Pharmacokinetics of betamethasone in plasma, urine, and synovial fluid following intra-articular administration to exercised thoroughbred horses.


:The use of corticosteroids, such as betamethasone, in performance horses is tightly regulated. The objective of the current study was to describe the plasma pharmacokinetics of betamethasone as well as time-related urine and synovial fluid concentrations following intra-articular administration to horses. Twelve racing-fit adult Thoroughbred horses received a single intra-articular administration (9 mg) of a betamethasone sodium phosphate and betamethasone acetate injectable suspension into the right antebrachiocarpal joint. Blood, urine, and synovial fluid samples were collected prior to and at various times up to 21 days post drug administration. All samples were analyzed using tandem liquid chromatography-mass spectrometry. Plasma data were analyzed using compartmental pharmacokinetic modeling. Maximum measured plasma betamethasone concentrations were 3.97 ± 0.23 ng/mL at 1.45 ± 0.20 h. The plasma elimination half-life was 7.48 ± 0.39 h. Betamethasone concentrations were below the limit of detection in all horses by 96 h and 7 days in plasma and urine, respectively. Betamethasone fell below the limit of detection in the right antebrachiocarpal joint between 14 and 21 days. Results of this study provide information that can be used to regulate the use of intra-articular betamethasone in the horse. Copyright © 2017 John Wiley & Sons, Ltd.


Drug Test Anal


Knych HK,Stanley SD,Harrison LM,Mckemie DS




Has Abstract


2017-09-01 00:00:00












  • Simultaneous quantification of digoxin, digitoxin, and their metabolites in serum using high performance liquid chromatography-tandem mass spectrometry.

    abstract::The study describes an LC-MS/MS method for simultaneous quantification of the cardiac glycosidic drugs digoxin and digitoxin and several of their metabolites. The assay represents a useful reference method for immunoassay-based tests, which are easily biased by the presence of metabolites of the target analytes or str...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章


    authors: Bylda C,Thiele R,Kobold U,Volmer DA

    更新日期:2015-10-01 00:00:00

  • Electroanalysis of trimethoprim on metalloporphyrin incorporated glassy carbon electrode.

    abstract::Trimethoprim (TMP) is a bacteriostatic antibiotic mainly used in the prophylaxis and treatment of urinary tract infections. It belongs to the class of chemotherapeutic agents known as dihydrofolate reductase inhibitors. Its use is associated with idiosyncratic reactions, including liver toxicity and agranulocytosis. I...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章


    authors: Rajith L,Kumar KG

    更新日期:2010-09-01 00:00:00

  • Effect of single doses of methoxypolyethylene glycol-epoetin beta (CERA, Mircera™) and epoetin delta (Dynepo™) on isoelectric erythropoietin profiles and haematological parameters.

    abstract::Erythropoietin (EPO) has been misused in sports for many years due to its performance-enhancing effect. In the last decade, detection of abuse has been possible with isoelectric focusing (IEF) based on the different isoform profiles of endogenous and recombinant EPO. The release of new EPOs on the market, such as the ...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章


    authors: Dehnes Y,Hemmersbach P

    更新日期:2011-05-01 00:00:00

  • Elimination profile of triamcinolone in urine following oral administration.

    abstract::Triamcinolone (T) is a glucocorticoid commonly used to relieve inflammation and treat arthritis, severe allergies, and asthma; however, it is banned by the World Anti-Doping Agency in competition for athletes when administered orally, intravenously, intramuscularly, or rectally. The minimum required performance limit ...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章


    authors: Chen TT,Tseng YC,Huang TY,Chang-Chien GP,Hsu MC

    更新日期:2018-05-01 00:00:00

  • Determination of cocaine, cocaine metabolites and cannabinoids in single hairs by MALDI Fourier transform mass spectrometry--preliminary results.

    abstract::In a preliminary test, four single hairs of a drug abuser were analyzed for the presence of drugs by MALDI Fourier transform mass spectrometry (MALDI-FTMS). Washed hair strains were directly fixed on a sample plate using pressure stable, double-sided adhesive tape; α-cyano-4-hydroxycinnamic acid matrix was manually sp...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章


    authors: Musshoff F,Arrey T,Strupat K

    更新日期:2013-05-01 00:00:00

  • Synthesis and characterization of 5-methoxy-2-methyl-N,N-dialkylated tryptamines.

