Abstract:
:Antimicrobial peptides (AMPs) are attractive as biomaterial coatings because they have broad spectrum activity against different microbes, with a low likelihood of incurring antimicrobial resistance. Direct action against the bacterial membrane is the most common mechanism of action (MOA) of AMPs, with specific MOAs dependent on membrane composition, peptide concentration, and environmental factors that include temperature. Chrysophsin-1 (CHY1) is a broad spectrum salt-tolerant AMP that is derived from a marine fish. A cysteine modification was made to the peptide to facilitate attachment to a surface, such as a biomedical device. The authors used quartz crystal microbalance with dissipation monitoring to study how temperature (23 and 37 °C) and lipid composition influence the MOA of cysteine-modified peptide (C-CHY1) with model membranes comprised of supported lipid bilayers (SLBs). These two temperatures were used so that the authors could better understand the differences in behavior between typical lab temperatures and physiologic conditions. The authors created model membranes that mimicked properties of Gram-negative and Gram-positive bacteria in order to understand how the mechanisms might differ for different types of bacterial systems. SLB models of Gram-positive bacterial membranes were formed using combinations of phosphatidylcholine, phosphatidylglycerol (PG), and S. aureus-derived lipoteichoic acid (LTA). SLB models of Gram-negative bacterial membranes were formed using combinations of phosphatidylethanolamine (PE), PG, and E. coli-derived lipopolysaccharides (LPS). The molecules that distinguish Gram-positive and Gram-negative membranes (LTA and LPS) have the potential to alter the MOA of C-CHY1 with the SLBs. The authors' results showed that the MOA for the Gram-positive SLBs was not sensitive to temperature, but the LTA addition did have an effect. Specifically, similar trends in frequency and dissipation changes across all overtones were observed, and the same mechanistic trends were observed in the polar plots at 23 and 37 °C. However, when LTA was added, polar plots showed an association between C-CHY1 and LTA, leading to SLB saturation. This was demonstrated by significant changes in dissipation, while the frequency (mass) was not increasing after the saturation point. For the Gram-negative SLBs, the composition did not have a significant effect on MOA, but the authors saw more differences between the two temperatures studied. The authors believe this is due to the fact that the gel-liquid crystal transition temperature of PE is 25 °C, which means that the bilayer is more rigid at 23 °C, compared to temperatures above the transition point. At 23 °C, a significant energetic shift would be required to allow for additional AMP insertion. This could be seen in the polar plots, where there was a steep slope but there was very little mass addition. At 37 °C, the membrane is more fluid and there is less of an energetic requirement for insertion. Therefore, the authors observed greater mass addition and fewer changes in dissipation. A better understanding of C-CHY1 MOA using different SLB models will allow for the more rational design of future therapeutic solutions that make use of antimicrobial peptides, including those involving biomaterial coatings.
journal_name
Biointerphasesjournal_title
Biointerphasesauthors
Alexander TE,Smith IM,Lipsky ZW,Lozeau LD,Camesano TAdoi
10.1116/1.5130774subject
Has Abstractpub_date
2020-05-26 00:00:00pages
031007issue
3eissn
1934-8630issn
1559-4106journal_volume
15pub_type
杂志文章相关文献
Biointerphases文献大全abstract::Neuronal process growth is guided by extrinsic environmental cues such as extracellular matrix (ECM) proteins. Recent reports have described that the growth cone extension is superior across gradients of the ECM protein laminin compared to growth across uniformly distributed laminin. In this work, the authors have pre...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4857295
更新日期:2014-03-01 00:00:00
abstract::The stable nitroxide radical TEMPO [(2,2,6,6-Tetramethylpiperidin-1-yl)oxyl] has a multitude of applications in fields ranging from energy storage to biomedical applications and many more. However, to date, the processes of incorporating nitroxide radicals into thin-film coatings are laborious and not cost-effective, ...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/6.0000259
更新日期:2020-06-26 00:00:00
abstract::Permanent implants made from titanium are widely used and successfully implemented in medicine to address problems related to orthopedic and oral disorders. However, implants that interact in all cases optimally and durably with bone tissue have yet to be developed. Here, the authors suggest a phospholipids 1-palmitoy...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4938556
更新日期:2015-03-29 00:00:00
abstract::As an antibiotic that prevents and treats infections caused by Gram-positive bacteria such as Staphylococcus aureus, vancomycin incorporated in a biodegradable polymer poly(lactide-co-glycolide) provides opportunities to construct controlled-release drug delivery systems. Developments associated with this promising sy...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.5016186
更新日期:2018-01-10 00:00:00
abstract::Recent developments in the field of fullerene C60 and its derivatives suggest its suitability in a wide range of applications ranging from photovoltaic instruments, development of solar based cells, cosmetics to enzyme inhibition treatment, and so on. These innovative applications raised possibilities of intentional o...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.5084002
更新日期:2019-03-18 00:00:00
abstract::The limit of detection of advanced immunoassays, biochips and micro/nano biodetection devices is impacted by the non-specific adsorption of target molecules at the sample surface. In this paper, we present a simple and versatile low cost method for generating active surfaces composed of antibodies arrays surrounded by...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1186/1559-4106-8-37
更新日期:2013-12-01 00:00:00
abstract::The vascular endothelial cells (EC) extracellular matrix (ECM) on the biomaterial surface can significantly improve the blood compatibility and cell compatibility of the cardiovascular materials. In the present study, two types of micropatterned TiO2 nanotubes surfaces (gronano and toponano) were fabricated on the tit...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4928304
更新日期:2015-12-17 00:00:00
abstract::Diamond has outstanding bulk properties such as super hardness, chemical inertness, biocompatibility, luminescence, to name just a few. In the nanoworld, in order to exploit these outstanding bulk properties, the surfaces of nanodiamond (ND) particles must be accordingly engineered for specific applications. Modificat...
