Abstract:
:The aim of this study was to investigate the effect of Morin on the pharmacokinetics of Piracetam in rats, in vitro enzyme kinetics and metabolic stability (high throughput) studies using human liver microsomes in UPLC. For pharmacokinetics studies, male Wistar rats were pretreated with Morin (10 mg/kg) for one week and on the last day, a single dose of Piracetam (50 mg/kg) was given orally. In another group, both Morin and Piracetam were co-administered to evaluate the acute effect of Morin on Piracetam. The control group received oral distilled water for one week and administered with Piracetam on the last day. As Morin is an inhibitor of P- Glycoprotein (P-gp) and CYP 3A, it was anticipated to improve the bioavailability of Piracetam. Amazingly, relative to control, the areas under the concentration time curve and peak plasma concentration of Piracetam were 1.50- and 1.45-fold, respectively, greater in the Morin-pretreated group. However, co-administration of Morin had no significant effect on these parameters. Apart from the aforementioned merits, the results of this study are further confirmed by clinical trials; Piracetam dosages should be adjusted to avoid potential drug interaction when Piracetam is used clinically in combination with Morin and Morin-containing dietary supplements. The in vitro enzyme kinetics were performed to determined km, Vmax & CLins . The in vitro metabolic stability executed for the estimation of metabolic rate constant and half-life of Piracetam. These studies also extrapolate to in vivo intrinsic hepatic clearance (Clint, in vivo ) from in vitro intrinsic hepatic clearance (CLint, in vitro ).
journal_name
Drug Test Analjournal_title
Drug testing and analysisauthors
Sahu K,Shaharyar M,Siddiqui AAdoi
10.1002/dta.1382subject
Has Abstractpub_date
2013-07-01 00:00:00pages
581-8issue
7eissn
1942-7603issn
1942-7611journal_volume
5pub_type
杂志文章abstract::In a preliminary test, four single hairs of a drug abuser were analyzed for the presence of drugs by MALDI Fourier transform mass spectrometry (MALDI-FTMS). Washed hair strains were directly fixed on a sample plate using pressure stable, double-sided adhesive tape; α-cyano-4-hydroxycinnamic acid matrix was manually sp...
journal_title:Drug testing and analysis
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journal_title:Drug testing and analysis
pub_type: 杂志文章
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journal_title:Drug testing and analysis
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journal_title:Drug testing and analysis
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journal_title:Drug testing and analysis
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journal_title:Drug testing and analysis
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pub_type: 历史文章,杂志文章
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journal_title:Drug testing and analysis
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journal_title:Drug testing and analysis
pub_type: 杂志文章
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journal_title:Drug testing and analysis
pub_type: 杂志文章
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journal_title:Drug testing and analysis
pub_type: 杂志文章
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journal_title:Drug testing and analysis
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journal_title:Drug testing and analysis
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