Abstract:
:An orthogonal signal correction-partial least squares (OSC-PLS) method was developed for the simultaneous spectrophotometric determination of orotic acid (OA), creatinine (CRE), and uric acid (UA) in spiked real samples. By multivariate calibration methods, such as PLS regression, it is possible to obtain a model adjusted to the concentration values of the mixtures used in the calibration range. The effect of OSC used to remove the information unrelated to the target variables is studied. In this study, the calibration model is based on absorption spectra in the 220-320 nm rang for 36 different mixtures of OA, CRE and UA. Calibration matrices contained 1.74-47.00 of OA, 1.13-33.95 of CRE, and 1.68-28.58 of UA in µg/ml. The number of principal component for OA, CRE, and UA with OSC were 3, 4, and 4, and 4, 6, and 5, without OSC, respectively. The evaluation of the prediction errors for the prediction set reveals that the OSC-treated data give substantially lower root mean square error of prediction (RMSEP) values than the original data. The RMSEP for OA, CRE, and UA with OSC were 0.69, 0.20, and 0.53 and 0.80, 0.69, and 0.73 without OSC, respectively. The proposed method was applied for the simultaneous determination of OA, CRE, and UA in spiked biological fluids with satisfactory results.
journal_name
Drug Test Analjournal_title
Drug testing and analysisauthors
Khajehsharifi H,Tavallali H,Shekoohi M,Sadeghi Mdoi
10.1002/dta.375subject
Has Abstractpub_date
2013-05-01 00:00:00pages
353-60issue
5eissn
1942-7603issn
1942-7611journal_volume
5pub_type
杂志文章abstract::Δ(9) -Tetrahydrocannabinol (THC) is the most commonly analyzed cannabinoid in oral fluid (OF); however, its metabolite 11-nor-9-carboxy-THC (THCCOOH) offers the advantage of documenting active consumption, as it is not detected in cannabis smoke. Analytical challenges such as low (ng/L) THCCOOH OF concentrations hampe...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.1753
更新日期:2015-08-01 00:00:00
abstract::The evolving nature of new psychoactive substances (NPS) - often referred to as 'legal highs', 'designer drugs' or 'bath salts' - presents an evolving challenge for toxicologists. Apart from the detection and identification of these compounds, further analytical challenges may arise from the presence of possible metab...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.1576
更新日期:2014-07-01 00:00:00
abstract::Testosterone and related compounds are the most recurrent doping substances. The steroid profile, consisting of the quantification of testosterone and its metabolites, has been described as the most significant biomarker to detect doping with pseudo-endogenous anabolic steroids. The steroidal module of the Athlete Bio...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.1851
更新日期:2016-07-01 00:00:00
abstract::Selective androgen receptor modulators (SARMs) are a group of anabolic enhancer drugs posing threats to the integrity of animal sports and the safety of animal-derived foods. The current research describes for the first time the development of a semi-quantitative assay for the monitoring of SARM family compounds in bl...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.2875
更新日期:2020-09-01 00:00:00
abstract::Three legal highs; nitracaine (3-(diethylamino)-2,2-dimethylpropyl 4-nitrobenzoate), methoxypiperamide (MEOP, (4-methoxyphenyl)(4-methylpiperazin-1-yl)methanone) and mephtetramine (MTTA, 2-((methylamino)methyl)-3,4-dihydronaphthalen-1(2H)-one) appeared in 2013 as new psychoactive substances (NPS) on Internet websites ...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.1616
更新日期:2014-07-01 00:00:00
abstract::The amphetamine molecule contains a chiral center and its enantiomers exhibit differences in pharmacological effects, with the S-enantiomer mediating most of the central nervous system stimulating activity. The majority of prescribed amphetamine consists of the pure S-enantiomer, but therapeutic formulations containin...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.2879
更新日期:2020-09-01 00:00:00
abstract::1-Propanoyl-lysergic acid diethylamide (1P-LSD) appeared as a non-controlled alternative to LSD a few years ago. Although evidence is beginning to emerge from in vitro and animal studies that 1P-LSD might serve as a prodrug for LSD, an equivalent evaluation in humans is unavailable. Controlled oral and intravenous sel...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.2821
更新日期:2020-08-01 00:00:00
abstract::The tiletamine-zolazepam (TZ) combination is an anaesthetic drug commonly used in veterinary medicine. It is an equal amount combination of tiletamine, a dissociative anaesthetic pharmacologically classified as an N-methyl-D-aspartate (NMDA) receptor antagonist, and zolazepam, a benzodiazepine tranquilizer. There are ...
