Abstract:
AIMS:To define the possible effect of diabetic conditions on megakaryocytes, the long-know precursors of platelets and lately characterized modulator of hematopoietic stem quiescence-activation transition. METHODS:Megakaryoblastic MEG-01 cell culture and TPO/SCF/IL-3-induced differentiation of human umbilical blood mononuclear cells toward megakaryocytes were used to test effects of glycated bovine serum albumin (BSA-AGEs). The ob/ob mice and streptozotocin-treated mice were used as models of hyperglycemia. MTT was used to measure cell proliferation, FACS for surface marker and cell cycle, and RT-qPCR for the expression of interested genes. Megakaryocytes at different stages in marrow smear were checked under microscope. RESULTS:When added in MEG-01 cultures at 200 μg/ml, BSA-AGEs increased proliferation of cells and enhanced mRNA expression of RAGE, VEGFα and PF4 in the cells. None of cell cycle distribution, PMA-induced platelet-like particles production, expression of GATA1/NF-E2/PU-1/IL-6/OPG/PDGF in MEG-01 cells nor TPO/SCF/IL-3 induced umbilical cord blood cells differentiation into megakaryocyte was affected by BSA-AGEs. In the ob/ob diabetic mice, MKs percentages in marrow cells and platelets in peripheral blood were significantly increased compared with control mice. In streptozotocin-induced diabetic mice, however, MKs percentage in marrow cells was decreased though peripheral platelet counts were not altered. Gene expression assay showed that the change in MKs in these two diabetic conditions might be explained by the alteration of GATA1 and NF-E2 expression, respectively. CONCLUSIONS:Diabetic condition in animals might exert its influence on hematopoiesis via megakaryocytes-the newly identified modulator of hematopoietic stem cells in bone marrow.
journal_name
Acta Diabetoljournal_title
Acta diabetologicaauthors
Wang B,Yu J,Wang T,Shen Y,Lin D,Xu X,Wang Ydoi
10.1007/s00592-018-1109-zsubject
Has Abstractpub_date
2018-05-01 00:00:00pages
419-427issue
5eissn
0940-5429issn
1432-5233pii
10.1007/s00592-018-1109-zjournal_volume
55pub_type
杂志文章abstract::Islet autotransplantation (IAT) is performed at the time of total pancreatectomy (TP) to prevent or minimize post-surgical diabetes. Corticosteroids induce insulin resistance and present a risk to islet autografts, through glucotoxicity and increased metabolic demand on a marginal islet mass. We present four IAT recip...
journal_title:Acta diabetologica
pub_type: 杂志文章
doi:10.1007/s00592-011-0315-8
更新日期:2014-02-01 00:00:00
abstract::The assessment of body composition and energy requirements is important for the nutrition of healthy and pathological states such as gastrointestinal disorders, which are known to be associated with abnormalities in body composition among persons with malnutrition and malabsorption. Careful monitoring of body composit...
journal_title:Acta diabetologica
pub_type: 杂志文章,评审
doi:10.1007/s00592-003-0053-7
更新日期:2003-10-01 00:00:00
abstract::MicroRNAs (miRNAs) play a crucial role in the pathogenesis of type 2 diabetes (T2D); they regulate several metabolic pathways including insulin secretion, glucose homeostasis, so their potential as biomarkers of diagnosis and prognosis has became increasingly appreciated. In this study, we explore serum miRNA profiles...
journal_title:Acta diabetologica
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doi:10.1007/s00592-014-0617-8
更新日期:2014-10-01 00:00:00
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journal_title:Acta diabetologica
pub_type: 杂志文章,评审
doi:10.1007/s00592-018-1211-2
更新日期:2018-11-01 00:00:00
abstract:AIMS:Genetic polymorphisms may contribute to platelet reactivity in diabetic patients; however, the information on their influence on long-term antiplatelet therapy is lacking. Our aim was to evaluate the role of previously described genetic variants and platelet reactivity on risk of all-cause mortality and cardiovasc...
journal_title:Acta diabetologica
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1007/s00592-016-0945-y
更新日期:2017-04-01 00:00:00
abstract:AIMS:To determine whether blood glucose test strip (BGTS) utilization in patients with type 2 diabetes (T2D) is associated with the type of diabetes therapy, classified according to hypoglycemic risk. METHODS:A retrospective, longitudinal (2006-2012) study of Canadian private drug plans (PDP) and Ontario Public Drug P...
journal_title:Acta diabetologica
pub_type: 杂志文章
doi:10.1007/s00592-015-0823-z
更新日期:2016-06-01 00:00:00
abstract::Although reactive oxygen species (ROS) contribute to glucose intolerance induced by the renin-angiotensin system (RAS) is well documented, the role of the newly discovered pathway of RAS, angiotensin (Ang)-(1-7)/Mas axis, in this process remains unknown. Here, we examined the effect of Ang-(1-7) on oxidative stress an...
journal_title:Acta diabetologica
pub_type: 杂志文章
doi:10.1007/s00592-011-0348-z
更新日期:2012-08-01 00:00:00
abstract::Consistent with the postulated role of oxidative stress in the etiology of late diabetic complications, pharmacological interventions based on biological antioxidants have been suggested. The aim of the present study was to investigate the effect of dietary supplementation with the pyridoindole antioxidant stobadine o...
