Abstract:
:Reliable, direct "sample-to-answer" capture of nucleic acid targets from complex media would greatly improve existing capabilities of DNA microarrays and biosensors. This goal has proven elusive for many current nucleic acid detection technologies attempting to produce assay results directly from complex real-world samples, including food, tissue, and environmental materials. In this study, we have investigated mixed self-assembled thiolated single-strand DNA (ssDNA) monolayers containing a short thiolated oligo(ethylene glycol) (OEG) surface diluent on gold surfaces to improve the specific capture of DNA targets from complex media. Both surface composition and orientation of these mixed DNA monolayers were characterized with x-ray photoelectron spectroscopy (XPS) and near-edge x-ray absorption fine structure (NEXAFS). XPS results from sequentially adsorbed ssDNA/OEG monolayers on gold indicate that thiolated OEG diluent molecules first incorporate into the thiolated ssDNA monolayer and, upon longer OEG exposures, competitively displace adsorbed ssDNA molecules from the gold surface. NEXAFS polarization dependence results (followed by monitoring the N 1s-->pi(*) transition) indicate that adsorbed thiolated ssDNA nucleotide base-ring structures in the mixed ssDNA monolayers are oriented more parallel to the gold surface compared to DNA bases in pure ssDNA monolayers. This supports ssDNA oligomer reorientation towards a more upright position upon OEG mixed adlayer incorporation. DNA target hybridization on mixed ssDNA probe/OEG monolayers was monitored by surface plasmon resonance (SPR). Improvements in specific target capture for these ssDNA probe surfaces due to incorporation of the OEG diluent were demonstrated using two model biosensing assays, DNA target capture from complete bovine serum and from salmon genomic DNA mixtures. SPR results demonstrate that OEG incorporation into the ssDNA adlayer improves surface resistance to both nonspecific DNA and protein adsorption, facilitating detection of small DNA target sequences from these undiluted, unpurified complex biological mixtures unachievable with previously reported, analogous ssDNA/11-mercapto-1-undecanol monolayer surfaces [P. Gong, C.-Y. Lee, L. J. Gamble, D. G. Castner, and D. W. Grainger, Anal. Chem. 78, 3326 (2006)].
journal_name
Biointerphasesjournal_title
Biointerphasesauthors
Lee CY,Gamble LJ,Grainger DW,Castner DGdoi
10.1116/1.2219110subject
Has Abstractpub_date
2006-06-01 00:00:00pages
82-92issue
2eissn
1934-8630issn
1559-4106journal_volume
1pub_type
杂志文章相关文献
Biointerphases文献大全abstract::Neuronal process growth is guided by extrinsic environmental cues such as extracellular matrix (ECM) proteins. Recent reports have described that the growth cone extension is superior across gradients of the ECM protein laminin compared to growth across uniformly distributed laminin. In this work, the authors have pre...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4857295
更新日期:2014-03-01 00:00:00
abstract::Peptide nucleic acids (PNAs) are mimics of oligonucleotides containing a neutral peptidelike backbone and are able to bind complementary DNA targets with high affinity and selectivity. In order to investigate the effect of the ionic strength of the buffer solution, hybridization experiments with PNAs as (catcher) prob...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.2746871
更新日期:2007-06-01 00:00:00
abstract::A drug-eluting coating applied onto biomedical devices and implants is an appropriate way to ensure that an inhibitory concentration of antimicrobial drugs is present at the device surface, thus preventing surface colonization and subsequent biofilm formation. In this study, a thin polymer coating was applied to mater...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/6.0000511
更新日期:2020-12-18 00:00:00
abstract::Permanent implants made from titanium are widely used and successfully implemented in medicine to address problems related to orthopedic and oral disorders. However, implants that interact in all cases optimally and durably with bone tissue have yet to be developed. Here, the authors suggest a phospholipids 1-palmitoy...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4938556
更新日期:2015-03-29 00:00:00
abstract::Bacterial colonies that reside on a surface, known as biofilms, are intrinsically impenetrable to traditional antibiotics, ultimately driving research toward an alternative therapeutic approach. Nitric oxide (NO) has gained attention for its biologically beneficial properties, particularly centered around its antibact...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4962266
更新日期:2016-09-07 00:00:00
abstract::The articular cartilage, once injured, has a limited capacity for intrinsic repair. Preparation of functionally biocartilage substitutes in vitro for cartilage repair is an attractive concept with the recent advances in tissue engineering. In this study, adipose-derived adult stem cells (ADAS) and chondrocytes (Ch) we...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4942754
