Abstract:
:We recently published two protocols for the detection of Sotatercept (ACE-011, ACVR2A-Fc) and Luspatercept (ACE-536, ACVR2B-Fc) in human serum. Both methods used covalently immobilized antibodies on agarose beads for immunoprecipitation and SAR-PAGE/Western blotting for detection. Disadvantages were the relatively high amount of antibody required per sample (10 μg) and the need of a secondary antibody for the final detection. The updated protocols overcome these limitations by antigen-antibody complex formation in solution followed by capture of the complex with anti-antibody-coated magnetic beads. They also omit the secondary antibody incubation step by usage of biotinylated primary antibodies, which can be directly incubated with streptavidin-HRP. Thus, the new protocols are faster, simpler, and cheaper and offer comparable sensitivities.
journal_name
Drug Test Analjournal_title
Drug testing and analysisauthors
Reichel C,Gmeiner G,Walpurgis K,Thevis Mdoi
10.1002/dta.2500subject
Has Abstractpub_date
2018-11-01 00:00:00pages
1708-1713issue
11-12eissn
1942-7603issn
1942-7611journal_volume
10pub_type
杂志文章abstract::A simple and highly sensitive method that involves miniaturized hollow fibre assisted liquid-phase microextraction with gas chromatography-flame ionization detector was developed for the determination of trace concentration of sufentanil and alfentanil in biological samples. These drugs were extracted from 5 ml of aqu...
journal_title:Drug testing and analysis
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journal_title:Drug testing and analysis
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journal_title:Drug testing and analysis
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journal_title:Drug testing and analysis
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journal_title:Drug testing and analysis
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journal_title:Drug testing and analysis
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journal_title:Drug testing and analysis
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journal_title:Drug testing and analysis
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journal_title:Drug testing and analysis
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journal_title:Drug testing and analysis
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journal_title:Drug testing and analysis
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journal_title:Drug testing and analysis
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journal_title:Drug testing and analysis
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journal_title:Drug testing and analysis
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journal_title:Drug testing and analysis
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journal_title:Drug testing and analysis
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journal_title:Drug testing and analysis
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journal_title:Drug testing and analysis
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journal_title:Drug testing and analysis
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