Evaluation of peripheral blood microsampling techniques in combination with liquid chromatography-high resolution mass spectrometry for the determination of drug pharmacokinetics in clinical studies.

Abstract:

:New bioanalytical assays were developed, validated, and applied in a clinical study for quantitative measurement of acetaminophen concentrations in blood and plasma samples. Furthermore, after validation, the bioanalytical assays were used for determination of pharmacokinetics within a group of six healthy male human volunteers after admission of a single oral dose of 500 mg of acetaminophen. Quantitative analyses were done by means of liquid chromatography-high resolution mass spectrometry and blood samples were collected at various sampling time points using different peripheral blood microsampling techniques. Post-dose peripheral collected blood samples were applied for the preparation of dry blood spots, dried matrix on paper discs, and peripheral plasma. Pharmacokinetic parameters determined were clearance (Cl), area under the curve (AUC), volume of distribution (Vd ), peak concentration (Cmax ), time of occurrence of peak concentration (Tmax ) and half-life time (T½ ). Observed pharmacokinetic values were not statistically (ANOVA) different compared to in literature reported values based on venous blood collection. The present pilot study demonstrated the feasibility of peripheral blood microsampling techniques in combination with quantitative liquid chromatography-high resolution mass spectrometry analysis for the determination of pharmacokinetics in clinical studies.

journal_name

Drug Test Anal

authors

Rincón JP,Meesters RJ

doi

10.1002/dta.1582

subject

Has Abstract

pub_date

2014-06-01 00:00:00

pages

568-77

issue

6

eissn

1942-7603

issn

1942-7611

journal_volume

6

pub_type

临床试验,杂志文章
  • Seven years of workplace drug testing in Italy: A systematic review and meta-analysis.

    abstract::In Italy, Workplace Drug Testing (WDT) has been compulsory by law for specific categories of workers since 2008, offering the opportunity to compare studies conducted within a single regulatory framework. The aims of this paper are to estimate the overall prevalence of WDT positivity (at screening survey) among Italia...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章,meta分析,评审

    doi:10.1002/dta.2189

    authors: Rosso GL,Montomoli C,Morini L,Candura SM

    更新日期:2017-06-01 00:00:00

  • PVC membrane sensor for potentiometric determination of iron (II) in some pharmaceutical formulations based on a new neutral ionophore.

    abstract::A novel poly (vinyl chloride) PVC membrane sensor for Fe(2+) ions is described. The sensor is based on the use of newly synthesized chiral 2,6-bis-(carboxamide methyl ester)pyridine derivative as neutral ionophore in plasticized PVC membrane. The sensor display a fast, stable and near-Nernstian response over a relativ...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.231

    authors: Abounassif MA,Al-Omar MA,Amr AG,Mostafa GA

    更新日期:2011-06-01 00:00:00

  • Steroid profile and IRMS analysis of musk administration for doping control.

    abstract::Musk, the dried secretion of the musk pod (sac) of adult male musk deer, has been used as traditional Chinese Medicine (TCM) in China and south-east Asian countries for thousands of years. Due to the anabolic steroid component in this TCM, musk preparations have been included in the list of medical products containing...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.2293

    authors: Wang J,He Y,Liu X,Yang Z,Yang W

    更新日期:2017-11-01 00:00:00

  • Synthesis and identification of deschloroketamine metabolites in rats' urine and a quantification method for deschloroketamine and metabolites in rats' serum and brain tissue using liquid chromatography tandem mass spectrometry.

    abstract::Deschloroketamine (2-(methylamino)-2-phenyl-cyclohexanone) is a ketamine analog belonging to a group of dissociative anesthetics, which have been distributed within the illicit market since 2015. However, it was also being sold as 'ketamine' misleading people to believe that they were getting genuine ketamine. Dissoci...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.2726

    authors: Hájková K,Jurásek B,Čejka J,Štefková K,Páleníček T,Sýkora D,Kuchař M

    更新日期:2020-03-01 00:00:00

  • The synthesis and characterization N-methyl-3-phenyl-norbornan-2-amine (Camfetamine™).

    abstract::N-Methyl-3-phenyl-norbornan-2-amine (N-methyl-3-phenylbicyclo[2.2.1]heptan-2-amine, Camfetamine(™) ) is available from a number of online legal highs/research chemicals' vendors. Although it was developed as an analeptic by Merck in the early 1960s, it was never commercialized. However, the Association of Independent ...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.411

    authors: Kavanagh P,Angelov D,O'Brien J,Power JD,McDermott SD,Talbot B,Fox J,O'Donnell C,Christie R

    更新日期:2013-04-01 00:00:00

  • Pharmacokinetics of betamethasone in plasma, urine, and synovial fluid following intra-articular administration to exercised thoroughbred horses.

