Abstract:
:CuO nanoparticles (CuO-NPs) serve several important functions in human life, particularly in the fields of medicine, engineering, and technology. These nanoparticles have been utilized as catalysts, semiconductors, sensors, gaseous and solid ceramic pigments, and magnet rotatable devices. Further use for CuO-NPs has been employed in the pharmaceutical industry especially in the production of anti-microbial fabric treatments or prevention of infections caused by Escherichia coli and methicillin-resistant Staphylococcus aureus. Two key potential routes of exposure to CuO-NPs exist through inhalation and skin exposure. Toxicity of these nanoparticles has been reported in various studies; however, no study as of yet has investigated the complete cellular mechanisms involved in CuO-NPs toxicity on human cells. The aim of this study was to determine the cytotoxicity of CuO-NPs on human blood lymphocytes. Blood lymphocytes were obtained from healthy male subjects through the use of Ficoll polysaccharide subsequently by gradient centrifugation. The following parameters were assayed in blood lymphocytes after a 6-h incubation with different concentrations of CuO-NPs: cell viability, reactive oxygen species (ROS) formation, lipid peroxidation, cellular glutathione levels, and mitochondrial and lysosomal damage. Our results demonstrate that CuO-NPs, in particular, decreased cell viability in a concentration-dependent manner and the IC50 determined was 382 μM. CuO-NP cytotoxicity was associated with significant increase at intracellular ROS level and loss of mitochondrial membrane potential and lysosomal membrane leakiness. Hence, CuO-NPs are shown to effectively induce oxidative stress in addition to inflict damage on mitochondria and lysosomes in human blood lymphocytes.
journal_name
Biol Trace Elem Resjournal_title
Biological trace element researchauthors
Assadian E,Zarei MH,Gilani AG,Farshin M,Degampanah H,Pourahmad Jdoi
10.1007/s12011-017-1170-4subject
Has Abstractpub_date
2018-08-01 00:00:00pages
350-357issue
2eissn
0163-4984issn
1559-0720pii
10.1007/s12011-017-1170-4journal_volume
184pub_type
杂志文章abstract::Comparison of the clastogenic effects of antimony and bismuth used as trioxides, when administered orally by gavaging to laboratory bred male mice, showed that the former was more strongly clastogenic than the latter. Three doses of each chemical (400, 666.67, and 1000 mg/kg body wt), corresponding to 1/50, 1/30, and ...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/BF02783802
更新日期:1993-05-01 00:00:00
abstract::The aim of this study was to investigate the relationships between the bronchopneumonia and mean concentrations of those trace elements in bronchoalveolar lavage fluid (BALF). Twenty-nine dogs were included this study (17 healthy dogs and 12 dogs with respiratory disease). Each BALF sample had been obtained during bro...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-008-8149-0
更新日期:2008-07-01 00:00:00
abstract::Instrumental neutron activation (INAA) with k0 standardization has been used to determine the concentration levels of a variety of major and trace elements (Al, As, Br, Cd, Cl, Co, Cr, Cs, Cu, Fe, Hg, K, La, Mg, Mn, Mo, Na, Rb, Sc, Se, and Zn) in rice grains (raw and parboiled) and in rice flour collected from local m...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1385/BTER:103:1:083
更新日期:2005-01-01 00:00:00
abstract::Selenium is an important nutrient for humans and livestock. Soil selenium concentration in the world is highly variable; deficiency and toxicity occur in populations living short distance apart. Knowledge of Se concentrations in humans and the environments, especially because the range for toxicity and deficiency is n...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-018-1489-5
更新日期:2019-06-01 00:00:00
abstract::Activity of the immunoregulatory peptide thymulin reflects differences in zinc status. This study compared thymulin activity with four other zinc status measures in rats fed zinc at either 5 or 25 ppm. Rats fed the lower zinc showed the following results compared with rats with adequate zinc intake: serum thymulin act...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-020-02159-y
更新日期:2021-02-01 00:00:00
abstract::This study assessed the effect of dietary selenium (Se) deficiency on male reproductive function in chicken. A total of 180 Hy-line laying cocks (1 day old; Weiwei) were randomly divided into 2 groups (n = 90) of Se-deficient chickens and control chickens. The control group was fed a basic diet (containing 0.15 mg of ...
