Abstract：
:First insights on the human gut microbiome have been gained from medium-sized, cross-sectional studies. However, given the modest portion of explained variance of currently identified covariates and the small effect size of gut microbiota modulation strategies, upscaling seems essential for further discovery and characterisation of the multiple influencing factors and their relative contribution. In order to guide future research projects and standardisation efforts, we here review currently applied collection and preservation methods for gut microbiome research. We discuss aspects such as sample quality, applicable omics techniques, user experience and time and cost efficiency. In addition, we evaluate the protocols of a large-scale microbiome cohort initiative, the Flemish Gut Flora Project, to give an idea of perspectives, and pitfalls of large-scale faecal sampling studies. Although cryopreservation can be regarded as the gold standard, freezing protocols generally require more resources due to cold chain management. However, here we show that much can be gained from an optimised transport chain and sample aliquoting before freezing. Other protocols can be useful as long as they preserve the microbial signature of a sample such that relevant conclusions can be drawn regarding the research question, and the obtained data are stable and reproducible over time.
journal_name
FEMS Microbiol Revjournal_title
FEMS microbiology reviewsauthors
Vandeputte D,Tito RY,Vanleeuwen R,Falony G,Raes Jdoi
10.1093/femsre/fux027subject
Has Abstractpub_date
2017-08-01 00:00:00pages
S154-S167issue
Supp_1eissn
0168-6445issn
1574-6976pii
3902998journal_volume
41pub_type
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更新日期：2017-03-01 00:00:00