    abstract::The absence of reference material is a commonly experienced difficulty among medical and forensic professionals tasked with identifying new psychoactive substances that are encountered for the first time. The identification of newly emerging substances lies at the heart of forensic and clinical analysis, and a proacti...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章


    authors: Brandt SD,Tearavarich R,Dempster N,Cozzi NV,Daley PF

    更新日期:2012-01-01 00:00:00

  • An overview of gamma-hydroxybutyric acid: pharmacodynamics, pharmacokinetics, toxic effects, addiction, analytical methods, and interpretation of results.

    abstract::Abuse of gamma-hydroxybutyric acid (GHB) has been known since the early 1990's, but is not as widespread as the consumption of other illegal drugs. However, the number of severe intoxications with fatal outcomes is comparatively high; not the least of which is brought about by the consumption of the currently legal pr...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章,评审


    authors: Andresen H,Aydin BE,Mueller A,Iwersen-Bergmann S

    更新日期:2011-09-01 00:00:00

  • Antibiotic use and resistance in animals: Belgian initiatives.

    abstract::The widespread use of antibiotics in animals is causing concerns about the growing risk for development and the spread of antibiotic-resistant bacteria. Antibiotic consumption is higher in animals than in humans as reported in a joint publication of EFSA (European Food Safety Agency), ECDC (European Centre for Disease...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章


    authors: Daeseleire E,De Graef E,Rasschaert G,De Mulder T,Van den Meersche T,Van Coillie E,Dewulf J,Heyndrickx M

    更新日期:2016-05-01 00:00:00

  • Monitoring drug markets in the Internet age and the evolution of drug monitoring systems in Australia.

    abstract::In Australia, drug monitoring systems have been in place for more than a decade allowing for the measurement of ongoing trends in drug use and the detection of new drugs. The Drug Trends Unit at the National Drug and Alcohol Research Centre monitors drugs through four separate systems. The Illicit Drug Reporting Syste...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章,评审


    authors: Burns L,Roxburgh A,Bruno R,Van Buskirk J

    更新日期:2014-07-01 00:00:00

  • Steroid profile and IRMS analysis of musk administration for doping control.

    abstract::Musk, the dried secretion of the musk pod (sac) of adult male musk deer, has been used as traditional Chinese Medicine (TCM) in China and south-east Asian countries for thousands of years. Due to the anabolic steroid component in this TCM, musk preparations have been included in the list of medical products containing...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章


    authors: Wang J,He Y,Liu X,Yang Z,Yang W

    更新日期:2017-11-01 00:00:00

  • Guidelines for European workplace drug and alcohol testing in hair.

    abstract::Drug and alcohol abuse is a concern for many European companies, especially those having safety-critical jobs. It is not uncommon for European companies to establish a drug policy with little or no provision for drug testing. The European Union (EU) has launched a number of initiatives in its fight against drugs. Ther...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章


    authors: Agius R,Kintz P,European Workplace Drug Testing Society.

    更新日期:2010-08-01 00:00:00

  • In vivo metabolism of the designer anabolic steroid hemapolin in the thoroughbred horse.

    abstract::Hemapolin (2α,3α-epithio-17α-methyl-5α-androstan-17β-ol) is a designer steroid that is an ingredient in several "dietary" and "nutritional" supplements available online. As an unusual chemical modification to the steroid A-ring could allow this compound to pass through antidoping screens undetected, the metabolism of ...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章


    authors: Waller CC,Weththasinghe SA,McClure L,Cawley AT,Suann C,Suann E,Sutherland E,Cooper E,Heather A,McLeod MD

    更新日期:2020-06-01 00:00:00

  • Evaluation of peripheral blood microsampling techniques in combination with liquid chromatography-high resolution mass spectrometry for the determination of drug pharmacokinetics in clinical studies.

    abstract::New bioanalytical assays were developed, validated, and applied in a clinical study for quantitative measurement of acetaminophen concentrations in blood and plasma samples. Furthermore, after validation, the bioanalytical assays were used for determination of pharmacokinetics within a group of six healthy male human ...

    journal_title:Drug testing and analysis

    pub_type: 临床试验,杂志文章


    authors: Rincón JP,Meesters RJ

    更新日期:2014-06-01 00:00:00

  • Zebrafish (Danio rerio) water tank model for the investigation of drug metabolism: Progress, outlook, and challenges.

    abstract::Zebrafish (Danio rerio) water tank (ZWT) approach was investigated as an alternative model for metabolism studies based on six different experiments with four model compounds. Sibutramine was applied for the multivariate optimization of ZWT conditions, also for the comparison of the metabolism among ZWT, humans and mi...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章


    authors: Sardela VF,Anselmo CS,Nunes IKDC,Carneiro GRA,Dos Santos GRC,de Carvalho AR,Labanca BJ,Silva Oliveira D,Ribeiro WD,de Araujo ALD,Padilha MC,de Lima CKF,de Sousa VP,de Aquino Neto FR,Gualberto Pereira HM

