Abstract:
:Collecting information at the interface between living cells and artificial substrates is exceedingly difficult. The extracellular matrix (ECM) mediates all cell-substrate interactions, and its ordered, fibrillar constituents are organized with nanometer precision. The proceedings at this interface are highly dynamic and delicate. In order to understand factors governing biocompatibility or its counterpart antifouling, it is necessary to probe this interface without disrupting labels or fixation and with sufficient temporal resolution. Here the authors combine nonlinear optical spectroscopy (sum-frequency-generation) and microscopy (second-harmonic-generation), fluorescence microscopy, and quartz crystal microgravimetry with dissipation monitoring in a strategy to elucidate molecular ordering processes in the ECM of living cells. Artificially (fibronectin and collagen I) and naturally ordered ECM fibrils (zebrafish, Danio rerio) were subjected to nonlinear optical analysis and were found to be clearly distinguishable from the background signals of diffusive proteins in the ECM. The initial steps of fibril deposition and ordering were observed in vitro as early as 1 h after cell seeding. The ability to follow the first steps of cell-substrate interactions in spite of the low amount of material present at this interface is expected to prove useful for the assessment of biomedical and environmental interfaces.
journal_name
Biointerphasesjournal_title
Biointerphasesauthors
Diesner MO,Welle A,Kazanci M,Kaiser P,Spatz J,Koelsch Pdoi
10.1116/1.3651142subject
Has Abstractpub_date
2011-12-01 00:00:00pages
171-9issue
4eissn
1934-8630issn
1559-4106journal_volume
6pub_type
杂志文章相关文献
Biointerphases文献大全abstract::In this systematic review, the authors explored the surface aspects of various titanium (Ti) or Ti alloy medical implants, examining the interface formed between the implant and surrounding nonkeratinized soft tissues (periosteum, muscles, tendons, fat, cicatrix, or dura mater). A comprehensive search undertaken in Ju...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.5113607
更新日期:2019-08-16 00:00:00
abstract::The aim was to compare the protein release from normal human osteoblasts (NHO) cultured on scaffolds with similar morphology but different coatings. Different ceramic coatings; TiO(2), SiO(2) and calcium phosphate (CaP); Ca(9)HPO(4)(PO(4))(5)OH, were applied to porous TiO(2) scaffolds prepared by polymer sponge replic...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1007/s13758-012-0036-8
更新日期:2012-12-01 00:00:00
abstract::Inspired by the composition of the native extracellular matrix, biomimetic polyelectrolyte multilayers were assembled from polypeptides and the glycosaminoglycan chondroitin sulfate (CS). To investigate whether peptide conformation imposes an effect on the cell biological functions of osteoblasts, the secondary struct...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1007/s13758-012-0062-6
更新日期:2012-12-01 00:00:00
abstract::The limit of detection of advanced immunoassays, biochips and micro/nano biodetection devices is impacted by the non-specific adsorption of target molecules at the sample surface. In this paper, we present a simple and versatile low cost method for generating active surfaces composed of antibodies arrays surrounded by...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1186/1559-4106-8-37
更新日期:2013-12-01 00:00:00
abstract::The kinetics of adsorption and desorption of two highly asymmetrical model peptides were studied at methyl- and carboxylic acid-terminated alkylthiolate self-assembled monolayer (SAM) surfaces on gold. The model peptides were leucine-lysine (LK), α-helical (LKα14), and β-strand (LKβ15) peptides that have a well-define...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.3494080
更新日期:2010-12-01 00:00:00
abstract::Understanding how platelet activation is regulated is important in the context of cardiovascular disorders and their management with antiplatelet therapy. Recent evidence points to different platelet subpopulations performing different functions. In particular, procoagulant and aggregating subpopulations have been rep...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4953866
更新日期:2016-06-22 00:00:00
abstract::Magnesium alloys are potential biodegradable implants for biomedical applications, and calcium (Ca) is one kind of ideal element being examined for magnesium alloys and biodegradable ceramic coatings owing to its biocompatibility and mechanical suitability. In this study, microarc oxidation (MAO) coatings were prepare...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.5003320
更新日期:2018-01-16 00:00:00