    abstract::The use of corticosteroids, such as betamethasone, in performance horses is tightly regulated. The objective of the current study was to describe the plasma pharmacokinetics of betamethasone as well as time-related urine and synovial fluid concentrations following intra-articular administration to horses. Twelve racin...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.2170

    authors: Knych HK,Stanley SD,Harrison LM,Mckemie DS

    更新日期:2017-09-01 00:00:00

  • GC-MS and GC-IRD studies on brominated dimethoxyamphetamines: regioisomers related to 4-Br-2,5-DMA (DOB).

    abstract::A series of regioisomeric bromodimethoxyamphetamines have mass spectra essentially equivalent to the controlled drug substance 4-Br-2,5-dimethoxyamphetamine (4-Br-2,5-DMA; DOB); all have molecular weight of 274 and major fragment ions in their electron ionization mass spectra at m/z 44 and m/z 230/232. The trifluoroac...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.409

    authors: Maher HM,Awad T,DeRuiter J,Randall Clark C

    更新日期:2012-07-01 00:00:00

  • Inter-individual variation of the urinary steroid profiles in Swedish and Norwegian athletes.

    abstract::The steroidal module of the Athlete Biological Passport (ABP) aims to detect doping with endogenous steroids, e.g. testosterone (T), by longitudinally monitoring several biomarkers. These biomarkers are ratios combined into urinary concentrations of testosterone and metabolically related steroids. However, it is evide...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.2778

    authors: Mullen J,Baekken LV,Törmäkangas T,Ekström L,Ericsson M,Hullstein IR,Schulze JJ

    更新日期:2020-06-01 00:00:00

  • Drug residues in used syringes in Switzerland: A comparative study.

    abstract::Harm reduction services, including needle-exchange programmes, have been implemented in Switzerland for over 20 years. Their main aim is to lessen the negative social and/or physical consequences associated with illicit drug consumption and, therefore, improve prevention messages. To this end, knowledge of illicit dru...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.2335

    authors: Lefrançois E,Augsburger M,Esseiva P

    更新日期:2018-05-01 00:00:00

  • Head-twitch response in rodents induced by the hallucinogen 2,5-dimethoxy-4-iodoamphetamine: a comprehensive history, a re-evaluation of mechanisms, and its utility as a model.

    abstract::Two primary animal models persist for assessing hallucinogenic potential of novel compounds and for examining the pharmacological and neurobiological substrates underlying the actions of classical hallucinogens, the two-lever drug discrimination procedure and the drug-induced head-twitch response (HTR) in rodents. The...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章,评审

    doi:10.1002/dta.1333

    authors: Canal CE,Morgan D

    更新日期:2012-07-01 00:00:00

  • Recent instrumental progress in mass spectrometry: advancing resolution, accuracy, and speed of drug detection.

    abstract::Fast and unequivocal drug detection is of considerable importance in numerous fields of analytical chemistry, and today mass spectrometry-based approaches are often the method of choice due to their sensitive and specific nature. Mass spectrometry is in constant flux with innovations and thus supports the development ...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.344

    authors: Thevis M,Volmer DA

    更新日期:2012-03-01 00:00:00

  • Biomarker detection of rhGH doping: an excretion study.

    abstract::The purpose of this research is to validate the biomarker-based approach for the detection of doping with recombinant human growth hormone (rhGH) in sport. The GH-2000 project proposed an indirect method for the detection of exogenously administered growth hormone (GH) based on the measurement of the GH-dependent mark...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.1423

    authors: Jing J,Zhou X,He C,Zhang L,Yang S,Xu Y,Xie M,Yan Y,Su H,Wu M

    更新日期:2012-10-01 00:00:00

  • In vitro studies on flubromazolam metabolism and detection of its metabolites in authentic forensic samples.

    abstract::Flubromazolam is a triazole benzodiazepine with high potency and long-lasting central nervous system depressant effects; however, limited data about its pharmacokinetics are available. Here, we report in vitro studies of the human flubromazolam metabolism analyzed by liquid chromatography high-resolution mass spectrom...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.2146

    authors: Noble C,Mardal M,Bjerre Holm N,Stybe Johansen S,Linnet K

    更新日期:2017-08-01 00:00:00

  • Sensitive and selective spectrophotometric assay of gabapentin in capsules using sodium 1, 2-naphthoquinone-4-sulfonate.

    abstract::A simple, sensitive, and selective spectrophotometric method has been developed for the determination of gabapentin (GBP) in capsules. The method is based on the reaction of GBP and sodium 1,2-naphthoquinone-4-sulfonate (NQS) in the presence of Clark and Lubs buffer of pH 11 to form an orange-coloured product which wa...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.242

    authors: Abdulrahman SA,Basavaiah K

    更新日期:2011-10-01 00:00:00

  • Acute toxicity and withdrawal syndromes related to γ-hydroxybutyrate (GHB) and its analogues γ-butyrolactone (GBL) and 1,4-butanediol (1,4-BD).

