Nanohybrids consisting of magnetic nanoparticles and gold nanoclusters as effective peroxidase mimics and their application for colorimetric detection of glucose.


:Although protein-stabilized gold nanoclusters (AuNCs) have gathered recent attention as biocompatible peroxidase mimics, their practical utility has been critically limited by the low catalytic activity. Here, the authors have developed a nanohybrid material to significantly enhance the catalytic activity of AuNCs by combining them with other inorganic enzyme mimetics, Fe3O4 magnetic nanoparticles (MNPs), through electrostatic attraction. Owing to the synergistic effect by incorporating AuNCs and MNPs, the constructed nanohybrids yielded highly enhanced catalytic activity and enabled rapid catalytic oxidation of 3,3',5,5'-tetramethylbenzidine substrate to produce a blue-colored solution in proportional to the amount of H2O2. Moreover, a highly sensitive and selective glucose biosensing strategy was developed based on the coupled catalytic action between glucose oxidase and the nanohybrids. Using this method, target glucose was successfully detected in a linear concentration range from 150 to 750 μM with a detection limit as low as 100 μM. Along with excellent linearity, high precision and reproducibility were achieved by employing real human blood serums, which enables its use for the reliable quantification of glucose in practical use. Based on these results, the authors anticipate that the nanohybrids consisting of MNPs and AuNCs can serve as potent peroxidase mimics for the detection of clinically important target molecules.






Cho S,Shin HY,Kim MI




Has Abstract


2017-01-17 00:00:00












  • Burr-like, laser-made 3D microscaffolds for tissue spheroid encagement.

    abstract::The modeling, fabrication, cell loading, and mechanical and in vitro biological testing of biomimetic, interlockable, laser-made, concentric 3D scaffolds are presented. The scaffolds are made by multiphoton polymerization of an organic-inorganic zirconium silicate. Their mechanical properties are theoretically modeled...


    pub_type: 杂志文章


    authors: Danilevicius P,Rezende RA,Pereira FD,Selimis A,Kasyanov V,Noritomi PY,da Silva JV,Chatzinikolaidou M,Farsari M,Mironov V

    更新日期:2015-06-23 00:00:00

  • Measuring cell adhesion forces during the cell cycle by force spectroscopy.

    abstract::Force spectroscopy has been used to measure the adhesion of Saos-2 cells to a glass surface at different phases of the cell cycle. The cells were synchronized in three phases of the cell cycle: G(1), S, and G(2)M. Cells in these phases were compared with unsynchronized and native mitotic cells. Individual cells were a...


    pub_type: 杂志文章


    authors: Weder G,Vörös J,Giazzon M,Matthey N,Heinzelmann H,Liley M

    更新日期:2009-06-01 00:00:00

  • Breaking bonds in the atomic force microscope: extracting information.

    abstract::A theoretical framework is developed to analyze molecular bond breaking in dynamic force spectroscopy using atomic force microscopy (AFM). An analytic expression of the observed bond breaking probability as a function of force is obtained in terms of the relevant physical parameters. Three different experimental reali...


    pub_type: 杂志文章


    authors: Hanke F,Kreuzer HJ

    更新日期:2006-03-01 00:00:00

  • Observation of endoplasmic reticulum tubules via TOF-SIMS tandem mass spectrometry imaging of transfected cells.

    abstract::Advances in three-dimensional secondary ion mass spectrometry (SIMS) imaging have enabled visualizing the subcellular distributions of various lipid species within individual cells. However, the difficulty of locating organelles using SIMS limits efforts to study their lipid compositions. Here, the authors have assess...


    pub_type: 杂志文章


    authors: Chini CE,Fisher GL,Johnson B,Tamkun MM,Kraft ML

    更新日期:2018-02-26 00:00:00

  • Nanotribological characterization of human head hair by friction force microscopy in dry atmosphere and aqueous environment.

    abstract::Friction force microscopy was employed for the tribological investigation of human head hair in two different environments: a dry atmosphere and de-ionized water. The fibers were immobilized by embedding them in indium. The effects of bleaching, conditioning, and immersion in methanolic KOH were quantified in terms of...


    pub_type: 杂志文章


    authors: Nikogeorgos N,Fletcher IW,Boardman C,Doyle P,Ortuoste N,Leggett GJ

    更新日期:2010-06-01 00:00:00

  • Surface engineering of poly(methylmethacrylate): Effects on fluorescence immunoassay.

    abstract::The authors present surface engineering modifications through chemistry of poly(methylmethacrylate) (PMMA) that have dramatic effects on the result of surface-bound fluorescence immunoassays, both for specific and nonspecific signals. The authors deduce the most important effect to be clustering of antibodies on the s...