更新日期:2016-03-25 00:00:00
abstract::Shewanella oneidensis MR-1 is a dissimilatory metal-reducing bacterium capable of reducing various metal and sulfur compounds and precipitating them in nanoparticulate form. Here, we report the synthesis of molybdenum disulfide nanomaterials at the site of S. oneidensis biofilms grown in the presence of molybdenum tri...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/6.0000199
更新日期:2020-07-24 00:00:00
abstract::Friction force microscopy was employed for the tribological investigation of human head hair in two different environments: a dry atmosphere and de-ionized water. The fibers were immobilized by embedding them in indium. The effects of bleaching, conditioning, and immersion in methanolic KOH were quantified in terms of...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.3432462
更新日期:2010-06-01 00:00:00
abstract::Cationic amphiphilic peptides have been engineered to target both Gram-positive and Gram-negative bacteria while avoiding damage to other cell types. However, the exact mechanism of how these peptides target, bind, and disrupt bacterial cell membranes is not understood. One specific peptide that has been engineered to...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4982710
更新日期:2017-05-05 00:00:00
abstract::This contribution reports on grafting of bioactive polymers such as poly(sodium styrene sulfonate) (polyNaSS) onto titanium (Ti) surfaces. This grafting process uses a modified dopamine as an anchor molecule to link polyNaSS to the Ti surface. The grafting process combines reversible addition-fragmentation chain trans...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4985608
更新日期:2017-06-14 00:00:00
abstract::The growing interest in artificial bioorganic interfaces as a platform for applications in emerging areas as personalized medicine, clinical diagnostics, biosensing, biofilms, prevention of biofouling, and other fields of bioengineering is the origin of a need for in detail multitechnique characterizations of such lay...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4948341
更新日期:2016-06-02 00:00:00
abstract::In this systematic review, the authors explored the surface aspects of various titanium (Ti) or Ti alloy medical implants, examining the interface formed between the implant and surrounding nonkeratinized soft tissues (periosteum, muscles, tendons, fat, cicatrix, or dura mater). A comprehensive search undertaken in Ju...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.5113607
更新日期:2019-08-16 00:00:00
abstract::Dentin adhesive systems for composite tooth restorations are composed of hydrophilic/hydrophobic monomers, solvents, and photoinitiators. The adhesives undergo phase separation and concomitant compositional change during their application in the wet oral environment; phase separation compromises the quality of the hyb...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.5058072
更新日期:2018-12-17 00:00:00
abstract::Supported phospholipid bilayers are interesting model systems for biologists and present fascinating physical properties. The authors present an extensive experimental study of the dynamic properties of supported bilayers. The structure and the equilibrium properties of single and double supported bilayers were invest...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.2936938
更新日期:2008-06-01 00:00:00
abstract::The aim was to compare the protein release from normal human osteoblasts (NHO) cultured on scaffolds with similar morphology but different coatings. Different ceramic coatings; TiO(2), SiO(2) and calcium phosphate (CaP); Ca(9)HPO(4)(PO(4))(5)OH, were applied to porous TiO(2) scaffolds prepared by polymer sponge replic...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1007/s13758-012-0036-8
更新日期:2012-12-01 00:00:00
abstract::Gold nanoparticles (AuNPs) functionalized with a short chain amine-terminated alkanethiol (HS-(CH(2))(2)NH(2) or C2 NH(2)-thiol) are prepared via a direct synthesis method and then ligand-exchanged with a long chain amine-terminated alkanethiol (HS-(CH(2))(11)NH(2) or C11 NH(2)-thiol). Transmission electron microscopy...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.3622481
更新日期:2011-09-01 00:00:00
abstract::There has been much recent interest in the protein "corona," the nonspecific adsorption of proteins on the surface of nanoparticles used in biological applications. This research investigates an analogous DNA corona. We find that particles (200 nm and 1 μm) incubated with DNA form a DNA corona, with a higher concentra...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/6.0000439
更新日期:2020-10-01 00:00:00
abstract::The modeling, fabrication, cell loading, and mechanical and in vitro biological testing of biomimetic, interlockable, laser-made, concentric 3D scaffolds are presented. The scaffolds are made by multiphoton polymerization of an organic-inorganic zirconium silicate. Their mechanical properties are theoretically modeled...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4922646