journal_title:Acta diabetologica
pub_type: 杂志文章
doi:10.1007/s005920070012
更新日期:2000-01-01 00:00:00
abstract:AIMS:To evaluate feasibility and effectiveness on short-term blood glucose control of using glycaemic load counting (GLC) versus carbohydrate counting (CC) for prandial insulin dosing in patients with type 1 diabetes (T1D). METHODS:Nine T1D patients on insulin pump, aged 26-58 years, HbA1c 7.7 ± 0.8 % (61 ± 8.7 mmol/m...
journal_title:Acta diabetologica
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00592-015-0716-1
更新日期:2015-10-01 00:00:00
abstract::The objective of this study is to explore the mechanism of oxidative stress induced by intermittent high glucose in porcine iliac endothelial cells (PIECs). The PIECs were exposed to intermittent or constant high glucose for 3 or 6 days, and the mean fluorescent intensity (MFI) was measured via intracellular reactive ...
journal_title:Acta diabetologica
pub_type: 杂志文章
doi:10.1007/s00592-009-0140-5
更新日期:2010-12-01 00:00:00
abstract::A literature review of 37 papers cited by MEDLINE between 1986 and April 1996 under the terms "diabetes", "patient education" and "randomized" was carried out. The articles were analysed on the basis of a check list (Educational Procedure Check List), which contains 27 items grouped into 8 areas: study objectives, edu...
journal_title:Acta diabetologica
pub_type: 杂志文章,评审
doi:10.1007/s005920050133
更新日期:1998-12-01 00:00:00
abstract:AIMS:Hypoglycaemia represents a critical burden with clinical and social consequences in the management of diabetes. Serum uric acid (SUA) has been associated with cardiovascular diseases (CVD), but no conclusive findings are available nowadays in patients suffering from hypoglycaemia. We investigated whether SUA level...
journal_title:Acta diabetologica
pub_type: 杂志文章
doi:10.1007/s00592-017-1094-7
更新日期:2018-04-01 00:00:00
abstract::Microalbuminuria in diabetic patients is associated with an increased cardiovascular risk which is not completely explained by an excess of conventional cardiovascular risk factors. A depression of physiologic inhibitors of blood coagulation could contribute to a thrombophilic state and to cardiovascular complications...
journal_title:Acta diabetologica
pub_type: 杂志文章
doi:10.1007/BF00569567
更新日期:1995-06-01 00:00:00
abstract:AIMS:Observational studies indicated that resting heart rate (RHR) was associated with diabetes mellitus (DM) risk; however, it remains unclear whether the association between RHR and DM is causal. We aimed to examine whether there was causal association of RHR with DM risk. METHODS:A prospective study including 16,20...
journal_title:Acta diabetologica
pub_type: 杂志文章
doi:10.1007/s00592-019-01344-3
更新日期:2019-09-01 00:00:00
abstract::The purification of large numbers of human pancreatic islets remains one of the limiting factors in islet transplantation. This paper describes and validates a method for accurately and reproducibly determining the density of islets and exocrine tissue in pancreatic digest on the basis of their isopycnic distribution ...
journal_title:Acta diabetologica
pub_type: 杂志文章
doi:10.1007/BF00572864
更新日期:1993-01-01 00:00:00
abstract::Patients with diabetes represent an increasing proportion of end-stage renal disease (ESRD) patients. Cardiovascular risk, already formidable among patients on dialysis, is significantly higher among those who also have diabetes. Diabetic ESRD patients are not only at higher risk of ischaemic events, but are also subj...
journal_title:Acta diabetologica
pub_type: 杂志文章,评审
doi:10.1007/s005920200003
更新日期:2002-04-01 00:00:00
abstract::Obesity, insulin resistance (IR), and progressive decline in pancreatic β-cell function are major features of type 2 diabetes mellitus (T2DM). Altered adipokines contribute to obesity-induced IR. Hence understanding of adipokines' relation to obesity and glycemic control could be useful to improve disease outcomes. We...
journal_title:Acta diabetologica
pub_type: 杂志文章
doi:10.1007/s00592-012-0373-6
更新日期:2013-10-01 00:00:00
abstract::Diabetes mellitus, the most common serious metabolic disorder, is characterized by functional and structural changes in the peripheral and central nervous systems. Glial cells provide structural and metabolic support for retinal neurons. During diabetes, one of the early pathogenic events is retinal glial reactivity. ...
journal_title:Acta diabetologica
pub_type: 杂志文章
doi:10.1007/s00592-004-0155-x
更新日期:2004-09-01 00:00:00
abstract::The objective of the study was to determine the prevalence of known diabetes among elderly subjects receiving nursing care in a north Norwegian population, to screen for new cases using HbA1c measurement and to evaluate the quality of care for those with diabetes. Men and women, with and without known diabetes, above ...
