Immunomagnetic beads-based isolation of erythropoietins from urine and blood for sports anti-doping control.

abstract：:Current doping trends overshadow the traditional interpretation of using prohibited performance-enhancing drugs to improve training and/or sports results. Its definition has evolved due to several recent cases of unconventional manipulation in sports. In this article, we present a case study of an intentional intoxica...

journal_title：Drug testing and analysis

authors： Baltazar-Martins G,Del Mar Plata M,Muñoz-Guerra J,Argaya J,Del Coso J

更新日期：2020-05-01 00:00:00

abstract：:A new, simple, rapid, accurate and precise high performance thin layer chromatography (HPTLC) method has been developed for the estimation of ondansetron hydrochloride in bulk and sublingual tablets. The mobile phase composition was chloroform : ethyl acetate : methanol : ammonia (9:5:4:0.1 v/v). Spectrodensitometric ...

journal_title：Drug testing and analysis

authors： Mujtaba A,Kohli K,Ali J,Baboota S

更新日期：2013-02-01 00:00:00

abstract：:The accuracy in predicting different chemometric methods was compared when applied on ordinary UV spectra and first order derivative spectra. Principal component regression (PCR) and partial least squares with one dependent variable (PLS1) and two dependent variables (PLS2) were applied on spectral data of pharmaceuti...

journal_title：Drug testing and analysis

authors： Riahi S,Hadiloo F,Milani SM,Davarkhah N,Ganjali MR,Norouzi P,Seyfi P

更新日期：2011-05-01 00:00:00

abstract：:The steroidal module of the Athlete Biological Passport (ABP) aims to detect doping with endogenous steroids, e.g. testosterone (T), by longitudinally monitoring several biomarkers. These biomarkers are ratios combined into urinary concentrations of testosterone and metabolically related steroids. However, it is evide...

journal_title：Drug testing and analysis

authors： Mullen J,Baekken LV,Törmäkangas T,Ekström L,Ericsson M,Hullstein IR,Schulze JJ

更新日期：2020-06-01 00:00:00

abstract：:Driving under the influence of drugs (DUID) is a worldwide problem. Several countries have adopted DUID legislations which prove their deterrent effect and impact on road safety. However, the use of new psychoactive substances (NPS) and prescription drugs is not known, as the applied roadside screening tests have not ...

journal_title：Drug testing and analysis

authors： Wille SMR,Richeval C,Nachon-Phanithavong M,Gaulier JM,Di Fazio V,Humbert L,Samyn N,Allorge D

更新日期：2018-03-01 00:00:00

abstract：:New Psychoactive Substances are now a feature of Australia's recreational drug market. Little is known, however, about the prevalence of use, the characteristics of people who use them and the relationship between the NPS and ecstasy markets. This study examined the prevalence and correlates of NPS use amongst a group...

journal_title：Drug testing and analysis

authors： Burns L,Roxburgh A,Matthews A,Bruno R,Lenton S,Van Buskirk J

更新日期：2014-07-01 00:00:00

abstract：:The synthesis of positional isomers of designer drugs is a common way of bypassing legal restrictions. For forensic case work, and especially for the legal assessment of cases, there is a need for screening methods capable of the unequivocal identification of positional isomers. The presented liquid chromatography-ele...

journal_title：Drug testing and analysis

authors： Grumann C,Auwärter V

更新日期：2018-02-18 00:00:00

abstract：:The determination of protein- or peptide-based performance-enhancing pharmaceuticals has gained increasing attention in sports drug testing in recent decades. Proteases possess a high potential for use in the manipulation of regular urine specimens, interfering with established doping control procedures for proteins o...

journal_title：Drug testing and analysis

authors： Thomas A,Kohler M,Walpurgis K,Schänzer W,Thevis M

更新日期：2009-02-01 00:00:00

abstract：:Selective androgen receptor modulators (SARMs) have great therapeutic potential in various diseases including cancer cachexia, sarcopenia, and osteoporosis, and the number of drug candidates has been growing over the last decade. The SARM drug candidates S-22 and S-23 belong to one of the most advanced groups of andro...

journal_title：Drug testing and analysis

authors： Thevis M,Gerace E,Thomas A,Beuck S,Geyer H,Schlörer N,Kearbey JD,Dalton JT,Schänzer W

更新日期：2010-11-01 00:00:00

abstract：:Eurycoma longifolia Jack (Tongkat Ali, Simaroubaceae) is a medicinal plant endemic to South-East Asia. For centuries, different parts of the plant have been used as a natural remedy to treat fever, hypertension, or sexual insufficiency. Today, Eurycoma longifolia preparations are commercially available and advertised ...

journal_title：Drug testing and analysis

authors： Bräuer P,Anielski P,Schwaiger S,Stuppner H,Tran TVA,Vollmer G,Zierau O,Thieme D,Keiler AM