更新日期:2015-06-23 00:00:00
abstract::Although it is well known that the textural properties of scaffolds play an important role in the process of tissue regeneration, the investigation of such effects remain difficult especially at the micro/nano level. Texture confers the material the additional ability to entrap/concentrate molecules circulating in the...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1007/s13758-012-0037-7
更新日期:2012-12-01 00:00:00
abstract::Antimicrobial peptides (AMPs) are attractive as biomaterial coatings because they have broad spectrum activity against different microbes, with a low likelihood of incurring antimicrobial resistance. Direct action against the bacterial membrane is the most common mechanism of action (MOA) of AMPs, with specific MOAs d...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.5130774
更新日期:2020-05-26 00:00:00
abstract::The limit of detection of advanced immunoassays, biochips and micro/nano biodetection devices is impacted by the non-specific adsorption of target molecules at the sample surface. In this paper, we present a simple and versatile low cost method for generating active surfaces composed of antibodies arrays surrounded by...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1186/1559-4106-8-37
更新日期:2013-12-01 00:00:00
abstract::The authors investigate membrane composition-mediated interactions between proteins adsorbed onto a two-component lipid bilayer close to critical demixing using coarse-grained molecular dynamics simulations and a phenomenological Ginzburg-Landau theory. The simulations consist of three-bead lipids and platelike protei...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.2977492
更新日期:2008-06-01 00:00:00
abstract::This study presents the morphological and chemical modification of the cell structure of aerosolized Escherichia coli treated with a dielectric barrier discharge (DBD). Exposure to DBD results in severe oxidation of the bacteria, leading to the formation of hydroxyl groups and carbonyl groups and a significant reducti...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4941367
更新日期:2016-03-12 00:00:00
abstract::Not only bacteria but also fungal pathogens, particularly Candida species, can lead to biofilm infections on biomedical devices. By covalent grafting of the antifungal drug caspofungin, which targets the fungal cell wall, onto solid biomaterials, a surface layer can be created that might be able to provide long-term p...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4933108
更新日期:2015-12-14 00:00:00
abstract::Advances in three-dimensional secondary ion mass spectrometry (SIMS) imaging have enabled visualizing the subcellular distributions of various lipid species within individual cells. However, the difficulty of locating organelles using SIMS limits efforts to study their lipid compositions. Here, the authors have assess...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.5019736
更新日期:2018-02-26 00:00:00
abstract::Surface plasmon resonance immunosensor for the detection of bacterial cells was first reported in 1998 with high detection limit as much as 107 cfu/ml. Since then, many efforts have been made aiming to lower the detection limit and improve the sensitivity of detection. The aim of this study was to compare the effect o...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4971270
更新日期:2016-12-06 00:00:00
abstract::Fluorescent nanodiamonds (NDs) coated with therapeutics and cell-targeting structures serve as effective tools for drug delivery. However, NDs circulating in blood can eventually interact with the blood-brain barrier, resulting in undesired pathology. Here, we aimed to detect interaction between NDs and adult brain ti...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/6.0000525
更新日期:2020-12-03 00:00:00
abstract::With the advent of novel fabrication technologies, free-standing poly(l-lactic acid) (PLLA) nanosheets have been shown to have enhanced performance over their micro- or macroscale equivalents as tissue engineering and drug delivery constructs. In the present research, the authors investigated the surface degradation b...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4908206
更新日期:2015-03-20 00:00:00
abstract::Simulations of water at hydrophilic self-assembled monolayer (SAM) surfaces are especially relevant for biological interfaces. Well-defined, atomically smooth surfaces that can be continuously varied are possible with SAMs. These characteristics enable more accurate measurements than many other surfaces with the added...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.2977751
更新日期:2008-09-01 00:00:00
abstract::Magnesium alloys are potential biodegradable implants for biomedical applications, and calcium (Ca) is one kind of ideal element being examined for magnesium alloys and biodegradable ceramic coatings owing to its biocompatibility and mechanical suitability. In this study, microarc oxidation (MAO) coatings were prepare...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.5003320
更新日期:2018-01-16 00:00:00