Abstract:
:Nanodiamond (ND) particles are popular platforms for the immobilization of molecular species. In the present research, enzyme Escherichia coli inorganic pyrophosphatase (PPase) was immobilized on detonation ND through covalent or noncovalent bonding and its enzymatic activity was characterized. Factors affecting adsorption of PPase such as ND size and surface chemistry were studied. The obtained material is a submicron size association of ND particles and protein molecules in approximately equal amounts. Both covalently and noncovalently immobilized PPase retains a significant enzymatic activity (up to 95% of its soluble form) as well as thermostability. The obtained hybrid material has a very high enzyme loading capacity (∼1 mg mg(-1)) and may be considered as a promising delivery system of biologically active proteinaceous substances, particularly in the treatment of diseases such as calcium pyrophosphate crystal deposition disease and related pathologies. They can also be used as recoverable heterogeneous catalysts in the traditional uses of PPase.
journal_name
Biointerphasesjournal_title
Biointerphasesauthors
Rodina EV,Valueva AV,Yakovlev RY,Vorobyeva NN,Kulakova II,Lisichkin GV,Leonidov NBdoi
10.1116/1.4934483subject
Has Abstractpub_date
2015-12-21 00:00:00pages
041005issue
4eissn
1934-8630issn
1559-4106journal_volume
10pub_type
杂志文章相关文献
Biointerphases文献大全abstract::Understanding how topographical cues can control cell behavior is a major fundamental question which is of particular interest for implant design. Recent findings show that cell-scale curvature, as well as nanoscale topography, can affect different aspects of cell migration. However, the correlation between specific c...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.5042747
更新日期:2018-12-31 00:00:00
abstract::Although protein-stabilized gold nanoclusters (AuNCs) have gathered recent attention as biocompatible peroxidase mimics, their practical utility has been critically limited by the low catalytic activity. Here, the authors have developed a nanohybrid material to significantly enhance the catalytic activity of AuNCs by ...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4974198
更新日期:2017-01-17 00:00:00
abstract::Herein, the authors report and review polyelectrolyte complex (PEC) nanoparticles (NPs) loaded with zoledronate (ZOL) and simvastatin and their effects on bone cells. PEC NPs are intended for modification of bone substitute materials. For characterization, they can be solution casted on germanium (Ge) substrates servi...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4913195
更新日期:2015-03-23 00:00:00
abstract::The growing interest in artificial bioorganic interfaces as a platform for applications in emerging areas as personalized medicine, clinical diagnostics, biosensing, biofilms, prevention of biofouling, and other fields of bioengineering is the origin of a need for in detail multitechnique characterizations of such lay...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4948341
更新日期:2016-06-02 00:00:00
abstract::In the present study, we investigated the hydrothermal treatment of titanium with divalent cation solutions and its effect in promoting the adhesion of gingival epithelial cells and fibroblasts in vitro. Gingival keratinocyte-like Sa3 cells or fibroblastic NIH3T3 cells were cultured for 1 h on experimental titanium pl...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1007/s13758-012-0027-9
更新日期:2012-12-01 00:00:00
abstract::Aqueous stability of sputter coated ZnO thin films were studied on two base materials, viz., polycarbonate (PC) and glass. The films showed higher stability on PC compared to glass, when exposed to aqueous buffered solution at pH-7.4, as studied by x-ray diffraction, surface reflectometry, and inductively coupled plas...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4997011
更新日期:2017-08-02 00:00:00
abstract::Shewanella oneidensis MR-1 is a dissimilatory metal-reducing bacterium capable of reducing various metal and sulfur compounds and precipitating them in nanoparticulate form. Here, we report the synthesis of molybdenum disulfide nanomaterials at the site of S. oneidensis biofilms grown in the presence of molybdenum tri...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/6.0000199
更新日期:2020-07-24 00:00:00
abstract::Two-dimensional molybdenum disulfide (MoS2) has attracted intense interest owing to its unique properties and promising biosensor applications. To develop effective biocompatible platforms, it is crucial to understand the interactions between MoS2 and biological molecules such as proteins, but little knowledge exists ...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4984803