更新日期：2019-01-01 00:00:00

abstract：:Knowing that polyunsaturated fatty acids can lead to the formation of potentially toxic aldehydes as secondary oxidation products, an analytical method using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) detection was developed to measure the concentration of eight aldehydes in animal feed: malo...

journal_title：Drug testing and analysis

authors： Douny C,Bayram P,Brose F,Degand G,Scippo ML

更新日期：2016-05-01 00:00:00

abstract：:Cobalt is a well-established inducer of hypoxia-like responses, which can cause gene modulation at the hypoxia inducible factor pathway to induce erythropoietin transcription. Cobalt salts are orally active, inexpensive, and easily accessible. It is an attractive blood doping agent for enhancing aerobic performance. I...

journal_title：Drug testing and analysis

authors： Ho EN,Chan GH,Wan TS,Curl P,Riggs CM,Hurley MJ,Sykes D

更新日期：2015-01-01 00:00:00

abstract：:In the context of post-marketing surveillance supporting public-health authorities to take evidence-based decisions to fight the spread of poor-quality medicines, the quality of antimalarial artemether-lumefantrine (AL) medicines was assessed in the Democratic Republic of the Congo (DRC). A total of 150 samples of AL-...

journal_title：Drug testing and analysis

authors： Mufusama JP,Ndjoko Ioset K,Feineis D,Hoellein L,Holzgrabe U,Bringmann G

更新日期：2018-10-01 00:00:00

abstract：:We recently published two protocols for the detection of Sotatercept (ACE-011, ACVR2A-Fc) and Luspatercept (ACE-536, ACVR2B-Fc) in human serum. Both methods used covalently immobilized antibodies on agarose beads for immunoprecipitation and SAR-PAGE/Western blotting for detection. Disadvantages were the relatively hig...

journal_title：Drug testing and analysis

authors： Reichel C,Gmeiner G,Walpurgis K,Thevis M

更新日期：2018-11-01 00:00:00

abstract：:The objective of the present work was (1) to develop an in situ gelling ophthalmic delivery system by combining pluronic F127 and pluronic F68, with sparfloxacin; and (2) to examine the influence of incorporating a mucoadhesive polysaccharide such as sodium hyaluronate on the healing property due to bacterial keratiti...

journal_title：Drug testing and analysis

authors： Nesseem DI

更新日期：2011-02-01 00:00:00

abstract：:An orthogonal signal correction-partial least squares (OSC-PLS) method was developed for the simultaneous spectrophotometric determination of orotic acid (OA), creatinine (CRE), and uric acid (UA) in spiked real samples. By multivariate calibration methods, such as PLS regression, it is possible to obtain a model adju...

journal_title：Drug testing and analysis

authors： Khajehsharifi H,Tavallali H,Shekoohi M,Sadeghi M

更新日期：2013-05-01 00:00:00

abstract：:A simple, sensitive, and selective spectrophotometric method has been developed for the determination of gabapentin (GBP) in capsules. The method is based on the reaction of GBP and sodium 1,2-naphthoquinone-4-sulfonate (NQS) in the presence of Clark and Lubs buffer of pH 11 to form an orange-coloured product which wa...

journal_title：Drug testing and analysis

authors： Abdulrahman SA,Basavaiah K

更新日期：2011-10-01 00:00:00

abstract：:Dihydroartemisinin (DHA) and piperaquine (PPQ) are two drugs used in an artemisinin-based combination therapy (ACT). The circulation of counterfeit antimalarial drugs demands the development of simple, point-of-care (POC) tests for monitoring drug quality. Here we aimed to design an antibody-based lateral flow dipstic...

journal_title：Drug testing and analysis

authors： Ning X,Tan G,Chen X,Wang M,Wang B,Cui L

更新日期：2019-09-01 00:00:00

abstract：:A simple and sensitive method is described for voltammetric determination of promethazine hydrochloride (PMZ), a widely used phenothiazine drug, based on its electrochemical oxidation at a multi-wall carbon nanotube (MWCNT) modified glassy carbon electrode (GCE). Compared with bare GCE, the MWCNT-modified GCE exhibite...

journal_title：Drug testing and analysis

authors： Xi X,Ming L,Liu J

更新日期：2011-03-01 00:00:00

abstract：:Since the appearance of recombinant human growth hormone (rhGH) in the 1980s, its expansion and acquisition through the black market has increased, so the detection of its abuse continues to be a challenge. New biomarkers that are more reliable and sensitive, allowing a larger detection window, are still needed. In th...

