Abstract:
:Despite the rapid progression of cancer pharmacotherapy, the high drug resistance of pancreatic ductal adenocarcinoma (PDA) makes it one of the most lethal malignancies. Therefore, there are high expectations associated with experimental therapies, such as electrochemotherapy (ECT). This technique involves the application of short electric pulses to induce transitional permeabilization of the cellular membrane, thus enhancing drug molecules influx. The aim of the study was to investigate the influence of electroporation with cisplatin (CisEP) on the primary culture of human PDA cells from lung metastases-their survival and stress response. Considering the growing importance of various research models, two established human PDA cell lines, EPP85-181P (sensitive to daunorubicin) and EPP85-181RDB (resistant to daunorubicin), were utilized as a reference control. Cisplatin revealed higher cytotoxicity towards established cell lines. Following CisEP application, we observed a significant decrease of cells viability in the primary culture model. After CisEP therapy, an increased immunoreactivity with SOD-2 and Casp-3 antibodies was noticed. In conclusion, we discovered that electroporation can enhance the cytotoxic effect of cisplatin in pancreatic cancer cells in vitro. This effect was evident for cells from the primary culture. The obtained results confirm the importance of primary cells models in studies on the efficacy of experimental cancer therapies.
journal_name
Biomed Res Intjournal_title
BioMed research internationalauthors
Michel O,Kulbacka J,Saczko J,Mączyńska J,Błasiak P,Rossowska J,Rzechonek Adoi
10.1155/2018/7364539subject
Has Abstractpub_date
2018-03-19 00:00:00pages
7364539eissn
2314-6133issn
2314-6141journal_volume
2018pub_type
杂志文章abstract::This study aimed to evaluate the genes that were expressed in the healing bones around SLA-treated titanium orthodontic mini-implants in a beagle at early (1-week) and late (4-week) stages with RNA-sequencing (RNA-Seq). Samples from sites of surgical defects were used as controls. Total RNA was extracted from the tiss...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/538080
更新日期:2015-01-01 00:00:00
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journal_title:BioMed research international
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journal_title:BioMed research international
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journal_title:BioMed research international
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更新日期:2018-04-23 00:00:00
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doi:10.1155/2018/6067583
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pub_type: 杂志文章,随机对照试验
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更新日期:2014-01-01 00:00:00
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journal_title:BioMed research international
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doi:10.1155/2019/2019846
更新日期:2019-06-19 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2013/547902
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pub_type: 杂志文章,评审
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journal_title:BioMed research international
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journal_title:BioMed research international
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更新日期:2013-01-01 00:00:00
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journal_title:BioMed research international
pub_type: 杂志文章
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更新日期:2019-08-20 00:00:00
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journal_title:BioMed research international
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pub_type: 杂志文章
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更新日期:2020-04-19 00:00:00