abstract::Living confluent fish fibroblast cells RTG-P1 from rainbow trout adherent on diamond were examined by attenuated total reflection (ATR) infrared (IR) spectroscopy. In particular, IR spectra were recorded dynamically during the adsorption of the cells onto the diamond and during their biochemically induced structural r...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.2710336
更新日期:2007-03-01 00:00:00
abstract::Mucus is a viscous slime that plays a vital role in protecting and lubricating biological tissues, in particular, soft epithelium interfaces such as in the stomach, intestines, and esophagus. Previous attempts to generate mucus models that mimick or simulate its characteristics have been predominantly focused on the r...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.5003708
更新日期:2017-12-05 00:00:00
abstract::The peculiar Cuvierian tubules of sea cucumbers function as a defense mechanism. They thwart attacks by creating a sticky network composed of elongated tubules within which the potential predator is entangled in a matter of seconds and thus immobilized. Cuvierian tubules are typical instantaneous adhesive organs in wh...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4875731
更新日期:2014-06-01 00:00:00
abstract::Cationic amphiphilic peptides have been engineered to target both Gram-positive and Gram-negative bacteria while avoiding damage to other cell types. However, the exact mechanism of how these peptides target, bind, and disrupt bacterial cell membranes is not understood. One specific peptide that has been engineered to...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4982710
更新日期:2017-05-05 00:00:00
abstract::Recent developments in the field of fullerene C60 and its derivatives suggest its suitability in a wide range of applications ranging from photovoltaic instruments, development of solar based cells, cosmetics to enzyme inhibition treatment, and so on. These innovative applications raised possibilities of intentional o...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.5084002
更新日期:2019-03-18 00:00:00
abstract::Bacterial colonies that reside on a surface, known as biofilms, are intrinsically impenetrable to traditional antibiotics, ultimately driving research toward an alternative therapeutic approach. Nitric oxide (NO) has gained attention for its biologically beneficial properties, particularly centered around its antibact...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4962266
更新日期:2016-09-07 00:00:00
abstract::To image inflammation sites, we developed a novel nanoparticle, hydroxylamine-containing nanoparticle (HANP), which emits an intense electron spin resonance (ESR)-signal triggered by enzymatic oxidation reaction and pH-sensitive self-disintegration. The nanoparticle was prepared from an amphiphilic block copolymer, po...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1007/s13758-011-0007-5
更新日期:2012-12-01 00:00:00
abstract::Fluorescent nanodiamonds (NDs) coated with therapeutics and cell-targeting structures serve as effective tools for drug delivery. However, NDs circulating in blood can eventually interact with the blood-brain barrier, resulting in undesired pathology. Here, we aimed to detect interaction between NDs and adult brain ti...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/6.0000525
更新日期:2020-12-03 00:00:00
abstract::In this work, four triple negative (TN) cell lines, three ER+ and PR+ receptor positive (RP) cell lines, and one ER+, PR+, and HER2+ cell line were chemically distinguished from one another using time-of-flight secondary ion mass spectrometry (ToF-SIMS) and principal component analysis (PCA). PCA scores separation was...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4929633
更新日期:2015-06-28 00:00:00
abstract::The surface concentration gradient of two extracellular matrix (ECM) macromolecules was developed to study the migratory and morphological responses of astrocytes to molecular cues typically found in the central nervous system injury environment. The gradient, prepared using microcontact printing, was composed of rand...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.5001675
更新日期:2017-09-11 00:00:00
abstract::Unmodified gelatin (uG) is widely used as a coating material in cell culture for improving surface properties. In this study, the authors investigated if gelatin methacrylamide (GM) with a medium degree of methacrylamide modification (GM1.5) and a high degree of methacrylamide modification (GM4) are equally suitable f...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4949545
更新日期:2016-06-13 00:00:00
abstract::Photolithographic techniques have been used to fabricate polymer brush micro- and nanostructures. On exposure to UV light with a wavelength of 244 nm, halogens were selectively removed from films of chloromethylphenyltrichlorosilane and 3-(2-bromoisobutyramido)propyl-triethoxysilane on silicon dioxide. Patterning was ...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.3553579