journal_title:Biological trace element research
pub_type: 杂志文章,随机对照试验
doi:10.1007/s12011-015-0534-x
更新日期:2016-06-01 00:00:00
abstract::In this study, a solid-phase extraction method combined with atomic absorption spectrometry for extraction, preconcentration, and determination of iron (Fe(3+)), copper (Cu(2+)), and lead (Pb(2+)) ions at trace levels in water samples has been reported. The influences of effective parameters such as flow rate, pH, elu...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-011-9171-1
更新日期:2012-02-01 00:00:00
abstract::Sediments from 14 stations in the Foshan Waterway, a river crossing the industrial district of Guangdong Province, South China, were sampled and subsequently analyzed. The 14 stations were selected for the pollution discharging features of the river, such as the hydrology, the distribution of pollution sources, and th...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-013-9789-2
更新日期:2013-11-01 00:00:00
abstract::The aim of this work was to explore the effects of a low- and high-zinc diet and vitamin Aon the distribution of fatty acids in chylomicrons. Mongolian Gerbils were fed a basal diet (for 3 wk) containing 8 or 38 mg zinc/kg of feed (low-zinc group [termed LZ group] and saturated zinc group [termed SZ group], respective...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1385/BTER:112:2:145
更新日期:2006-08-01 00:00:00
abstract::The objective was to determine if a mammary cell line shows glucocorticoid stimulation of Zn uptake, and to determine whether polyamines mediate this stimulation. 65Zn uptake by COMMA-1D mouse mammary epithelial cells over a 24-h period increased significantly in cells administered 10(-7) or 10(-6) M hydrocortisone. I...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/BF02783193
更新日期:1993-11-01 00:00:00
abstract::The effects of selenium were investigated on three human colon cancer cell lines: Caco 2, HRT 18, and HT 29. At low concentrations (10-100 nM), selenium stimulated cell growth in serum-free medium. Thus, selenium is an essential trace element for cell proliferation. At higher concentrations, selenium inhibited cell gr...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/BF02919096
更新日期:1989-04-01 00:00:00
abstract::This study evaluated dental fluorosis of the incisors and immunoreactivity in the brain tissues of rats given chronic fluoride doses pre- and postnatally. Female rats were given drinking water with 0, 30 or 100 ppm fluoride ad libitum throughout gestation and the nursing period. In addition, 63 male offspring were tre...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-016-0695-2
更新日期:2016-11-01 00:00:00
abstract::Urine is a clinical specimen often used in medical diagnostics for monitoring of elements concentrations and kidneys function. We determined the contents of magnesium (Mg), calcium (Ca), zinc (Zn), copper (Cu), iron (Fe), lead (Pb), and cadmium (Cd) in 74 samples of 24-h urine (from 46 women and 28 men). The measureme...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-010-8745-7
更新日期:2011-07-01 00:00:00
abstract::Women with preeclampsia have been shown to have elevated blood levels of the metabolite homocysteine, and alterations in blood levels of zinc and copper have also been reported. This study measured plasma levels of zinc, copper, and homocysteine in women with preeclampsia and in women with healthy, normotensive pregna...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1385/BTER:104:2:097
更新日期:2005-05-01 00:00:00
abstract::Systemic lupus erythematosus (SLE) is a chronic inflammatory autoimmune disease of multifactorial origin. Studies have shown that trace elements such as zinc and copper may help maintain optimum function of the immune system and metabolism, while toxic metals such as lead may increase systemic autoimmunity. The curren...
journal_title:Biological trace element research
pub_type: 临床试验,杂志文章
doi:10.1007/s12011-018-1592-7
更新日期:2019-09-01 00:00:00
abstract::Trace element involvement in wounds left to heal by secondary intention needs clarification. We have previously reported faster healing of wounds following acute surgery compared with elective excision of pilonidal sinus disease. The effect of topical zinc on the closure of the excisional wounds was mediocre compared ...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-013-9658-z
更新日期:2013-06-01 00:00:00
abstract::Calcium, magnesium, and zinc balances were determined in eleven young adult college females (mean age, 24.9±2.35) during a 39-d metabolic study when the subjects were fed an adequate calorie and protein diet based on habitually consumed foods. Analysis showed that the dietary contribution of calcium, magnesium, and zi...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/BF02917454
更新日期:1985-11-01 00:00:00
abstract::Multielemental detection using Instrumental Neutron Activation Analysis (INAA) and Proton-Induced X-Ray Emission (PIXE) of drinkable water of Florence (Italy) is described. The concentrations of 52 elements were measured. No chemical treatment was performed on the samples, before or after irradiation. On the basis of ...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/BF02992691
更新日期:1990-07-01 00:00:00
abstract::Yerba mate (Ilex paraguariensis), a widely consumed beverage in South America, contains various biochemically active substances, among them are several minerals. This paper reports on the results of a survey of trace elements in the yerba mate infusions. Three different commercially available trademarks of I. paraguar...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-011-9056-3
更新日期:2011-12-01 00:00:00
abstract::Elemental concentrations in head hair and blood (whole-blood, erythrocytes and plasma) of 28 Nigerian subjects were determined using instrumental neutron activation analysis (INAA) and proton-induced X-ray emission (PIXE) techniques. Correlations found between different elements in hair and blood are reported. Hair le...
