Measuring cell adhesion forces during the cell cycle by force spectroscopy.

Abstract:

:Force spectroscopy has been used to measure the adhesion of Saos-2 cells to a glass surface at different phases of the cell cycle. The cells were synchronized in three phases of the cell cycle: G(1), S, and G(2)M. Cells in these phases were compared with unsynchronized and native mitotic cells. Individual cells were attached to an atomic force microscope cantilever, brought into brief contact with the glass surface, and then pulled off again. The force-distance curves obtained allowed the work and maximum force of detachment as well as the number, amplitude, and position of discrete unbinding steps to be determined. A statistical analysis of the data showed that the number of binding proteins or protein complexes present at the cell surface and their binding properties remain similar throughout the cell cycle. This, despite the huge changes in cell morphology and adhesion that occur as the cells enter mitosis. These changes are rather associated with the changes in cytoskeletal organization, which can be quantified by force spectroscopy as changes in cell stiffness.

journal_name

Biointerphases

journal_title

Biointerphases

authors

Weder G,Vörös J,Giazzon M,Matthey N,Heinzelmann H,Liley M

doi

10.1116/1.3139962

subject

Has Abstract

pub_date

2009-06-01 00:00:00

pages

27-34

issue

2

eissn

1934-8630

issn

1559-4106

journal_volume

4

pub_type

杂志文章
  • Selective immobilization of biomolecules onto an activated polymeric adlayer.

    abstract::The authors report a facile method for the selective immobilization of biomolecules onto a gold surface that was preactivated by a polymeric adlayer. The polymeric adlayer was designed to perform triple functions: high resistance to nonspecific protein adsorption, efficient surface anchoring, and subsequent covalent a...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.2801974

    authors: Lee BS,Park S,Lee KB,Jon S,Choi IS

    更新日期:2007-12-01 00:00:00

  • Effect of micropatterned TiO2 nanotubes thin film on the deposition of endothelial extracellular matrix: For the purpose of enhancing surface biocompatibility.

    abstract::The vascular endothelial cells (EC) extracellular matrix (ECM) on the biomaterial surface can significantly improve the blood compatibility and cell compatibility of the cardiovascular materials. In the present study, two types of micropatterned TiO2 nanotubes surfaces (gronano and toponano) were fabricated on the tit...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.4928304

    authors: Wu J,Li J,Wu F,He Z,Yang P,Huang N

    更新日期:2015-12-17 00:00:00

  • Titanium surface modifications and their soft-tissue interface on nonkeratinized soft tissues-A systematic review (Review).

    abstract::In this systematic review, the authors explored the surface aspects of various titanium (Ti) or Ti alloy medical implants, examining the interface formed between the implant and surrounding nonkeratinized soft tissues (periosteum, muscles, tendons, fat, cicatrix, or dura mater). A comprehensive search undertaken in Ju...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.5113607

    authors: Zigterman BGR,Van den Borre C,Braem A,Mommaerts MY

    更新日期:2019-08-16 00:00:00

  • Influence of multiscale and curved structures on the migration of stem cells.

    abstract::Understanding how topographical cues can control cell behavior is a major fundamental question which is of particular interest for implant design. Recent findings show that cell-scale curvature, as well as nanoscale topography, can affect different aspects of cell migration. However, the correlation between specific c...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.5042747

    authors: Belaud V,Petithory T,Ponche A,Mauclair C,Donnet C,Pieuchot L,Benayoun S,Anselme K

    更新日期:2018-12-31 00:00:00

  • Role of lipopolysaccharides and lipoteichoic acids on C-Chrysophsin-1 interactions with model Gram-positive and Gram-negative bacterial membranes.

    abstract::Antimicrobial peptides (AMPs) are attractive as biomaterial coatings because they have broad spectrum activity against different microbes, with a low likelihood of incurring antimicrobial resistance. Direct action against the bacterial membrane is the most common mechanism of action (MOA) of AMPs, with specific MOAs d...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.5130774

    authors: Alexander TE,Smith IM,Lipsky ZW,Lozeau LD,Camesano TA

    更新日期:2020-05-26 00:00:00

  • Drug delivery and cell interaction of adhesive poly(ethyleneimine)/sulfated polysaccharide complex particle films.

    abstract::Herein, the authors report and review polyelectrolyte complex (PEC) nanoparticles (NPs) loaded with zoledronate (ZOL) and simvastatin and their effects on bone cells. PEC NPs are intended for modification of bone substitute materials. For characterization, they can be solution casted on germanium (Ge) substrates servi...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.4913195

    authors: Müller M,Torger B,Wehrum D,Vehlow D,Urban B,Woltmann B,Hempel U

    更新日期:2015-03-23 00:00:00

  • On modeling and nanoanalysis of caries-affected dentin surfaces restored with Zn-containing amalgam and in vitro oral function.