journal_title:Drug testing and analysis
pub_type: 杂志文章,评审
doi:10.1002/dta.1987
更新日期:2016-08-01 00:00:00
abstract::The presence of erythropoiesis stimulating agents (ESAs) in the urine samples collected from athletes is detected using traditional Western blotting following either size-based separation (SDS/SAR-PAGE) or isoelectric focusing (IEF). Although there is an important testing effort, there is little doubt that ESAs are st...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.2528
更新日期:2018-11-01 00:00:00
abstract::Higenamine was included in the World Anti-Doping Agency (WADA) Prohibited Substances and Methods List as a β2 -adrenoceptor agonist in 2017, thereby resulting in its prohibition both in and out of competition. The present mini review describes the physiology and pharmacology of adrenoceptors, summarizes the literature...
journal_title:Drug testing and analysis
pub_type: 杂志文章,评审
doi:10.1002/dta.2992
更新日期:2020-12-26 00:00:00
abstract::Three multivariate calibration methods, including classical least square with nonzero intercept (CLS), principal component regression (PCR) and partial least square (PLS), have been used for the determination of pyritinol dihydrochloride in the presence of its degradation product. The CLS, PCR and PLS techniques are u...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.37
更新日期:2009-05-01 00:00:00
abstract::The direct alcohol marker ethyl glucuronide (EtG) is widely used for the assessment of alcohol consumption behavior and abstinence monitoring by hair analysis. We investigated the influence of chlorinated swimming pool water on EtG concentrations in hair in comparison to deionized water (Milli-Q) containing no chlorin...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.2295
更新日期:2018-04-01 00:00:00
abstract::Flubromazolam is a triazole benzodiazepine with high potency and long-lasting central nervous system depressant effects; however, limited data about its pharmacokinetics are available. Here, we report in vitro studies of the human flubromazolam metabolism analyzed by liquid chromatography high-resolution mass spectrom...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.2146
更新日期:2017-08-01 00:00:00
abstract::According to the World Anti-Doping Agency (WADA) technical document for erythropoiesis stimulating agents (ESA) analysis (TD2014EPO), double-blotting of serum/plasma samples is mandatory for all analysis by isoelectric focusing (IEF) and for the confirmation procedures (CP) performed by SDS-PAGE or SAR-PAGE. The goal ...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.2320
更新日期:2017-11-01 00:00:00
abstract::Phosphatidylethanol 16:0/18:1 (PEth) is the most abundant homologue of the phosphatidylethanol group of phospholipids. Formed only in the presence of ethanol, PEth is used as a biomarker in whole blood to provide information about the consumption of alcohol. As information on the storage life of PEth is essential for ...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.2995
更新日期:2021-01-04 00:00:00
abstract::New bioanalytical assays were developed, validated, and applied in a clinical study for quantitative measurement of acetaminophen concentrations in blood and plasma samples. Furthermore, after validation, the bioanalytical assays were used for determination of pharmacokinetics within a group of six healthy male human ...
journal_title:Drug testing and analysis
pub_type: 临床试验,杂志文章
doi:10.1002/dta.1582
更新日期:2014-06-01 00:00:00
abstract::Drug and alcohol abuse is a concern for many European companies, especially those having safety-critical jobs. It is not uncommon for European companies to establish a drug policy with little or no provision for drug testing. The European Union (EU) has launched a number of initiatives in its fight against drugs. Ther...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.147
更新日期:2010-08-01 00:00:00
abstract::According to French law, the roadside testing for drugs of abuse (DOA) should be performed in oral fluid (OF) using an immunological screening kit. If the screening is positive, confirmation has to be done in OF collected by a special swab, called the FLOQSwab™ (FS). Unlike other sampling kits, this device was not des...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.2563
更新日期:2019-06-01 00:00:00
abstract::At present, there is a growing trend toward the intentional adulteration of dietary supplements (DS) with synthetic pharmaceuticals, which represents an alarming emerging risk to consumers and a serious problem for regulatory agencies. An amazing array of synthetic drugs and their analogues have been reported as adult...