    pub_type: 杂志文章


    authors: Akers PW,Hoai Le NC,Nelson ARJ,McKenna M,O'Mahony C,McGillivray DJ,Gubala V,Williams DE

    更新日期:2017-06-06 00:00:00

  • Gastric mucus and mucuslike hydrogels: Thin film lubricating properties at soft interfaces.

    abstract::Mucus is a viscous slime that plays a vital role in protecting and lubricating biological tissues, in particular, soft epithelium interfaces such as in the stomach, intestines, and esophagus. Previous attempts to generate mucus models that mimick or simulate its characteristics have been predominantly focused on the r...


    pub_type: 杂志文章


    authors: Røn T,Patil NJ,Ajalloueian F,Rishikesan S,Zappone B,Chronakis IS,Lee S

    更新日期:2017-12-05 00:00:00

  • Assessment of protein entrapment in hydroxyapatite scaffolds by size exclusion chromatography.

    abstract::Although it is well known that the textural properties of scaffolds play an important role in the process of tissue regeneration, the investigation of such effects remain difficult especially at the micro/nano level. Texture confers the material the additional ability to entrap/concentrate molecules circulating in the...


    pub_type: 杂志文章


    authors: Espanol M,Casals I,Lamtahri S,Valderas MT,Ginebra MP

    更新日期:2012-12-01 00:00:00

  • Photonic biosensor based on photocorrosion of GaAs/AlGaAs quantum heterostructures for detection of Legionella pneumophila.

    abstract::Photocorrosion of semiconductors is strongly sensitive to the presence of surface states, and it could be influenced by electrically charged molecules immobilized near the semiconductor/electrolyte interface. The underlying mechanism is related to band bending of the semiconductor structure near the surface and the as...


    pub_type: 杂志文章


    authors: Aziziyan MR,Hassen WM,Morris D,Frost EH,Dubowski JJ

    更新日期:2016-03-22 00:00:00

  • ToF-SIMS and laser-SNMS analysis of Madin-Darby canine kidney II cells with silver nanoparticles using an argon cluster ion beam.

    abstract::The use of nanoparticles is one of the fastest expanding fields in industrial as well as in medical applications, owing to their remarkable characteristics. Silver nanoparticles (AgNPs) are among the most-commercialized nanoparticles because of their antibacterial effects. Laser postionization secondary neutral mass s...


    pub_type: 杂志文章


    authors: Nees R,Pelster A,Körsgen M,Jungnickel H,Luch A,Galla HJ,Arlinghaus HF

    更新日期:2015-06-15 00:00:00

  • Influence of multiscale and curved structures on the migration of stem cells.

    abstract::Understanding how topographical cues can control cell behavior is a major fundamental question which is of particular interest for implant design. Recent findings show that cell-scale curvature, as well as nanoscale topography, can affect different aspects of cell migration. However, the correlation between specific c...


    pub_type: 杂志文章


    authors: Belaud V,Petithory T,Ponche A,Mauclair C,Donnet C,Pieuchot L,Benayoun S,Anselme K

    更新日期:2018-12-31 00:00:00

  • Science and engineering of nanodiamond particle surfaces for biological applications (Review).

    abstract::Diamond has outstanding bulk properties such as super hardness, chemical inertness, biocompatibility, luminescence, to name just a few. In the nanoworld, in order to exploit these outstanding bulk properties, the surfaces of nanodiamond (ND) particles must be accordingly engineered for specific applications. Modificat...


    pub_type: 杂志文章,评审


    authors: Shenderova OA,McGuire GE

    更新日期:2015-09-05 00:00:00

  • Cytoskeletal synchronization of CHO cells with polymer functionalized fullerene C60.

    abstract::Recent developments in the field of fullerene C60 and its derivatives suggest its suitability in a wide range of applications ranging from photovoltaic instruments, development of solar based cells, cosmetics to enzyme inhibition treatment, and so on. These innovative applications raised possibilities of intentional o...


    pub_type: 杂志文章


    authors: Biby ET,Prajitha N,Rajeev KS,Mohanan PV

    更新日期:2019-03-18 00:00:00

  • Grafting of architecture controlled poly(styrene sodium sulfonate) onto titanium surfaces using bio-adhesive molecules: Surface characterization and biological properties.

    abstract::This contribution reports on grafting of bioactive polymers such as poly(sodium styrene sulfonate) (polyNaSS) onto titanium (Ti) surfaces. This grafting process uses a modified dopamine as an anchor molecule to link polyNaSS to the Ti surface. The grafting process combines reversible addition-fragmentation chain trans...


