Abstract:
:New bioanalytical assays were developed, validated, and applied in a clinical study for quantitative measurement of acetaminophen concentrations in blood and plasma samples. Furthermore, after validation, the bioanalytical assays were used for determination of pharmacokinetics within a group of six healthy male human volunteers after admission of a single oral dose of 500 mg of acetaminophen. Quantitative analyses were done by means of liquid chromatography-high resolution mass spectrometry and blood samples were collected at various sampling time points using different peripheral blood microsampling techniques. Post-dose peripheral collected blood samples were applied for the preparation of dry blood spots, dried matrix on paper discs, and peripheral plasma. Pharmacokinetic parameters determined were clearance (Cl), area under the curve (AUC), volume of distribution (Vd ), peak concentration (Cmax ), time of occurrence of peak concentration (Tmax ) and half-life time (T½ ). Observed pharmacokinetic values were not statistically (ANOVA) different compared to in literature reported values based on venous blood collection. The present pilot study demonstrated the feasibility of peripheral blood microsampling techniques in combination with quantitative liquid chromatography-high resolution mass spectrometry analysis for the determination of pharmacokinetics in clinical studies.
journal_name
Drug Test Analjournal_title
Drug testing and analysisauthors
Rincón JP,Meesters RJdoi
10.1002/dta.1582subject
Has Abstractpub_date
2014-06-01 00:00:00pages
568-77issue
6eissn
1942-7603issn
1942-7611journal_volume
6pub_type
临床试验,杂志文章abstract::In Italy, Workplace Drug Testing (WDT) has been compulsory by law for specific categories of workers since 2008, offering the opportunity to compare studies conducted within a single regulatory framework. The aims of this paper are to estimate the overall prevalence of WDT positivity (at screening survey) among Italia...
journal_title:Drug testing and analysis
pub_type: 杂志文章,meta分析,评审
doi:10.1002/dta.2189
更新日期:2017-06-01 00:00:00
abstract::A novel poly (vinyl chloride) PVC membrane sensor for Fe(2+) ions is described. The sensor is based on the use of newly synthesized chiral 2,6-bis-(carboxamide methyl ester)pyridine derivative as neutral ionophore in plasticized PVC membrane. The sensor display a fast, stable and near-Nernstian response over a relativ...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.231
更新日期:2011-06-01 00:00:00
abstract::Musk, the dried secretion of the musk pod (sac) of adult male musk deer, has been used as traditional Chinese Medicine (TCM) in China and south-east Asian countries for thousands of years. Due to the anabolic steroid component in this TCM, musk preparations have been included in the list of medical products containing...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.2293
更新日期:2017-11-01 00:00:00
abstract::Deschloroketamine (2-(methylamino)-2-phenyl-cyclohexanone) is a ketamine analog belonging to a group of dissociative anesthetics, which have been distributed within the illicit market since 2015. However, it was also being sold as 'ketamine' misleading people to believe that they were getting genuine ketamine. Dissoci...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.2726
更新日期:2020-03-01 00:00:00
abstract::N-Methyl-3-phenyl-norbornan-2-amine (N-methyl-3-phenylbicyclo[2.2.1]heptan-2-amine, Camfetamine(™) ) is available from a number of online legal highs/research chemicals' vendors. Although it was developed as an analeptic by Merck in the early 1960s, it was never commercialized. However, the Association of Independent ...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.411
更新日期:2013-04-01 00:00:00
abstract::The use of corticosteroids, such as betamethasone, in performance horses is tightly regulated. The objective of the current study was to describe the plasma pharmacokinetics of betamethasone as well as time-related urine and synovial fluid concentrations following intra-articular administration to horses. Twelve racin...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.2170
更新日期:2017-09-01 00:00:00
abstract::A series of regioisomeric bromodimethoxyamphetamines have mass spectra essentially equivalent to the controlled drug substance 4-Br-2,5-dimethoxyamphetamine (4-Br-2,5-DMA; DOB); all have molecular weight of 274 and major fragment ions in their electron ionization mass spectra at m/z 44 and m/z 230/232. The trifluoroac...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.409
更新日期:2012-07-01 00:00:00
abstract::The steroidal module of the Athlete Biological Passport (ABP) aims to detect doping with endogenous steroids, e.g. testosterone (T), by longitudinally monitoring several biomarkers. These biomarkers are ratios combined into urinary concentrations of testosterone and metabolically related steroids. However, it is evide...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.2778
更新日期:2020-06-01 00:00:00
abstract::Harm reduction services, including needle-exchange programmes, have been implemented in Switzerland for over 20 years. Their main aim is to lessen the negative social and/or physical consequences associated with illicit drug consumption and, therefore, improve prevention messages. To this end, knowledge of illicit dru...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.2335
更新日期:2018-05-01 00:00:00
abstract::Two primary animal models persist for assessing hallucinogenic potential of novel compounds and for examining the pharmacological and neurobiological substrates underlying the actions of classical hallucinogens, the two-lever drug discrimination procedure and the drug-induced head-twitch response (HTR) in rodents. The...