journal_title:Drug testing and analysis
pub_type: 杂志文章,评审
doi:10.1002/dta.2806
更新日期:2020-07-01 00:00:00
abstract::Therapeutic drug monitoring (TDM) of immunosuppressive drugs (ISDs) with a narrow therapeutic index is an increasingly popular tool for minimizing drug toxicity while maximizing the prevention of graft loss and organ rejection. This review focuses on trends regarding analytical methods for the TDM of ISDs since 2011. ...
journal_title:Drug testing and analysis
pub_type: 杂志文章,评审
doi:10.1002/dta.2290
更新日期:2018-01-01 00:00:00
abstract::Driving under the influence of drugs (DUID) is a worldwide problem. Several countries have adopted DUID legislations which prove their deterrent effect and impact on road safety. However, the use of new psychoactive substances (NPS) and prescription drugs is not known, as the applied roadside screening tests have not ...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.2232
更新日期:2018-03-01 00:00:00
abstract::In 2005 and 2008, studies reported that cannabis in England had become dominated by the sinsemilla (unseeded female) form. The average potency (Δ9 -tetrahydrocannabinol [THC] content) of this material had doubled over the previous decade. Cannabis resin then circulating contained approximately equal ratios of THC and ...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.2368
更新日期:2018-04-01 00:00:00
abstract::The impact of dehydroepiandrosterone (DHEA) administration has been widely studied for anti-doping purposes in men, whereas only a few studies have been performed in women. In the present study, the impact of DHEA on the steroid profile parameters and their carbon isotopic ratios was explored. Eleven healthy young wom...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.2519
更新日期:2018-11-01 00:00:00
abstract::Synthetic cannabinoids (SCs), mimicking the psychoactive effects of cannabis, consist of a vast array of structurally diverse compounds. A novel compound belonging to the SC family, (1-(cyclohexylmethyl)-1H-indol-3-yl)-(2,2,3,3-tetramethylcyclopropyl)methanone (named TMCP-CHM in this article) contains a cyclopropane r...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.2668
更新日期:2019-09-01 00:00:00
abstract::In Australia, drug monitoring systems have been in place for more than a decade allowing for the measurement of ongoing trends in drug use and the detection of new drugs. The Drug Trends Unit at the National Drug and Alcohol Research Centre monitors drugs through four separate systems. The Illicit Drug Reporting Syste...
journal_title:Drug testing and analysis
pub_type: 杂志文章,评审
doi:10.1002/dta.1613
更新日期:2014-07-01 00:00:00
abstract::New Psychoactive Substances are now a feature of Australia's recreational drug market. Little is known, however, about the prevalence of use, the characteristics of people who use them and the relationship between the NPS and ecstasy markets. This study examined the prevalence and correlates of NPS use amongst a group...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.1626
更新日期:2014-07-01 00:00:00
abstract::Gamma-hydroxybutyrate (GHB) has been used as a recreational drug since the 1990s and over the last few years there has been increasing use of its analogues gamma-butyrolactone (GBL) and to a lesser extent 1,4-butanediol (1,4BD). This review will summarize the literature on the pharmacology of these compounds; the patt...
journal_title:Drug testing and analysis
pub_type: 杂志文章,评审
doi:10.1002/dta.292
更新日期:2011-07-01 00:00:00
abstract::Several clinical-grade immunoassays exist for the specific measurement of hGH or its isoforms in blood but there is an urgent need to apply these same reliable assays to the measurement of hGH in urine as a preferred 'non-invasive' biofluid. Unfortunately, conventional hGH immunoassays cannot attain the sensitivity re...
journal_title:Drug testing and analysis
pub_type: 杂志文章,评审
doi:10.1002/dta.96
更新日期:2009-09-01 00:00:00
abstract::The synthetic tryptamine 5-methoxy-N-methyl-N-isopropyltryptamine (5-MeO-MiPT) has recently been abused as a hallucinogenic drug in Germany and Switzerland. This study presents a case of 5-MeO-MiPT intoxication and the structural elucidation of metabolites in pooled human liver microsomes (pHLM), blood, and urine. Mic...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.2245
更新日期:2018-03-01 00:00:00
abstract::Prenylamine is a vasodilator of phenylalkylamine structure and was used for the treatment of angina pectoris, until reports of undesirable effects including ventricular tachycardia led to a decreasing use of the drug in the 1980s. Metabolic N-dealkylation of orally ingested prenylamine can liberate amphetamine in huma...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.1388
更新日期:2012-09-01 00:00:00