    更新日期:2018-11-01 00:00:00

  • Stability-indicating chemometric methods for the determination of pyritinol dihydrochloride.

    abstract::Three multivariate calibration methods, including classical least square with nonzero intercept (CLS), principal component regression (PCR) and partial least square (PLS), have been used for the determination of pyritinol dihydrochloride in the presence of its degradation product. The CLS, PCR and PLS techniques are u...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章


    authors: El-Sayed MA,Abdul-Azim Mohammad M

    更新日期:2009-05-01 00:00:00

  • Detection of urinary metabolites of AM-2201 and UR-144, two novel synthetic cannabinoids.

    abstract::Synthetic cannabinoids are the psychotropic compounds frequently identified as active components of smoking mixtures easily available via the Internet in several countries. These herbal blends have become extremely popular as a legal alternative to cannabis-based products and are difficult to detect by regular drug te...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章


    authors: Sobolevsky T,Prasolov I,Rodchenkov G

    更新日期:2012-10-01 00:00:00

  • Infrared attenuated total reflection (IR-ATR) spectroscopy for detecting drugs in human saliva.

    abstract::The consumption of drugs is of great concern worldwide. Various drug tests for humans have been developed but there is no compact and easy-to-use test device available yet for direct semi-quantitative drug testing in the field. We suggest using attenuated total reflection (ATR) infrared spectroscopy as a sensing metho...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章


    authors: Hans KM,Müller S,Sigrist MW

    更新日期:2012-06-01 00:00:00

  • Updated protocols for the detection of Sotatercept and Luspatercept in human serum.

    abstract::We recently published two protocols for the detection of Sotatercept (ACE-011, ACVR2A-Fc) and Luspatercept (ACE-536, ACVR2B-Fc) in human serum. Both methods used covalently immobilized antibodies on agarose beads for immunoprecipitation and SAR-PAGE/Western blotting for detection. Disadvantages were the relatively hig...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章


    authors: Reichel C,Gmeiner G,Walpurgis K,Thevis M

    更新日期:2018-11-01 00:00:00

  • Assessement of the pharmacokinetics, tissue distribution and excretion studies of a novel antiplatelet agent S007-867, following administration to rats.

    abstract::S007-867 is a promising novel antiplatelet agent with better efficacy and lesser bleeding risk than existing agents. The present study investigated the absorption, tissue distribution, and excretion of S007-867 in rat model for further advancement of the molecule. A simple and robust ultra fast liquid chromatography-t...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章


    authors: Chandasana H,Chhonker YS,Laxman TS,Prasad YD,K S AK,Dikshit DK,Bhatta RS

    更新日期:2016-07-01 00:00:00

  • Prediction of polar surface area of drug molecules: a QSPR approach.

    abstract::A quantitative structure-property relationship (QSPR) study based on an artificial neural network (ANN) was carried out for the prediction of the microemulsion liquid chromatography polar surface area (PSA) of a set of 32 drug compounds. The genetic algorithm-kernel partial least squares (GA-KPLS) method was used as a...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章


    authors: Noorizadeh H,Farmany A,Noorizadeh M,Kohzadi M

    更新日期:2013-04-01 00:00:00

  • Drug concentrations in post-mortem femoral blood compared with therapeutic concentrations in plasma.

    abstract::Therapeutic drug concentrations measured in plasma are of limited value as reference intervals for interpretation in post-mortem (PM) toxicology. In this study, drug concentration distributions were studied in PM femoral venous blood from 57 903 Finnish autopsy cases representing all causes of death during an 11-year ...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章


    authors: Launiainen T,Ojanperä I

    更新日期:2014-04-01 00:00:00

  • Sensitive and selective spectrophotometric assay of gabapentin in capsules using sodium 1, 2-naphthoquinone-4-sulfonate.

    abstract::A simple, sensitive, and selective spectrophotometric method has been developed for the determination of gabapentin (GBP) in capsules. The method is based on the reaction of GBP and sodium 1,2-naphthoquinone-4-sulfonate (NQS) in the presence of Clark and Lubs buffer of pH 11 to form an orange-coloured product which wa...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章


    authors: Abdulrahman SA,Basavaiah K

    更新日期:2011-10-01 00:00:00

  • Detection of iso-α-acids to confirm beer consumption in postmortem specimens.