更新日期:2011-03-01 00:00:00
abstract::Nanodiamond (ND) particles are popular platforms for the immobilization of molecular species. In the present research, enzyme Escherichia coli inorganic pyrophosphatase (PPase) was immobilized on detonation ND through covalent or noncovalent bonding and its enzymatic activity was characterized. Factors affecting adsor...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4934483
更新日期:2015-12-21 00:00:00
abstract::Hydrotalcite (HT) and other layered double metal hydroxides are of great interest as gene delivery and timed release drug delivery systems and as enteric vehicles for biologically active molecules that are sensitive to gastric fluids. HT is a naturally occurring double metal hydroxide that can be synthesized as a nano...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1186/1559-4106-8-8
更新日期:2013-12-01 00:00:00
abstract::Permanent implants made from titanium are widely used and successfully implemented in medicine to address problems related to orthopedic and oral disorders. However, implants that interact in all cases optimally and durably with bone tissue have yet to be developed. Here, the authors suggest a phospholipids 1-palmitoy...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4938556
更新日期:2015-03-29 00:00:00
abstract::Using infrared and Raman microspectroscopy, the authors examined the interaction of cold atmospheric plasma with the skin's built-in protective cushion, the outermost skin layer stratum corneum. Following a spectroscopic analysis, the authors could identify four prominent chemical alterations caused by plasma treatmen...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4919610
更新日期:2015-06-06 00:00:00
abstract::Diamond has outstanding bulk properties such as super hardness, chemical inertness, biocompatibility, luminescence, to name just a few. In the nanoworld, in order to exploit these outstanding bulk properties, the surfaces of nanodiamond (ND) particles must be accordingly engineered for specific applications. Modificat...
journal_title:Biointerphases
pub_type: 杂志文章,评审
doi:10.1116/1.4927679
更新日期:2015-09-05 00:00:00
abstract::Aqueous stability of sputter coated ZnO thin films were studied on two base materials, viz., polycarbonate (PC) and glass. The films showed higher stability on PC compared to glass, when exposed to aqueous buffered solution at pH-7.4, as studied by x-ray diffraction, surface reflectometry, and inductively coupled plas...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4997011
更新日期:2017-08-02 00:00:00
abstract::Biofunctionalized surfaces for implants are currently receiving much attention in the health care sector. Our aims were (1) to create bioactive Ti-coatings doped with Ca, P, Si, and Ag produced by microarc oxidation (MAO) to improve the surface properties of biomedical implants, (2) to investigate the TiO2 layer stabi...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4960654
更新日期:2016-09-11 00:00:00
abstract::The authors report a facile method for the selective immobilization of biomolecules onto a gold surface that was preactivated by a polymeric adlayer. The polymeric adlayer was designed to perform triple functions: high resistance to nonspecific protein adsorption, efficient surface anchoring, and subsequent covalent a...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.2801974
更新日期:2007-12-01 00:00:00
abstract::A drug-eluting coating applied onto biomedical devices and implants is an appropriate way to ensure that an inhibitory concentration of antimicrobial drugs is present at the device surface, thus preventing surface colonization and subsequent biofilm formation. In this study, a thin polymer coating was applied to mater...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/6.0000511
更新日期:2020-12-18 00:00:00
abstract::Two-dimensional molybdenum disulfide (MoS2) has attracted intense interest owing to its unique properties and promising biosensor applications. To develop effective biocompatible platforms, it is crucial to understand the interactions between MoS2 and biological molecules such as proteins, but little knowledge exists ...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4984803
更新日期:2017-06-02 00:00:00
abstract::Antimicrobial peptides (AMPs) are attractive as biomaterial coatings because they have broad spectrum activity against different microbes, with a low likelihood of incurring antimicrobial resistance. Direct action against the bacterial membrane is the most common mechanism of action (MOA) of AMPs, with specific MOAs d...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.5130774
更新日期:2020-05-26 00:00:00