Abstract:
:Nematodes infections are responsible for debilitating conditions and economic losses in domestic animals as well as livestock and are considered an important public health problem due to the high prevalence in humans. The nematode resistance for drugs has been reported for livestock, highlighting the importance for development of new anthelmintic compounds. The aim of the current study was to apply and compare fluorimetric techniques using Sytox and propidium iodide for evaluating the viability of C. elegans larvae after treatment with anthelmintic drugs. These fluorescent markers were efficient to stain larvae treated with ivermectin and albendazole sulfoxide. We observed that densitometric values were proportional to the concentration of dead larvae stained with both markers. Furthermore, data on motility test presented an inverse correlation with fluorimetric data when ivermectin was used. Our results showed that lower concentrations of drugs were effective to interfere in the processes of cellular transport while higher drugs concentrations were necessary in order to result in any damage to cell integrity. The methodology described in this work might be useful for studies that aim to evaluate the viability of nematodes, particularly for testing of new anthelminthic compounds using an easy, economic, reproducible, and no time-consuming technique.
journal_name
Biomed Res Intjournal_title
BioMed research internationalauthors
Ferreira SR,Mendes TA,Bueno LL,de Araújo JV,Bartholomeu DC,Fujiwara RTdoi
10.1155/2015/879263subject
Has Abstractpub_date
2015-01-01 00:00:00pages
879263eissn
2314-6133issn
2314-6141journal_volume
2015pub_type
杂志文章abstract::Energy dispersive X-ray spectroscopy microanalysis (EDX), scanning electron microscopy (SEM), and Archimedes' Principle were used to determine the characteristics of inorganic filler particles in five dental alginates, including Cavex ColorChange (C), Hydrogum 5 (H5), Hydrogum (H), Orthoprint (O), and Jeltrate Plus (J...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/178064
更新日期:2014-01-01 00:00:00
abstract::We have developed a new "drug" and approach that appear to be effective in reducing arterial age. This "drug" represents a low, subtherapeutic dose of statin and sartan and particularly their low-dose combination. The improvement of arterial wall characteristics, also reflecting in a decrease of arterial age, was achi...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2015/235709
更新日期:2015-01-01 00:00:00
abstract::Alterations in pancreatic milieu to adapt to physiological shifts occurring in conditions of obesity and metabolic syndrome (MS) have been documented, though mechanisms leading to such a state have remained elusive so far. The data presented here tries to look at the gravity of metabolic insult during the early and pr...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/617569
更新日期:2013-01-01 00:00:00
abstract::Background. In most reports on ECMO treatment, advanced age is classified as a contraindication to VA ECMO. We attempted to investigate whether advanced age would be a main risk factor deciding VA ECMO application and performing VA ECMO support. We determined whether advanced age should be regarded as an absolute or r...
journal_title:BioMed research international
pub_type: 临床试验,杂志文章
doi:10.1155/2017/3505784
更新日期:2017-01-01 00:00:00
abstract::A potent fibrinolytic enzyme-producing Bacillus cereus IND1 was isolated from the Indian food, rice. Solid-state fermentation was carried out using agroresidues for the production of fibrinolytic enzyme. Among the substrates, wheat bran supported more enzyme production and has been used for the optimized enzyme produc...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/725064
更新日期:2014-01-01 00:00:00
abstract:Background:Adverse drug reactions (ADRs) are the known cause of morbidity and mortality, resulting in increased hospitalization, healthcare costs, complications, and decreased adherence in patients with depression. This study is aimed at evaluating adverse drug reactions due to antidepressants among patients with depre...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/6682928
更新日期:2020-11-16 00:00:00
abstract:Background:BMPER has been reported to be associated with the biological behavior of a few malignant tumors, but the mechanism is still unclear. We aimed to detect BMPER expression in ovarian epithelial tumor tissues and its effects on their biological behaviors, as well as to elucidate the possible mechanism. Methods:...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/3607436
更新日期:2020-03-24 00:00:00
abstract::Erythrocytes deformation is one of the exciting properties of erythrocytes. It is still under investigation by many of the researchers in different disciplines. The clinicians and researchers are still looking for a simple and efficient method to monitor and tracking the erythrocytes deformation. This research article...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2018/6067583
更新日期:2018-10-24 00:00:00
