Simultaneous measurements of photocurrents and H2O2 evolution from solvent exposed photosystem 2 complexes.

Abstract:

:In plants, algae, and cyanobacteria, photosystem 2 (PS2) catalyzes the light driven oxidation of water. The main products of this reaction are protons and molecular oxygen. In vitro, however, it was demonstrated that reactive oxygen species like hydrogen peroxide are obtained as partially reduced side products. The transition from oxygen to hydrogen peroxide evolution might be induced by light triggered degradation of PS2's active center. Herein, the authors propose an analytical approach to investigate light induced bioelectrocatalytic processes such as PS2 catalyzed water splitting. By combining chronoamperometry and fluorescence microscopy, the authors can simultaneously monitor the photocurrent and the hydrogen peroxide evolution of light activated, solvent exposed PS2 complexes, which have been immobilized on a functionalized gold electrode. The authors show that under limited electron mediation PS2 displays a lower photostability that correlates with an enhanced H2O2 generation as a side product of the light induced water oxidation.

journal_name

Biointerphases

journal_title

Biointerphases

authors

Vöpel T,Ning Saw E,Hartmann V,Williams R,Müller F,Schuhmann W,Plumeré N,Nowaczyk M,Ebbinghaus S,Rögner M

doi

10.1116/1.4938090

subject

Has Abstract

pub_date

2015-03-22 00:00:00

pages

019001

issue

1

eissn

1934-8630

issn

1559-4106

journal_volume

11

pub_type

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