journal_title:Acta diabetologica
pub_type: 杂志文章
doi:10.1007/s00592-006-0212-8
更新日期:2006-08-01 00:00:00
abstract:AIMS:Increasing evidence suggests a potential role of circulating miRNAs as clinical biomarkers, and loss of miRNA-126 has been proposed as a predictor of type 2 diabetes onset. However, a systematic analysis of circulating miRNAs in type 1 diabetic patients with micro-/macrovascular complications has not yet been perf...
journal_title:Acta diabetologica
pub_type: 杂志文章
doi:10.1007/s00592-016-0915-4
更新日期:2017-02-01 00:00:00
abstract:AIM:To identify the potential metabolite markers in diabetic retinopathy (DR) by using gas chromatography coupled with time-of-flight mass spectrometry (GC-TOFMS). METHODS:GC-TOFMS spectra were acquired from vitreous and aqueous humor (AH) samples of patients with DR and non-diabetic participants. Comparative analysis...
journal_title:Acta diabetologica
pub_type: 杂志文章
doi:10.1007/s00592-019-01363-0
更新日期:2020-01-01 00:00:00
abstract::Autonomic nervous dysfunction is considered to be one of the mechanisms of metabolic syndrome (MetS). The aim of this study is to investigate whether resting heart rate, a marker of autonomic nervous dysfunction, is a predictor of MetS in apparently healthy non-obese [body mass index (BMI) <25 kg/m(2)] Japanese men. T...
journal_title:Acta diabetologica
pub_type: 杂志文章
doi:10.1007/s00592-013-0503-9
更新日期:2014-02-01 00:00:00
abstract::The aim of this research was the evaluation of polymorphonuclear leukocyte (PMN) membrane fluidity in subjects with insulin resistance. Insulin sensitivity, in fact, may be influenced by plasma membrane fluidity. We enrolled 19 subjects with insulin resistance previously demonstrated during an euglycemic hyperinsuline...
journal_title:Acta diabetologica
pub_type: 杂志文章
doi:10.1007/s005920070029
更新日期:2000-03-01 00:00:00
abstract::The renin-angiotensin system (RAS) has an important role in the endocrine pancreas. Multiple researches have shown that even in the insulin resistance phase, there are many abnormalities in islets morphology and function. This study aimed at investigating the effects of RAS blockade on the islets function in rats with...
journal_title:Acta diabetologica
pub_type: 杂志文章
doi:10.1007/s00592-010-0210-8
更新日期:2013-08-01 00:00:00
abstract::Changes in the amounts and localization of protein kinase C (PKC) isoforms occur in galactosaemic lens epithelial cells. A link between PKC changes and myo-inositol depletion has been suggested. Raf-1, a component of a Ras pathway, is a substrate for PKC. Raf-1 levels were measured in galactosaemic lens epithelial cel...
journal_title:Acta diabetologica
pub_type: 杂志文章
doi:10.1007/s005920050119
更新日期:1998-10-01 00:00:00
abstract:AIMS:To assess the time-dependent effect of sleep quality on diabetes and coronary artery disease (CAD) incidence among young adults. METHODS:Incident rates of diabetes and CAD during a mean follow-up of 6.4 ± 4.1 years were assessed among 26,023 men (mean age 30.9 ± 5.6 years) of the Metabolic Lifestyle and Nutrition...
journal_title:Acta diabetologica
pub_type: 杂志文章
doi:10.1007/s00592-015-0779-z
更新日期:2016-04-01 00:00:00
abstract::Bovine insulin was glycated by in vitro incubation with 20-220 mM D-glucose for 1-48 h. The percentage of glycation was dependent on time, glucose concentration, temperature and pH, attaining values up to 28%. Glucose-lowering activities of glycated and control (non-glycated) insulin preparations were assessed in mice...
journal_title:Acta diabetologica
pub_type: 杂志文章
doi:10.1007/s005920050086
更新日期:1997-12-01 00:00:00
abstract::Diabetic neuropathy is a significant complication of chronic diabetes. We wished to discover whether we were successfully identifying patients who had developed neuropathic problems. Specifically, Diabetologists are traditionally poor at asking about erectile dysfunction (ED) and patients themselves are not always for...
journal_title:Acta diabetologica
pub_type: 杂志文章
doi:10.1007/s00592-008-0084-1
更新日期:2009-12-01 00:00:00
abstract:AIMS:This subanalysis of a cross-sectional, nationwide study was undertaken to assess the relationship between healthy lifestyle behaviors and multiple cardiovascular risk factors among people with type 2 diabetes mellitus (T2DM). METHODS:Data collected from 25,454 participants, including demographics, lifestyle behav...
journal_title:Acta diabetologica
pub_type: 杂志文章,多中心研究
doi:10.1007/s00592-017-0981-2
更新日期:2017-06-01 00:00:00
abstract:AIMS:We sought to investigate temporal trends in prediabetes prevalence among US adolescents using two definitions and evaluate relationships with obesity and a MetS-severity score. METHODS:We evaluated data from 5418 non-Hispanic white, non-Hispanic black, and Hispanic adolescents aged 12-19 participating in the Nati...
journal_title:Acta diabetologica
pub_type: 杂志文章
doi:10.1007/s00592-016-0958-6
更新日期:2017-04-01 00:00:00