    abstract::The aim of this research was to assess the influence of mechanical loading on the ability of Zn-free versus Zn-containing amalgams to promote remineralization at the dentin interface. Sound and caries-affected dentin surfaces (CAD) were restored using Zn-free or Zn-containing dental amalgams. Midcoronal dentin surface...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.4933243

    authors: Toledano M,Aguilera FS,Osorio E,López-López MT,Cabello I,Toledano-Osorio M,Osorio R

    更新日期:2015-12-19 00:00:00

  • Binary self-assembled monolayers modified Au nanoparticles as carriers in biological applications.

    abstract::Gold nanoparticles (AuNPs) are good nonviral carriers because of their ease of synthesis and conjugation in biochemistry, and self-assembled monolayers (SAMs) provide a tunable system to change their interfacial properties. Using homogeneously mixed carboxylic acid and amine functional groups, a series of surface pote...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.4903934

    authors: Chang HY,You YW,Liao HY,Shyue JJ

    更新日期:2014-12-01 00:00:00

  • Critical nitric oxide concentration for Pseudomonas aeruginosa biofilm reduction on polyurethane substrates.

    abstract::Bacterial colonies that reside on a surface, known as biofilms, are intrinsically impenetrable to traditional antibiotics, ultimately driving research toward an alternative therapeutic approach. Nitric oxide (NO) has gained attention for its biologically beneficial properties, particularly centered around its antibact...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.4962266

    authors: Neufeld BH,Reynolds MM

    更新日期:2016-09-07 00:00:00

  • Live cell adhesion assay with attenuated total reflection infrared spectroscopy.

    abstract::Living confluent fish fibroblast cells RTG-P1 from rainbow trout adherent on diamond were examined by attenuated total reflection (ATR) infrared (IR) spectroscopy. In particular, IR spectra were recorded dynamically during the adsorption of the cells onto the diamond and during their biochemically induced structural r...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.2710336

    authors: Schmidt M,Wolfram T,Rumpler M,Tripp CP,Grunze M

    更新日期:2007-03-01 00:00:00

  • ToF-SIMS and laser-SNMS analysis of Madin-Darby canine kidney II cells with silver nanoparticles using an argon cluster ion beam.

    abstract::The use of nanoparticles is one of the fastest expanding fields in industrial as well as in medical applications, owing to their remarkable characteristics. Silver nanoparticles (AgNPs) are among the most-commercialized nanoparticles because of their antibacterial effects. Laser postionization secondary neutral mass s...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.4937466

    authors: Nees R,Pelster A,Körsgen M,Jungnickel H,Luch A,Galla HJ,Arlinghaus HF

    更新日期:2015-06-15 00:00:00

  • Identifying the selectivity of antimicrobial peptides to cell membranes by sum frequency generation spectroscopy.

    abstract::Cationic amphiphilic peptides have been engineered to target both Gram-positive and Gram-negative bacteria while avoiding damage to other cell types. However, the exact mechanism of how these peptides target, bind, and disrupt bacterial cell membranes is not understood. One specific peptide that has been engineered to...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.4982710

    authors: Golbek TW,Franz J,Elliott Fowler J,Schilke KF,Weidner T,Baio JE

    更新日期:2017-05-05 00:00:00

  • Lateral field excited quartz crystal microbalances for biosensing applications.

    abstract::The most common bulk acoustic wave device used in biosensing applications is the quartz crystal microbalance (QCM), in which a resonant pure shear acoustic wave is excited via electrodes on both major faces of a thin AT-cut quartz plate. For biosensing, the QCM is used to detect the capture of a target by a target-cap...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/6.0000144

    authors: Hartz JSR,Emanetoglu NW,Howell C,Vetelino JF

    更新日期:2020-06-02 00:00:00

  • Native E. coli inner membrane incorporation in solid-supported lipid bilayer membranes.

    abstract::Solid-supported bilayer lipid membranes (SBLMs) containing membrane protein have been generated through a simple lipid dilution technique. SBLM formation from mixtures of native Escherichia coli bacterial inner membrane (IM) vesicles diluted with egg phosphatidylcholine (egg PC) vesicles has been explored with dissipa...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.2896113

    authors: Dodd CE,Johnson BR,Jeuken LJ,Bugg TD,Bushby RJ,Evans SD

    更新日期:2008-06-01 00:00:00

  • Comparison of antibody immobilization strategies in detection of Vibrio cholerae by surface plasmon resonance.