abstract::Duroia macrophylla popularly known as "cabeça-de-urubú," "apuruí," or "puruí-grande-da-mata" occurs in the Amazon Forest. Its leaves and branches were collected twice and extracted with dichloromethane and methanol. All extracts were subjected to phytochemical investigation and terpenes and flavonoids were found in al...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/605831
更新日期:2013-01-01 00:00:00
abstract::High mobility group box 1 (HMGB1) is an ubiquitous protein that plays different roles in the nucleus, cytoplasm, and extracellular space. It is an important DAMP molecule that allows communication between damaged or tumor cells and the immune system. Tumor cells exploit HMGB1's ability to activate intracellular pathwa...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/512027
更新日期:2015-01-01 00:00:00
abstract::T. reesei is an efficient cellulase producer and biomass degrader. To improve xylitol production in Trichoderma reesei strains by genetic engineering, two approaches were used in this study. First, the presumptive D-xylulokinase gene in T. reesei (xyiH), which has high homology to known fungi D-xylulokinase genes, was...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/169705
更新日期:2014-01-01 00:00:00
abstract::Tissues in the anterior segment of the eye are particular vulnerable to oxidative stress. To minimise oxidative stress, ocular tissues utilise a range of antioxidant defence systems which include nonenzymatic and enzymatic antioxidants in combination with repair and chaperone systems. However, as we age our antioxidan...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2013/207250
更新日期:2013-01-01 00:00:00
abstract::Despite remarkable therapeutic advances have been made in the last few decades, non-small cell lung cancer (NSCLC) is still one of the leading causes of death worldwide. Brain metastases are a common complication of a wide range of human malignancies and in particular NSCLC. Brain-derived neurotrophic factor (BDNF), b...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/4193541
更新日期:2020-11-17 00:00:00
abstract::The aims of the present research are to investigate the possible predictors of pancreatic cancer, in particular smoking status, alcohol consumption, hypercholesterolemia, and diabetes mellitus, in patients with histologically confirmed pancreatic carcinoma and to examine the synergism between risk factors. A case-cont...
journal_title:BioMed research international
pub_type: 临床试验,杂志文章
doi:10.1155/2014/481019
更新日期:2014-01-01 00:00:00
abstract::Picobirnavirus (PBV) which has been included in the list of viruses causing enteric infection in animals is highly versatile because of its broad host range and genetic diversity. PBVs are among the most recent and emerging small, nonenveloped viruses with a bisegmented double-stranded RNA genome, classified under a n...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2014/780752
更新日期:2014-01-01 00:00:00
abstract::Due to alarming increase in population and rapid industrialization, drinking water quality is being deteriorated day by day in Pakistan. This review sums up the outcomes of various research studies conducted for drinking water quality status of different areas of Pakistan by taking into account the physicochemical pro...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2017/7908183
更新日期:2017-01-01 00:00:00
abstract::Therapeutic approach for Alzheimer's disease (AD) is still deficient. To find active compounds from herbal medicine is of interest in the alleviation of AD symptoms. This study aimed to investigate the protective effects of Tanshinone IIA (TIIA) on memory performance and synaptic plasticity in a transgenic AD model at...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2016/7631801
更新日期:2016-01-01 00:00:00
abstract::To evaluate the role of Cystatin C (Cys-C) in tumorigenesis and progression of prostate cancer (PCa), we retrospectively collected the clinical information from the records of 492 benign prostatic hyperplasia (BPH), 48 prostatic intraepithelial neoplasia (PIN), and 173 PCa patients, whose disease was newly diagnosed a...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2017/7450459
更新日期:2017-01-01 00:00:00
abstract::Objectives. The aim of the study is to detect the prevalence and the characteristics of infraorbital canal and Haller's cells on panoramic radiography of edentulous patients. Methods. The study group comprised 291 panoramic radiographs of edentulous patients. Radiographs were interpreted for the visibility and charact...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2018/1293124
更新日期:2018-07-08 00:00:00
abstract:Objectives:To evaluate the role of short-term low-dose glucocorticoids in mild COVID-19 patients. Methods:We conducted a retrospective, cross-sectional, single-center study in Kunming, China. A total of 33 mild COVID-19 cases were divided into two treatment groups (with and without glucocorticoids, methylprednisolone,...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/2854186
更新日期:2020-09-23 00:00:00
abstract::Upon allergen challenge, DC subsets are recruited to target sites under the influence of chemotactic agents; however, details pertinent to their trafficking remain largely unknown. We investigated the kinetic profiles of blood and skin-infiltrating DC subsets in twelve atopic subjects receiving six weekly intradermal ...
