Abstract：
:T cells from systemic lupus erythematosus (SLE) patients display a wide array of anomalies in peripheral immune tolerance mechanisms. The role of ubiquitin ligases such as Cbl-b has been described recently in these phenomena. However, its role in resistance to suppression phenotype in SLE has not been characterized, which was the aim of the present study. Thirty SLE patients (20 with active disease and 10 with complete remission) and 30 age- and sex-matched healthy controls were recruited. Effector (CD4+ CD25- ) and regulatory (CD4+ CD25+ ) T cells (Tregs ) were purified from peripheral blood mononuclear cells (PBMCs) by magnetic selection. Suppression assays were performed in autologous and allogeneic co-cultures and analysed by a flow cytometry assay. Cbl-b expression and lysine-63 (K63)-specific polyubiquitination profile were assessed by Western blotting. We found a defective Cbl-b expression in Tregs from lupus patients in contrast to healthy controls (1·1 ± 0·9 versus 2·5 ± 1·8, P = 0·003), which was related with resistance to suppression (r = 0·633, P = 0·039). Moreover, this feature was associated with deficient K63 polyubiquitination substrates and enhanced expression of phosphorylated signal transducer and activation of transcription 3 (pSTAT-3) in Tregs from lupus patients. Our findings support that Cbl-b modulates resistance to suppression by regulating the K63 polyubiquitination profile in lupus Tregs . In addition, defective K63 polyubiquitination of STAT-3 is related to increased pSTAT-3 expression, and might promote the loss of suppressive capacity of Tregs in lupus patients.
journal_name
Clin Exp Immunoljournal_title
Clinical and experimental immunologyauthors
Romo-Tena J,Rajme-López S,Aparicio-Vera L,Alcocer-Varela J,Gómez-Martín Ddoi
10.1111/cei.13054subject
Has Abstractpub_date
2018-01-01 00:00:00pages
42-49issue
1eissn
0009-9104issn
1365-2249journal_volume
191pub_type
杂志文章abstract：:Members of the European Society for Immunodeficiencies (ESID) and other colleagues have updated the multi-stage expert-opinion-based diagnostic protocol for non-immunologists incorporating newly defined primary immunodeficiency diseases (PIDs). The protocol presented here aims to increase the awareness of PIDs among d...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章,评审
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abstract：:Recent studies have suggested that a high percentage of Epstein-Barr virus (EBV)-infected lymphocytes in peripheral blood of patients with chronic, active EBV infection (CAEBV) is of T cell origin. Although T cells are expanded oligoclonally in CAEBV, it is not clear whether the restricted diversity of T cells arise f...
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pub_type： 杂志文章
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journal_title：Clinical and experimental immunology
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doi：
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
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doi：10.1046/j.1365-2249.1996.d01-623.x
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
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pub_type： 杂志文章
doi：10.1111/j.1365-2249.1990.tb05287.x
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1995.tb05573.x
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1980-03-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1995.tb03658.x
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/cei.12366
更新日期：2014-09-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1979-11-01 00:00:00
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pub_type： 杂志文章
doi：10.1111/j.1365-2249.1990.tb03331.x
更新日期：1990-08-01 00:00:00
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pub_type： 杂志文章
doi：10.1111/j.1365-2249.1990.tb03298.x
更新日期：1990-06-01 00:00:00
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pub_type： 杂志文章
doi：
更新日期：1976-01-01 00:00:00
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更新日期：2006-03-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
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更新日期：1997-09-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1975-02-01 00:00:00
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pub_type： 杂志文章
doi：10.1046/j.1365-2249.2002.01856.x
更新日期：2002-06-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1982-10-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1989-12-01 00:00:00
abstract：:The depressed cell-mediated immunity in rheumatoid arthritis was investigated in vivo by cutaneous hypersensitivity responses to seven antigens including tuberculin PPD, and in vitro by lymphocyte transformation to the latter antigen. In vivo 40% of rheumatoid patients were anergic compared to 2% of controls (P less t...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1984-07-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1977-09-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1981-11-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1986-02-01 00:00:00