journal_title:Biointerphases
pub_type: 杂志文章,评审
doi:10.1116/1.4927679
更新日期:2015-09-05 00:00:00
abstract::Mucus is a viscous slime that plays a vital role in protecting and lubricating biological tissues, in particular, soft epithelium interfaces such as in the stomach, intestines, and esophagus. Previous attempts to generate mucus models that mimick or simulate its characteristics have been predominantly focused on the r...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.5003708
更新日期:2017-12-05 00:00:00
abstract::With the advent of novel fabrication technologies, free-standing poly(l-lactic acid) (PLLA) nanosheets have been shown to have enhanced performance over their micro- or macroscale equivalents as tissue engineering and drug delivery constructs. In the present research, the authors investigated the surface degradation b...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4908206
更新日期:2015-03-20 00:00:00
abstract::In this work, four triple negative (TN) cell lines, three ER+ and PR+ receptor positive (RP) cell lines, and one ER+, PR+, and HER2+ cell line were chemically distinguished from one another using time-of-flight secondary ion mass spectrometry (ToF-SIMS) and principal component analysis (PCA). PCA scores separation was...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4929633
更新日期:2015-06-28 00:00:00
abstract::Understanding and controlling the performance of engineered nanoparticle (NP) systems is greatly assisted by quantitative characterization of their coatings. Useful measurements methods have been described for NPs in liquid environment, but NP aggregation often represents a limiting factor which impairs the accuracy o...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4968882
更新日期:2016-11-30 00:00:00
abstract::The articular cartilage, once injured, has a limited capacity for intrinsic repair. Preparation of functionally biocartilage substitutes in vitro for cartilage repair is an attractive concept with the recent advances in tissue engineering. In this study, adipose-derived adult stem cells (ADAS) and chondrocytes (Ch) we...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4942754
更新日期:2016-03-25 00:00:00
abstract::Simulations of water at hydrophilic self-assembled monolayer (SAM) surfaces are especially relevant for biological interfaces. Well-defined, atomically smooth surfaces that can be continuously varied are possible with SAMs. These characteristics enable more accurate measurements than many other surfaces with the added...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.2977751
更新日期:2008-09-01 00:00:00
abstract::Fluorescent nanodiamonds (NDs) coated with therapeutics and cell-targeting structures serve as effective tools for drug delivery. However, NDs circulating in blood can eventually interact with the blood-brain barrier, resulting in undesired pathology. Here, we aimed to detect interaction between NDs and adult brain ti...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/6.0000525
更新日期:2020-12-03 00:00:00
abstract::Herein, the authors report and review polyelectrolyte complex (PEC) nanoparticles (NPs) loaded with zoledronate (ZOL) and simvastatin and their effects on bone cells. PEC NPs are intended for modification of bone substitute materials. For characterization, they can be solution casted on germanium (Ge) substrates servi...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4913195
更新日期:2015-03-23 00:00:00
abstract::A theoretical framework is developed to analyze molecular bond breaking in dynamic force spectroscopy using atomic force microscopy (AFM). An analytic expression of the observed bond breaking probability as a function of force is obtained in terms of the relevant physical parameters. Three different experimental reali...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.2188519
更新日期:2006-03-01 00:00:00
abstract::Aqueous stability of sputter coated ZnO thin films were studied on two base materials, viz., polycarbonate (PC) and glass. The films showed higher stability on PC compared to glass, when exposed to aqueous buffered solution at pH-7.4, as studied by x-ray diffraction, surface reflectometry, and inductively coupled plas...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4997011
更新日期:2017-08-02 00:00:00
abstract::The most common bulk acoustic wave device used in biosensing applications is the quartz crystal microbalance (QCM), in which a resonant pure shear acoustic wave is excited via electrodes on both major faces of a thin AT-cut quartz plate. For biosensing, the QCM is used to detect the capture of a target by a target-cap...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/6.0000144