    abstract::Surface plasmon resonance immunosensor for the detection of bacterial cells was first reported in 1998 with high detection limit as much as 107 cfu/ml. Since then, many efforts have been made aiming to lower the detection limit and improve the sensitivity of detection. The aim of this study was to compare the effect o...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.4971270

    authors: Taheri RA,Rezayan AH,Rahimi F,Mohammadnejad J,Kamali M

    更新日期:2016-12-06 00:00:00

  • Gelatin methacrylamide as coating material in cell culture.

    abstract::Unmodified gelatin (uG) is widely used as a coating material in cell culture for improving surface properties. In this study, the authors investigated if gelatin methacrylamide (GM) with a medium degree of methacrylamide modification (GM1.5) and a high degree of methacrylamide modification (GM4) are equally suitable f...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.4949545

    authors: Egger M,Tovar GE,Hoch E,Southan A

    更新日期:2016-06-13 00:00:00

  • Dynamic cellular uptake of mixed-monolayer protected nanoparticles.

    abstract::Nanoparticles (NPs) are gaining increasing attention for potential application in medicine; consequently, studying their interaction with cells is of central importance. We found that both ligand arrangement and composition on gold nanoparticles play a crucial role in their cellular internalization. In our previous in...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1007/s13758-011-0017-3

    authors: Carney RP,Carney TM,Mueller M,Stellacci F

    更新日期:2012-12-01 00:00:00

  • X-ray photoelectron spectroscopy characterization of gold nanoparticles functionalized with amine-terminated alkanethiols.

    abstract::Gold nanoparticles (AuNPs) functionalized with a short chain amine-terminated alkanethiol (HS-(CH(2))(2)NH(2) or C2 NH(2)-thiol) are prepared via a direct synthesis method and then ligand-exchanged with a long chain amine-terminated alkanethiol (HS-(CH(2))(11)NH(2) or C11 NH(2)-thiol). Transmission electron microscopy...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.3622481

    authors: Techane SD,Gamble LJ,Castner DG

    更新日期:2011-09-01 00:00:00

  • Reversible changes in cell morphology due to cytoskeletal rearrangements measured in real-time by QCM-D.

    abstract::The mechanical properties and responses of cells to external stimuli (including drugs) are closely connected to important phenomena such as cell spreading, motility, activity, and potentially even differentiation. Here, reversible changes in the viscoelastic properties of surface-attached fibroblasts were induced by t...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1007/s13758-012-0043-9

    authors: Tymchenko N,Nilebäck E,Voinova MV,Gold J,Kasemo B,Svedhem S

    更新日期:2012-12-01 00:00:00

  • Assessment of protein entrapment in hydroxyapatite scaffolds by size exclusion chromatography.

    abstract::Although it is well known that the textural properties of scaffolds play an important role in the process of tissue regeneration, the investigation of such effects remain difficult especially at the micro/nano level. Texture confers the material the additional ability to entrap/concentrate molecules circulating in the...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1007/s13758-012-0037-7

    authors: Espanol M,Casals I,Lamtahri S,Valderas MT,Ginebra MP

    更新日期:2012-12-01 00:00:00

  • Kinetics of leucine-lysine peptide adsorption and desorption at -CH3 and -COOH terminated alkylthiolate monolayers.

    abstract::The kinetics of adsorption and desorption of two highly asymmetrical model peptides were studied at methyl- and carboxylic acid-terminated alkylthiolate self-assembled monolayer (SAM) surfaces on gold. The model peptides were leucine-lysine (LK), α-helical (LKα14), and β-strand (LKβ15) peptides that have a well-define...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.3494080

    authors: Apte JS,Gamble LJ,Castner DG,Campbell CT

    更新日期:2010-12-01 00:00:00

  • Synthesis and characterization of molybdenum disulfide nanoparticles in Shewanella oneidensis MR-1 biofilms.

    abstract::Shewanella oneidensis MR-1 is a dissimilatory metal-reducing bacterium capable of reducing various metal and sulfur compounds and precipitating them in nanoparticulate form. Here, we report the synthesis of molybdenum disulfide nanomaterials at the site of S. oneidensis biofilms grown in the presence of molybdenum tri...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/6.0000199

    authors: Rees JD,Gorby YA,Sawyer SM

    更新日期:2020-07-24 00:00:00

  • Biomimetic coatings enhance tribocorrosion behavior and cell responses of commercially pure titanium surfaces.

