Live cell adhesion assay with attenuated total reflection infrared spectroscopy.

Abstract:

:Living confluent fish fibroblast cells RTG-P1 from rainbow trout adherent on diamond were examined by attenuated total reflection (ATR) infrared (IR) spectroscopy. In particular, IR spectra were recorded dynamically during the adsorption of the cells onto the diamond and during their biochemically induced structural responses to the subsequent addition of trypsin and cytochalasin D. It is shown that changes in the IR spectra result from changes in cell morphology and surface coverage. The results demonstrate the potential and the applicability of ATR IR spectroscopy for live cell adhesion assays.

journal_name

Biointerphases

journal_title

Biointerphases

authors

Schmidt M,Wolfram T,Rumpler M,Tripp CP,Grunze M

doi

10.1116/1.2710336

subject

Has Abstract

pub_date

2007-03-01 00:00:00

pages

1-5

issue

1

eissn

1934-8630

issn

1559-4106

journal_volume

2

pub_type

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