Effect of single doses of methoxypolyethylene glycol-epoetin beta (CERA, Mircera™) and epoetin delta (Dynepo™) on isoelectric erythropoietin profiles and haematological parameters.

Abstract:

:Erythropoietin (EPO) has been misused in sports for many years due to its performance-enhancing effect. In the last decade, detection of abuse has been possible with isoelectric focusing (IEF) based on the different isoform profiles of endogenous and recombinant EPO. The release of new EPOs on the market, such as the recombinant erythropoietin epoetin delta (Dynepo™) and the chemically modified EPO, CERA (Mircera™) potentially represents analytical challenges to the fight against doping. This study set out to investigate the possibility of and the time window for detecting the administration of a single dose of Dynepo™ and CERA. Our results are in agreement with earlier findings that detection of Dynepo™ is best achieved by combining IEF with SDS-PAGE. Haematological parameters were monitored for possible effects due to the long half-life (130 hours) of CERA in blood. Interestingly, although several haematological parameters were significantly changed after the injection of CERA, the endogenous EPO signal was still present in all collected samples. Due to the long half-life and the large size of the CERA molecule (about 60 kDa), it was uncertain whether CERA would be excreted into urine in detectable amounts unless urine collection was preceded by strenuous physical exercise. We find that CERA can be detected in urine without prior exercise in several, but not all, subjects. CERA is nevertheless best detected in serum with regard to both probability and length of detection, in addition to stability in matrix over time.

journal_name

Drug Test Anal

authors

Dehnes Y,Hemmersbach P

doi

10.1002/dta.270

subject

Has Abstract

pub_date

2011-05-01 00:00:00

pages

291-9

issue

5

eissn

1942-7603

issn

1942-7611

journal_volume

3

pub_type

杂志文章
  • Circulating microRNAs as long-term biomarkers for the detection of erythropoiesis-stimulating agent abuse.

    abstract::MicroRNAs (miRNAs) are small, non-protein coding transcripts involved in many cellular and physiological mechanisms. Recently, a new class of miRNA called 'circulating miRNAs' was found in cell-free body fluids such as plasma and urine. Circulating miRNAs have been shown to be very stable, specific, and sensitive biom...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.370

    authors: Leuenberger N,Jan N,Pradervand S,Robinson N,Saugy M

    更新日期:2011-11-01 00:00:00

  • Ophthalmic delivery of sparfloxacin from in situ gel formulation for treatment of experimentally induced bacterial keratitis.

    abstract::The objective of the present work was (1) to develop an in situ gelling ophthalmic delivery system by combining pluronic F127 and pluronic F68, with sparfloxacin; and (2) to examine the influence of incorporating a mucoadhesive polysaccharide such as sodium hyaluronate on the healing property due to bacterial keratiti...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.170

    authors: Nesseem DI

    更新日期:2011-02-01 00:00:00

  • Meconium drug testing reveals maternal misuse of medicinal opioids among addicted mothers.

    abstract::Meconium drug testing is a non-invasive method to detect prenatal drug exposure, which can cause severe problems for the infant, indicating the need for follow-up measures to ensure the welfare of the child. Meconium samples for drug testing were collected from 143 infants as part of routine clinical work among addict...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章,多中心研究

    doi:10.1002/dta.1459

    authors: Launiainen T,Nupponen I,Halmesmäki E,Ojanperä I

    更新日期:2013-07-01 00:00:00

  • Effect of morin on pharmacokinetics of piracetam in rats, in vitro enzyme kinetics and metabolic stability assay using rapid UPLC method.

    abstract::The aim of this study was to investigate the effect of Morin on the pharmacokinetics of Piracetam in rats, in vitro enzyme kinetics and metabolic stability (high throughput) studies using human liver microsomes in UPLC. For pharmacokinetics studies, male Wistar rats were pretreated with Morin (10 mg/kg) for one week a...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.1382

    authors: Sahu K,Shaharyar M,Siddiqui AA

    更新日期:2013-07-01 00:00:00

  • Characterization of in vitro generated metabolites of the selective androgen receptor modulators S-22 and S-23 and in vivo comparison to post-administration canine urine specimens.

    abstract::Selective androgen receptor modulators (SARMs) have great therapeutic potential in various diseases including cancer cachexia, sarcopenia, and osteoporosis, and the number of drug candidates has been growing over the last decade. The SARM drug candidates S-22 and S-23 belong to one of the most advanced groups of andro...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.211

    authors: Thevis M,Gerace E,Thomas A,Beuck S,Geyer H,Schlörer N,Kearbey JD,Dalton JT,Schänzer W