更新日期:2017-06-02 00:00:00
abstract::A drug-eluting coating applied onto biomedical devices and implants is an appropriate way to ensure that an inhibitory concentration of antimicrobial drugs is present at the device surface, thus preventing surface colonization and subsequent biofilm formation. In this study, a thin polymer coating was applied to mater...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/6.0000511
更新日期:2020-12-18 00:00:00
abstract::Antimicrobial peptides (AMPs) are attractive as biomaterial coatings because they have broad spectrum activity against different microbes, with a low likelihood of incurring antimicrobial resistance. Direct action against the bacterial membrane is the most common mechanism of action (MOA) of AMPs, with specific MOAs d...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.5130774
更新日期:2020-05-26 00:00:00
abstract::The peculiar Cuvierian tubules of sea cucumbers function as a defense mechanism. They thwart attacks by creating a sticky network composed of elongated tubules within which the potential predator is entangled in a matter of seconds and thus immobilized. Cuvierian tubules are typical instantaneous adhesive organs in wh...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4875731
更新日期:2014-06-01 00:00:00
abstract::Magnesium alloys are potential biodegradable implants for biomedical applications, and calcium (Ca) is one kind of ideal element being examined for magnesium alloys and biodegradable ceramic coatings owing to its biocompatibility and mechanical suitability. In this study, microarc oxidation (MAO) coatings were prepare...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.5003320
更新日期:2018-01-16 00:00:00
abstract::Diamond has outstanding bulk properties such as super hardness, chemical inertness, biocompatibility, luminescence, to name just a few. In the nanoworld, in order to exploit these outstanding bulk properties, the surfaces of nanodiamond (ND) particles must be accordingly engineered for specific applications. Modificat...
journal_title:Biointerphases
pub_type: 杂志文章,评审
doi:10.1116/1.4927679
更新日期:2015-09-05 00:00:00
abstract::In this work, four triple negative (TN) cell lines, three ER+ and PR+ receptor positive (RP) cell lines, and one ER+, PR+, and HER2+ cell line were chemically distinguished from one another using time-of-flight secondary ion mass spectrometry (ToF-SIMS) and principal component analysis (PCA). PCA scores separation was...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4929633
更新日期:2015-06-28 00:00:00
abstract::In this systematic review, the authors explored the surface aspects of various titanium (Ti) or Ti alloy medical implants, examining the interface formed between the implant and surrounding nonkeratinized soft tissues (periosteum, muscles, tendons, fat, cicatrix, or dura mater). A comprehensive search undertaken in Ju...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.5113607
更新日期:2019-08-16 00:00:00
abstract::The aim of this research was to assess the influence of mechanical loading on the ability of Zn-free versus Zn-containing amalgams to promote remineralization at the dentin interface. Sound and caries-affected dentin surfaces (CAD) were restored using Zn-free or Zn-containing dental amalgams. Midcoronal dentin surface...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4933243
更新日期:2015-12-19 00:00:00
abstract::Microneedle technology is one of the attractive methods in transdermal drug delivery. However, the clinical applications of this method are limited owing to: complexity in the preparation of multiple coating solutions, drug leakage while inserting the microneedles into the skin and the outer walls of the solid microne...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4919779
更新日期:2015-06-08 00:00:00
abstract::Using infrared and Raman microspectroscopy, the authors examined the interaction of cold atmospheric plasma with the skin's built-in protective cushion, the outermost skin layer stratum corneum. Following a spectroscopic analysis, the authors could identify four prominent chemical alterations caused by plasma treatmen...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4919610
更新日期:2015-06-06 00:00:00
abstract::Friction force microscopy was employed for the tribological investigation of human head hair in two different environments: a dry atmosphere and de-ionized water. The fibers were immobilized by embedding them in indium. The effects of bleaching, conditioning, and immersion in methanolic KOH were quantified in terms of...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.3432462