journal_title:Drug testing and analysis
pub_type: 杂志文章,评审
doi:10.1002/dta.1333
更新日期:2012-07-01 00:00:00
abstract::Fast and unequivocal drug detection is of considerable importance in numerous fields of analytical chemistry, and today mass spectrometry-based approaches are often the method of choice due to their sensitive and specific nature. Mass spectrometry is in constant flux with innovations and thus supports the development ...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.344
更新日期:2012-03-01 00:00:00
abstract::The purpose of this research is to validate the biomarker-based approach for the detection of doping with recombinant human growth hormone (rhGH) in sport. The GH-2000 project proposed an indirect method for the detection of exogenously administered growth hormone (GH) based on the measurement of the GH-dependent mark...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.1423
更新日期:2012-10-01 00:00:00
abstract::Flubromazolam is a triazole benzodiazepine with high potency and long-lasting central nervous system depressant effects; however, limited data about its pharmacokinetics are available. Here, we report in vitro studies of the human flubromazolam metabolism analyzed by liquid chromatography high-resolution mass spectrom...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.2146
更新日期:2017-08-01 00:00:00
abstract::A simple, sensitive, and selective spectrophotometric method has been developed for the determination of gabapentin (GBP) in capsules. The method is based on the reaction of GBP and sodium 1,2-naphthoquinone-4-sulfonate (NQS) in the presence of Clark and Lubs buffer of pH 11 to form an orange-coloured product which wa...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.242
更新日期:2011-10-01 00:00:00
abstract::Gamma-hydroxybutyrate (GHB) has been used as a recreational drug since the 1990s and over the last few years there has been increasing use of its analogues gamma-butyrolactone (GBL) and to a lesser extent 1,4-butanediol (1,4BD). This review will summarize the literature on the pharmacology of these compounds; the patt...
journal_title:Drug testing and analysis
pub_type: 杂志文章,评审
doi:10.1002/dta.292
更新日期:2011-07-01 00:00:00
abstract::A simple and sensitive method is described for voltammetric determination of promethazine hydrochloride (PMZ), a widely used phenothiazine drug, based on its electrochemical oxidation at a multi-wall carbon nanotube (MWCNT) modified glassy carbon electrode (GCE). Compared with bare GCE, the MWCNT-modified GCE exhibite...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.205
更新日期:2011-03-01 00:00:00
abstract::MSforID represents a database of tandem mass spectral data obtained from (quasi-)molecular ions produced by atmospheric pressure ionization methods. At the current stage of development the library contains 12 122 spectra of 1208 small (bio-)organic molecules. The present work was aimed to evaluate the performance of t...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.341
更新日期:2012-03-01 00:00:00
abstract::Even though hair analysis often seems to be the best choice for retrospective monitoring of cocaine intake, differentiating between incorporated cocaine and external contamination is widely debated. In this study we report results obtained in 90 hair samples from addicts. All samples were analyzed for cocaine, benzoyl...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.1903
更新日期:2017-02-01 00:00:00
abstract::The method of high sensitive gas chromatographic/time-of-flight mass-spectrometric (GC/TOF-MS) analysis of steroids was developed. Low-resolution TOF-MS instrument (with fast spectral acquisition rate) was used. This method is based on the formation of the silyl derivatives of steroids; exchange of the reagent mixture...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.221
更新日期:2011-04-01 00:00:00
abstract::Autologous blood transfusion is a powerful means of improving performance and remains one of the most challenging methods to detect. Recent investigations have identified 3 candidate reticulocytes genes whose expression was significantly influenced by blood transfusion. Using quantitative reverse transcription polymer...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.2240