    pub_type: 杂志文章


    authors: Chouirfa H,Evans MDM,Castner DG,Bean P,Mercier D,Galtayries A,Falentin-Daudré C,Migonney V

    更新日期:2017-06-14 00:00:00

  • Simulations of water at the interface with hydrophilic self-assembled monolayers.

    abstract::Simulations of water at hydrophilic self-assembled monolayer (SAM) surfaces are especially relevant for biological interfaces. Well-defined, atomically smooth surfaces that can be continuously varied are possible with SAMs. These characteristics enable more accurate measurements than many other surfaces with the added...


    pub_type: 杂志文章


    authors: Stevens MJ,Grest GS

    更新日期:2008-09-01 00:00:00

  • Biomimetic coatings enhance tribocorrosion behavior and cell responses of commercially pure titanium surfaces.

    abstract::Biofunctionalized surfaces for implants are currently receiving much attention in the health care sector. Our aims were (1) to create bioactive Ti-coatings doped with Ca, P, Si, and Ag produced by microarc oxidation (MAO) to improve the surface properties of biomedical implants, (2) to investigate the TiO2 layer stabi...


    pub_type: 杂志文章


    authors: Marques Ida S,Alfaro MF,Saito MT,da Cruz NC,Takoudis C,Landers R,Mesquita MF,Nociti Junior FH,Mathew MT,Sukotjo C,Barão VA

    更新日期:2016-09-11 00:00:00

  • Morphological and chemical changes of aerosolized E. coli treated with a dielectric barrier discharge.

    abstract::This study presents the morphological and chemical modification of the cell structure of aerosolized Escherichia coli treated with a dielectric barrier discharge (DBD). Exposure to DBD results in severe oxidation of the bacteria, leading to the formation of hydroxyl groups and carbonyl groups and a significant reducti...


    pub_type: 杂志文章


    authors: Romero-Mangado J,Nordlund D,Soberon F,Deane G,Maughan K,Sainio S,Singh G,Daniels S,Saunders IT,Loftus D,Meyyappan M,Koehne J,Gandhiraman RP

    更新日期:2016-03-12 00:00:00

  • Time of flight secondary ion mass spectrometry surface and in-depth study of degradation of nanosheet poly(L-lactic acid) films.

    abstract::With the advent of novel fabrication technologies, free-standing poly(l-lactic acid) (PLLA) nanosheets have been shown to have enhanced performance over their micro- or macroscale equivalents as tissue engineering and drug delivery constructs. In the present research, the authors investigated the surface degradation b...


    pub_type: 杂志文章


    authors: Marchany MD,Gardella JA Jr,Kuchera TJ

    更新日期:2015-03-20 00:00:00

  • Analysis of protein coatings on gold nanoparticles by XPS and liquid-based particle sizing techniques.

    abstract::The precise use of nanoparticles in technological applications requires control over their surface properties. This implies the ability to quantitatively describe, for example, molecular coatings in terms of their thickness, areal mass, or number of molecules. Here, the authors describe two different approaches to the...


    pub_type: 杂志文章


    authors: Belsey NA,Shard AG,Minelli C

    更新日期:2015-03-27 00:00:00

  • X-ray spectromicroscopy of nanoparticulate iron oxide phases.

    abstract::Soft x-ray spectromicroscopy techniques have seen great amount of development in the recent years, and with the development of new diffraction limited synchrotron source, many new nanoscale and mesoscale characterization opportunities of applied materials are foreseen. In this perspective, the authors present some exa...


    pub_type: 杂志文章


    authors: Dietrich PM,Unger WE,Swaraj S

    更新日期:2016-10-28 00:00:00

  • Coated nanodiamonds interact with tubulin beta-III negative cells of adult brain tissue.

    abstract::Fluorescent nanodiamonds (NDs) coated with therapeutics and cell-targeting structures serve as effective tools for drug delivery. However, NDs circulating in blood can eventually interact with the blood-brain barrier, resulting in undesired pathology. Here, we aimed to detect interaction between NDs and adult brain ti...


    pub_type: 杂志文章


    authors: Šimková V,Freislebenová H,Neuhöferová E,Petráková V,Amini A,Benson V

    更新日期:2020-12-03 00:00:00

  • Subpopulations in purified platelets adhering on glass.

    abstract::Understanding how platelet activation is regulated is important in the context of cardiovascular disorders and their management with antiplatelet therapy. Recent evidence points to different platelet subpopulations performing different functions. In particular, procoagulant and aggregating subpopulations have been rep...