    abstract::Biofunctionalized surfaces for implants are currently receiving much attention in the health care sector. Our aims were (1) to create bioactive Ti-coatings doped with Ca, P, Si, and Ag produced by microarc oxidation (MAO) to improve the surface properties of biomedical implants, (2) to investigate the TiO2 layer stabi...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.4960654

    authors: Marques Ida S,Alfaro MF,Saito MT,da Cruz NC,Takoudis C,Landers R,Mesquita MF,Nociti Junior FH,Mathew MT,Sukotjo C,Barão VA

    更新日期:2016-09-11 00:00:00

  • Effect of current density on the microstructure and corrosion resistance of microarc oxidized ZK60 magnesium alloy.

    abstract::The application of magnesium alloys as biomaterials is limited by their poor corrosion behavior. Microarc oxidation (MAO) treatment was used to prepare ceramic coatings on ZK60 magnesium alloys in order to overcome the poor corrosion resistance. The process was conducted at different current densities (3.5 and 9.0 A/d...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.4889734

    authors: You Q,Yu H,Wang H,Pan Y,Chen C

    更新日期:2014-09-01 00:00:00

  • Chemical measurements of polyethylene glycol shells on gold nanoparticles in the presence of aggregation.

    abstract::Understanding and controlling the performance of engineered nanoparticle (NP) systems is greatly assisted by quantitative characterization of their coatings. Useful measurements methods have been described for NPs in liquid environment, but NP aggregation often represents a limiting factor which impairs the accuracy o...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.4968882

    authors: Minelli C,Shard AG

    更新日期:2016-11-30 00:00:00

  • Mixed DNA/oligo(ethylene glycol) functionalized gold surfaces improve DNA hybridization in complex media.

    abstract::Reliable, direct "sample-to-answer" capture of nucleic acid targets from complex media would greatly improve existing capabilities of DNA microarrays and biosensors. This goal has proven elusive for many current nucleic acid detection technologies attempting to produce assay results directly from complex real-world sa...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.2219110

    authors: Lee CY,Gamble LJ,Grainger DW,Castner DG

    更新日期:2006-06-01 00:00:00

  • Burr-like, laser-made 3D microscaffolds for tissue spheroid encagement.

    abstract::The modeling, fabrication, cell loading, and mechanical and in vitro biological testing of biomimetic, interlockable, laser-made, concentric 3D scaffolds are presented. The scaffolds are made by multiphoton polymerization of an organic-inorganic zirconium silicate. Their mechanical properties are theoretically modeled...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.4922646

    authors: Danilevicius P,Rezende RA,Pereira FD,Selimis A,Kasyanov V,Noritomi PY,da Silva JV,Chatzinikolaidou M,Farsari M,Mironov V

    更新日期:2015-06-23 00:00:00

  • Lysozyme orientation and conformation on MoS2 surface: Insights from molecular simulations.

    abstract::Two-dimensional molybdenum disulfide (MoS2) has attracted intense interest owing to its unique properties and promising biosensor applications. To develop effective biocompatible platforms, it is crucial to understand the interactions between MoS2 and biological molecules such as proteins, but little knowledge exists ...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.4984803

    authors: Fan H,Zhao D,Li Y,Zhou J

    更新日期:2017-06-02 00:00:00

  • Immobilization of inorganic pyrophosphatase on nanodiamond particles retaining its high enzymatic activity.

    abstract::Nanodiamond (ND) particles are popular platforms for the immobilization of molecular species. In the present research, enzyme Escherichia coli inorganic pyrophosphatase (PPase) was immobilized on detonation ND through covalent or noncovalent bonding and its enzymatic activity was characterized. Factors affecting adsor...

    journal_title:Biointerphases

    pub_type: 杂志文章

    doi:10.1116/1.4934483

    authors: Rodina EV,Valueva AV,Yakovlev RY,Vorobyeva NN,Kulakova II,Lisichkin GV,Leonidov NB

    更新日期:2015-12-21 00:00:00

  • Platelets and coronary artery disease: Interactions with the blood vessel wall and cardiovascular devices.

    abstract::In view of the rare presence of studies concerning platelet function as risk factor in atherosclerotic patients, processes underlying thromboembolic events are reviewed in this paper. The morphology and the structural organization-membrane receptors, the open canalicular and dense tubular systems, the cytoskeleton, mi...

    journal_title:Biointerphases

    pub_type: 杂志文章,评审

    doi:10.1116/1.4953246

    authors: Reinthaler M,Braune S,Lendlein A,Landmesser U,Jung F

    更新日期:2016-06-08 00:00:00