Abstract:
:Erythropoietin (EPO) has been misused in sports for many years due to its performance-enhancing effect. In the last decade, detection of abuse has been possible with isoelectric focusing (IEF) based on the different isoform profiles of endogenous and recombinant EPO. The release of new EPOs on the market, such as the recombinant erythropoietin epoetin delta (Dynepo™) and the chemically modified EPO, CERA (Mircera™) potentially represents analytical challenges to the fight against doping. This study set out to investigate the possibility of and the time window for detecting the administration of a single dose of Dynepo™ and CERA. Our results are in agreement with earlier findings that detection of Dynepo™ is best achieved by combining IEF with SDS-PAGE. Haematological parameters were monitored for possible effects due to the long half-life (130 hours) of CERA in blood. Interestingly, although several haematological parameters were significantly changed after the injection of CERA, the endogenous EPO signal was still present in all collected samples. Due to the long half-life and the large size of the CERA molecule (about 60 kDa), it was uncertain whether CERA would be excreted into urine in detectable amounts unless urine collection was preceded by strenuous physical exercise. We find that CERA can be detected in urine without prior exercise in several, but not all, subjects. CERA is nevertheless best detected in serum with regard to both probability and length of detection, in addition to stability in matrix over time.
journal_name
Drug Test Analjournal_title
Drug testing and analysisauthors
Dehnes Y,Hemmersbach Pdoi
10.1002/dta.270subject
Has Abstractpub_date
2011-05-01 00:00:00pages
291-9issue
5eissn
1942-7603issn
1942-7611journal_volume
3pub_type
杂志文章abstract::MicroRNAs (miRNAs) are small, non-protein coding transcripts involved in many cellular and physiological mechanisms. Recently, a new class of miRNA called 'circulating miRNAs' was found in cell-free body fluids such as plasma and urine. Circulating miRNAs have been shown to be very stable, specific, and sensitive biom...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.370
更新日期:2011-11-01 00:00:00
abstract::The objective of the present work was (1) to develop an in situ gelling ophthalmic delivery system by combining pluronic F127 and pluronic F68, with sparfloxacin; and (2) to examine the influence of incorporating a mucoadhesive polysaccharide such as sodium hyaluronate on the healing property due to bacterial keratiti...
journal_title:Drug testing and analysis
pub_type: 杂志文章
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abstract::Meconium drug testing is a non-invasive method to detect prenatal drug exposure, which can cause severe problems for the infant, indicating the need for follow-up measures to ensure the welfare of the child. Meconium samples for drug testing were collected from 143 infants as part of routine clinical work among addict...
journal_title:Drug testing and analysis
pub_type: 杂志文章,多中心研究
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abstract::The aim of this study was to investigate the effect of Morin on the pharmacokinetics of Piracetam in rats, in vitro enzyme kinetics and metabolic stability (high throughput) studies using human liver microsomes in UPLC. For pharmacokinetics studies, male Wistar rats were pretreated with Morin (10 mg/kg) for one week a...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.1382
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abstract::Selective androgen receptor modulators (SARMs) have great therapeutic potential in various diseases including cancer cachexia, sarcopenia, and osteoporosis, and the number of drug candidates has been growing over the last decade. The SARM drug candidates S-22 and S-23 belong to one of the most advanced groups of andro...
journal_title:Drug testing and analysis
pub_type: 杂志文章
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abstract::The present study demonstrates the applicability of a strategy involving use of liquid chromatography (LC) and liquid chromatography-mass spectrometry (LC-MS) techniques for the identification and characterization of minute quantities of degradation products, without their isolation from the reaction matrix in pure fo...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.116
更新日期:2010-02-01 00:00:00
abstract::The second programme of its kind globally, the highly successful, collaborative, productive and energizing IFDAT-International Forum for Drug and Alcohol Testing, was held in Barcelona, Spain, from 12-14 April. IFDAT was attended by over 100 delegates, conference sponsors and exhibitors from the international workplac...
journal_title:Drug testing and analysis
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journal_title:Drug testing and analysis
pub_type: 杂志文章
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更新日期:2012-09-01 00:00:00
abstract::Lysergic acid diethylamide (LSD) is a prototypical serotonergic psychedelic drug and the subject of many clinical investigations. In recent years, a range of lysergamides has emerged with the production of some being inspired by the existing scientific literature. Others, for example various 1-acyl substituted lyserga...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.2789
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journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.341
更新日期:2012-03-01 00:00:00
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journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.205
更新日期:2011-03-01 00:00:00
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journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.305
更新日期:2011-10-01 00:00:00
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journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.1608
更新日期:2014-10-01 00:00:00
abstract::Three sensitive, selective and precise stability-indicating methods for the determination of the anti-Alzheimer's drug, rivastigmine hydrogen tartrate (RIV) in the presence of its alkaline degradation product (major metabolite, NAP 226-90) and in pharmaceutical formulation were developed and validated. The first metho...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.121
更新日期:2010-05-01 00:00:00
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journal_title:Drug testing and analysis
pub_type: 杂志文章,评审
doi:10.1002/dta.128
更新日期:2010-04-01 00:00:00
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journal_title:Drug testing and analysis
pub_type: 杂志文章,meta分析,评审
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journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.2573
更新日期:2019-04-01 00:00:00
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journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.88
更新日期:2010-01-01 00:00:00
abstract::A series of regioisomeric bromodimethoxyamphetamines have mass spectra essentially equivalent to the controlled drug substance 4-Br-2,5-dimethoxyamphetamine (4-Br-2,5-DMA; DOB); all have molecular weight of 274 and major fragment ions in their electron ionization mass spectra at m/z 44 and m/z 230/232. The trifluoroac...
journal_title:Drug testing and analysis
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更新日期:2012-07-01 00:00:00
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journal_title:Drug testing and analysis
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journal_title:Drug testing and analysis
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journal_title:Drug testing and analysis
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journal_title:Drug testing and analysis
pub_type: 杂志文章
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更新日期:2018-05-01 00:00:00
abstract::A simple, sensitive, and selective spectrophotometric method has been developed for the determination of gabapentin (GBP) in capsules. The method is based on the reaction of GBP and sodium 1,2-naphthoquinone-4-sulfonate (NQS) in the presence of Clark and Lubs buffer of pH 11 to form an orange-coloured product which wa...
journal_title:Drug testing and analysis
pub_type: 杂志文章
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更新日期:2011-10-01 00:00:00
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journal_title:Drug testing and analysis
pub_type: 杂志文章
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更新日期:2015-10-01 00:00:00
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journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.1751
更新日期:2015-09-01 00:00:00
abstract::1-Propanoyl-lysergic acid diethylamide (1P-LSD) appeared as a non-controlled alternative to LSD a few years ago. Although evidence is beginning to emerge from in vitro and animal studies that 1P-LSD might serve as a prodrug for LSD, an equivalent evaluation in humans is unavailable. Controlled oral and intravenous sel...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.2821
更新日期:2020-08-01 00:00:00
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journal_title:Drug testing and analysis
pub_type: 杂志文章
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更新日期:2020-06-01 00:00:00
abstract::Addiction is a complex disorder with interacting factors, including environmental factors, drug-induced neurobiological changes, comorbidity, personality traits and stress responsivity. Numerous genetic variants that affect these factors may work in concert to affect vulnerability and severity of addiction. Traditiona...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.1401
更新日期:2013-01-01 00:00:00
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journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.262
更新日期:2012-02-01 00:00:00