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Polymorphonuclear leukocyte membrane fluidity before and after activation in subjects with insulin resistance.

Abstract：

:The aim of this research was the evaluation of polymorphonuclear leukocyte (PMN) membrane fluidity in subjects with insulin resistance. Insulin sensitivity, in fact, may be influenced by plasma membrane fluidity. We enrolled 19 subjects with insulin resistance previously demonstrated during an euglycemic hyperinsulinemic clamp. PMN membrane fluidity was studied by labeling intact cells with the fluorescent probe 1-[4-(trimethylamino)phenyl]-6-phenyl-1,3,5-hexatriene and calculating the fluorescence polarization degree. The measurement was made before and after incubation of PMNs with two activating agents: 4-phorbol 12-myristate 13-acetate (PMA) and N-formyl-methionyl-leucyl-phenylalanine (fMLP). The baseline data showed a reduction of PMN membrane fluidity in subjects with insulin resistance. After PMN activation with PMA and fMLP, no significant variation in membrane fluidity was present in PMNs from normals, while in those from subjects with insulin resistance a slight decrease in PMN membrane fluidity was found only after activation with fMLP. The behavior of PMN membrane fluidity, before and after activation, distinguishes insulin-resistant subjects from normal controls, although the effect cannot be directly correlated with the degree of insulin resistance.

journal_name

Acta Diabetol

journal_title

Acta diabetologica

authors

Caimi G,Sinagra D,Canino B,Scarpitta AM,Montana M,Bonaventura V,Lo Presti R

doi

10.1007/s005920070029

subject

Has Abstract

pub_date

2000-03-01 00:00:00

pages

9-12

issue

1

eissn

0940-5429

issn

1432-5233

journal_volume

37

pub_type

杂志文章

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