional manipulation in sports. In this article, we present a case study of an intentional intoxica...
journal_title:Drug testing and analysis
pub_type: 历史文章,杂志文章
doi:10.1002/dta.2753
更新日期:2020-05-01 00:00:00
abstract::Urine samples were analyzed for lactose to investigate if elevated lactose concentrations indicate recent (< 48 hours) intravenous abuse of substances containing lactose as an excipient. Elevated lactose levels were found in samples given by patients who had recently injected substances intravenously, verified by fres...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.2654
更新日期:2019-09-01 00:00:00
abstract::MSforID represents a database of tandem mass spectral data obtained from (quasi-)molecular ions produced by atmospheric pressure ionization methods. At the current stage of development the library contains 12 122 spectra of 1208 small (bio-)organic molecules. The present work was aimed to evaluate the performance of t...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.341
更新日期:2012-03-01 00:00:00
abstract::1-Propanoyl-lysergic acid diethylamide (1P-LSD) appeared as a non-controlled alternative to LSD a few years ago. Although evidence is beginning to emerge from in vitro and animal studies that 1P-LSD might serve as a prodrug for LSD, an equivalent evaluation in humans is unavailable. Controlled oral and intravenous sel...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.2821
更新日期:2020-08-01 00:00:00
abstract::Fast and unequivocal drug detection is of considerable importance in numerous fields of analytical chemistry, and today mass spectrometry-based approaches are often the method of choice due to their sensitive and specific nature. Mass spectrometry is in constant flux with innovations and thus supports the development ...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.344
更新日期:2012-03-01 00:00:00
abstract::Metal ion coordination ionspray (M(+) CIS) ionization is a powerful technique to enhance ionization efficiency and sensitivity. In this study, we developed and validated an analytical method for simultaneous ionization and analysis of 84 anabolic androgenic steroids (65 exogenous and 19 endogenous) using liquid chroma...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.1747
更新日期:2014-11-01 00:00:00
abstract::The legally defensible proof of the abuse of endogenous steroids in sports is currently based on carbon isotope ratio mass spectrometry (IRMS), i.e. a comparison between (13)C/(12)C ratios of diagnostic precursors and metabolites of testosterone. The application of this technique requires a chromatographic baseline se...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.1536
更新日期:2013-11-01 00:00:00
abstract::The evolving nature of new psychoactive substances (NPS) - often referred to as 'legal highs', 'designer drugs' or 'bath salts' - presents an evolving challenge for toxicologists. Apart from the detection and identification of these compounds, further analytical challenges may arise from the presence of possible metab...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.1576
更新日期:2014-07-01 00:00:00
abstract::In Italy, Workplace Drug Testing (WDT) has been compulsory by law for specific categories of workers since 2008, offering the opportunity to compare studies conducted within a single regulatory framework. The aims of this paper are to estimate the overall prevalence of WDT positivity (at screening survey) among Italia...
journal_title:Drug testing and analysis
pub_type: 杂志文章,meta分析,评审
doi:10.1002/dta.2189
更新日期:2017-06-01 00:00:00
abstract::Trimethoprim (TMP) is a bacteriostatic antibiotic mainly used in the prophylaxis and treatment of urinary tract infections. It belongs to the class of chemotherapeutic agents known as dihydrofolate reductase inhibitors. Its use is associated with idiosyncratic reactions, including liver toxicity and agranulocytosis. I...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.161
更新日期:2010-09-01 00:00:00
abstract::Three sensitive, selective and precise stability-indicating methods for the determination of the anti-Alzheimer's drug, rivastigmine hydrogen tartrate (RIV) in the presence of its alkaline degradation product (major metabolite, NAP 226-90) and in pharmaceutical formulation were developed and validated. The first metho...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.121
更新日期:2010-05-01 00:00:00
abstract::Vaping of synthetic cannabinoids via e-cigarettes is growing in popularity. In the present study, we tentatively identified 12 by-products found in a pure sample of the synthetic cannabinoid Cumyl-5F-PINACA (1-(5-fluoropentyl)-N-(2-phenylpropan-2-yl)-1H-indazole-3-carboxamide), a prevalent new psychoactive substance (...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.2673
更新日期:2020-01-01 00:00:00
abstract::According to the World Anti-Doping Agency (WADA) technical document for erythropoiesis stimulating agents (ESA) analysis (TD2014EPO), double-blotting of serum/plasma samples is mandatory for all analysis by isoelectric focusing (IEF) and for the confirmation procedures (CP) performed by SDS-PAGE or SAR-PAGE. The goal ...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.2320
更新日期:2017-11-01 00:00:00
abstract::Anabolic-androgenic steroids are synthetic compounds prohibited due to their performance-enhancing characteristics. The use of these substances is known to cause health-related issues, which highlights the importance of being able to evaluate the scale of consumption by the general population. However, most available ...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.2591
更新日期:2019-07-01 00:00:00
abstract::The accuracy in predicting different chemometric methods was compared when applied on ordinary UV spectra and first order derivative spectra. Principal component regression (PCR) and partial least squares with one dependent variable (PLS1) and two dependent variables (PLS2) were applied on spectral data of pharmaceuti...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.235
更新日期:2011-05-01 00:00:00
abstract::Erythropoietin (EPO) has been misused in sports for many years due to its performance-enhancing effect. In the last decade, detection of abuse has been possible with isoelectric focusing (IEF) based on the different isoform profiles of endogenous and recombinant EPO. The release of new EPOs on the market, such as the ...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.270
更新日期:2011-05-01 00:00:00
abstract::Higenamine was included in the World Anti-Doping Agency (WADA) Prohibited Substances and Methods List as a β2 -adrenoceptor agonist in 2017, thereby resulting in its prohibition both in and out of competition. The present mini review describes the physiology and pharmacology of adrenoceptors, summarizes the literature...
journal_title:Drug testing and analysis
pub_type: 杂志文章,评审
doi:10.1002/dta.2992
更新日期:2020-12-26 00:00:00
abstract::The impact of altitude training on haematological parameters and the Athlete Biological Passport (ABP) was evaluated in international-level elite athletes. One group of swimmers lived high and trained high (LHTH, n = 10) for three to four weeks at 2130 m or higher whereas a control group (n = 10) completed a three-wee...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.1757
更新日期:2015-08-01 00:00:00
abstract::Anabolic steroids are banned substances in equine sports. Gas chromatography-mass spectrometry (GC-MS) has been the traditional technique for doping control analysis of anabolic steroids in biological samples. Although liquid chromatography-mass spectrometry (LC/MS) has become an important technique in doping control,...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.2090
更新日期:2017-09-01 00:00:00
abstract::Phosphatidylethanol 16:0/18:1 (PEth) is the most abundant homologue of the phosphatidylethanol group of phospholipids. Formed only in the presence of ethanol, PEth is used as a biomarker in whole blood to provide information about the consumption of alcohol. As information on the storage life of PEth is essential for ...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.2995
更新日期:2021-01-04 00:00:00