Fluctuations and destabilization of single phospholipid bilayers.

Abstract:

:Supported phospholipid bilayers are interesting model systems for biologists and present fascinating physical properties. The authors present an extensive experimental study of the dynamic properties of supported bilayers. The structure and the equilibrium properties of single and double supported bilayers were investigated with neutron reflectivity. The submicronic fluctuation spectrum of a nearly free "floating" bilayer was determined using off-specular x-ray scattering: the surface tension of the bilayer, its bending modulus, and the intermembrane potential could be determined. Using fluorescence microscopy, the authors showed that this well-controlled single bilayer can form vesicles. Destabilization occurred either at the main gel-fluid transition of the lipids and could be interpreted in terms of a decrease in the bending rigidity or under an ac low-frequency electric field applied in the fluid phase. In the latter case, the authors also studied the effect of the electric field at the molecular length scale by neutron reflectivity. In both cases, destabilization leads to the formation of relatively monodisperse vesicles. This could give further understanding on the vesicle formation mechanism and on the parameters that determine the vesicle size.

journal_name

Biointerphases

journal_title

Biointerphases

authors

Charitat T,Lecuyer S,Fragneto G

doi

10.1116/1.2936938

subject

Has Abstract

pub_date

2008-06-01 00:00:00

pages

FB3

issue

2

eissn

1934-8630

issn

1559-4106

journal_volume

3

pub_type

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