    更新日期:2010-11-01 00:00:00

  • Strategy for identification and characterization of small quantities of drug degradation products using LC and LC-MS: application to valsartan, a model drug.

    abstract::The present study demonstrates the applicability of a strategy involving use of liquid chromatography (LC) and liquid chromatography-mass spectrometry (LC-MS) techniques for the identification and characterization of minute quantities of degradation products, without their isolation from the reaction matrix in pure fo...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.116

    authors: Mehta S,Shah RP,Singh S

    更新日期:2010-02-01 00:00:00

  • IFDAT-International forum for drug and alcohol testing, 12-14 April 2010, Barcelona, Spain.

    abstract::The second programme of its kind globally, the highly successful, collaborative, productive and energizing IFDAT-International Forum for Drug and Alcohol Testing, was held in Barcelona, Spain, from 12-14 April. IFDAT was attended by over 100 delegates, conference sponsors and exhibitors from the international workplac...

    journal_title:Drug testing and analysis

    pub_type:

    doi:10.1002/dta.150

    authors: Kenney J D JE,Björklöv P

    更新日期:2011-03-01 00:00:00

  • Identification and characterization of urinary prenylamine metabolites by means of liquid chromatography-tandem mass spectrometry.

    abstract::Prenylamine is a vasodilator of phenylalkylamine structure and was used for the treatment of angina pectoris, until reports of undesirable effects including ventricular tachycardia led to a decreasing use of the drug in the 1980s. Metabolic N-dealkylation of orally ingested prenylamine can liberate amphetamine in huma...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.1388

    authors: Beuck S,Sigmund G,Koch A,Schänzer W,Pokrywka A,Kwiatkowska D,Thevis M

    更新日期:2012-09-01 00:00:00

  • Return of the lysergamides. Part VI: Analytical and behavioural characterization of 1-cyclopropanoyl-d-lysergic acid diethylamide (1CP-LSD).

    abstract::Lysergic acid diethylamide (LSD) is a prototypical serotonergic psychedelic drug and the subject of many clinical investigations. In recent years, a range of lysergamides has emerged with the production of some being inspired by the existing scientific literature. Others, for example various 1-acyl substituted lyserga...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.2789

    authors: Brandt SD,Kavanagh PV,Westphal F,Stratford A,Odland AU,Klein AK,Dowling G,Dempster NM,Wallach J,Passie T,Halberstadt AL

    更新日期:2020-06-01 00:00:00

  • Evaluation of the performance of a tandem mass spectral library with mass spectral data extracted from literature.

    abstract::MSforID represents a database of tandem mass spectral data obtained from (quasi-)molecular ions produced by atmospheric pressure ionization methods. At the current stage of development the library contains 12 122 spectra of 1208 small (bio-)organic molecules. The present work was aimed to evaluate the performance of t...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.341

    authors: Würtinger P,Oberacher H

    更新日期:2012-03-01 00:00:00

  • Voltammetric determination of promethazine hydrochloride at a multi-wall carbon nanotube modified glassy carbon electrode.

    abstract::A simple and sensitive method is described for voltammetric determination of promethazine hydrochloride (PMZ), a widely used phenothiazine drug, based on its electrochemical oxidation at a multi-wall carbon nanotube (MWCNT) modified glassy carbon electrode (GCE). Compared with bare GCE, the MWCNT-modified GCE exhibite...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.205

    authors: Xi X,Ming L,Liu J

    更新日期:2011-03-01 00:00:00

  • Metabolites of lorazepam: Relevance of past findings to present day use of LC-MS/MS in analytical toxicology.

    abstract::The advent of liquid chromatography-tandem mass spectrometry (LC-MS/MS), with the sensitivity it confers, permits the analysis of both phase I and II drug metabolites that in the past would have been difficult to target using other techniques. These metabolites may have relevance to current analytical toxicology emplo...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.305

    authors: Turfus SC,Braithwaite RA,Cowan DA,Parkin MC,Smith NW,Kicman AT

    更新日期:2011-10-01 00:00:00

  • Drug-drug interaction and doping, part 2: an in vitro study on the effect of non-prohibited drugs on the phase I metabolic profile of stanozolol.

    abstract::The present study was designed to provide preliminary information on the potential impact of metabolic drug-drug interaction on the effectiveness of doping control strategies currently followed by the anti-doping laboratories to detect the intake of prohibited agents. In vitro assays based on the use of human liver mi...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.1608

    authors: Mazzarino M,de la Torre X,Fiacco I,Botrè F

    更新日期:2014-10-01 00:00:00

  • Spectrophotometric and spectrodensitometric methods for the determination of rivastigmine hydrogen tartrate in presence of its degradation product.