    pub_type: 杂志文章


    authors: Donati A,Gupta S,Reviakine I

    更新日期:2016-06-22 00:00:00

  • Instantaneous adhesion of Cuvierian tubules in the sea cucumber Holothuria forskali.

    abstract::The peculiar Cuvierian tubules of sea cucumbers function as a defense mechanism. They thwart attacks by creating a sticky network composed of elongated tubules within which the potential predator is entangled in a matter of seconds and thus immobilized. Cuvierian tubules are typical instantaneous adhesive organs in wh...


    pub_type: 杂志文章


    authors: Demeuldre M,Chinh Ngo T,Hennebert E,Wattiez R,Leclère P,Flammang P

    更新日期:2014-06-01 00:00:00

  • Enhanced osteoblast differentiation on scaffolds coated with TiO2 compared to SiO2 and CaP coatings.

    abstract::The aim was to compare the protein release from normal human osteoblasts (NHO) cultured on scaffolds with similar morphology but different coatings. Different ceramic coatings; TiO(2), SiO(2) and calcium phosphate (CaP); Ca(9)HPO(4)(PO(4))(5)OH, were applied to porous TiO(2) scaffolds prepared by polymer sponge replic...


    pub_type: 杂志文章


    authors: Verket A,Tiainen H,Haugen HJ,Lyngstadaas SP,Nilsen O,Reseland JE

    更新日期:2012-12-01 00:00:00

  • Selective immobilization of biomolecules onto an activated polymeric adlayer.

    abstract::The authors report a facile method for the selective immobilization of biomolecules onto a gold surface that was preactivated by a polymeric adlayer. The polymeric adlayer was designed to perform triple functions: high resistance to nonspecific protein adsorption, efficient surface anchoring, and subsequent covalent a...


    pub_type: 杂志文章


    authors: Lee BS,Park S,Lee KB,Jon S,Choi IS

    更新日期:2007-12-01 00:00:00

  • Reversible changes in cell morphology due to cytoskeletal rearrangements measured in real-time by QCM-D.

    abstract::The mechanical properties and responses of cells to external stimuli (including drugs) are closely connected to important phenomena such as cell spreading, motility, activity, and potentially even differentiation. Here, reversible changes in the viscoelastic properties of surface-attached fibroblasts were induced by t...


    pub_type: 杂志文章


    authors: Tymchenko N,Nilebäck E,Voinova MV,Gold J,Kasemo B,Svedhem S

    更新日期:2012-12-01 00:00:00

  • Biomimetic assembly of polyelectrolyte multilayers containing phosvitin monitored with reflectometric interference spectroscopy.

    abstract::Coatings of biomaterials or implants that facilitate biomineralization possess a great potential for applications focused to the replacement, augmentation, and regeneration of bone tissue. Biomimetic approaches utilize biomolecules for either templating or supporting the crystallization process. One of these promising...


    pub_type: 杂志文章


    authors: Grohmann S,Rothe H,Eisenhuth S,Hoffmann C,Liefeith K

    更新日期:2011-06-01 00:00:00

  • Dynamic cellular uptake of mixed-monolayer protected nanoparticles.

    abstract::Nanoparticles (NPs) are gaining increasing attention for potential application in medicine; consequently, studying their interaction with cells is of central importance. We found that both ligand arrangement and composition on gold nanoparticles play a crucial role in their cellular internalization. In our previous in...


    pub_type: 杂志文章


    authors: Carney RP,Carney TM,Mueller M,Stellacci F

    更新日期:2012-12-01 00:00:00

  • The interaction of marine fouling organisms with topography of varied scale and geometry: a review.

    abstract::Many studies have examined the effects of surface topography on the settlement behaviour of marine organisms and this article reviews these investigations with more emphasis on the effects of topography scale. It has been observed that macro topographies (1-100 mm) are generally favoured by marine fouling taxa and are...


    pub_type: 杂志文章,评审


    authors: Myan FW,Walker J,Paramor O

    更新日期:2013-12-01 00:00:00

  • Charge density and particle size effects on oligonucleotide and plasmid DNA binding to nanosized hydrotalcite.

    abstract::Hydrotalcite (HT) and other layered double metal hydroxides are of great interest as gene delivery and timed release drug delivery systems and as enteric vehicles for biologically active molecules that are sensitive to gastric fluids. HT is a naturally occurring double metal hydroxide that can be synthesized as a nano...


    pub_type: 杂志文章


    authors: Sanderson BA,Sowersby DS,Crosby S,Goss M,Lewis LK,Beall GW

    更新日期:2013-12-01 00:00:00