更新日期:2010-06-01 00:00:00
abstract::Photocorrosion of semiconductors is strongly sensitive to the presence of surface states, and it could be influenced by electrically charged molecules immobilized near the semiconductor/electrolyte interface. The underlying mechanism is related to band bending of the semiconductor structure near the surface and the as...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4941983
更新日期:2016-03-22 00:00:00
abstract::Biofunctionalized surfaces for implants are currently receiving much attention in the health care sector. Our aims were (1) to create bioactive Ti-coatings doped with Ca, P, Si, and Ag produced by microarc oxidation (MAO) to improve the surface properties of biomedical implants, (2) to investigate the TiO2 layer stabi...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4960654
更新日期:2016-09-11 00:00:00
abstract::Inhalation of combustion-derived ultrafine particles (≤0.1 μm) has been found to be associated with pulmonary and cardiovascular diseases. However, correlation of the physicochemical properties of carbon-based particles such as surface charge and agglomeration state with adverse health effects has not yet been establi...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.5043373
更新日期:2018-09-11 00:00:00
abstract::Dodecin from Halobacterium salinarum is a dodecameric, hollow-spherical protein, which unspecifically adopts flavin molecules. Reduction of flavin dodecin holocomplexes induces dissociation into apododecin and free flavin. Unspecific binding and dissociation upon reduction were used as key properties to construct an e...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.2965134
更新日期:2008-09-01 00:00:00
abstract::The structure, orientation, and formation of amphiphilic alpha-helix model peptide films on fluorocarbon surfaces has been monitored with sum frequency generation (SFG) vibrational spectroscopy, near-edge x-ray absorption fine structure (NEXAFS) spectroscopy, and x-ray photoelectron spectroscopy (XPS). The alpha-helix...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.3317116
更新日期:2010-03-01 00:00:00
abstract::Collecting information at the interface between living cells and artificial substrates is exceedingly difficult. The extracellular matrix (ECM) mediates all cell-substrate interactions, and its ordered, fibrillar constituents are organized with nanometer precision. The proceedings at this interface are highly dynamic ...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.3651142
更新日期:2011-12-01 00:00:00
abstract::Mucus is a viscous slime that plays a vital role in protecting and lubricating biological tissues, in particular, soft epithelium interfaces such as in the stomach, intestines, and esophagus. Previous attempts to generate mucus models that mimick or simulate its characteristics have been predominantly focused on the r...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.5003708
更新日期:2017-12-05 00:00:00
abstract::Biological membranes and their related molecular mechanisms are essential for all living organisms. Membranes host numerous proteins and are responsible for the exchange of molecules and ions, cell signaling, and cell compartmentation. Indeed, the plasma membrane delimits the intracellular compartment from the extrace...
journal_title:Biointerphases
pub_type: 杂志文章,评审
doi:10.1116/1.4994299
更新日期:2017-09-28 00:00:00
abstract::In plants, algae, and cyanobacteria, photosystem 2 (PS2) catalyzes the light driven oxidation of water. The main products of this reaction are protons and molecular oxygen. In vitro, however, it was demonstrated that reactive oxygen species like hydrogen peroxide are obtained as partially reduced side products. The tr...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4938090
更新日期:2015-03-22 00:00:00
abstract::As an antibiotic that prevents and treats infections caused by Gram-positive bacteria such as Staphylococcus aureus, vancomycin incorporated in a biodegradable polymer poly(lactide-co-glycolide) provides opportunities to construct controlled-release drug delivery systems. Developments associated with this promising sy...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.5016186
更新日期:2018-01-10 00:00:00
abstract::Cationic amphiphilic peptides have been engineered to target both Gram-positive and Gram-negative bacteria while avoiding damage to other cell types. However, the exact mechanism of how these peptides target, bind, and disrupt bacterial cell membranes is not understood. One specific peptide that has been engineered to...
journal_title:Biointerphases
pub_type: 杂志文章
doi:10.1116/1.4982710
更新日期:2017-05-05 00:00:00