    abstract::Three sensitive, selective and precise stability-indicating methods for the determination of the anti-Alzheimer's drug, rivastigmine hydrogen tartrate (RIV) in the presence of its alkaline degradation product (major metabolite, NAP 226-90) and in pharmaceutical formulation were developed and validated. The first metho...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.121

    authors: Salem MY,El-Kosasy AM,El-Bardicy MG,Abd El-Rahman MK

    更新日期:2010-05-01 00:00:00

  • Annual banned-substance review: analytical approaches in human sports drug testing.

    abstract::The annual update of the list of prohibited substances and doping methods as issued by the World Anti-Doping Agency (WADA) allows the implementation of most recent considerations of performance manipulation and emerging therapeutics into human sports doping control programmes. The annual banned-substance review for hu...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章,评审

    doi:10.1002/dta.128

    authors: Thevis M,Kuuranne T,Geyer H,Schänzer W

    更新日期:2010-04-01 00:00:00

  • Seven years of workplace drug testing in Italy: A systematic review and meta-analysis.

    abstract::In Italy, Workplace Drug Testing (WDT) has been compulsory by law for specific categories of workers since 2008, offering the opportunity to compare studies conducted within a single regulatory framework. The aims of this paper are to estimate the overall prevalence of WDT positivity (at screening survey) among Italia...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章,meta分析,评审

    doi:10.1002/dta.2189

    authors: Rosso GL,Montomoli C,Morini L,Candura SM

    更新日期:2017-06-01 00:00:00

  • 1 H-15 N HMBC NMR as a tool for rapid identification of isomeric azaindoles: The case of 5F-MDMB-P7AICA.

    abstract::The high frequency of the synthetic cannabinoid receptor agonists (SCRAs) emergence renders this group of new psychoactive compounds particularly demanding in terms of detection, identification, and responding. Without the available reference material, one of the specific problems is differentiation and structure eluc...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.2573

    authors: Martek BA,Mihelač M,Gazvoda M,Virant M,Urankar D,Krivec M,Gostič T,Nemec B,Koštrun B,Janežič M,Klemenc S,Košmrlj J

    更新日期:2019-04-01 00:00:00

  • The use of in vitro technologies coupled with high resolution accurate mass LC-MS for studying drug metabolism in equine drug surveillance.

    abstract::The detection of drug abuse in horseracing often requires knowledge of drug metabolism, especially if urine is the matrix of choice. In this study, equine liver/lung microsomes/S9 tissue fractions were used to study the phase I metabolism of eight drugs of relevance to equine drug surveillance (acepromazine, azaperone...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.88

    authors: Scarth JP,Spencer HA,Timbers SE,Hudson SC,Hillyer LL

    更新日期:2010-01-01 00:00:00

  • GC-MS and GC-IRD studies on brominated dimethoxyamphetamines: regioisomers related to 4-Br-2,5-DMA (DOB).

    abstract::A series of regioisomeric bromodimethoxyamphetamines have mass spectra essentially equivalent to the controlled drug substance 4-Br-2,5-dimethoxyamphetamine (4-Br-2,5-DMA; DOB); all have molecular weight of 274 and major fragment ions in their electron ionization mass spectra at m/z 44 and m/z 230/232. The trifluoroac...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.409

    authors: Maher HM,Awad T,DeRuiter J,Randall Clark C

    更新日期:2012-07-01 00:00:00

  • Adulterants in selected dietary supplements and their detection methods.

    abstract::At present, there is a growing trend toward the intentional adulteration of dietary supplements (DS) with synthetic pharmaceuticals, which represents an alarming emerging risk to consumers and a serious problem for regulatory agencies. An amazing array of synthetic drugs and their analogues have been reported as adult...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章,评审

    doi:10.1002/dta.2806

    authors: Muschietti L,Redko F,Ulloa J

    更新日期:2020-07-01 00:00:00

  • Transcriptomic biomarkers of altered erythropoiesis to detect autologous blood transfusion.

    abstract::Autologous blood transfusion is a powerful means of improving performance and remains one of the most challenging methods to detect. Recent investigations have identified 3 candidate reticulocytes genes whose expression was significantly influenced by blood transfusion. Using quantitative reverse transcription polymer...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.2240

    authors: Salamin O,Mignot J,Kuuranne T,Saugy M,Leuenberger N

    更新日期:2018-03-01 00:00:00

  • UHPLC-MS/MS and UHPLC-HRMS identification of zolpidem and zopiclone main urinary metabolites and method development for their toxicological determination.