journal_title:BioMed research international
pub_type: 临床试验,杂志文章
doi:10.1155/2014/231036
更新日期:2014-01-01 00:00:00
abstract::Among uterine structural abnormalities, myomas and adenomyosis represent two distinct, though frequently coexistent entities, with a remarkable prevalence in women of reproductive age. Various mechanisms have been proposed to explain the impact of each of them on reproductive outcome. In respect to myomas, current evi...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2017/5926470
更新日期:2017-01-01 00:00:00
abstract::It is unclear whether the methyltransferase-like 14 (METTL14) protein promotes or suppresses cancer growth. We examined the association between METTL14 expression, cancer progression, and patient prognosis in a total of 398 breast cancer tissue specimens. Significantly fewer cancer tissue specimens compared with norma...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/8823270
更新日期:2020-10-20 00:00:00
abstract::Despite the rapid progression of cancer pharmacotherapy, the high drug resistance of pancreatic ductal adenocarcinoma (PDA) makes it one of the most lethal malignancies. Therefore, there are high expectations associated with experimental therapies, such as electrochemotherapy (ECT). This technique involves the applica...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2018/7364539
更新日期:2018-03-19 00:00:00
abstract::As the heart transitions from one exercise intensity to another, changes in cardiac output occur, which are modulated by alterations in force development and calcium handling. Although the steady-state force-calcium relationship at various heart rates is well investigated, regulation of these processes during transiti...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/468548
更新日期:2015-01-01 00:00:00
abstract:Objective:To explore multiscale integrated analysis methods in identifying key regulators of esophageal cancer (ESCA). Methods:We downloaded the ESCA dataset from The Cancer Genome Atlas (TCGA) database, which contained RNA-seq data, miRNA-seq data, methylation data, and clinical phenotype information. Then, we combin...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/5603958
更新日期:2020-08-12 00:00:00
abstract::For the induction of robust humoral and cellular immune responses, a strong rationale exists to use vaccine-adjuvant combinations possessing both immune modulatory and enhanced delivery capabilities. Herein, we evaluated the combination of 2 different adjuvants, a TLR9 agonist, composed of synthetic oligodeoxynucleoti...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/636847
更新日期:2013-01-01 00:00:00
abstract::Surgical flaps are frequently affected by ischemia/reperfusion (I/R) injury. Calcium-sensing receptor (CaSR) and stromal cell-derived factor-1α (SDF-1α) are closely associated with myocardial I/R injury. This study was performed to evaluate the feasibility of applying SDF-1α to counteract CaSR activation-mediated I/R ...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2018/8945850
更新日期:2018-01-11 00:00:00
abstract::Nitric oxide (NO) has a wide variety of physiological functions in the kidney. Besides the regulatory effects in intrarenal haemodynamics and glomerular microcirculation, in vivo studies reported the diuretic and natriuretic effects of NO. However, opposite results showing the stimulatory effect of NO on Na+ reabsorpt...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2017/6871081
更新日期:2017-01-01 00:00:00
abstract:OBJECTIVE:The purpose of this study was to assess the value of ecological momentary assessment in evaluating physical activity among children, adolescents, and adults. It also determines whether ecological momentary assessment fulfills the criteria of validity, reliability, objectivity, norms, and standardization appli...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2014/915172
更新日期:2014-01-01 00:00:00