journal_title:Biological trace element research
pub_type: 临床试验,杂志文章
doi:10.1007/BF02917347
更新日期:1994-10-01 00:00:00
abstract::Lipid peroxy radicals (ROO.) were detected by electron spin resonance (ESR) at low temperature after formation by addition of H2O2 into a suspension of mice lymphocytes. If lymphocytes were treated with selenomethionine (Se-Met) prior to addition of H2O2, ROO. formation was inhibited in a fashion that was dependent on...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/BF02783233
更新日期:1997-01-01 00:00:00
abstract::Abnormal placentation is the likely cause of the slow fetal growth and the high levels of circulating lipid peroxides found in severe pre-eclampsia. These peroxides are probably responsible for the high thromboxane:prostacyclin ratio found in this disease and may participate in the endothelial cell damage which is its...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/BF02784164
更新日期:1996-10-01 00:00:00
abstract::To obtain real-time dynamic changes of non-protein-bound copper in the blood, we have developed an online microdialysis sampling system coupled with a flow-injection graphite furnace-atomic absorption spectrometer (FI-GFAAS). The analytical performances of the online system such as linearity, limit of detection, preci...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1385/BTER:111:1:255
更新日期:2006-07-01 00:00:00
abstract::The effect of aging on the status of macrominerals and trace elements in tissues was studied using two strains (SAMP1 and SAMR1) of senescence accelerated mouse. Two-month-old, 6-mo-old, and 10-mo-old female SAMP1 and SAMR1 mice were fed a commercial diet. Iron, zinc, copper, calcium, magnesium, phosphorus, sulfur, so...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1385/BTER:80:1:53
更新日期:2001-04-01 00:00:00
abstract::The binding of Cu(II), Zn(II), Ni(II), and Cd(II) to protein components in serum, placenta, kidney, and urine was investigated at physiological pH, using radioisotopes as tracers. All the four metals were bound to albumin and other macromolecules in serum. However, small amounts were also bound to low molecular weight...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/BF02917246
更新日期:1989-07-01 00:00:00
abstract::Despite the name, rare earth elements are relatively abundant in soil. Therefore, these elements might interact with biosphere during the history of life. In this study, we have examined the effect of rare earth ions on the growth of bacteria, fungi and soil nematode. All rare earth ions, except radioactive promethium...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-016-0727-y
更新日期:2016-12-01 00:00:00
abstract::The toxic effect of Pb2+ has been studied in eukaryotic cells by using Tetrahymena as a target. The maximum power (P(m)) and the growth rate constant (k) were determined, which showed that values of P(m) and k were linked to the concentration (C) of Pb2+. The addition of Pb2+ caused a decrease of the maximum heat prod...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-007-0055-3
更新日期:2007-11-01 00:00:00
abstract::No specific treatment for COVID-19 infection is available up till now, and there is a great urge for effective treatment to reduce morbidity and mortality during this pandemic. We aimed to evaluate the effect of combining chloroquine/hydroxychloroquine (CQ/HCQ) and zinc in the treatment of COVID-19 patients. This was ...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-020-02512-1
更新日期:2020-11-27 00:00:00
abstract::To elucidate compositional changes of the mitral valve with aging, the authors investigated the relationships among element contents in the mitral valves. The subjects consisted of 10 men and 15 women, ranging in age from 65 to 102 yr. After the ordinary dissection, mitral valves, lower parts of the interventricular s...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1385/BTER:88:3:203
更新日期:2002-09-01 00:00:00
abstract::Quercetin, a plant-derived flavonoid, is frequently used as an antioxidant for efficient anti-oxidative capacity. However, whether quercetin has protective effects on goat sperm and preimplantation embryos against Cd2+-induced oxidative injury is still unclear. So, we researched the influence of quercetin on goat sper...
journal_title:Biological trace element research
pub_type: 杂志文章
doi:10.1007/s12011-018-1255-8
更新日期:2018-10-01 00:00:00