    abstract::Zolpidem and zopiclone (Z-compounds) are non-benzodiazepine hypnotics of new generation that can be used in drug-facilitated sexual assault (DFSA). Their determination in biological fluids, mainly urine, is of primary importance; nevertheless, although they are excreted almost entirely as metabolites, available method...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.1470

    authors: Strano Rossi S,Anzillotti L,Castrignanò E,Frison G,Zancanaro F,Chiarotti M

    更新日期:2014-03-01 00:00:00

  • Elimination profile of triamcinolone in urine following oral administration.

    abstract::Triamcinolone (T) is a glucocorticoid commonly used to relieve inflammation and treat arthritis, severe allergies, and asthma; however, it is banned by the World Anti-Doping Agency in competition for athletes when administered orally, intravenously, intramuscularly, or rectally. The minimum required performance limit ...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.2343

    authors: Chen TT,Tseng YC,Huang TY,Chang-Chien GP,Hsu MC

    更新日期:2018-05-01 00:00:00

  • Sensitive and selective spectrophotometric assay of gabapentin in capsules using sodium 1, 2-naphthoquinone-4-sulfonate.

    abstract::A simple, sensitive, and selective spectrophotometric method has been developed for the determination of gabapentin (GBP) in capsules. The method is based on the reaction of GBP and sodium 1,2-naphthoquinone-4-sulfonate (NQS) in the presence of Clark and Lubs buffer of pH 11 to form an orange-coloured product which wa...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.242

    authors: Abdulrahman SA,Basavaiah K

    更新日期:2011-10-01 00:00:00

  • Simultaneous quantification of digoxin, digitoxin, and their metabolites in serum using high performance liquid chromatography-tandem mass spectrometry.

    abstract::The study describes an LC-MS/MS method for simultaneous quantification of the cardiac glycosidic drugs digoxin and digitoxin and several of their metabolites. The assay represents a useful reference method for immunoassay-based tests, which are easily biased by the presence of metabolites of the target analytes or str...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.1781

    authors: Bylda C,Thiele R,Kobold U,Volmer DA

    更新日期:2015-10-01 00:00:00

  • Structural characterization of a degradation product of rocuronium using nanoelectrospray-high resolution mass spectrometry.

    abstract::Rocuronium bromide is a non-depolarizing neuromuscular blocking agent that causes rapid muscle relaxation after intravenous injection. Regulatory authorities for registration of pharmaceuticals for human use require the evaluation of the stability of active compounds under various stress conditions. Forced degradation...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.1751

    authors: Wegener O,Harms G,Volmer DA,Hayen H

    更新日期:2015-09-01 00:00:00

  • Pharmacokinetics and subjective effects of 1P-LSD in humans after oral and intravenous administration.

    abstract::1-Propanoyl-lysergic acid diethylamide (1P-LSD) appeared as a non-controlled alternative to LSD a few years ago. Although evidence is beginning to emerge from in vitro and animal studies that 1P-LSD might serve as a prodrug for LSD, an equivalent evaluation in humans is unavailable. Controlled oral and intravenous sel...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.2821

    authors: Grumann C,Henkel K,Brandt SD,Stratford A,Passie T,Auwärter V

    更新日期:2020-08-01 00:00:00

  • In vivo metabolism of the designer anabolic steroid hemapolin in the thoroughbred horse.

    abstract::Hemapolin (2α,3α-epithio-17α-methyl-5α-androstan-17β-ol) is a designer steroid that is an ingredient in several "dietary" and "nutritional" supplements available online. As an unusual chemical modification to the steroid A-ring could allow this compound to pass through antidoping screens undetected, the metabolism of ...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.2769

    authors: Waller CC,Weththasinghe SA,McClure L,Cawley AT,Suann C,Suann E,Sutherland E,Cooper E,Heather A,McLeod MD

    更新日期:2020-06-01 00:00:00

  • Considering gender in cannabinoid research: a step towards personalized treatment of marijuana addicts.

    abstract::Addiction is a complex disorder with interacting factors, including environmental factors, drug-induced neurobiological changes, comorbidity, personality traits and stress responsivity. Numerous genetic variants that affect these factors may work in concert to affect vulnerability and severity of addiction. Traditiona...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.1401

    authors: Fattore L

    更新日期:2013-01-01 00:00:00

  • Determination of partitioning of drug molecules using immobilized liposome chromatography and chemometrics methods.

    abstract::The quantitative structure-property relationship (QSPR) of drug molecules against the immobilized liposome chromatography partitioning (log K(s)) was studied. The genetic algorithm (GA) was employed to select the variables that resulted in the best-fitted models. After the variables were selected, the linear multivari...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.262

    authors: Noorizadeh H,Farmany A

    更新日期:2012-02-01 00:00:00