    abstract::Gamma-hydroxybutyrate (GHB) has been used as a recreational drug since the 1990s and over the last few years there has been increasing use of its analogues gamma-butyrolactone (GBL) and to a lesser extent 1,4-butanediol (1,4BD). This review will summarize the literature on the pharmacology of these compounds; the patt...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章,评审

    doi:10.1002/dta.292

    authors: Wood DM,Brailsford AD,Dargan PI

    更新日期:2011-07-01 00:00:00

  • Voltammetric determination of promethazine hydrochloride at a multi-wall carbon nanotube modified glassy carbon electrode.

    abstract::A simple and sensitive method is described for voltammetric determination of promethazine hydrochloride (PMZ), a widely used phenothiazine drug, based on its electrochemical oxidation at a multi-wall carbon nanotube (MWCNT) modified glassy carbon electrode (GCE). Compared with bare GCE, the MWCNT-modified GCE exhibite...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.205

    authors: Xi X,Ming L,Liu J

    更新日期:2011-03-01 00:00:00

  • Evaluation of the performance of a tandem mass spectral library with mass spectral data extracted from literature.

    abstract::MSforID represents a database of tandem mass spectral data obtained from (quasi-)molecular ions produced by atmospheric pressure ionization methods. At the current stage of development the library contains 12 122 spectra of 1208 small (bio-)organic molecules. The present work was aimed to evaluate the performance of t...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.341

    authors: Würtinger P,Oberacher H

    更新日期:2012-03-01 00:00:00

  • Norcocaine and cocaethylene distribution patterns in hair samples from light, moderate, and heavy cocaine users.

    abstract::Even though hair analysis often seems to be the best choice for retrospective monitoring of cocaine intake, differentiating between incorporated cocaine and external contamination is widely debated. In this study we report results obtained in 90 hair samples from addicts. All samples were analyzed for cocaine, benzoyl...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.1903

    authors: Gambelunghe C,Rossi R,Aroni K,Gili A,Bacci M,Pascali V,Fucci N

    更新日期:2017-02-01 00:00:00

  • High sensitive analysis of steroids in doping control using gas chromatography/time-of-flight mass-spectrometry.

    abstract::The method of high sensitive gas chromatographic/time-of-flight mass-spectrometric (GC/TOF-MS) analysis of steroids was developed. Low-resolution TOF-MS instrument (with fast spectral acquisition rate) was used. This method is based on the formation of the silyl derivatives of steroids; exchange of the reagent mixture...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.221

    authors: Revelsky AI,Samokhin AS,Virus ED,Rodchenkov GM,Revelsky IA

    更新日期:2011-04-01 00:00:00

  • Transcriptomic biomarkers of altered erythropoiesis to detect autologous blood transfusion.

    abstract::Autologous blood transfusion is a powerful means of improving performance and remains one of the most challenging methods to detect. Recent investigations have identified 3 candidate reticulocytes genes whose expression was significantly influenced by blood transfusion. Using quantitative reverse transcription polymer...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.2240

    authors: Salamin O,Mignot J,Kuuranne T,Saugy M,Leuenberger N

    更新日期:2018-03-01 00:00:00

  • Development of a lateral flow dipstick for simultaneous and semi-quantitative analysis of dihydroartemisinin and piperaquine in an artemisinin combination therapy.

    abstract::Dihydroartemisinin (DHA) and piperaquine (PPQ) are two drugs used in an artemisinin-based combination therapy (ACT). The circulation of counterfeit antimalarial drugs demands the development of simple, point-of-care (POC) tests for monitoring drug quality. Here we aimed to design an antibody-based lateral flow dipstic...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.2656

    authors: Ning X,Tan G,Chen X,Wang M,Wang B,Cui L

    更新日期:2019-09-01 00:00:00

  • Screening of testosterone esters in human plasma.

    abstract::The detection of an intact ester of testosterone in plasma is leading towards unequivocal proof of the administration of exogenous testosterone. In the current study, a sensitive screening method for the detection of nine testosterone esters in human plasma was developed. By preparing oxime derivatives of intact testo...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.1560

    authors: Forsdahl G,Vatne HK,Geisendorfer T,Gmeiner G

    更新日期:2013-11-01 00:00:00

  • Preparation and analytical characterization of 1-(1-phenylcyclohexyl)piperidine (PCP) and 1-(1-phenylcyclohexyl)pyrrolidine (PCPy) analogues.

