Abstract:
:The determination of protein- or peptide-based performance-enhancing pharmaceuticals has gained increasing attention in sports drug testing in recent decades. Proteases possess a high potential for use in the manipulation of regular urine specimens, interfering with established doping control procedures for proteins or peptides. Hence, the present approach was developed to identify degradation products directly from proteases with exogenous origin in urine samples by means of SDS-PAGE and capillary liquid chromatography-Orbitrap (tandem) mass spectrometry. The mass spectrometric identification was accomplished by database search considering the accurate monoisotopic precursor mass with data dependent MS/MS analysis and no setting for specific cleavage site(s) or enzyme(s). The main characteristics of the method were shown in an exemplary way for the proteases Papain, Bacillolysin, Trypsin and Subtilisin with sufficient results for specificity, limit of detection, working range, precision and recovery after incubation. All experiments were performed with fortified urine samples under consideration of assumed realistic conditions for adulteration by cheating sportsmen.
journal_name
Drug Test Analjournal_title
Drug testing and analysisauthors
Thomas A,Kohler M,Walpurgis K,Schänzer W,Thevis Mdoi
10.1002/dta.20subject
Has Abstractpub_date
2009-02-01 00:00:00pages
81-6issue
2eissn
1942-7603issn
1942-7611journal_volume
1pub_type
杂志文章abstract::The abuse of stanozolol is quite widespread in Indian sport. Its analysis is challenging and this has led to the development of new methods to improve its detection. A method was developed and validated for the detection of the three main monohydroxylated metabolites of stanozolol. The excretion profile of these metab...
journal_title:Drug testing and analysis
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journal_title:Drug testing and analysis
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pub_type: 杂志文章,meta分析,评审
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journal_title:Drug testing and analysis
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journal_title:Drug testing and analysis
pub_type: 杂志文章
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更新日期:2014-07-01 00:00:00
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journal_title:Drug testing and analysis
pub_type: 杂志文章
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更新日期:2019-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2017-06-01 00:00:00
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