Abstract:
:A potent fibrinolytic enzyme-producing Bacillus cereus IND1 was isolated from the Indian food, rice. Solid-state fermentation was carried out using agroresidues for the production of fibrinolytic enzyme. Among the substrates, wheat bran supported more enzyme production and has been used for the optimized enzyme production by statistical approach. Two-level full-factorial design demonstrated that moisture, supplementation of beef extract, and sodium dihydrogen phosphate have significantly influenced enzyme production (P < 0.05). A central composite design resulted in the production of 3699 U/mL of enzyme in the presence of 0.3% (w/w) beef extract and 0.05% (w/w) sodium dihydrogen phosphate, at 100% (v/w) moisture after 72 h of fermentation. The enzyme production increased fourfold compared to the original medium. This enzyme was purified to homogeneity by ammonium sulfate precipitation, diethylaminoethyl-cellulose ion-exchange chromatography, Sephadex G-75 gel filtration chromatography, and casein-agarose affinity chromatography and had an apparent molecular mass of 29.5 kDa. The optimum pH and temperature for the activity of fibrinolytic enzyme were found to be 8.0 and 60°C, respectively. This enzyme was highly stable at wide pH range (7.0-9.0) and showed 27% ± 6% enzyme activity after initial denaturation at 60°C for 1 h. In vitro assays revealed that the enzyme could activate plasminogen and significantly degraded the fibrin net of blood clot, which suggests its potential as an effective thrombolytic agent.
journal_name
Biomed Res Intjournal_title
BioMed research internationalauthors
Vijayaraghavan P,Vincent SGdoi
10.1155/2014/725064subject
Has Abstractpub_date
2014-01-01 00:00:00pages
725064eissn
2314-6133issn
2314-6141journal_volume
2014pub_type
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/849080
更新日期:2014-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
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journal_title:BioMed research international
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journal_title:BioMed research international
pub_type: 杂志文章,评审
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journal_title:BioMed research international
pub_type: 临床试验,杂志文章
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journal_title:BioMed research international
pub_type: 杂志文章,评审
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pub_type: 杂志文章,评审
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更新日期:2014-01-01 00:00:00
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更新日期:2019-04-04 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/413708
更新日期:2014-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1155/2017/7243909
更新日期:2017-01-01 00:00:00
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journal_title:BioMed research international
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doi:10.1155/2014/871576
更新日期:2014-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/348659
更新日期:2014-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/169705
更新日期:2014-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2019/2019846
更新日期:2019-06-19 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2016/7529521
更新日期:2016-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章,评审
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/7694734
更新日期:2020-09-16 00:00:00
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journal_title:BioMed research international
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doi:10.1155/2014/261761
更新日期:2014-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
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更新日期:2017-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/129629
更新日期:2013-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/948473
更新日期:2015-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2017/2543262
更新日期:2017-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/618021
更新日期:2015-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2019/3126376
更新日期:2019-08-19 00:00:00
abstract:Objective:Epidemiological studies have shown a correlation between periodontal disease (PD) and age-related macular degeneration (AMD). However, the results have been inconsistent, and no relevant meta-analysis has been performed on this topic. Hence, we performed a meta-analysis to evaluate whether the two diseases ar...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2020/4753645
更新日期:2020-09-28 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/858907
更新日期:2015-01-01 00:00:00