    abstract::Classic examples of psychoactive arylcycloalkylamines include ketamine and 1-(1-phenylcyclohexyl)piperidine (PCP) and many others serve as important structural templates for neuropharmacological research. The recent emergence of PCP analogues that can be obtained from internet retailers requires the implementation of ...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.1468

    authors: Wallach J,De Paoli G,Adejare A,Brandt SD

    更新日期:2014-07-01 00:00:00

  • Determination of acetylcholinesterase activity by the Ellman assay: a versatile tool for in vitro research on medical countermeasures against organophosphate poisoning.

    abstract::Inhibition of acetylcholinesterase (AChE) is the main mechanism of action of organophosphorus compounds (OP), and AChE reactivators (oximes) are at present the only causal therapeutic approach. Being the key target of OP toxicity, AChE may serve as a valuable tool for diagnosis of OP exposure as well as for the invest...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章,评审

    doi:10.1002/dta.337

    authors: Worek F,Eyer P,Thiermann H

    更新日期:2012-03-01 00:00:00

  • A review of analytical strategies for the detection of 'endogenous' steroid abuse in food production.

    abstract::Detection of the abuse of synthetic steroids in food production is nowadays relatively straightforward using modern techniques such as gas or liquid chromatography coupled to mass spectrometry (GC-MS/MS or LC-MS/MS, respectively). However, proving the abuse of 'endogenous' (or naturally occurring) steroids is more dif...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章,评审

    doi:10.1002/dta.1354

    authors: Scarth JP,Kay J,Teale P,Akre C,Le Bizec B,De Brabander HF,Vanhaecke L,Van Ginkel L,Points J

    更新日期:2012-08-01 00:00:00

  • GHB and synthetic cathinones: clinical effects and potential consequences.

    abstract::Designer drugs belong to a group of legally or illegally produced substances that are structurally and pharmacologically very similar to illicit drugs. In the past, designer drugs were often used during all-night dance parties, but they are now consumed in multiple settings from college bars to parks to private house ...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章,评审

    doi:10.1002/dta.210

    authors: Karila L,Reynaud M

    更新日期:2011-09-01 00:00:00

  • Fatal intoxication by 5F-ADB and diphenidine: Detection, quantification, and investigation of their main metabolic pathways in humans by LC/MS/MS and LC/Q-TOFMS.

    abstract::Despite the implementation of a new blanket scheduling system in 2013, new psychoactive substance (NPS) abuse remains a serious social concern in Japan. We present a fatal intoxication case involving 5F-ADB (methyl 2-[1-(5-fluoropentyl)-1H-indazole-3-carboxamido]-3,3-dimethylbutanoate) and diphenidine. Postmortem bloo...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.2215

    authors: Kusano M,Zaitsu K,Taki K,Hisatsune K,Nakajima J,Moriyasu T,Asano T,Hayashi Y,Tsuchihashi H,Ishii A

    更新日期:2018-02-01 00:00:00

  • Subject-based steroid profiling and the determination of novel biomarkers for DHT and DHEA misuse in sports.

    abstract::Doping with natural steroids can be detected by evaluating the urinary concentrations and ratios of several endogenous steroids. Since these biomarkers of steroid doping are known to present large inter-individual variations, monitoring of individual steroid profiles over time allows switching from population-based to...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.206

    authors: Van Renterghem P,Van Eenoo P,Sottas PE,Saugy M,Delbeke F

    更新日期:2010-11-01 00:00:00

  • Zebrafish (Danio rerio) water tank model for the investigation of drug metabolism: Progress, outlook, and challenges.

    abstract::Zebrafish (Danio rerio) water tank (ZWT) approach was investigated as an alternative model for metabolism studies based on six different experiments with four model compounds. Sibutramine was applied for the multivariate optimization of ZWT conditions, also for the comparison of the metabolism among ZWT, humans and mi...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.2523

    authors: Sardela VF,Anselmo CS,Nunes IKDC,Carneiro GRA,Dos Santos GRC,de Carvalho AR,Labanca BJ,Silva Oliveira D,Ribeiro WD,de Araujo ALD,Padilha MC,de Lima CKF,de Sousa VP,de Aquino Neto FR,Gualberto Pereira HM

    更新日期:2018-11-01 00:00:00

  • Mildronate (Meldonium) in professional sports - monitoring doping control urine samples using hydrophilic interaction liquid chromatography - high resolution/high accuracy mass spectrometry.

    abstract::To date, substances such as Mildronate (Meldonium) are not on the radar of anti-doping laboratories as the compound is not explicitly classified as prohibited. However, the anti-ischemic drug Mildronate demonstrates an increase in endurance performance of athletes, improved rehabilitation after exercise, protection ag...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章

    doi:10.1002/dta.1788

    authors: Görgens C,Guddat S,Dib J,Geyer H,Schänzer W,Thevis M

    更新日期:2015-11-01 00:00:00