    abstract::Iso-α-acids (IAAs) can be used as markers for the consumption of beer. Postmortem specimens from a range of coronial cases were analyzed for IAAs in order to determine the prevalence of beer consumption and any correlation to blood alcohol concentrations (BAC). A total of 130 cases were included in this study includin...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章


    authors: Rodda LN,Gerostamoulos D,Drummer OH

    更新日期:2015-01-01 00:00:00

  • Acute toxicity and withdrawal syndromes related to γ-hydroxybutyrate (GHB) and its analogues γ-butyrolactone (GBL) and 1,4-butanediol (1,4-BD).

    abstract::Gamma-hydroxybutyrate (GHB) has been used as a recreational drug since the 1990s and over the last few years there has been increasing use of its analogues gamma-butyrolactone (GBL) and to a lesser extent 1,4-butanediol (1,4BD). This review will summarize the literature on the pharmacology of these compounds; the patt...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章,评审


    authors: Wood DM,Brailsford AD,Dargan PI

    更新日期:2011-07-01 00:00:00

  • Identification of porcine relaxin in plasma by liquid chromatography-high resolution mass spectrometry.

    abstract::Relaxin (RLX) has demonstrated diverse pharmacological effects in various scientific and clinical studies. The emergence of porcine relaxin (pRLX) has raised concerns on the doping potential of pRLX. There have also been speculations in the horseracing industry on its covert use. To control the abuse of pRLX in equine...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章


    authors: Wong ASY,Ho ENM,Kwok WH,Leung GNW,Shen Y,Qi RZ,Yue SK,Wan TSM

    更新日期:2017-09-01 00:00:00

  • Tentative identification of the metabolites of (1-(cyclohexylmethyl)-1H-indol-3-yl)-(2,2,3,3-tetramethylcyclopropyl)methanone, and the product of its thermal degradation, by in vitro and in vivo methods.

    abstract::Synthetic cannabinoids (SCs), mimicking the psychoactive effects of cannabis, consist of a vast array of structurally diverse compounds. A novel compound belonging to the SC family, (1-(cyclohexylmethyl)-1H-indol-3-yl)-(2,2,3,3-tetramethylcyclopropyl)methanone (named TMCP-CHM in this article) contains a cyclopropane r...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章


    authors: Grigoryev A,Kavanagh P,Labutin A,Pechnikov A,Dowling G,Shevyrin V,Krupina N

    更新日期:2019-09-01 00:00:00

  • High sensitive analysis of steroids in doping control using gas chromatography/time-of-flight mass-spectrometry.

    abstract::The method of high sensitive gas chromatographic/time-of-flight mass-spectrometric (GC/TOF-MS) analysis of steroids was developed. Low-resolution TOF-MS instrument (with fast spectral acquisition rate) was used. This method is based on the formation of the silyl derivatives of steroids; exchange of the reagent mixture...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章


    authors: Revelsky AI,Samokhin AS,Virus ED,Rodchenkov GM,Revelsky IA

    更新日期:2011-04-01 00:00:00

  • Elephant in the room? The methodological implications for public health research of performance-enhancing drugs derived from the illicit market.

    abstract::'All scientific work is incomplete-whether it be observational or experimental. All scientific work is liable to be upset or modified by advancing knowledge. That does not confer upon us a freedom to ignore the knowledge we already have, or to postpone the action that it appears to demand at a given time.' ...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章


    authors: Evans-Brown M,Kimergård A,McVeigh J

    更新日期:2009-07-01 00:00:00

  • Study of the in vitro and in vivo metabolism of the tryptamine 5-MeO-MiPT using human liver microsomes and real case samples.

    abstract::The synthetic tryptamine 5-methoxy-N-methyl-N-isopropyltryptamine (5-MeO-MiPT) has recently been abused as a hallucinogenic drug in Germany and Switzerland. This study presents a case of 5-MeO-MiPT intoxication and the structural elucidation of metabolites in pooled human liver microsomes (pHLM), blood, and urine. Mic...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章


    authors: Grafinger KE,Hädener M,König S,Weinmann W

    更新日期:2018-03-01 00:00:00

  • Sulfate metabolites as alternative markers for the detection of 4-chlorometandienone misuse in doping control.

    abstract::Sulfate metabolites have been described as long-term metabolites for some anabolic androgenic steroids (AAS). 4-chlorometandienone (4Cl-MTD) is one of the most frequently detected AAS in sports drug testing and it is commonly detected by monitoring metabolites excreted free or conjugated with glucuronic acid. Sulfatio...

    journal_title:Drug testing and analysis

    pub_type: 杂志文章


    authors: Balcells G,Gómez C,Garrostas L,Pozo ÓJ,Ventura R

    更新日期:2017-07-01 00:00:00