更新日期:2018-03-01 00:00:00
abstract::Dihydroartemisinin (DHA) and piperaquine (PPQ) are two drugs used in an artemisinin-based combination therapy (ACT). The circulation of counterfeit antimalarial drugs demands the development of simple, point-of-care (POC) tests for monitoring drug quality. Here we aimed to design an antibody-based lateral flow dipstic...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.2656
更新日期:2019-09-01 00:00:00
abstract::The detection of an intact ester of testosterone in plasma is leading towards unequivocal proof of the administration of exogenous testosterone. In the current study, a sensitive screening method for the detection of nine testosterone esters in human plasma was developed. By preparing oxime derivatives of intact testo...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.1560
更新日期:2013-11-01 00:00:00
abstract::Classic examples of psychoactive arylcycloalkylamines include ketamine and 1-(1-phenylcyclohexyl)piperidine (PCP) and many others serve as important structural templates for neuropharmacological research. The recent emergence of PCP analogues that can be obtained from internet retailers requires the implementation of ...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.1468
更新日期:2014-07-01 00:00:00
abstract::Inhibition of acetylcholinesterase (AChE) is the main mechanism of action of organophosphorus compounds (OP), and AChE reactivators (oximes) are at present the only causal therapeutic approach. Being the key target of OP toxicity, AChE may serve as a valuable tool for diagnosis of OP exposure as well as for the invest...
journal_title:Drug testing and analysis
pub_type: 杂志文章,评审
doi:10.1002/dta.337
更新日期:2012-03-01 00:00:00
abstract::Detection of the abuse of synthetic steroids in food production is nowadays relatively straightforward using modern techniques such as gas or liquid chromatography coupled to mass spectrometry (GC-MS/MS or LC-MS/MS, respectively). However, proving the abuse of 'endogenous' (or naturally occurring) steroids is more dif...
journal_title:Drug testing and analysis
pub_type: 杂志文章,评审
doi:10.1002/dta.1354
更新日期:2012-08-01 00:00:00
abstract::Designer drugs belong to a group of legally or illegally produced substances that are structurally and pharmacologically very similar to illicit drugs. In the past, designer drugs were often used during all-night dance parties, but they are now consumed in multiple settings from college bars to parks to private house ...
journal_title:Drug testing and analysis
pub_type: 杂志文章,评审
doi:10.1002/dta.210
更新日期:2011-09-01 00:00:00
abstract::Despite the implementation of a new blanket scheduling system in 2013, new psychoactive substance (NPS) abuse remains a serious social concern in Japan. We present a fatal intoxication case involving 5F-ADB (methyl 2-[1-(5-fluoropentyl)-1H-indazole-3-carboxamido]-3,3-dimethylbutanoate) and diphenidine. Postmortem bloo...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.2215
更新日期:2018-02-01 00:00:00
abstract::Doping with natural steroids can be detected by evaluating the urinary concentrations and ratios of several endogenous steroids. Since these biomarkers of steroid doping are known to present large inter-individual variations, monitoring of individual steroid profiles over time allows switching from population-based to...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.206
更新日期:2010-11-01 00:00:00
abstract::Zebrafish (Danio rerio) water tank (ZWT) approach was investigated as an alternative model for metabolism studies based on six different experiments with four model compounds. Sibutramine was applied for the multivariate optimization of ZWT conditions, also for the comparison of the metabolism among ZWT, humans and mi...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.2523
更新日期:2018-11-01 00:00:00
abstract::To date, substances such as Mildronate (Meldonium) are not on the radar of anti-doping laboratories as the compound is not explicitly classified as prohibited. However, the anti-ischemic drug Mildronate demonstrates an increase in endurance performance of athletes, improved rehabilitation after exercise, protection ag...
journal_title:Drug testing and analysis
pub_type: 杂志文章
doi:10.1002/dta.1788
更新日期:2015-11-01 00:00:00