更新日期:2020-06-02 00:00:00
abstract::Nanodiamond (ND) particles are popular platforms for the immobilization of molecular species. In the present research, enzyme Escherichia coli inorganic pyrophosphatase (PPase) was immobilized on detonation ND through covalent or noncovalent bonding and its enzymatic activity was characterized. Factors affecting adsor...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4934483
更新日期:2015-12-21 00:00:00
abstract::The modeling, fabrication, cell loading, and mechanical and in vitro biological testing of biomimetic, interlockable, laser-made, concentric 3D scaffolds are presented. The scaffolds are made by multiphoton polymerization of an organic-inorganic zirconium silicate. Their mechanical properties are theoretically modeled...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4922646
更新日期:2015-06-23 00:00:00
abstract::Solid-supported bilayer lipid membranes (SBLMs) containing membrane protein have been generated through a simple lipid dilution technique. SBLM formation from mixtures of native Escherichia coli bacterial inner membrane (IM) vesicles diluted with egg phosphatidylcholine (egg PC) vesicles has been explored with dissipa...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.2896113
更新日期:2008-06-01 00:00:00
abstract::The authors report on the characterization of the charge formation at supported bilayer lipid membranes (sBLMs) prepared from the zwitterionic lipid 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine on planar silicon dioxide substrates. The charging of the sBLMs was studied in KCl solutions of different ionic strengths be...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.3082042
更新日期:2009-01-01 00:00:00
abstract::Nanoparticles (NPs) are gaining increasing attention for potential application in medicine; consequently, studying their interaction with cells is of central importance. We found that both ligand arrangement and composition on gold nanoparticles play a crucial role in their cellular internalization. In our previous in...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1007/s13758-011-0017-3
更新日期:2012-12-01 00:00:00
abstract::Fast and efficient osseointegration of implants into bone is of crucial importance for their clinical success; a process that can be enhanced by coating the implant surface with hydroxyapatite (HA) using the vacuum plasma spray technology (VPS). However, bacterial infections, especially the biofilm formation on implan...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4943225
更新日期:2016-03-10 00:00:00
abstract::The authors report a facile method for the selective immobilization of biomolecules onto a gold surface that was preactivated by a polymeric adlayer. The polymeric adlayer was designed to perform triple functions: high resistance to nonspecific protein adsorption, efficient surface anchoring, and subsequent covalent a...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.2801974
更新日期:2007-12-01 00:00:00
abstract::Many studies have examined the effects of surface topography on the settlement behaviour of marine organisms and this article reviews these investigations with more emphasis on the effects of topography scale. It has been observed that macro topographies (1-100 mm) are generally favoured by marine fouling taxa and are...
journal_title:Biointerphases
pub_type: 杂志文章,评审
doi:10.1186/1559-4106-8-30
更新日期:2013-12-01 00:00:00
abstract::Peptide nucleic acids (PNAs) are mimics of oligonucleotides containing a neutral peptidelike backbone and are able to bind complementary DNA targets with high affinity and selectivity. In order to investigate the effect of the ionic strength of the buffer solution, hybridization experiments with PNAs as (catcher) prob...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.2746871
更新日期:2007-06-01 00:00:00
abstract::Biofunctionalized surfaces for implants are currently receiving much attention in the health care sector. Our aims were (1) to create bioactive Ti-coatings doped with Ca, P, Si, and Ag produced by microarc oxidation (MAO) to improve the surface properties of biomedical implants, (2) to investigate the TiO2 layer stabi...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4960654
更新日期:2016-09-11 00:00:00
abstract::Magnesium alloys are potential biodegradable implants for biomedical applications, and calcium (Ca) is one kind of ideal element being examined for magnesium alloys and biodegradable ceramic coatings owing to its biocompatibility and mechanical suitability. In this study, microarc oxidation (MAO) coatings were prepare...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.5003320
更新日期:2018-01-16 00:00:00