journal_title：Drug testing and analysis

authors： Ferro P,Ventura R,Pérez-Mañá C,Farré M,Segura J

更新日期：2017-07-01 00:00:00

abstract：:Xenon is a modern inhalative anaesthetic with a very low solubility in tissues providing rapid elimination and weaning from anaesthesia. Besides its anaesthetic properties, Xenon promotes the endogenous erythropoietin biosynthesis and thus has been enlisted as prohibited substance by the World Anti-Doping Agency (WADA...

journal_title：Drug testing and analysis

authors： Schaefer MS,Piper T,Geyer H,Schneemann J,Neukirchen M,Thevis M,Kienbaum P

更新日期：2017-05-01 00:00:00

abstract：:Recombinant human erythropoietin (rHuEPO) is used as doping a substance. Anti-doping efforts include urine and blood testing and monitoring the athlete biological passport (ABP). As data on the performance of these methods are incomplete, this study aimed to evaluate the performance of two common urine assays and the ...

journal_title：Drug testing and analysis

authors： Heuberger JAAC,van Eenoo P,Rotmans JI,Gal P,Stuurman FE,Post TE,Daniels JMA,Ram H,de Hon O,Burggraaf J,Cohen AF

更新日期：2019-09-01 00:00:00

abstract：:Addiction is a complex disorder with interacting factors, including environmental factors, drug-induced neurobiological changes, comorbidity, personality traits and stress responsivity. Numerous genetic variants that affect these factors may work in concert to affect vulnerability and severity of addiction. Traditiona...

journal_title：Drug testing and analysis

authors： Fattore L

更新日期：2013-01-01 00:00:00

abstract：:Tretoquinol (trimetoquinol), a β2-agonist, has been explicitly listed on the World Anti-Doping Agency Prohibited List 2019 since January 2019; however, it has been distributed as an antiasthmatic on the medical market. This study aimed to develop a liquid chromatography-tandem mass spectrometric method for the quantif...

journal_title：Drug testing and analysis

authors： Okano M,Miyamoto A,Sato M,Kageyama S

更新日期：2019-11-01 00:00:00

abstract：:At present, there is a growing trend toward the intentional adulteration of dietary supplements (DS) with synthetic pharmaceuticals, which represents an alarming emerging risk to consumers and a serious problem for regulatory agencies. An amazing array of synthetic drugs and their analogues have been reported as adult...

journal_title：Drug testing and analysis

authors： Muschietti L,Redko F,Ulloa J

更新日期：2020-07-01 00:00:00

abstract：:Phosphatidylethanol 16:0/18:1 (PEth) is the most abundant homologue of the phosphatidylethanol group of phospholipids. Formed only in the presence of ethanol, PEth is used as a biomarker in whole blood to provide information about the consumption of alcohol. As information on the storage life of PEth is essential for ...

journal_title：Drug testing and analysis

authors： Dumitrascu C,van de Lavoir MCA,Neels H,Covaci A,Jacobs W,D'Hondt D,van Nuijs ALN

更新日期：2021-01-04 00:00:00

abstract：:Amphetamine (AMP) is used as an illicit drug and also for the treatment of attention deficit hyperactivity disorder (ADHD). Respective drugs most often contain the enantiomer (S)-AMP as active compound or (S)-AMP is formed from the prodrug lisdexamfetamine (Elvanse®) whereas the illicit drug is usually traded as racem...

journal_title：Drug testing and analysis

authors： Binz TM,Williner E,Strajhar P,Dolder PC,Liechti ME,Baumgartner MR,Kraemer T,Steuer AE

更新日期：2018-02-01 00:00:00

abstract：:The availability of a real-time assay to experimentally investigate the release of encapsulated proteins would be beneficial given the interest in the use of liposomes as a drug delivery vehicle. Although simple assays for small molecular weight substances exist, assays to evaluate macromolecules do not. Here we descr...

journal_title：Drug testing and analysis

authors： Mujoo H,Reynolds JNJ,Tucker IG

更新日期：2018-06-01 00:00:00

abstract：:A sensitive and selective ultra fast liquid chromatography (UFLC) method has been developed and validated for the determination of a potent and novel antitubercular compound S006-830 in Sprague Dawley (SD) rat plasma. Samples were extracted and processed by protein precipitation method using acetonitrile. Chromatograp...

journal_title：Drug testing and analysis

authors： Singh Y,Hidau MK,Misra A,Kushwaha HN,Tiwari A,Sharma AK,Singh SK

更新日期：2015-08-01 00:00:00

abstract：:Rocuronium bromide is a non-depolarizing neuromuscular blocking agent that causes rapid muscle relaxation after intravenous injection. Regulatory authorities for registration of pharmaceuticals for human use require the evaluation of the stability of active compounds under various stress conditions. Forced degradation...

journal_title：Drug testing and analysis

authors： Wegener O,Harms G,Volmer DA,Hayen H

更新日期：2015-09-01 00:00:00